Lymph node ratio and preoperative CA 19-9 levels predict overall survival and recurrence-free survival in patients with resected pancreatic adenocarcinoma

AIM:Clinicopathologic factors predicting overall survival (OS) would help identify a subset to benefit from adjuvant therapy. METHODS: One hundred and sixty-nine patients patients from 1984 to 2009 with curative resections for pancreatic adenocarcinoma were included. Tumors were staged by American Joint Committee on Cancer 7th edition criteria. Univariate and multivariable analyses were performed using Kaplan-Meier methodology or Cox proportional hazard models. Log-rank tests were performed. Statistical inferences were assessed by two-sided 5% significance level. RESULTS: Median age was 67.1 (57.2-73.0) years with equal gender distribution. Tumors were in the head (89.3%) or body/tail (10.7%). On univariate analysis, adjuvant therapy, lymph node (LN) ratio, histologic grade, negative margin status, absence of peripancreatic extension, and T stage were associated with improved OS. Adjuvant therapy, LN ratio, histologic grade, number of nodes examined, negative LN status, and absence of peripancreatic extension were associated with improved recurrence-free survival (RFS). On multivariable analysis, LN ratio and carbohydrate antigen (CA) 19-9 levels were associated with OS. LN ratio was associated with RFS. CONCLUSION: The LN ratio and CA 19-9 levels are independent prognostic factors following curative resections of pancreatic cancer.

Study on Development of the West of Shandong Province Based on Overall Regional Development Planning Pattern

The overall regional development planning pattern has been considered to be a new and effective pattern as for development of provincial economy.The article analyzed the current conditions of the west of Shandong Province and tried to find the generation mechanisms of current non-balanced economic development of Shandong Province.And then approaches to regional development were put forward according to the theory of regional development pattern as a whole.

The influence of selective cutting of mixed Korean pine(Pinus koraiensis Sieb.et Zucc.) and broad-leaf forest on rare species distribution patterns and spatial correlation in Northeast China

This study aimed to demonstrate change in spatial correlation between Korean pine （Pinus koraiensis Sieb. et Zucc.） and three rare species, and change in spatial distribution of four species in response to a range of selective cutting intensities. We sampled three plots of mixed Korean pine and broad-leaf forest in Lushuihe Forestry Bureau of Jilin province, China. Plot 1, a control, was unlogged Korean pine broad-leaf forest. In plots 2 and 3, Korean pine was selectively cut at 15 and 30 % intensity, respectively, in the 1970s. Other species were rarely cut. We used point-pattern analysis to research the spatial distributions of four tree species and quantify spatial correlations between Korean pine and the other three species, Amur linden （Tilia amurensis Rupr.）, Manchurian ash （Fraxinus mandshurica Rupr.）, and Mongolian oak （Quercus mongolica Fisch.） in all three plots. The results of the study show that selective cutting at 15 % intensity did not significantly change either the species spatial patterns or the spatial correlation between Korean pine and broadleaf species. Selective cutting at 30 % intensity slightly affected the growth of Korean pine and valuable species in forest communities, and the effect was considered nondestructive and recoverable.

Expression Pattern and Target Gene of bbu-miR-103-1 in Buffalo(Bubalus bubalis) at Lactation and Non-lactation Periods

[ Objectivel The paper aimed to investigate the expression pattern of bbu-miR-103-1 in buffalo （Bubalus bubalis） at lactation and non-lactation periods, and to predict its target gene and function. [ Method] Expression pattern of bbu-miR-103-1 at lactation and non-lactation periods were detected by qRT-PCR. The precursor expression plasmid of bbu-miR-103-1 was constructed and named LpEZX-pre-miR-103-1. It was packaged and propagated to produce high-titer lenti- virus in 293T cell lines, which could be used to infect buffalo mammary epithelial cells （BMECs） and over express bbu-miR-103-1. The inhibitor of bbu-miR- 103-1 was chemically synthesized and transfected into BMECs to suppress bbu-miR-103-1 at the same time. The relative expression of pantothenate kinase 3 （ PANK3 ） and milk fat metabolism related genes were detected by qRT-PCR. [ Result] The relative expression of bbu-miR-103-1 at lactation period was 5.29 times higher than that at non-lactation period in buffalo （ P 〈 0.01 ）. The LpEZX-pre-miR-103-1 had been successfully constructed and packaged with the infection titer of 3.47×10^6 PFU/mL. Overexpress or suppress of bbu-miR-103-1 extremely down-regulated or up-regulated the expression level of PANK3 in BMECs （ P 〈 0.01 ）. Over expression of bbu-miR-103~l extremely enhanced the expression of Acetyl-CoA carboxylase alpha（ACACA）, Glycerol-3-phosphate acyhransferase 1 mitochon- drial （GPAM）, Diacylglycerol Oacyhransferase l （DGAT1） and Pyrnvate dehydrogenase lipoamide kinase isozyme 4 （PDK4） （P 〈0.01 ）, and also significantly up-regulated the expression of sterol regulatory element binding protein-1 c （SREBPI c）, Adipose differentiation-related protein （ADFP）, Cluster of differentiation 36 （ CD36）, Acetyl-CoA synthetase short-chain subfamily member 1 （ACSS1） （P 〈0.05）. Over expression of bbu-miR-103-1 down-regulated the expression of PANK3, and improved the mRNA level of SREBPlc by feedback regulation, finally promoting the de novo synthesis of fatty acid beginning with ACACA. [ Conclusion] bbu-miR-103-1 plays an important role in enhancing milk fatty acid synthesis, which provides a molecular base for revealing formation and regulatory mechanism of high-level milk fat in buffalo.

OPTIMIZATION ON ANTENNA PATTERN OF SPACEBORNE SAR WITH IMPROVED NSGA-Ⅱ

Optimization of antenna array pattern used in a spaceborne Synthetic Aperture Radar (SAR) system is considered in this study. A robust evolutionary algorithm, Non-dominated Sorting Genetic Algorithms (the improved NSGA-Ⅱ), is applied on a spaceborne SAR antenna pattern design. The system consists of two objective functions with two constraints. Pareto fronts are generated as a result of multi-objective optimization. After being validated by a test problem ZDT4, the algorithms are used to synthesize spaceborne SAR antenna radiation pattern. The good results with low Ambi- guity-to-Signal Ratio (ASR) and high directivity are obtained in the paper.

Computer-aided Pattern Design of Multi-bar Warp Knitted Fabrics

To meet the development trend of multi-bar warp knit-ting machine towards high-speed,advanced technologyand computer control and the requirements of variousproducts with small quantity,there are many researcheson the computer-aided pattern design of multi-barwarp knitted fabrics.In terms of the special propertiesof the computer-aided pattern design of multi-barwarp knitted fabrics,the Object Oriented Program(OOP)programming-Object Windows class Library(OWL)programming is selected.According to thecharacters of the OWL programming,various functionsare defined.Pattern design and technical parameters canbe output,which offers a great convenience for the fac-tory.

Study On Pattern Design of Men's Tailored-suit Sleeve

Sleeve pattern design of men’ s tailored - suit is one of the most difficult problems of clothing pattern design. Based on the experimental studies of armhole girth, the difference of armhole width and crown height, crown angle, under sleeve curve’s diagonal line length, crown ease and its distribution rule, this paper mainly deals with the sleeve pattern design of tailored - suit for Chinese men. The influences of these factors are discussed and furthermore, some qualitative, quantifiable conclusions are drawn to design perfect pattern of men’ s tailored - suit sleeve.

CD99核旁点状阳性:诊断胰腺实性-假乳头状肿瘤的一种独特的染色模式

Guo Y,Yuan F,Deng H,et al.Paranuclear dot-like immu-nostaining for CD99：a unique staining pattern for diagnosingsolid-pseudopapillary neoplasm of the pancreas.Am J SurgPathol,2011,35（6）：799-806.胰腺实性-假乳头状肿瘤是一种组织发生尚未确定的罕见肿瘤,特征性的表现为假乳头状结构伴囊性和实性生长方式。

CD99核旁逗点状阳性:一种诊断胰腺实性-假乳头状肿瘤的独特的染色模式

Guo Y,Yuan F,Deng H,et al.Paranuclear dot-like immu-nostaining for CD99：A unique staining pattern for diagnosingsolid-pseudopapillary neoplasm of the pancreas.Am J SurgPathol,2011,35：799-806.胰腺实性-假乳头状肿瘤（SPN）,又名乳头状囊性肿瘤。

肿瘤生长形式和肿瘤出芽对Ⅰ-Ⅲ期结直肠癌患者预后的判断价值

目的分析肿瘤生长形式(TGP)和肿瘤出芽(TB)对Ⅰ-Ⅲ期结直肠癌患者预后的判断价值。方法回顾性收集2012年12月-2020年6月于解放军总医院海南医院行根治手术的152例Ⅰ-Ⅲ期结直肠癌患者。通过HE染色判读TGP和TB,并将患者分为TGP-A组(扩张性生长型和中间型)与TGP-B组(侵袭性生长型)或TB低组与TB高组,分析各亚组临床参数的差异,采用Kaplan-Meier法分析TGP-A组与TGP-B组、TB低组与TB高组的无病生存期(DFS)、总生存期(OS);采用Cox比例风险模型分析影响结直肠癌患者预后的危险因素。结果不同肿瘤分化程度、合并N分期在不同TGP、TB亚组中存在统计学差异(P<0.01);此外,CEA值升高(P=0.03)、T3+T4期(P<0.01)、TNMⅢ期(P<0.01)等特征在TGP-B组更明显。TGP-A组与TGP-B组、TB低组与TB高组结直肠癌患者的DFS(TGP:Log-rank=10.06,P<0.01;TB:Log-rank=6.62,P=0.01)、OS(TGP:Log-rank=6.53,P=0.01;TB:Log-rank=4.90,P=0.03)存在统计学差异。TGP是结直肠癌患者DFS(HR=2.95,95%CI 1.54~5.63,P<0.01)和OS(HR=2.37,95%CI 1.14~4.94,P=0.02)的独立危险因素。结论TGP、TB是Ⅰ-Ⅲ期结直肠癌患者较为可靠的预后标志物,TGP表现为侵袭性生长型或TB≥10个/0.785mm2的患者预后较差。

High level of preoperative carbohydrate antigen 19-9 is a poor survival predictor in gastric cancer

AIM:To assess the clinical significance and the prognostic value of preoperative serum carbohydrate antigen 19-9(CA 19-9)level in gastric cancer.METHODS:Between January 2005 and December2006,1960 patients underwent surgery for histologically confirmed gastric cancer.Of these,163 patients had elevated serum levels of CA 19-9 preoperatively,and1628 patients had normal serum levels of CA 19-9 preoperatively.For this study,325 patients were selected from the group of 1628 patients by age,sex,and cancer stage to serve as controls.Statistically significant differences in survival rates were calculated using the log-rank test.A P value less than 0.05 was considered statistically significant and was determined using SAS software.RESULTS:The baseline characteristics showed some differences between the two groups with regard to histology.Overall survival(OS)in the elevated and nonelevated group was 37.90 and 68.67 mo,respectively(P<0.001).N stage(P=0.001)was a significant predictor of disease-free survival by multivariate analysis.Also,N stage(P<0.001),and the presence of peritoneal metastasis(P<0.001)remained independent factors in predicting OS by multivariate analysis.Additionally,preoperative serum CA 19-9 levels were significantly associated with OS in univariate(P=0.009)and multivariate(P=0.021)analyses.CONCLUSION:Serum CA 19-9 can be considered an independent prognostic factor in predicting OS in patients anticipating surgery for gastric cancer.

Prognostic efficacy of inflammation-based markers in patients with curative colorectal cancer resection

AIM To evaluate the prognostic significance of neutrophilto-lymphocyte ratio(NLR), platelet-to-lymphocyte ratio(PLR), lymphocyte-to-monocyte ratio(LMR), and prognostic nutritional index(PNI) and other clinicopathological factors in patients undergoing curative resection of colon cancer.METHODS183 patients with histologically proven colorectal cancer who had undergone potentially curative resection between 2010 and 2016 at Ankara Numune Training and Research Hospital were retrospectively analyzed and clinicopathological characteristics included age, sex, tumor type, grade, size and localization, the number of metastatic and total number of lymph nodes removed, vascular and perineural invasion of the tumor, TNM stages, tumor marker levels(CEA, CA19-9, AFP, CA-125, CA15-3), complete blood counts, albumin levels, overall survival(months), NLR, PLR, LMR and PNI ratios were retrospectively reviewed and analyzed from the electronic database. The primary outcome measure was overall survival.RESULTS Regarding overall survival, on univariate analysis the following variables were significantly associated with poor outcome following resection: T-stage(P = 0.037), lymph node invasion(P = 0.037), cancer stage(P = 0.034), CEA(P = 0.042), CA19-9(P = 0.004), and PNI(P = 0.001). To evaluate the independent prognostic value, multivariate Cox proportional hazard analysis to control for other prognostic factors was used. Using cancer-specific death as an end point for NLR, PLR, LMR, PNI and CA19-9 the optimal cut off values were calculated by ROC analysis. Regarding overall survival, on multivariate analysis high CA19-9(HR = 1.001, 95%CI: 1.00-1.002, P = 0.012) and low PNI(HR = 0.938, 95%CI: 0.891-0.987, P = 0.014) were the only variables independently associated with shortened overall survival. Patients with a PNI < 35 had a median OS of 52.25 mo. In contrast, patients with an PNI > 35 had a median OS of 66 mo. Patients with a CA 19-9 < 17 had a median OS of 66 mo and in patients with a CA19-9 > 17 had a median OS of 53.76 mo.CONCLUSION This study shows that decrease in the PNI and increase in CA 19-9 is associated with poor survival in patients with resectable colon cancer.

Genome-wide analysis of OVATE family proteins in cucumber(Cucumis sativus L.)

OVATE family proteins(OFPs)are plant-specific proteins with a conserved OVATE domain that regulate plant growth and development.Although OFPs have been studied in several species,their biological functions remain largely unknown in cucumber(Cucumis sativus L.).This study identified 19 Cs OFPs distributed on seven chromosomes in cucumber.Most Cs OFP genes were expressed in reproductive organs,but with different expression patterns.Ectopic expression of Cs OFP12-16c in Arabidopsis resulted in shorter and blunt siliques.The overall results indicated that Cs OFP12-16c regulates silique development in Arabidopsis and may have a similar function in cucumber.

Phase Compensation of Composite Material Radomes Based on the Radiation Pattern

Some compensation methods have been pro- posed to mitigate the degradation of radiation characteris- tics caused by composite material radomes, however most of them are complex and not applicable for large radomes, for example, the modification of geometric shape by grinding process. A novel and simple compensation strat- egy based on phase modification is proposed for large reflector antenna-radome systems. Through moving the feed or sub-reflector along axial direction opportunely, the modification of phase distribution in the original aperture of an enclosed reflector antenna can be used to reduce the phase shift caused by composite material radomes. The distortion of far-field pattern can be minimized. The modification formulas are proposed, and the limitation of their application is also discussed. Numerical simulations for a one-piece composite materials sandwich radome and a 40 m multipartite composite materials sandwich radome verify that the novel compensation strategy achieves sat- isfactory compensated results, and improves the distortion of the far-field pattern for the composite material radomes. For one-piece dielectric radome, more than 60% phasedifference caused by radome is reduced. For multipartite radome, the sidelobe level improves about 1.2 dB, the nulling depth improves about 3 dB. The improvement of far-field pattern could be obtained effectively and simply by moving the feed or sub-reflector according to phase shift of the radome.

On the Representation of Multi-layer Woven Structure

A quantitative method is developed to identify the structures of multi-layer woven fabric in this study. Six structural parameters are selected to represent the fabric structure. With some design criteria for the reinforcement of textile composites, the relationships among the structural parameters are derived for different binding patterns of the fabric structure. The conditions, which meet the requirements of structural cell stability,are also discussed.

Study on the Technique of Double-faced Embroidery on Chinese Palace Fans

Double - faced iso - object embroidery is carried out byusing the uneven intercalated stitch crafts of the parallel- lines stitch as a main body, being accompanied by oth-er stitch crafts and miscellaneous stitches, done on thesame textile material. This paper deals with the flower -bird double - faced embroidery fan of the Qing Dynasty,from pattern design, stitching formation and techniqueof color arrangement, describes the possible way of mak-ing double - faced embroidery with iso - object effectboth in the front and in the back.

MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2

Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer progression.However,the roles and biological mechanisms of miRNA-16-5p in human non-small cell lung cancer(NSCLC)are not to be well studied.Here,we validated that the expression of miR-16-5p was decreased significantly in NSCLC samples and cell lines.The correlation between the clinicopathological features of NSCLC and the miR-16-5p expression showed that the expression of miR-16-5p in non-small cell lung cancer was linked with the advanced TNM stage,positive lymph node metastasis,with short overall survival(OS).Also,a negative correlation between miR-16-5p and Fermitin family member 2(FERMT2)was observed,implying there may be a potential link about their regulation.The hypothesis was further confirmed by in-silico analysis and dual-luciferase reporter assay.Moreover,we demonstrated that the transfections of miR-16-5p mimics could alter some biological characteristics of NSCLC cells remarkably accomplished by the expression variance of FERMT2 in vitro and in vivo assays.Summarily,this study demonstrated that miR-16-5p,as a tumor suppression factor in NSCLC by targeting FERMT2,could serve as one promising biomarker in the prediction for NSCLC patients.

Crack patterns corresponding to the residual strength plateau of ceramics subjected to thermal shock

The formation strength plateau of ceramics is addressed. A set of of 99A1203 are conducted, mechanism of the residual subjected to thermal shock thermal shock experiments where the thin specimens of 1 mm× 10 mm×50 mm exhibit parallel through edge cracks, and thus permit quantitative measurements of the crack patterns. The cracks evolve with the severity of ther- mal shock. It is found that there is a correlation between the length and density of the thermal shock cracks. The increase of crack length weakens the residual strength, whereas the increase of crack density improves it. In a considerably wide temperature range, the two contrary effects just counteract each other; consequently a plateau appears in the variation curve of the residual strength. A comparison between the numerical and experimental results of the residual strength is made, and they are found in good agreement. This work is helpful to a deep understanding of the thermal shock failure of ceramics.

整体系统观视域下中国式环境法治现代化的宏观样态与建构方案

作为中国式现代化新道路与生态文明法治交融的产物,中国式环境法治现代化对于整体系统观的理论期许主要表现在应以习近平生态文明法治理论作为根本思想指南、从马克思主义的唯物辩证法中汲取养分以及将“天人合一”的环境伦理观作为其历史脉络三个维度。在宏观样态方面,整体系统观在中国式环境法治现代化中的融入主要体现在绿色低碳理念与经济发展需求深度融合的理念锚定、人际同构型环境利益公平分配格局的目标构筑和“环境权利—环境权力”二元架构均衡配置的范式转变三个场域。在新的历史交汇点上,中国式环境法治现代化应坚持以“整体系统观”作为“元理论”的方法支撑和价值指引,构建彰显环境法治现代化新内涵与新要求、多维度拓展环境法治现代化新道路的有机系统性方案。

建筑、街区与城市的一体化更新探索--以某科技企业上海滨江中心竞赛实践为例

在快速城市化进程中,更新改造几乎伴随着城市发展的全过程。随着时间推移、城市交通格局改变、空间品质提升,很多建筑或地块与城市的关系逐渐呈现碎片、孤立的状态,暴露出土地价值未最大化利用、城市空间未有效激活、公共空间的可达性与连续性不足等问题。如何精准实现城市空间修补、公共空间渗透、文化记忆点睛,提高地块建筑单体与区域整体的品质,是城市更新中重要的课题。文章以笔者从项目策划、国际竞赛至设计总承包全过程实践参与的某科技企业上海滨江中心项目为例,阐述在实践中对建筑、街区与城市一体化更新的探索与思考。