[Objective] The paper was to study the effect of tobacco blown spot on the yield and output value of tobacco leaf.[Method]The upper,middle and lower leaves in tobacco plant were selected during the harvest period of t...[Objective] The paper was to study the effect of tobacco blown spot on the yield and output value of tobacco leaf.[Method]The upper,middle and lower leaves in tobacco plant were selected during the harvest period of tobacco to carry out loss rate estimation of yield and output value of tobacco leaf caused by different disease levels of brown spot.Regression correlation analysis was also conducted.[Result]The disease levels of brown spot had extremely significant strong negative correlation with single leaf weight of tobacco leaf,and it had extremely significant strong positive correlation with the loss rate of single leaf weight.The increase speed of loss rate of single leaf weight of middle and upper leaves was obviously faster than that of lower leaves.The loss rates of single leaf weight of upper,middle and lower leaves were 3.18%-28.95%,3.43%-28.88% and 10.07%-26.90%,respectively.The higher the disease level of blown spot was,the lower the yield and output value of tobacco leaf was,and the corresponding loss rate was also higher.Correlation analysis showed that the disease level of blown spot had extremely significant strong negative correlation with the yield and output value of tobacco leaf,and it had extremely significant strong positive correlation with the loss rate of yield and output value.The negative impact of blown spot on the output value of tobacco leaf was far greater than that on the yield.The highest loss rate of the yield of tobacco leaf was 28.56%,while the highest loss rate of output value reached 89.67%.[Conclusion] The study provided theoretical basis for accurately holding the critical period for the control of blown spot,thus reducing the damage on tobacco leaf and improving the output value of tobacco leaf.展开更多
Based on three years of long-term fixed-site field trial, the effects of differ- ent tillage and mulching modes on the yield and output value of tobacco and soil quality were investigated. The results showed that the ...Based on three years of long-term fixed-site field trial, the effects of differ- ent tillage and mulching modes on the yield and output value of tobacco and soil quality were investigated. The results showed that the yield and output value of to- bacco in tillage treatment were improved by 22.72 % and 37.23 % compared with non-tillage treatment, respectively; the yield and output value of tobacco in rotation treatment were improved by 1.83% and 19.41% compared with continuous cropping treatment, respectively; the yield and output value of tobacco in straw mulching treatment were improved by 3.55% and 2.4% compared with non-straw mulching treatment, respectively, which indicated that tillage, rotation and straw mulching could improve the yield and output value of tobacco to a certain extent; especially, the yield and output value of tobacco increased significantly after plowing under rotation conditions. The contents of available phosphorus (AP), alkali-hydrolyzable nitrogen (AN), organic matter (OM), total nitrogen (TN) and total phosphorus (TP) in non- tillage treatment were 35.14%, 9.92%, 9.57%, 4.40% and 34.16% higher compared with tillage treatment; especially, under non-tillage conditions, soil pH and contents of available potassium (AK), AP, AN,OM,TN,TP and total potassium (TK) in continu- ous cropping field were 2.01%, 48.68%, 73.09%, 11.45%, 7.71%, 7.31%, 47.68% and 11.78% higher compared with rotation field, indicating that non-tillage treatment and continuous cropping could improve the total content and available content of organic matter, nitrogen and phosphorus. Therefore, from the perspective of soil fer- tility improvement and sustainable tobacco production, continuous cropping under non-tillage conditions might be the most appropriate cropping pattern for local soil fertility improvement; aiming at improving the yield and output value of tobacco, green manure-tobacco→, green manure/wheat/maize→green manure-tobacco ro- tation might be the most appropriate cropping pattern.展开更多
[Objective] The aim of this study was to evaluate the effects of soil amend- ment composed of biochar and inorganic mineral material on growth and develop- ment, yield and output value of flue-cured tobacco in South A...[Objective] The aim of this study was to evaluate the effects of soil amend- ment composed of biochar and inorganic mineral material on growth and develop- ment, yield and output value of flue-cured tobacco in South Anhui Province. [Method] A field plot experiment was conducted. The agronomic traits, yield and output value, as well as appearance quality and flavor of flue-cured tobacco were evaluated. [Re- suit] The soil amendments composed of biochars and inorganic mineral materials could significantly increase plant height, stem diameter, leaf size, yield and output of flue-cured tobacco, as well as proportion of first-grade tobacco leaves at the middle and late growth period. The yield in the X3 treatment group (70% T20 + 30% ZC) was highest, and it was higher than that in the control group by 398 kg/hm2. Com- pared with that in the control group, the output of flue-cured tobacco in the X3 treatment group was increased by 10 290 yuan/hm2. In terms of appearance quality and flavor, the flue-cured tobacco leaves in the soil amendment treatment groups were all better than those in the control group. [Conclusion] The application of soil amendment composed of biochar and inorganic mineral material is an effective mea- sure to improve tobacco-growing soil, promote tobacco growth and development, im- prove tobacco yield and output and improve tobacco leaf quality in South Anhui re- gion.展开更多
[Objective] This study aimed to investigate the effects of the combined use of cake organic fertilizer and tobacco special fertilizer on the growth and development as well as the quality of tobacco leaves.[Method] Dif...[Objective] This study aimed to investigate the effects of the combined use of cake organic fertilizer and tobacco special fertilizer on the growth and development as well as the quality of tobacco leaves.[Method] Different ratios of cake organic fertilizer and tobacco special fertilizer were applied.Then,the agronomic traits and leaf quality determining factors were measured and analyzed at different growing stages.[Result] Compared with the control,the tobacco plant height was increased by 3.01%-5.84%,while the number of valid leaves was increased by 5.56%-20.39% in all the treatments with combined use of fertilizers.The ratio of sugar to nicotine of leaf was closer to 10±1,and the ratio of nitrogen to nicotine of leaf was closer to 1±0.1.In addition,the combined use of 750 kg/hm2 tobacco special base fertilizer and 292 kg/hm2 cake organic fertilizer had a better effect for improving the agronomic traits and flavor qualities than all the other treatments.[Conclusion] This study will provide reference for developing special full-aroma tobacco leaf product and new tobacco special fertilizers in Hunan.展开更多
In order to provide a more suitable environment for tobacco leaf growth, this study collected meteorological information and tobacco leaf quality information from the main tobacco-producing counties in the province an...In order to provide a more suitable environment for tobacco leaf growth, this study collected meteorological information and tobacco leaf quality information from the main tobacco-producing counties in the province and conducted relevant analysis to find out the main meteorological factors affecting the growth, development, yield, and quality of upper tobacco leaves. Clarify the suitable climatic conditions for the growth and development of the upper leaves, and clarify the influence of various meteorological factors on the quality of the upper leaves. The results showed that the nicotine and total nitrogen content of the upper leaves were significantly positively correlated with the average daily temperature during the maturity period, and negatively correlated with the rainfall during the maturity period;the rainfall in July was significantly positively correlated with the upper leaf clutter score, which was sufficient in the mature period. Moisture conditions are conducive to alleviating the upper leaf clutter;the number of sunshine hours in August is the main factor affecting the content of aroma substances in the upper leaves, and it is also significantly positively correlated with the content of aroma substances.展开更多
[Objective] This study was conducted to compare the aroma quality of up-per leaves of K326 flue-cured in different barns, and provide theoretical references for the development and optimization of bulk curing barn and...[Objective] This study was conducted to compare the aroma quality of up-per leaves of K326 flue-cured in different barns, and provide theoretical references for the development and optimization of bulk curing barn and the supporting tech-niques. [Method] The upper leaves of K326 were flue-cured in a commonness barn, a four-shelf bulk curing barn and a three-shelf bulk curing barn separately, and then the contents of aroma components and taste quality indices of the finished leaves were measured and compared. [Result] The contents of total aroma components, chromoplast pigment degradation products, phenylalanine aroma components and neophytadiene showed no significant differences in the leaves flue-cured in four-shelf bulk curing barn and commonness barn, but were significantly or very significantly higher than those in the leaves flue-cured in three-shelf bulk curing barn. The con-tents of carotenoid degradation products, Mail ard reaction products, cembranes aro-ma components and total aroma components (except neophytadiene) showed no significant difference among the three treatments. The results of sensory evaluation revealed that the sensory taste of the leaves flue-cured in four-shelf bulk curing barn was the best, as the finished leaves had rich, delicate aroma, comfortable and clean aftertaste. The leaves flue-cured in commonness barn was better than those flue-cured in three-shelf bulk curing barn in aroma quality, amount and aftertaste. [Conclusion] The four-shelf bulk curing barn had a large volume for tobacco leaf processing, but a similar baking environment to the commonness barn, which is conducive to the formation and accumulation of aroma components, so the finished leaves had similar appearance and quality with those flue-cured in commonness barn. The upper flue-cured leaves in three-shelf bulk curing bran had poorer quality than those flue-cured in the other two barns.展开更多
In order to effectively reduce the chlorophyll content in flue-cured tobacco, improve the overall quality of tobacco leaves, chlorophyllase gene was cloned from Arabidopsis thaliana. After the expression of the expres...In order to effectively reduce the chlorophyll content in flue-cured tobacco, improve the overall quality of tobacco leaves, chlorophyllase gene was cloned from Arabidopsis thaliana. After the expression of the expression vector in E. coil, the recombinant engineering strain was obtained. Afterwards, IPTG (isopropy-β-D-thiogalactopyranoside)was used to induce the goal protein, and the chlorophyllase activity of the recombinant engineering strain was measured, so as to investigate its degradation effect on the chlorophyll in the extracts of tobacco leaves. The results were as follows: (1) the amplified chlorophyllase gene At- CLH1 constructed the expression vector pET28a-AtCLH1 successfully, obtaining the recombinant engineering strain; (2) induced under 30 ℃ for 22 h, the strain could well express the recombinant protein AtCLH1 with 0.5 mmol/L IPTG, and the molecular weight was about 35 kDa; (3) the strain showed good chlorophyllase producing capability, and the activity of the produced chlorophyllase could reach up to 24.9 U/mL, which could degrade the chlorophyll in tobacco extract and had a good application prospect in improving the quality of low quality tobacco; (4) based on the results of orthogonal test, the enzyme extract from the strain was added to the tobacco leaf surface, which could make the degradation rate of chlorophyll in the tobacco leaf reach 17.06% under the temperature of 37 ℃ at the humidity of 75% for 48 h; (5) after treated by the enzyme liquid, the test tobacco showed increase in the content of aromatic substances, enhancement of tobacco fragrance quality and amount, significant decrease of offensive odor and irritation, significant improvement of agreeable aftertaste, making the overall sensory quality of the tobacco leaf significantly improved.展开更多
基金Supported by State Tobacco Monopoly Administration Project "National Survey of Pests in Tobacco" (110200902065)Yunnan Tobacco Monopoly Bureau Technology Project " Investigation of Tobacco Pests in Yunnan Province" (2010YN19)~~
文摘[Objective] The paper was to study the effect of tobacco blown spot on the yield and output value of tobacco leaf.[Method]The upper,middle and lower leaves in tobacco plant were selected during the harvest period of tobacco to carry out loss rate estimation of yield and output value of tobacco leaf caused by different disease levels of brown spot.Regression correlation analysis was also conducted.[Result]The disease levels of brown spot had extremely significant strong negative correlation with single leaf weight of tobacco leaf,and it had extremely significant strong positive correlation with the loss rate of single leaf weight.The increase speed of loss rate of single leaf weight of middle and upper leaves was obviously faster than that of lower leaves.The loss rates of single leaf weight of upper,middle and lower leaves were 3.18%-28.95%,3.43%-28.88% and 10.07%-26.90%,respectively.The higher the disease level of blown spot was,the lower the yield and output value of tobacco leaf was,and the corresponding loss rate was also higher.Correlation analysis showed that the disease level of blown spot had extremely significant strong negative correlation with the yield and output value of tobacco leaf,and it had extremely significant strong positive correlation with the loss rate of yield and output value.The negative impact of blown spot on the output value of tobacco leaf was far greater than that on the yield.The highest loss rate of the yield of tobacco leaf was 28.56%,while the highest loss rate of output value reached 89.67%.[Conclusion] The study provided theoretical basis for accurately holding the critical period for the control of blown spot,thus reducing the damage on tobacco leaf and improving the output value of tobacco leaf.
基金Supported by Study on Sustainable Production and Cultivation System and Nutrient Management for High-quality TobaccoSpecial Fund for Tobacco from Genetic Engineering Project of Sichuan Provincial Department of Finance(2013YCZX-003)~~
文摘Based on three years of long-term fixed-site field trial, the effects of differ- ent tillage and mulching modes on the yield and output value of tobacco and soil quality were investigated. The results showed that the yield and output value of to- bacco in tillage treatment were improved by 22.72 % and 37.23 % compared with non-tillage treatment, respectively; the yield and output value of tobacco in rotation treatment were improved by 1.83% and 19.41% compared with continuous cropping treatment, respectively; the yield and output value of tobacco in straw mulching treatment were improved by 3.55% and 2.4% compared with non-straw mulching treatment, respectively, which indicated that tillage, rotation and straw mulching could improve the yield and output value of tobacco to a certain extent; especially, the yield and output value of tobacco increased significantly after plowing under rotation conditions. The contents of available phosphorus (AP), alkali-hydrolyzable nitrogen (AN), organic matter (OM), total nitrogen (TN) and total phosphorus (TP) in non- tillage treatment were 35.14%, 9.92%, 9.57%, 4.40% and 34.16% higher compared with tillage treatment; especially, under non-tillage conditions, soil pH and contents of available potassium (AK), AP, AN,OM,TN,TP and total potassium (TK) in continu- ous cropping field were 2.01%, 48.68%, 73.09%, 11.45%, 7.71%, 7.31%, 47.68% and 11.78% higher compared with rotation field, indicating that non-tillage treatment and continuous cropping could improve the total content and available content of organic matter, nitrogen and phosphorus. Therefore, from the perspective of soil fer- tility improvement and sustainable tobacco production, continuous cropping under non-tillage conditions might be the most appropriate cropping pattern for local soil fertility improvement; aiming at improving the yield and output value of tobacco, green manure-tobacco→, green manure/wheat/maize→green manure-tobacco ro- tation might be the most appropriate cropping pattern.
基金Supported by Special Project for Shanghai and Anhui Modern Tobacco Agricultural High-tech Demonstration Park(CF56.1-ZJ1)~~
文摘[Objective] The aim of this study was to evaluate the effects of soil amend- ment composed of biochar and inorganic mineral material on growth and develop- ment, yield and output value of flue-cured tobacco in South Anhui Province. [Method] A field plot experiment was conducted. The agronomic traits, yield and output value, as well as appearance quality and flavor of flue-cured tobacco were evaluated. [Re- suit] The soil amendments composed of biochars and inorganic mineral materials could significantly increase plant height, stem diameter, leaf size, yield and output of flue-cured tobacco, as well as proportion of first-grade tobacco leaves at the middle and late growth period. The yield in the X3 treatment group (70% T20 + 30% ZC) was highest, and it was higher than that in the control group by 398 kg/hm2. Com- pared with that in the control group, the output of flue-cured tobacco in the X3 treatment group was increased by 10 290 yuan/hm2. In terms of appearance quality and flavor, the flue-cured tobacco leaves in the soil amendment treatment groups were all better than those in the control group. [Conclusion] The application of soil amendment composed of biochar and inorganic mineral material is an effective mea- sure to improve tobacco-growing soil, promote tobacco growth and development, im- prove tobacco yield and output and improve tobacco leaf quality in South Anhui re- gion.
文摘[Objective] This study aimed to investigate the effects of the combined use of cake organic fertilizer and tobacco special fertilizer on the growth and development as well as the quality of tobacco leaves.[Method] Different ratios of cake organic fertilizer and tobacco special fertilizer were applied.Then,the agronomic traits and leaf quality determining factors were measured and analyzed at different growing stages.[Result] Compared with the control,the tobacco plant height was increased by 3.01%-5.84%,while the number of valid leaves was increased by 5.56%-20.39% in all the treatments with combined use of fertilizers.The ratio of sugar to nicotine of leaf was closer to 10±1,and the ratio of nitrogen to nicotine of leaf was closer to 1±0.1.In addition,the combined use of 750 kg/hm2 tobacco special base fertilizer and 292 kg/hm2 cake organic fertilizer had a better effect for improving the agronomic traits and flavor qualities than all the other treatments.[Conclusion] This study will provide reference for developing special full-aroma tobacco leaf product and new tobacco special fertilizers in Hunan.
文摘In order to provide a more suitable environment for tobacco leaf growth, this study collected meteorological information and tobacco leaf quality information from the main tobacco-producing counties in the province and conducted relevant analysis to find out the main meteorological factors affecting the growth, development, yield, and quality of upper tobacco leaves. Clarify the suitable climatic conditions for the growth and development of the upper leaves, and clarify the influence of various meteorological factors on the quality of the upper leaves. The results showed that the nicotine and total nitrogen content of the upper leaves were significantly positively correlated with the average daily temperature during the maturity period, and negatively correlated with the rainfall during the maturity period;the rainfall in July was significantly positively correlated with the upper leaf clutter score, which was sufficient in the mature period. Moisture conditions are conducive to alleviating the upper leaf clutter;the number of sunshine hours in August is the main factor affecting the content of aroma substances in the upper leaves, and it is also significantly positively correlated with the content of aroma substances.
文摘[Objective] This study was conducted to compare the aroma quality of up-per leaves of K326 flue-cured in different barns, and provide theoretical references for the development and optimization of bulk curing barn and the supporting tech-niques. [Method] The upper leaves of K326 were flue-cured in a commonness barn, a four-shelf bulk curing barn and a three-shelf bulk curing barn separately, and then the contents of aroma components and taste quality indices of the finished leaves were measured and compared. [Result] The contents of total aroma components, chromoplast pigment degradation products, phenylalanine aroma components and neophytadiene showed no significant differences in the leaves flue-cured in four-shelf bulk curing barn and commonness barn, but were significantly or very significantly higher than those in the leaves flue-cured in three-shelf bulk curing barn. The con-tents of carotenoid degradation products, Mail ard reaction products, cembranes aro-ma components and total aroma components (except neophytadiene) showed no significant difference among the three treatments. The results of sensory evaluation revealed that the sensory taste of the leaves flue-cured in four-shelf bulk curing barn was the best, as the finished leaves had rich, delicate aroma, comfortable and clean aftertaste. The leaves flue-cured in commonness barn was better than those flue-cured in three-shelf bulk curing barn in aroma quality, amount and aftertaste. [Conclusion] The four-shelf bulk curing barn had a large volume for tobacco leaf processing, but a similar baking environment to the commonness barn, which is conducive to the formation and accumulation of aroma components, so the finished leaves had similar appearance and quality with those flue-cured in commonness barn. The upper flue-cured leaves in three-shelf bulk curing bran had poorer quality than those flue-cured in the other two barns.
基金Supported by the Planning Project for the Scientific Research and Technological Development of China Tobacco Henan Industrial Co.,Ltd.(ZW201435)
文摘In order to effectively reduce the chlorophyll content in flue-cured tobacco, improve the overall quality of tobacco leaves, chlorophyllase gene was cloned from Arabidopsis thaliana. After the expression of the expression vector in E. coil, the recombinant engineering strain was obtained. Afterwards, IPTG (isopropy-β-D-thiogalactopyranoside)was used to induce the goal protein, and the chlorophyllase activity of the recombinant engineering strain was measured, so as to investigate its degradation effect on the chlorophyll in the extracts of tobacco leaves. The results were as follows: (1) the amplified chlorophyllase gene At- CLH1 constructed the expression vector pET28a-AtCLH1 successfully, obtaining the recombinant engineering strain; (2) induced under 30 ℃ for 22 h, the strain could well express the recombinant protein AtCLH1 with 0.5 mmol/L IPTG, and the molecular weight was about 35 kDa; (3) the strain showed good chlorophyllase producing capability, and the activity of the produced chlorophyllase could reach up to 24.9 U/mL, which could degrade the chlorophyll in tobacco extract and had a good application prospect in improving the quality of low quality tobacco; (4) based on the results of orthogonal test, the enzyme extract from the strain was added to the tobacco leaf surface, which could make the degradation rate of chlorophyll in the tobacco leaf reach 17.06% under the temperature of 37 ℃ at the humidity of 75% for 48 h; (5) after treated by the enzyme liquid, the test tobacco showed increase in the content of aromatic substances, enhancement of tobacco fragrance quality and amount, significant decrease of offensive odor and irritation, significant improvement of agreeable aftertaste, making the overall sensory quality of the tobacco leaf significantly improved.