The rates of oxygen consumption, tolerance of hypoxia and desiccation of the Chinese black sleeper ( Bostrichthys sinensis) and mudskipper (Boleophthalmus pectinirostris) embryos were investigated. The pattern of ...The rates of oxygen consumption, tolerance of hypoxia and desiccation of the Chinese black sleeper ( Bostrichthys sinensis) and mudskipper (Boleophthalmus pectinirostris) embryos were investigated. The pattern of oxygen consumption of the Chinese black sleeper embryos was similar to that of the mudskipper ones. The lowest rates of oxygen consumption [ ( 1.65 ±0. 66) nmol/ (ind. ·h) ] of the Chinese black sleeper embryos 16 h after fertilization and the lowest rates of oxygen consumption [ (0.79± 0.08 )nmolf( ind. · h) ] of the mudskipper embryos 6 h after fertilization were recorded, respectively. Then the rates of oxygen consumption of these two species embryos increased gradually until hatching [ (8.26 ± 1.70 ) nmolf( ind.· h) in the Chinese black sleeper, (2.69 ± 0.23 )nmolf( ind. · h) in mudskipper]. After exposure to hypoxia water (0.16 mg/dm^3), bradycardia of the embryos occurred in both the Chinese black sleeper and the mudskipper. However, the Chinese black sleeper embryos survived approximately 45 min longer than the mudskipper ones. After exposure to desiccation at a relative humidity of 58%, bradycardia of the embryos was observed in both the Chinese black sleeper and the mudskipper, and the Chinese black sleeper embryos lived approximately 9 min longer than the mudskipper ones.展开更多
temperature and the diasolved oxygen content affect the oxygen consumption of juveniles of Chinese prawn (Penaeus chinensis), giant tiger prawn (P. monodon) and giant freshwater prawn(Macrobrachium rosenbergii). There...temperature and the diasolved oxygen content affect the oxygen consumption of juveniles of Chinese prawn (Penaeus chinensis), giant tiger prawn (P. monodon) and giant freshwater prawn(Macrobrachium rosenbergii). There is good correlation between the oxygen consumption rate (V, mg/g·h) of the above three prawn species and the water temperature, and dissolved oxygen. In the range of test temperature, V increased with water temperature and diassolved oxygen content. The V of the above three prawn species increased 0.085 mg/g·h, 0. 093 mg/g·h and 0. 08 mg/g·h respectively with each ℃ of rising temperature. The comatose point and stifling point of the juveniles rose obviously at unsuitable temperature.展开更多
The impact of water temperature(24, 27, 30 and 33℃) and salinity(15, 20, 25, 30 and 33) on oxygen consumption(OCR) and ammonium excretion rate(AER) of ♀ Epinephelus fuscoguttatus ×♂ E. lanceolatus hybrid group...The impact of water temperature(24, 27, 30 and 33℃) and salinity(15, 20, 25, 30 and 33) on oxygen consumption(OCR) and ammonium excretion rate(AER) of ♀ Epinephelus fuscoguttatus ×♂ E. lanceolatus hybrid grouper juveniles(9.39 ± 0.07 g) were investigated under the fed and un-fed conditions. The results showed that the OCR and AER were significantly(P < 0.05) affected by temperature and salinity under both fed and un-fed conditions. When temperature was 24–33℃, the OCR and AER of fed hybrid grouper juveniles were 85.68%–129.52% and 125.78%–287.63%, respectively, higher than those of un-fed hybrid grouper juveniles. The O/N ratio, protein use(P_u), Q_(10)(respiration) and Q_(10)(excretion) of fed hybrid grouper juveniles were 14.43–24.01, 28.35% – 48.48%, 1.69 and 3.01, respectively. The O/N ratio, P_u, Q_(10)(respiration), Q_(10)(excretion) of un-fed hybrid grouper juveniles were 20.39 – 31.79, 22.16% – 34.34%, 1.23 and 1.17, respectively. When salinity was 15–33, the OCR and AER of fed hybrid grouper juveniles increased by 87.42% – 116.85% and 215.38% – 353.57%, respectively, over those of un-fed hybrid grouper juveniles. The O/N ratio and P_u of fed hybrid grouper juveniles were 14.48 – 17.78, 39.36% – 49.43%, respectively. The O/N ratio and Pu of un-fed hybrid grouper juveniles were 20.39 – 31.79 and 22.16% – 34.34%, respectively. The specific dynamic action(SDA) of hybrid grouper juveniles was mainly related to protein metabolism. The results had a guiding significance to the large-scale intensive aquaculture of hybrid grouper juveniles.展开更多
Knowledge of oxygen consumption rates and asphyxiation points in fish is important to determine appropriate stocking and water quality management in aquaculture. The oxygen consumption rate and asphyxiation point in C...Knowledge of oxygen consumption rates and asphyxiation points in fish is important to determine appropriate stocking and water quality management in aquaculture. The oxygen consumption rate and asphyxiation point in Chanodichthys mongolicus were detected under laboratory conditions using an improved respirometer chamber. The results revealed that more accurate estimates can be obtained by adjusting the volume of the respirometer chamber, which may avoid system errors caused by either repeatedly adjusting fish density or selecting different equipment specifications. The oxygen consumption rate and asphyxiation point of C. mongolicus increased with increasing water temperature and decreasing fish size. Changes in the C. mongolicus oxygen consumption rate were divided into three stages at water temperatures of 11-33~C: (I) a low temperature oxygen consumption rate stage when water temperature was 11-19~C, (2) the optimum temperature oxygen consumption rate stage when water temperature was 19-23~C, and (3) a high temperature oxygen consumption rate stage when water temperature was 〉27~C. The temperature quotients (Q10) obtained suggested that C. mongolicus preferred a temperature range of 19-23~C. At 19~C, C. mongolicus exhibited higher oxygen consumption rates during the day when the maximum values were observed at 10:00 and 14:00 than at night when the minimum occurred at 02:00.展开更多
Based on heat and mass transfer characteristics of spontaneous combustion of coal,Arrhenius equation and the Ranz-Marshall correlation,a novel approach was proposed in this paper to estimate oxygen consumption rate of...Based on heat and mass transfer characteristics of spontaneous combustion of coal,Arrhenius equation and the Ranz-Marshall correlation,a novel approach was proposed in this paper to estimate oxygen consumption rate of self-ignition of coal at high temperature.Compared with the conventional methods,this approach involves not only kinetic properties of self-ignition of coal and temperature,but also the ambient air flow characteristics and diameter of coal particle.To testify the proposed approach,oxygen consumption rates at high temperature were measured by the programmable isothermal oven experiments.Comparisons between experimental and theoretical results indicate that the rates of oxygen depletion calculated by the proposed approach agree well with those measured from laboratory-scale experiments,which further validates the proposed approach.展开更多
Oxygen consumption is a fundamental component of metabolic networks, mitochondrial function, and global carbon cycling. To date there is no method available that allows for replicate measurements on attached and unatt...Oxygen consumption is a fundamental component of metabolic networks, mitochondrial function, and global carbon cycling. To date there is no method available that allows for replicate measurements on attached and unattached biological samples without compensation for extraneous oxygen leaking into the system. Here we present the Respiratory Detection System, which is compatible with virtually any biological sample. The RDS can be used to measure oxygen uptake in microliter-scale volumes with a reversibly sealed sample chamber, which contains a porphyrin-based oxygen sensor. With the RDS, one can maintain a diffusional seal for up to three hours, allowing for the direct measurement of respiratory function of samples with fast or slow metabolic rates. The ability to easily measure oxygen uptake in small volumes with small populations or dilute samples has implications in cell biology, environmental biology, and clinical diagnostics.展开更多
Biofouling is a particular problem in the pearl oyster culture. It may reduce the growth and survival rates of the cultured species. Foulers are often themselves filter feeders, and are therefore potential competitors...Biofouling is a particular problem in the pearl oyster culture. It may reduce the growth and survival rates of the cultured species. Foulers are often themselves filter feeders, and are therefore potential competitors for food resource with the cultured species. Fouling organisms may also reduce the oxygen supply. A study was conducted to measure the impact of foulers on feeding oxygen consumption, and waste excretion. POM, ammonia, phosphate and oxygen concentration were measured in various treatments (cultured species, foulers). This study showed that fouling organisms had significant effect on food uptake, oxygen consumption and waste excretion. The clearance rate, ammonia and phosphate release rate, oxygen consumption rate of the fouled pearl oyster were greater than those of the clean pearl oysters. Other foulers that settled on cages or buoys also contributed much to phytoplankton depletion, oxygen consumption and concentration increase of ammonia and phosphate in water. Therefore, this study showed us that foulers were important competitors in the pearl oyster cultivation of Daya Bay in November 2005.展开更多
Effects of temperatures and salinities on oxygen consumption and ammonia-N excretion rate of clam Meretrix meretrix were studied in laboratory from Oct. 2003 to Jan. 2004. Two schemes were designed in incremented temp...Effects of temperatures and salinities on oxygen consumption and ammonia-N excretion rate of clam Meretrix meretrix were studied in laboratory from Oct. 2003 to Jan. 2004. Two schemes were designed in incremented temperature at 10, 15, 20, 25℃ at 31.5 salinity and in incremented salinity at16.0, 21.0, 26.0, 31.5, 36.0, and 41.0 at 20℃, all for 8-10 days. From 10 to 25℃, both respiration and excretion rate were increased. One-way ANOVA analysis demonstrated significant difference (P〈0.01) in physiological parameters in this temperature range except between 15 and 20℃. The highest Q10 thermal coefficient value (12.27) was acquired between 10 and 15℃, and about 1 between 15 and 20℃, indicating M. meretrix could well acclimate to temperature changes in this range. Salinity also had significant effects on respiration and excretion rate (P〈0.05). The highest values of respiration and excretion rate of M. meretrix were recorded at 16.0 salinity (20℃). These two physiological parameters decreased as salinity increased until reached the minimum Q10 value at 31.5 (20℃), then again, these parameters increased with increasing salinity from 31.5 to 41.0. M. meretrix can catabolize body protein to cope with osmotic pressure stress when environmental salinity is away from its optimal range. No significant difference was observed between 26.0 and 36.0 in salinity (P〉0.05), suggesting that a best metabolic salinity range for this species is between 26.0 and 36.0.展开更多
To investigate sulfide detoxification in Urech& unicinctus, oxygen consumption rate and sulfide detoxification products were analyzed during sulfide exposure under controlled laboratory conditions. The results showed...To investigate sulfide detoxification in Urech& unicinctus, oxygen consumption rate and sulfide detoxification products were analyzed during sulfide exposure under controlled laboratory conditions. The results showed that oxygen consumption rates were elevated significantly during 3 h sulfide exposure compared to the control (P〈0.05). The concentration of sulfite in body wall and hindgut of experimental worms increased significantly (P〈0.05) when exposed to 50 μmolL^-1 sulfide, reached a maximum at 24 h and then decreased. Similar result was observed in worms exposed to 150 μmolL^-1 sulfide except that sulfite concentration reached a maximum at 12 h. Contents of thiosulfate in body wall and hindgut of U. unicinctus exposed to sulfide were also significantly higher than that of the control without sulfide exposure. In conclusion, during short-time sulfide exposure U. unicinctus may raise oxygen consumption to detoxify toxic sulfide into sulfite and thiosulfate. Sulfide detoxification was restrained when the exposure time was prolonged or sulfide concentration was increased, which was indicated by decrease of sulfite, the intermediate product of sulfide detoxification.展开更多
Effects of reduced salinity on the oxygen consumption rate (OCR) and the ammonia N excretion rate (AER) of scallop Chlamys farreri (3.2 5.9 cm in shell height, 0.147 1.635 g in soft tissue dry weight ) were studied in...Effects of reduced salinity on the oxygen consumption rate (OCR) and the ammonia N excretion rate (AER) of scallop Chlamys farreri (3.2 5.9 cm in shell height, 0.147 1.635 g in soft tissue dry weight ) were studied in laboratory from March 21,1997 to April 16, 1997. Under the controlled conditions of reduced salinity from 31.5 to 15.0 and ambient temperature 17℃ and 23℃, the concentrations of dissolved oxygen and ammonia N were determined by the Winkle method and the hypobromite method, respectively. Results showed that with controlled reduced salinity, the mean values of the OCR were 2.17 mg/(g.h) at 17℃, and 2.86 mg/(g.h) at 23℃and that the mean values of the AER were 178.0 μg/(g.h) at 17℃ and 147.0 μg/(g.h) at 23℃. The OCR and the AER decreased with reducing salinity from 31.5 to 15.0 both at 17℃ and 23℃. The effects of reduced salinity on the OCR and the AER of scallop C. farreri could be represented by the allometric equation and the exponential equation, respectively.展开更多
[Objective]The study aimed to determine oxygen consumption of Erinaceus europaeus at different temperatures to accumulate basic da- ta for the protection and deep discuss of Erinaceus europaeus. [Method]By using the i...[Objective]The study aimed to determine oxygen consumption of Erinaceus europaeus at different temperatures to accumulate basic da- ta for the protection and deep discuss of Erinaceus europaeus. [Method]By using the improved close respiration apparatus,the time spent by ani- mals consuming a unit volume of oxygen was recorded by the improved close respiration apparatus to estimate oxygen consumption of Erinaceus europaeus at 20,25 and 30 °C respectively. [Result]When the temperature varied from 20 to 30 °C,the oxygen consumption and oxygen con- sumption rate of Erinaceus europaeus reduced with the increase of external temperature on the whole. The weight of Erinaceus europaeus had no obvious relation with its oxygen consumption and oxygen consumption rate. In addition,the temperature had linear regression relationships with the oxygen consumption and oxygen consumption rate of Erinaceus europaeus,that is,the linear regression equation between oxygen consumption ( x 0 ) ( ml/h) and temperature ( t) was x 0 =658. 81 -14. 98t,and the equation between oxygen consumption rate ( Q 0 ) [L/( kg·h) ]and tempera- ture ( t) was Q 0 =827. 94 -19. 52t. [Conclusion]The metabolic rate of Erinaceus europaeus changed with environmental temperature,and 20 °C was more suitable for its growth,conforming with the fact that it is active just at night.展开更多
The effects of water temperature on oxygen consumption rate and ammonia excretion rate of Solenaia oleivora were studied in the laboratory. The results showed that, under controlled conditions and ambient temperatures...The effects of water temperature on oxygen consumption rate and ammonia excretion rate of Solenaia oleivora were studied in the laboratory. The results showed that, under controlled conditions and ambient temperatures 15—30℃, the relationship between oxygen consumption rate (O) [mg/h] and dry weight of soft tissue (W) [g] can be represented by an allometric equation O=aW b, while the relationship between ammonia excretion rate (N) [μg/h] and dry weight of soft tissue (W) [g] follows also an allometric equation N=cW b. It is indicated that both metabolic rates are correlated positively with water temperature. High temperature can reduce the level of protein metabolism. The linear regression among oxygen consumption rate (O), temperature (T) and dry weight of soft tissue (W) can be described by the equation O=-0.6513+0.0532T+0.1073W, and for ammonia this relation is N=32.1626-1.0566T+1.3222W, the multiple relation coefficient was 0.9642 and 0.8921, respectively.展开更多
of temperature on oxygen consumption rate (OCR) and ammonia-N excretion rate of scallop Chlamys farreri (1.7-6.2 cm in shell height) were studied in laboratory from Dec. 30 1996 to Jan. 28, 1997 . Under the controlled...of temperature on oxygen consumption rate (OCR) and ammonia-N excretion rate of scallop Chlamys farreri (1.7-6.2 cm in shell height) were studied in laboratory from Dec. 30 1996 to Jan. 28, 1997 . Under the controlled conditions of ambient water temperature 10-31℃ and salinity 32, the concentrations of dissolved oxygen and ammonia-N were determined by the Winkle method and the hypobromite method , respectively . Results showed that the OCR ranged from 1.20 mg/g (DW). h to 5.76mg/g (DW). h. The OCR increased with temperature from 10℃ to 23℃, but at 28℃ the OCR of mature individuals decreased, and that of different size scallops reduced at 31℃. The ammonia-N excretion rate ranged from 113.13 μg NH4-N/g (DW). h to 486.63 μg NH4 -N/g (DW). h,and increased with temperature from 10℃ to 31℃.展开更多
Ridgetail white prawn (Exopalaemon carinicauda) are of significant economic importance in China where they are widely cultured. However, there is little information on the basic biology of this species. We evaluated...Ridgetail white prawn (Exopalaemon carinicauda) are of significant economic importance in China where they are widely cultured. However, there is little information on the basic biology of this species. We evaluated the effect of temperature (16, 19, 22, 25, 28, 31, and 34℃) on the standard metabolic rates (SMRs) of juvenile and adult E. carinicauda in the laboratory under static conditions. The oxygen consumption rate (OCR), ammonia-N excretion rate (AER), and atornic ratio of oxygen consumed to nitrogen consumed (O:N ratio) of juvenile and adult E. carinicauda were significantly influenced by temperature (P〈0.05). Both the OCR and AER of juveniles increased significantly with increasing temperature from 16 to 34℃, but the maximum OCR for adults was at 31℃. Juvenile shrimp exhibited a higher OCR than the adults from 19 to 34℃. There was no significant difference between the AERs of the two life-stages from 16 to 31 ℃ (P〉0.05). The O:N ratio in juveniles was significantly higher than that in the adults over the entire temperature range (P〈0.05). The temperature coefficient (Q_10) of OCR and AER ranged from 5.03 to 0.86 and 6,30 to 0.85 for the adults, respectively, and from 6,09-1.03 and 3.66-1.80 for the juveniles, respectively. The optimal temperature range for growth of the juvenile and adult shrimp was from 28 to 31℃, based on Q_10 and SMR values. Results from the present study may be used to guide pond culture production ofE. carinicauda.展开更多
基金supported by the National Natural Science Foundation of China under contract No.40476056the Science and Technology Key Project of Fujian Province of China under contract Nos 2003N026 and 2004SZ01-02.
文摘The rates of oxygen consumption, tolerance of hypoxia and desiccation of the Chinese black sleeper ( Bostrichthys sinensis) and mudskipper (Boleophthalmus pectinirostris) embryos were investigated. The pattern of oxygen consumption of the Chinese black sleeper embryos was similar to that of the mudskipper ones. The lowest rates of oxygen consumption [ ( 1.65 ±0. 66) nmol/ (ind. ·h) ] of the Chinese black sleeper embryos 16 h after fertilization and the lowest rates of oxygen consumption [ (0.79± 0.08 )nmolf( ind. · h) ] of the mudskipper embryos 6 h after fertilization were recorded, respectively. Then the rates of oxygen consumption of these two species embryos increased gradually until hatching [ (8.26 ± 1.70 ) nmolf( ind.· h) in the Chinese black sleeper, (2.69 ± 0.23 )nmolf( ind. · h) in mudskipper]. After exposure to hypoxia water (0.16 mg/dm^3), bradycardia of the embryos occurred in both the Chinese black sleeper and the mudskipper. However, the Chinese black sleeper embryos survived approximately 45 min longer than the mudskipper ones. After exposure to desiccation at a relative humidity of 58%, bradycardia of the embryos was observed in both the Chinese black sleeper and the mudskipper, and the Chinese black sleeper embryos lived approximately 9 min longer than the mudskipper ones.
文摘temperature and the diasolved oxygen content affect the oxygen consumption of juveniles of Chinese prawn (Penaeus chinensis), giant tiger prawn (P. monodon) and giant freshwater prawn(Macrobrachium rosenbergii). There is good correlation between the oxygen consumption rate (V, mg/g·h) of the above three prawn species and the water temperature, and dissolved oxygen. In the range of test temperature, V increased with water temperature and diassolved oxygen content. The V of the above three prawn species increased 0.085 mg/g·h, 0. 093 mg/g·h and 0. 08 mg/g·h respectively with each ℃ of rising temperature. The comatose point and stifling point of the juveniles rose obviously at unsuitable temperature.
基金supported by the project of National Science and Technology Supporting Plan (No. 2011BAD13B04)
文摘The impact of water temperature(24, 27, 30 and 33℃) and salinity(15, 20, 25, 30 and 33) on oxygen consumption(OCR) and ammonium excretion rate(AER) of ♀ Epinephelus fuscoguttatus ×♂ E. lanceolatus hybrid grouper juveniles(9.39 ± 0.07 g) were investigated under the fed and un-fed conditions. The results showed that the OCR and AER were significantly(P < 0.05) affected by temperature and salinity under both fed and un-fed conditions. When temperature was 24–33℃, the OCR and AER of fed hybrid grouper juveniles were 85.68%–129.52% and 125.78%–287.63%, respectively, higher than those of un-fed hybrid grouper juveniles. The O/N ratio, protein use(P_u), Q_(10)(respiration) and Q_(10)(excretion) of fed hybrid grouper juveniles were 14.43–24.01, 28.35% – 48.48%, 1.69 and 3.01, respectively. The O/N ratio, P_u, Q_(10)(respiration), Q_(10)(excretion) of un-fed hybrid grouper juveniles were 20.39 – 31.79, 22.16% – 34.34%, 1.23 and 1.17, respectively. When salinity was 15–33, the OCR and AER of fed hybrid grouper juveniles increased by 87.42% – 116.85% and 215.38% – 353.57%, respectively, over those of un-fed hybrid grouper juveniles. The O/N ratio and P_u of fed hybrid grouper juveniles were 14.48 – 17.78, 39.36% – 49.43%, respectively. The O/N ratio and Pu of un-fed hybrid grouper juveniles were 20.39 – 31.79 and 22.16% – 34.34%, respectively. The specific dynamic action(SDA) of hybrid grouper juveniles was mainly related to protein metabolism. The results had a guiding significance to the large-scale intensive aquaculture of hybrid grouper juveniles.
基金Supported by the Central-Level Non-Profit Scientific Research Institutes Special Funds(Nos.2014A07XK04,HSY201403)the Harbin Science and Technology Project(No.2012AA6CN037)the National Key Technology Research and Development Program of China(No.2012BAD25B09)
文摘Knowledge of oxygen consumption rates and asphyxiation points in fish is important to determine appropriate stocking and water quality management in aquaculture. The oxygen consumption rate and asphyxiation point in Chanodichthys mongolicus were detected under laboratory conditions using an improved respirometer chamber. The results revealed that more accurate estimates can be obtained by adjusting the volume of the respirometer chamber, which may avoid system errors caused by either repeatedly adjusting fish density or selecting different equipment specifications. The oxygen consumption rate and asphyxiation point of C. mongolicus increased with increasing water temperature and decreasing fish size. Changes in the C. mongolicus oxygen consumption rate were divided into three stages at water temperatures of 11-33~C: (I) a low temperature oxygen consumption rate stage when water temperature was 11-19~C, (2) the optimum temperature oxygen consumption rate stage when water temperature was 19-23~C, and (3) a high temperature oxygen consumption rate stage when water temperature was 〉27~C. The temperature quotients (Q10) obtained suggested that C. mongolicus preferred a temperature range of 19-23~C. At 19~C, C. mongolicus exhibited higher oxygen consumption rates during the day when the maximum values were observed at 10:00 and 14:00 than at night when the minimum occurred at 02:00.
基金Project(51534008) supported by the National Natural Science Foundation of China
文摘Based on heat and mass transfer characteristics of spontaneous combustion of coal,Arrhenius equation and the Ranz-Marshall correlation,a novel approach was proposed in this paper to estimate oxygen consumption rate of self-ignition of coal at high temperature.Compared with the conventional methods,this approach involves not only kinetic properties of self-ignition of coal and temperature,but also the ambient air flow characteristics and diameter of coal particle.To testify the proposed approach,oxygen consumption rates at high temperature were measured by the programmable isothermal oven experiments.Comparisons between experimental and theoretical results indicate that the rates of oxygen depletion calculated by the proposed approach agree well with those measured from laboratory-scale experiments,which further validates the proposed approach.
文摘Oxygen consumption is a fundamental component of metabolic networks, mitochondrial function, and global carbon cycling. To date there is no method available that allows for replicate measurements on attached and unattached biological samples without compensation for extraneous oxygen leaking into the system. Here we present the Respiratory Detection System, which is compatible with virtually any biological sample. The RDS can be used to measure oxygen uptake in microliter-scale volumes with a reversibly sealed sample chamber, which contains a porphyrin-based oxygen sensor. With the RDS, one can maintain a diffusional seal for up to three hours, allowing for the direct measurement of respiratory function of samples with fast or slow metabolic rates. The ability to easily measure oxygen uptake in small volumes with small populations or dilute samples has implications in cell biology, environmental biology, and clinical diagnostics.
文摘Biofouling is a particular problem in the pearl oyster culture. It may reduce the growth and survival rates of the cultured species. Foulers are often themselves filter feeders, and are therefore potential competitors for food resource with the cultured species. Fouling organisms may also reduce the oxygen supply. A study was conducted to measure the impact of foulers on feeding oxygen consumption, and waste excretion. POM, ammonia, phosphate and oxygen concentration were measured in various treatments (cultured species, foulers). This study showed that fouling organisms had significant effect on food uptake, oxygen consumption and waste excretion. The clearance rate, ammonia and phosphate release rate, oxygen consumption rate of the fouled pearl oyster were greater than those of the clean pearl oysters. Other foulers that settled on cages or buoys also contributed much to phytoplankton depletion, oxygen consumption and concentration increase of ammonia and phosphate in water. Therefore, this study showed us that foulers were important competitors in the pearl oyster cultivation of Daya Bay in November 2005.
基金This work is supported by National High-Tech R & D Program of China,(863 Program) (2002AA603014)
文摘Effects of temperatures and salinities on oxygen consumption and ammonia-N excretion rate of clam Meretrix meretrix were studied in laboratory from Oct. 2003 to Jan. 2004. Two schemes were designed in incremented temperature at 10, 15, 20, 25℃ at 31.5 salinity and in incremented salinity at16.0, 21.0, 26.0, 31.5, 36.0, and 41.0 at 20℃, all for 8-10 days. From 10 to 25℃, both respiration and excretion rate were increased. One-way ANOVA analysis demonstrated significant difference (P〈0.01) in physiological parameters in this temperature range except between 15 and 20℃. The highest Q10 thermal coefficient value (12.27) was acquired between 10 and 15℃, and about 1 between 15 and 20℃, indicating M. meretrix could well acclimate to temperature changes in this range. Salinity also had significant effects on respiration and excretion rate (P〈0.05). The highest values of respiration and excretion rate of M. meretrix were recorded at 16.0 salinity (20℃). These two physiological parameters decreased as salinity increased until reached the minimum Q10 value at 31.5 (20℃), then again, these parameters increased with increasing salinity from 31.5 to 41.0. M. meretrix can catabolize body protein to cope with osmotic pressure stress when environmental salinity is away from its optimal range. No significant difference was observed between 26.0 and 36.0 in salinity (P〉0.05), suggesting that a best metabolic salinity range for this species is between 26.0 and 36.0.
基金supported by the National Natural Science Foundation of China under contract Nos.40776074and 30570223
文摘To investigate sulfide detoxification in Urech& unicinctus, oxygen consumption rate and sulfide detoxification products were analyzed during sulfide exposure under controlled laboratory conditions. The results showed that oxygen consumption rates were elevated significantly during 3 h sulfide exposure compared to the control (P〈0.05). The concentration of sulfite in body wall and hindgut of experimental worms increased significantly (P〈0.05) when exposed to 50 μmolL^-1 sulfide, reached a maximum at 24 h and then decreased. Similar result was observed in worms exposed to 150 μmolL^-1 sulfide except that sulfite concentration reached a maximum at 12 h. Contents of thiosulfate in body wall and hindgut of U. unicinctus exposed to sulfide were also significantly higher than that of the control without sulfide exposure. In conclusion, during short-time sulfide exposure U. unicinctus may raise oxygen consumption to detoxify toxic sulfide into sulfite and thiosulfate. Sulfide detoxification was restrained when the exposure time was prolonged or sulfide concentration was increased, which was indicated by decrease of sulfite, the intermediate product of sulfide detoxification.
文摘Effects of reduced salinity on the oxygen consumption rate (OCR) and the ammonia N excretion rate (AER) of scallop Chlamys farreri (3.2 5.9 cm in shell height, 0.147 1.635 g in soft tissue dry weight ) were studied in laboratory from March 21,1997 to April 16, 1997. Under the controlled conditions of reduced salinity from 31.5 to 15.0 and ambient temperature 17℃ and 23℃, the concentrations of dissolved oxygen and ammonia N were determined by the Winkle method and the hypobromite method, respectively. Results showed that with controlled reduced salinity, the mean values of the OCR were 2.17 mg/(g.h) at 17℃, and 2.86 mg/(g.h) at 23℃and that the mean values of the AER were 178.0 μg/(g.h) at 17℃ and 147.0 μg/(g.h) at 23℃. The OCR and the AER decreased with reducing salinity from 31.5 to 15.0 both at 17℃ and 23℃. The effects of reduced salinity on the OCR and the AER of scallop C. farreri could be represented by the allometric equation and the exponential equation, respectively.
基金funded by the Natural Science Foundation ofHeilongjiang Province of China (C201038)the Project of University Innovative Research Team of Heilongjiang Province,China (The Research on Mechanism and Control Technology of Forest Rodents Population Crash)
文摘[Objective]The study aimed to determine oxygen consumption of Erinaceus europaeus at different temperatures to accumulate basic da- ta for the protection and deep discuss of Erinaceus europaeus. [Method]By using the improved close respiration apparatus,the time spent by ani- mals consuming a unit volume of oxygen was recorded by the improved close respiration apparatus to estimate oxygen consumption of Erinaceus europaeus at 20,25 and 30 °C respectively. [Result]When the temperature varied from 20 to 30 °C,the oxygen consumption and oxygen con- sumption rate of Erinaceus europaeus reduced with the increase of external temperature on the whole. The weight of Erinaceus europaeus had no obvious relation with its oxygen consumption and oxygen consumption rate. In addition,the temperature had linear regression relationships with the oxygen consumption and oxygen consumption rate of Erinaceus europaeus,that is,the linear regression equation between oxygen consumption ( x 0 ) ( ml/h) and temperature ( t) was x 0 =658. 81 -14. 98t,and the equation between oxygen consumption rate ( Q 0 ) [L/( kg·h) ]and tempera- ture ( t) was Q 0 =827. 94 -19. 52t. [Conclusion]The metabolic rate of Erinaceus europaeus changed with environmental temperature,and 20 °C was more suitable for its growth,conforming with the fact that it is active just at night.
文摘The effects of water temperature on oxygen consumption rate and ammonia excretion rate of Solenaia oleivora were studied in the laboratory. The results showed that, under controlled conditions and ambient temperatures 15—30℃, the relationship between oxygen consumption rate (O) [mg/h] and dry weight of soft tissue (W) [g] can be represented by an allometric equation O=aW b, while the relationship between ammonia excretion rate (N) [μg/h] and dry weight of soft tissue (W) [g] follows also an allometric equation N=cW b. It is indicated that both metabolic rates are correlated positively with water temperature. High temperature can reduce the level of protein metabolism. The linear regression among oxygen consumption rate (O), temperature (T) and dry weight of soft tissue (W) can be described by the equation O=-0.6513+0.0532T+0.1073W, and for ammonia this relation is N=32.1626-1.0566T+1.3222W, the multiple relation coefficient was 0.9642 and 0.8921, respectively.
文摘of temperature on oxygen consumption rate (OCR) and ammonia-N excretion rate of scallop Chlamys farreri (1.7-6.2 cm in shell height) were studied in laboratory from Dec. 30 1996 to Jan. 28, 1997 . Under the controlled conditions of ambient water temperature 10-31℃ and salinity 32, the concentrations of dissolved oxygen and ammonia-N were determined by the Winkle method and the hypobromite method , respectively . Results showed that the OCR ranged from 1.20 mg/g (DW). h to 5.76mg/g (DW). h. The OCR increased with temperature from 10℃ to 23℃, but at 28℃ the OCR of mature individuals decreased, and that of different size scallops reduced at 31℃. The ammonia-N excretion rate ranged from 113.13 μg NH4-N/g (DW). h to 486.63 μg NH4 -N/g (DW). h,and increased with temperature from 10℃ to 31℃.
基金Supported by the National Key Technology Research and Development Program of China(No.2011BAD13B01)the National Natural Science Foundation of China(General Program)(No.41376165)the China Agriculture Research System CARS-47
文摘Ridgetail white prawn (Exopalaemon carinicauda) are of significant economic importance in China where they are widely cultured. However, there is little information on the basic biology of this species. We evaluated the effect of temperature (16, 19, 22, 25, 28, 31, and 34℃) on the standard metabolic rates (SMRs) of juvenile and adult E. carinicauda in the laboratory under static conditions. The oxygen consumption rate (OCR), ammonia-N excretion rate (AER), and atornic ratio of oxygen consumed to nitrogen consumed (O:N ratio) of juvenile and adult E. carinicauda were significantly influenced by temperature (P〈0.05). Both the OCR and AER of juveniles increased significantly with increasing temperature from 16 to 34℃, but the maximum OCR for adults was at 31℃. Juvenile shrimp exhibited a higher OCR than the adults from 19 to 34℃. There was no significant difference between the AERs of the two life-stages from 16 to 31 ℃ (P〉0.05). The O:N ratio in juveniles was significantly higher than that in the adults over the entire temperature range (P〈0.05). The temperature coefficient (Q_10) of OCR and AER ranged from 5.03 to 0.86 and 6,30 to 0.85 for the adults, respectively, and from 6,09-1.03 and 3.66-1.80 for the juveniles, respectively. The optimal temperature range for growth of the juvenile and adult shrimp was from 28 to 31℃, based on Q_10 and SMR values. Results from the present study may be used to guide pond culture production ofE. carinicauda.