AIM: To investigate the therapeutic effects of resveratrol (RESV) as a free radical scavenger on experimental severe acute pancreatitis (SAP). METHODS: Seventy-two male Sprague-Dawley rats were divided randomly ...AIM: To investigate the therapeutic effects of resveratrol (RESV) as a free radical scavenger on experimental severe acute pancreatitis (SAP). METHODS: Seventy-two male Sprague-Dawley rats were divided randomly into sham operation group, SAP group, and resveratrol-treated group. Pancreatitis was induced by intraductal administration of 0.1 mL/kg 4% sodium taurocholate. RESV was given intravenously at a dose of 20 mg/kg body weight. All animals were killed at 3, 6, 12 h after induction of the model. Serum amylase, pancreatic superoxide dismutase (SOD), malondialdehyde (MDA), and myeloperoxidase (MPO) were determined. Pathologic changes of the pancreas were observed under optical microscope. RESULTS: The serum amylase, pancreatic MPO and the score of pathologic damage increased after the induction of pancreatitis, early (3, 6 h) SAP samples were characterized by decreased pancreatic SOD and increased pancreatic MDA. Resveratrol exhibited a protective effect against lipid peroxidation in cell membrane caused by oxygen free radicals in the early stage of SAP. This attenuation of the redox state impairment reduced cellular oxidative damage, as reflected by lower serum amylase, less severe pancreatic lesions, normal pancreatic MDA levels, as well as diminished neutrophil infiltration in pancreas. CONCLUSION: RESV may exert its therapeutic effect on SAP by lowering pancreatic oxidative free radicals and reducing pancreatic tissue infiltration of neutrophils.展开更多
AIM: Microcirculatory dysfunction and free oxygen radicals are important factors in the pathogenesis of severe acute pancreatitis. Additional oxygen delivery might enhance lipid peroxidation but may also improve panc...AIM: Microcirculatory dysfunction and free oxygen radicals are important factors in the pathogenesis of severe acute pancreatitis. Additional oxygen delivery might enhance lipid peroxidation but may also improve pancreatic microcirculation. This study assesses the effect of free cellular bovine hemoglobin on the formation of oxygen radicals and microcirculation in a rodent model of severe acute pancreatitis. METHODS: Fifteen minutes after induction of acute pancreatitis Wistar rats received either 0.8 mL bovine hemoglobin (HBOC-200), hydroxyethyl starch (HES) or 2.4 mL of normal saline to ensure normovolemic substitution. After 6 h of examination the pancreas was excised and rapidly processed for indirect measurement of lipid peroxidation products malondialdehyde (MDA) and reduced glutathione (GSH) in pancreatic tissue. RESULTS: The single application of HBOC-200 improved pancreatic microcirculation and reduced histopathological tissue damage significantly. Tissue concentration of MDA did not differ between the groups. Also no differences in GSH levels were detected.CONCLUSION: Though the single application of HBOC-200 and HES improve pancreatic microcirculation, no differences in lipid peroxidation products were detected. The beneficial effect of additional oxygen supply (HBOC-200) does not lead to enhanced lipid peroxidation.展开更多
Objective: To study the effects of ulinastatin combined with surgery on nerve injury, oxygen free radicals and inflammatory factors production of patients with hypertensive intracerebral hemorrhage. Methods: The patie...Objective: To study the effects of ulinastatin combined with surgery on nerve injury, oxygen free radicals and inflammatory factors production of patients with hypertensive intracerebral hemorrhage. Methods: The patients with hypertensive intracerebral hemorrhage received surgical treatment in our hospital during February 2015 - December 2017 were selected and divided into two groups by random number table. The observation group received ulinastatin combined surgery and the control group received conventional medicine combined with surgery. Before treatment and 1 weeks after treatment, serum levels of nerve damage markers, neurotrophic indexes, oxygen free radicals production indexes and inflammatory cytokines were measured. Results: After treatment, the contents of NSE, VILIP-1, GFAP, S100B, MDA, AOPP, 8-OHdG, NO, ET-1, TNF-α, sICAM-1, sVCAM-1, sICAM-1 of two groups were all decreased, the contents of BDNF, NGF, VEGF, IGF-I were all increased and the decreasing trend of NSE, VILIP-1, GFAP, S100B, MDA, AOPP, 8-OHdG, NO, ET-1, TNF-α, sICAM-1, sVCAM-1, sICAM-1 of observation group was more obvious than that of the control group, the increasing trend of BDNF, NGF, VEGF, IGF-I content was more obvious than that of the control group. Conclusion: The use of ulinastatin combined with surgery in the treatment of hypertensive intracerebral hemorrhage can significantly reduce the damage of nerve function and reduce the production of oxygen free radicals and inflammatory factors.展开更多
A long life election spin resonance (ESR) signal at g=2.0006 was observed in the normal lens epithelium and cortical fibers. During ultraviolet (UV) exposure, a new ESR signal at g = 2.0060 was found in the lens epith...A long life election spin resonance (ESR) signal at g=2.0006 was observed in the normal lens epithelium and cortical fibers. During ultraviolet (UV) exposure, a new ESR signal at g = 2.0060 was found in the lens epithelium. But this specific signal was not detected in the lens cortical fibers. This suggested that lens epithelial cells were more susceptible to the free radical formation which was induced by UV light. By means of ESR spin probe oxymetry, the oxygen uptake of lens epithelial cells was meas...展开更多
The correlation of oxygen free radical items between blood and kidney of 5/6 nephrectomy-induced chronic renal failure rats was studied. It was found that oxygen free radicals played an important role in the impairmen...The correlation of oxygen free radical items between blood and kidney of 5/6 nephrectomy-induced chronic renal failure rats was studied. It was found that oxygen free radicals played an important role in the impairment of remnant kidney,and antioxidant vitamin E could protect remnant kidney from the impairment caused by oxygen free radicals,and that,both in normal and chronic renal failure conditions,plasma lipid peroxides and vitamin E concentration and superoxide dismutase activity of red blood cells correlated very well with corresponding items of remnant kidney.These results suggested that blood oxygen free radical items could reflect renal oxygen free radical metabolic status in chronic renal failure patients.展开更多
[ Objective] This study was to investigate the effect of selenium(Se) on immune function and oxygen free radicals in erythrocyte of pig. [ Method] Fifteen weanling Landrace piglets as experimental animals were divid...[ Objective] This study was to investigate the effect of selenium(Se) on immune function and oxygen free radicals in erythrocyte of pig. [ Method] Fifteen weanling Landrace piglets as experimental animals were divided into three groups, two testing groups and one control group. For each group, Se content in whole blood, immune function of erythrocytes, activity of whole blood glutathione peroxidase(GSH-Px), activity of blood plasma superoxide dismutase(SOD), and blood plasma malondialdehyde(MDA) content were determined. [ Result] Compared with the control group, Se content in whole blood and blood plasma MDA content increased remarkably, while whole blood GSH-Px activity and blood plasma SOD activity decreased; RBC-C1 RR assumed a rise-fall trend, and RBC-ICR showed no obvious change. [Condusion] Se poisoning can reduce the activity of antioxidant enzymes, disturb the balance of oxygen free radicals metabolism, thereby inducing erythrooyte immune function in piglets.展开更多
The protective effect of captopril on ischemic myocardium was studied in 40 patients with congenital heart disease accompanied with pulmonary hypertention.Twenty of these patients received captopril 50 mg b.i.d.for th...The protective effect of captopril on ischemic myocardium was studied in 40 patients with congenital heart disease accompanied with pulmonary hypertention.Twenty of these patients received captopril 50 mg b.i.d.for three months preoperatively (Group A).The other 20 patients without pretreatment with captopril were used as controls (Group B).In Group A,the cardiac output increased and the pulmonary pressure decreased significantly in the first 24 hours postoperatively,indicating a high cardiac output with a low vascular resistance.The differences or CPK,CPKMB,LDH release between Group A and Group B were highly significant or siguificant from the end of operation to 24 hours postoperatively. Myocardial protection in the reduction of the release of CPK, CPK-MB, LDH might be attributed to action on scavenging oxygen free radicals.展开更多
Objective: To investigate the effect of ultraviolet blood irradiation and oxygenation (UBIO) on the metabolism of oxygen free radicals in rabbits with acute soman intoxication. Methods: One hundred rabbits were random...Objective: To investigate the effect of ultraviolet blood irradiation and oxygenation (UBIO) on the metabolism of oxygen free radicals in rabbits with acute soman intoxication. Methods: One hundred rabbits were randomly divided into 5 groups: normal control group, intoxication group, routine therapy group. UBIO therapy group and combined therapy group. After 14 d, the concentration of malondiadehyde(MDA) and activity of superoxide dismutase(SOD), glutathionperoxidase(GSH-Px), catalase (CAT) and total antioxidative capacity (T-AOC) in serum were determined respectively. Results: Compared with the normal control group, the concentration of MDA and activity of CAT in the intoxication group were significantly higher (P < 0. 05). but SOD. GSH-Px activity and T-AOC were significantly lower (P<0. 05). After UBIO or combined therapy, serum MDA level was significantly lower in comparison with intoxication group (P<0. 05). but the activity of SOD. GSH-Px, CAT and T-AOC were higher than intoxication group(P<0. 05). Conclusion: There is an obvious oxygen free radical injury in rabbits with a-cute soman intoxication. UBIO can improve the antioxidation ability of rabbits and may be applied to treat acute soman intoxication as adjunctive therapy.展开更多
Objective: To observe the effect of acupuncture on blood oxygen free radical (OFR) and nitric oxide (NO) levels in the treatment of apoplectic sequelae. Methods: A total of 61 cases of apoplectic patients were subject...Objective: To observe the effect of acupuncture on blood oxygen free radical (OFR) and nitric oxide (NO) levels in the treatment of apoplectic sequelae. Methods: A total of 61 cases of apoplectic patients were subjected into this study and randomly divided into "JIN San Zhen" group (n=30) and control group (n=31). Blood lipid peroxidase (LPO), superoxide dismutase (SOD), glutathione peroxidase(GSH Px) and nitric oxide (NO) contents before and after acupuncture treatment were determined with radioimmunoassay. In both groups, acupuncture was given once daily, six times a week, with 4 weeks being a therapeutic course and with the interval between two weeks being a week, 3 courses all together. In "JIN San Zhen" group, acupoints of "JIN San Zhen" were used predominately, while in control group, scalp point Motor Sensory Area (MS 8) was used as the main point. Results: Self comparison showed that after 3 courses of treatment, in both groups, LPO and NO levels decreased significantly (P<0.05-0.01), SOD and GST Px values increased considerably (P<0.05-0.01). Comparison between two groups indicated that the effects of "JIN San Zhen" group are significantly superior to those of control group in raising blood SOD and GST Px levels (P<0.05-0.01) and in lowering blood NO content (P<0.01). Analysis on the correlation between the restoration of neural function and the changes of LPO, SOD and GST Px levels suggested that the effect of acupuncture in improving neural function may be related to changes of the aforementioned indexes. Conclusion: Acupuncture therapy can significantly lower blood LPO and NO levels and evidently raise blood SOD and GST Px levels in stroke patients.展开更多
Objective: To study the effect of dexmedetomidine combined with ulinastatin on RAAS system, coagulation indexes and oxygen free radicals after acute aortic dissection surgery. Methods: A total of 48 patients with aort...Objective: To study the effect of dexmedetomidine combined with ulinastatin on RAAS system, coagulation indexes and oxygen free radicals after acute aortic dissection surgery. Methods: A total of 48 patients with aortic dissection who accepted endovascular graft exclusion treatment in the hospital between May 2014 and February 2017 were selected and randomly divided into two groups, group A received dexmedetomidine, ulinastatin combined with general anesthesia, and group B received general anesthesia. The RAAS hormones, coagulation indexes and oxygen free radical indexes were measured 1 d before surgery, during operation and 24 h after surgery. Results: During operation and after operation, serum REN, AT-II, ALD, FIB, DD, MDA and MPO contents as well as APTT and TT levels of both groups of patients were significantly higher than those before operation while TAOC and TSOD contents were significantly lower than those before operation, and serum REN, AT-II, ALD, FIB, DD, MDA and MPO contents as well as APTT and TT levels of group A were significantly lower than those of group B while TAOC and TSOD contents were significantly higher than those of group B. Conclusion: Dexmedetomidine combined with ulinastatin for acute aortic dissection can inhibit RAAS system and oxygen free radical generation and improve coagulation function.展开更多
Objective: To study the effect of early rehabilitation training combined with edaravone on oxygen free radical generation and nerve injury in patients with cerebral hemorrhage. Methods: A total of 56 patients with acu...Objective: To study the effect of early rehabilitation training combined with edaravone on oxygen free radical generation and nerve injury in patients with cerebral hemorrhage. Methods: A total of 56 patients with acute cerebral hemorrhage who were treated in Zigong Third People's Hospital between July 2014 and March 2017 were selected and randomly divided into early rehabilitation group and routine rehabilitation group, the early rehabilitation group began the rehabilitation training 2 d after cerebral hemorrhage condition was stabilized, and routine rehabilitation group began the rehabilitation training 14 d after cerebral hemorrhage. Serum contents of oxygen free radicals, nerve injury markers and neurotrophic molecules were detected 28 d and 56 d after cerebral hemorrhage. Results: 28 d and 56 d after cerebral hemorrhage, serum MDA, AOPP, 8-OHdG, GFAP, NSE, Tf, Ft and S100B levels of early rehabilitation group were significantly lower than those of routine rehabilitation group while BDNF, NGF, NTF-α and IGF-I levels were significantly higher than those of routine rehabilitation group. Conclusion: Early rehabilitation training combined with edaravone for cerebral hemorrhage can inhibit the oxygen free radical generation, reduce the degree of nerve injury and improve the neurotrophic state.展开更多
Objective: To study the correlation of electrophysiological characteristics Tp-ec and Tp-e/QT with myocardial injury and oxygen free radical generation in patients with acute myocardial infarction. Methods: Patients w...Objective: To study the correlation of electrophysiological characteristics Tp-ec and Tp-e/QT with myocardial injury and oxygen free radical generation in patients with acute myocardial infarction. Methods: Patients who were diagnosed with acute myocardial infarction in our hospital between March 2014 and March 2017 were selected as the AMI group, and healthy subjects who received physical examination were selected as the control group, electrocardiography was done to determine Tp-ec and Tp-e/QT, serum was collected to determine the levels of myocardial injury markers, oxygen free radical generation indexes and apoptosis indexes, and peripheral blood was collected to determine the expression of oxygen free radical generation indexes. Results: Tp-ec and Tp-e/QT of AMI group were significantly higher than those of control group, and serum CK-MB, cTnI, H-FABP, MDA, sTWEAK, sFas and sTRAIL contents as well as peripheral blood Nrf-2, NRE and HO-1 mRNA expression were significantly higher than those of control group and positively correlated with Tp-ec and Tp-e/QT. Conclusion: Electrophysiological characteristics Tp-ec and Tp-e/QT increase, and the transseptal dispersion of repolarization increases in patients with acute myocardial infarction, and they are closely related to myocardial injury and oxygen free radical generation.展开更多
Excitatory amino acid toxicity and free radical damage play important roles in amyotrophic lateral sclerosis. Granulocyte colony-stimulating factor (G-CSF) protects nerve cells exposed to high-concentrations of glut...Excitatory amino acid toxicity and free radical damage play important roles in amyotrophic lateral sclerosis. Granulocyte colony-stimulating factor (G-CSF) protects nerve cells exposed to high-concentrations of glutamic acid, suggesting positive effects in the treatment of amyotrophic lateral sclerosis. The present study induced in vitro motor neuron injury using glutamic acid excitotoxicity, and the biochemical effects of G-CSF on glutamic acid concentration were determined. In addition, the effects of G-CSF on superoxide dismutase, glutathione peroxidase activity in motor neurons, and malondialdehyde and nitric oxide contents were analyzed. Immunohistochemistry was performed to measure neuronal survival. Results revealed that G-CSF significantly suppressed free radical activity, inhibited excitotoxicity, and reduced apoptosis and loss of motor neurons in the anterior horn of the spinal cord.展开更多
The role of reactive oxygen species(ROS)in ischemic and reperfusion(I/R)injury of the heart has been discussed for more than 40 years.It has been demonstrated that reperfusion triggers a multiple increase in free radi...The role of reactive oxygen species(ROS)in ischemic and reperfusion(I/R)injury of the heart has been discussed for more than 40 years.It has been demonstrated that reperfusion triggers a multiple increase in free radical generation in the isolated heart.Antioxidants were found to have the ability to mitigate I/R injury of the heart.However,it is unclear whether their cardioprotective effect truly depends on the decrease of ROS levels in myocardial tissues.Since high doses and high concentrations of antioxidants were experimentally used,it is highly likely that the cardioprotective effect of antioxidants depends on their interaction not only with free radicals but also with other molecules.It has been demonstrated that the antioxidant N-2-mercaptopropionyl glycine or NDPH oxidase knockout abolished the cardioprotective effect of ischemic preconditioning.Consequently,there is evidence that ROS protect the heart against the I/R injury.展开更多
We conducted the novel synthesis of middle molecular weight heparinyl amino acid derivatives (MHADs) to reduce the adverse effect of heparin (HE) based on its anticoagulant activity. Subsequently, we investigated the ...We conducted the novel synthesis of middle molecular weight heparinyl amino acid derivatives (MHADs) to reduce the adverse effect of heparin (HE) based on its anticoagulant activity. Subsequently, we investigated the radical scavenging effects of 12 kinds of MHAD on cultured human umbilical vein endothelial cells (HUV-ECs) damaged by oxygen free radicals using xanthine and xanthine oxidase in vitro. As a result, middle molecular weight heparinyl phenylalanine, middle molecular weight heparinyl leucine, and middle molecular weight heparinyl tyrosine showed significant protective effects on HUV-ECs. In conclusion, these three HE derivatives might be candidates for therapeutic agents to treat diseases attributed to peroxidation.展开更多
文摘AIM: To investigate the therapeutic effects of resveratrol (RESV) as a free radical scavenger on experimental severe acute pancreatitis (SAP). METHODS: Seventy-two male Sprague-Dawley rats were divided randomly into sham operation group, SAP group, and resveratrol-treated group. Pancreatitis was induced by intraductal administration of 0.1 mL/kg 4% sodium taurocholate. RESV was given intravenously at a dose of 20 mg/kg body weight. All animals were killed at 3, 6, 12 h after induction of the model. Serum amylase, pancreatic superoxide dismutase (SOD), malondialdehyde (MDA), and myeloperoxidase (MPO) were determined. Pathologic changes of the pancreas were observed under optical microscope. RESULTS: The serum amylase, pancreatic MPO and the score of pathologic damage increased after the induction of pancreatitis, early (3, 6 h) SAP samples were characterized by decreased pancreatic SOD and increased pancreatic MDA. Resveratrol exhibited a protective effect against lipid peroxidation in cell membrane caused by oxygen free radicals in the early stage of SAP. This attenuation of the redox state impairment reduced cellular oxidative damage, as reflected by lower serum amylase, less severe pancreatic lesions, normal pancreatic MDA levels, as well as diminished neutrophil infiltration in pancreas. CONCLUSION: RESV may exert its therapeutic effect on SAP by lowering pancreatic oxidative free radicals and reducing pancreatic tissue infiltration of neutrophils.
文摘AIM: Microcirculatory dysfunction and free oxygen radicals are important factors in the pathogenesis of severe acute pancreatitis. Additional oxygen delivery might enhance lipid peroxidation but may also improve pancreatic microcirculation. This study assesses the effect of free cellular bovine hemoglobin on the formation of oxygen radicals and microcirculation in a rodent model of severe acute pancreatitis. METHODS: Fifteen minutes after induction of acute pancreatitis Wistar rats received either 0.8 mL bovine hemoglobin (HBOC-200), hydroxyethyl starch (HES) or 2.4 mL of normal saline to ensure normovolemic substitution. After 6 h of examination the pancreas was excised and rapidly processed for indirect measurement of lipid peroxidation products malondialdehyde (MDA) and reduced glutathione (GSH) in pancreatic tissue. RESULTS: The single application of HBOC-200 improved pancreatic microcirculation and reduced histopathological tissue damage significantly. Tissue concentration of MDA did not differ between the groups. Also no differences in GSH levels were detected.CONCLUSION: Though the single application of HBOC-200 and HES improve pancreatic microcirculation, no differences in lipid peroxidation products were detected. The beneficial effect of additional oxygen supply (HBOC-200) does not lead to enhanced lipid peroxidation.
文摘Objective: To study the effects of ulinastatin combined with surgery on nerve injury, oxygen free radicals and inflammatory factors production of patients with hypertensive intracerebral hemorrhage. Methods: The patients with hypertensive intracerebral hemorrhage received surgical treatment in our hospital during February 2015 - December 2017 were selected and divided into two groups by random number table. The observation group received ulinastatin combined surgery and the control group received conventional medicine combined with surgery. Before treatment and 1 weeks after treatment, serum levels of nerve damage markers, neurotrophic indexes, oxygen free radicals production indexes and inflammatory cytokines were measured. Results: After treatment, the contents of NSE, VILIP-1, GFAP, S100B, MDA, AOPP, 8-OHdG, NO, ET-1, TNF-α, sICAM-1, sVCAM-1, sICAM-1 of two groups were all decreased, the contents of BDNF, NGF, VEGF, IGF-I were all increased and the decreasing trend of NSE, VILIP-1, GFAP, S100B, MDA, AOPP, 8-OHdG, NO, ET-1, TNF-α, sICAM-1, sVCAM-1, sICAM-1 of observation group was more obvious than that of the control group, the increasing trend of BDNF, NGF, VEGF, IGF-I content was more obvious than that of the control group. Conclusion: The use of ulinastatin combined with surgery in the treatment of hypertensive intracerebral hemorrhage can significantly reduce the damage of nerve function and reduce the production of oxygen free radicals and inflammatory factors.
基金The work was sponsored by National Natural Science Foundation of China (NSFC)
文摘A long life election spin resonance (ESR) signal at g=2.0006 was observed in the normal lens epithelium and cortical fibers. During ultraviolet (UV) exposure, a new ESR signal at g = 2.0060 was found in the lens epithelium. But this specific signal was not detected in the lens cortical fibers. This suggested that lens epithelial cells were more susceptible to the free radical formation which was induced by UV light. By means of ESR spin probe oxymetry, the oxygen uptake of lens epithelial cells was meas...
文摘The correlation of oxygen free radical items between blood and kidney of 5/6 nephrectomy-induced chronic renal failure rats was studied. It was found that oxygen free radicals played an important role in the impairment of remnant kidney,and antioxidant vitamin E could protect remnant kidney from the impairment caused by oxygen free radicals,and that,both in normal and chronic renal failure conditions,plasma lipid peroxides and vitamin E concentration and superoxide dismutase activity of red blood cells correlated very well with corresponding items of remnant kidney.These results suggested that blood oxygen free radical items could reflect renal oxygen free radical metabolic status in chronic renal failure patients.
文摘[ Objective] This study was to investigate the effect of selenium(Se) on immune function and oxygen free radicals in erythrocyte of pig. [ Method] Fifteen weanling Landrace piglets as experimental animals were divided into three groups, two testing groups and one control group. For each group, Se content in whole blood, immune function of erythrocytes, activity of whole blood glutathione peroxidase(GSH-Px), activity of blood plasma superoxide dismutase(SOD), and blood plasma malondialdehyde(MDA) content were determined. [ Result] Compared with the control group, Se content in whole blood and blood plasma MDA content increased remarkably, while whole blood GSH-Px activity and blood plasma SOD activity decreased; RBC-C1 RR assumed a rise-fall trend, and RBC-ICR showed no obvious change. [Condusion] Se poisoning can reduce the activity of antioxidant enzymes, disturb the balance of oxygen free radicals metabolism, thereby inducing erythrooyte immune function in piglets.
文摘The protective effect of captopril on ischemic myocardium was studied in 40 patients with congenital heart disease accompanied with pulmonary hypertention.Twenty of these patients received captopril 50 mg b.i.d.for three months preoperatively (Group A).The other 20 patients without pretreatment with captopril were used as controls (Group B).In Group A,the cardiac output increased and the pulmonary pressure decreased significantly in the first 24 hours postoperatively,indicating a high cardiac output with a low vascular resistance.The differences or CPK,CPKMB,LDH release between Group A and Group B were highly significant or siguificant from the end of operation to 24 hours postoperatively. Myocardial protection in the reduction of the release of CPK, CPK-MB, LDH might be attributed to action on scavenging oxygen free radicals.
文摘Objective: To investigate the effect of ultraviolet blood irradiation and oxygenation (UBIO) on the metabolism of oxygen free radicals in rabbits with acute soman intoxication. Methods: One hundred rabbits were randomly divided into 5 groups: normal control group, intoxication group, routine therapy group. UBIO therapy group and combined therapy group. After 14 d, the concentration of malondiadehyde(MDA) and activity of superoxide dismutase(SOD), glutathionperoxidase(GSH-Px), catalase (CAT) and total antioxidative capacity (T-AOC) in serum were determined respectively. Results: Compared with the normal control group, the concentration of MDA and activity of CAT in the intoxication group were significantly higher (P < 0. 05). but SOD. GSH-Px activity and T-AOC were significantly lower (P<0. 05). After UBIO or combined therapy, serum MDA level was significantly lower in comparison with intoxication group (P<0. 05). but the activity of SOD. GSH-Px, CAT and T-AOC were higher than intoxication group(P<0. 05). Conclusion: There is an obvious oxygen free radical injury in rabbits with a-cute soman intoxication. UBIO can improve the antioxidation ability of rabbits and may be applied to treat acute soman intoxication as adjunctive therapy.
文摘Objective: To observe the effect of acupuncture on blood oxygen free radical (OFR) and nitric oxide (NO) levels in the treatment of apoplectic sequelae. Methods: A total of 61 cases of apoplectic patients were subjected into this study and randomly divided into "JIN San Zhen" group (n=30) and control group (n=31). Blood lipid peroxidase (LPO), superoxide dismutase (SOD), glutathione peroxidase(GSH Px) and nitric oxide (NO) contents before and after acupuncture treatment were determined with radioimmunoassay. In both groups, acupuncture was given once daily, six times a week, with 4 weeks being a therapeutic course and with the interval between two weeks being a week, 3 courses all together. In "JIN San Zhen" group, acupoints of "JIN San Zhen" were used predominately, while in control group, scalp point Motor Sensory Area (MS 8) was used as the main point. Results: Self comparison showed that after 3 courses of treatment, in both groups, LPO and NO levels decreased significantly (P<0.05-0.01), SOD and GST Px values increased considerably (P<0.05-0.01). Comparison between two groups indicated that the effects of "JIN San Zhen" group are significantly superior to those of control group in raising blood SOD and GST Px levels (P<0.05-0.01) and in lowering blood NO content (P<0.01). Analysis on the correlation between the restoration of neural function and the changes of LPO, SOD and GST Px levels suggested that the effect of acupuncture in improving neural function may be related to changes of the aforementioned indexes. Conclusion: Acupuncture therapy can significantly lower blood LPO and NO levels and evidently raise blood SOD and GST Px levels in stroke patients.
文摘Objective: To study the effect of dexmedetomidine combined with ulinastatin on RAAS system, coagulation indexes and oxygen free radicals after acute aortic dissection surgery. Methods: A total of 48 patients with aortic dissection who accepted endovascular graft exclusion treatment in the hospital between May 2014 and February 2017 were selected and randomly divided into two groups, group A received dexmedetomidine, ulinastatin combined with general anesthesia, and group B received general anesthesia. The RAAS hormones, coagulation indexes and oxygen free radical indexes were measured 1 d before surgery, during operation and 24 h after surgery. Results: During operation and after operation, serum REN, AT-II, ALD, FIB, DD, MDA and MPO contents as well as APTT and TT levels of both groups of patients were significantly higher than those before operation while TAOC and TSOD contents were significantly lower than those before operation, and serum REN, AT-II, ALD, FIB, DD, MDA and MPO contents as well as APTT and TT levels of group A were significantly lower than those of group B while TAOC and TSOD contents were significantly higher than those of group B. Conclusion: Dexmedetomidine combined with ulinastatin for acute aortic dissection can inhibit RAAS system and oxygen free radical generation and improve coagulation function.
文摘Objective: To study the effect of early rehabilitation training combined with edaravone on oxygen free radical generation and nerve injury in patients with cerebral hemorrhage. Methods: A total of 56 patients with acute cerebral hemorrhage who were treated in Zigong Third People's Hospital between July 2014 and March 2017 were selected and randomly divided into early rehabilitation group and routine rehabilitation group, the early rehabilitation group began the rehabilitation training 2 d after cerebral hemorrhage condition was stabilized, and routine rehabilitation group began the rehabilitation training 14 d after cerebral hemorrhage. Serum contents of oxygen free radicals, nerve injury markers and neurotrophic molecules were detected 28 d and 56 d after cerebral hemorrhage. Results: 28 d and 56 d after cerebral hemorrhage, serum MDA, AOPP, 8-OHdG, GFAP, NSE, Tf, Ft and S100B levels of early rehabilitation group were significantly lower than those of routine rehabilitation group while BDNF, NGF, NTF-α and IGF-I levels were significantly higher than those of routine rehabilitation group. Conclusion: Early rehabilitation training combined with edaravone for cerebral hemorrhage can inhibit the oxygen free radical generation, reduce the degree of nerve injury and improve the neurotrophic state.
文摘Objective: To study the correlation of electrophysiological characteristics Tp-ec and Tp-e/QT with myocardial injury and oxygen free radical generation in patients with acute myocardial infarction. Methods: Patients who were diagnosed with acute myocardial infarction in our hospital between March 2014 and March 2017 were selected as the AMI group, and healthy subjects who received physical examination were selected as the control group, electrocardiography was done to determine Tp-ec and Tp-e/QT, serum was collected to determine the levels of myocardial injury markers, oxygen free radical generation indexes and apoptosis indexes, and peripheral blood was collected to determine the expression of oxygen free radical generation indexes. Results: Tp-ec and Tp-e/QT of AMI group were significantly higher than those of control group, and serum CK-MB, cTnI, H-FABP, MDA, sTWEAK, sFas and sTRAIL contents as well as peripheral blood Nrf-2, NRE and HO-1 mRNA expression were significantly higher than those of control group and positively correlated with Tp-ec and Tp-e/QT. Conclusion: Electrophysiological characteristics Tp-ec and Tp-e/QT increase, and the transseptal dispersion of repolarization increases in patients with acute myocardial infarction, and they are closely related to myocardial injury and oxygen free radical generation.
文摘Excitatory amino acid toxicity and free radical damage play important roles in amyotrophic lateral sclerosis. Granulocyte colony-stimulating factor (G-CSF) protects nerve cells exposed to high-concentrations of glutamic acid, suggesting positive effects in the treatment of amyotrophic lateral sclerosis. The present study induced in vitro motor neuron injury using glutamic acid excitotoxicity, and the biochemical effects of G-CSF on glutamic acid concentration were determined. In addition, the effects of G-CSF on superoxide dismutase, glutathione peroxidase activity in motor neurons, and malondialdehyde and nitric oxide contents were analyzed. Immunohistochemistry was performed to measure neuronal survival. Results revealed that G-CSF significantly suppressed free radical activity, inhibited excitotoxicity, and reduced apoptosis and loss of motor neurons in the anterior horn of the spinal cord.
文摘The role of reactive oxygen species(ROS)in ischemic and reperfusion(I/R)injury of the heart has been discussed for more than 40 years.It has been demonstrated that reperfusion triggers a multiple increase in free radical generation in the isolated heart.Antioxidants were found to have the ability to mitigate I/R injury of the heart.However,it is unclear whether their cardioprotective effect truly depends on the decrease of ROS levels in myocardial tissues.Since high doses and high concentrations of antioxidants were experimentally used,it is highly likely that the cardioprotective effect of antioxidants depends on their interaction not only with free radicals but also with other molecules.It has been demonstrated that the antioxidant N-2-mercaptopropionyl glycine or NDPH oxidase knockout abolished the cardioprotective effect of ischemic preconditioning.Consequently,there is evidence that ROS protect the heart against the I/R injury.
文摘We conducted the novel synthesis of middle molecular weight heparinyl amino acid derivatives (MHADs) to reduce the adverse effect of heparin (HE) based on its anticoagulant activity. Subsequently, we investigated the radical scavenging effects of 12 kinds of MHAD on cultured human umbilical vein endothelial cells (HUV-ECs) damaged by oxygen free radicals using xanthine and xanthine oxidase in vitro. As a result, middle molecular weight heparinyl phenylalanine, middle molecular weight heparinyl leucine, and middle molecular weight heparinyl tyrosine showed significant protective effects on HUV-ECs. In conclusion, these three HE derivatives might be candidates for therapeutic agents to treat diseases attributed to peroxidation.