[Objectives] To study the rheology and release kinetics of Chuanqi ophthalmic microemulsion in situ gel,so as to provide references for its future study and development. [Methods]The fluid properties and linear viscoe...[Objectives] To study the rheology and release kinetics of Chuanqi ophthalmic microemulsion in situ gel,so as to provide references for its future study and development. [Methods]The fluid properties and linear viscoelastic regions of this preparation were investigated by MCR 102 rheometer. The release kinetics of Chuanqi ophthalmic microemulsion in situ gel was evaluated by modified Franz diffusion cell method,the ligustrazine and ligustilide were selected as the indictors,and semi-permeable membrane was used as a barrier,sampling time point was 0. 5,1,2,4,6,and 8 h respectively. [Results]Chuanqi ophthalmic microemulsion in situ gel was a pseudoplastic fluid and it had a linear viscoelastic region. Taking the shear stress as the indicator,the linear viscoelastic region was 0-302. 74 Pa; taking the strain as the indicator,the linear viscoelastic region was 0-7. 45%. At the critical point,the storage modulus( G') = the loss modulus( G″) =2 976. 60 Pa,critical shear stress was 302. 74 Pa and critical strain was 7. 45%. The average cumulative release of ligustrazine of 6 samples within 8 h was 33. 71 μg,the average cumulative release rate reached 90. 08%,and the release kinetics followed Higuchi equation. The average cumulative release of ligustilide of 6 samples within 8 h was 68. 46 μg,the average cumulative release rate reached 84. 32%,and the release kinetics followed the zero-order kinetics equation. [Conclusions] Chuanqi ophthalmic microemulsion in situ gel has excellent viscoelasticity and its strain is reversible in a certain range. The release kinetics of ligustrazine is the result from synergistic effect of its physicochemical properties and matrix skeleton,while the release kinetics of ligustilide is mainly affected by its physicochemical properties.展开更多
目的观察温度敏感型富血小板血浆凝胶对兔角膜碱烧伤组织的修复效果,探讨角膜损伤修复的有效治疗方法。方法将20只白兔制作兔角膜碱烧伤模型后按随机数字表法分为A、B、C、D组,每组5只,分别采用生理盐水、自体富血小板血浆、温度敏感型...目的观察温度敏感型富血小板血浆凝胶对兔角膜碱烧伤组织的修复效果,探讨角膜损伤修复的有效治疗方法。方法将20只白兔制作兔角膜碱烧伤模型后按随机数字表法分为A、B、C、D组,每组5只,分别采用生理盐水、自体富血小板血浆、温度敏感型富血小板血浆凝胶和凝胶空白基质点眼。于治疗前及治疗后3、5、7、10、14 d采用Ando和Trousdale标准对角膜炎症及溃疡评分,HE染色观察角膜组织病理变化。结果治疗14 d后A组与D组角膜炎症及溃疡评分差异均无统计学意义(P>0.05);B、C组角膜炎症及溃疡评分较A、D组显著降低(P<0.01),且C组角膜炎症及溃疡评分较B组显著降低(P<0.01)。病理所见给药后14 d A组和D组角膜处于炎症和溃疡期,B组角膜处于修复中期,C组角膜处于修复后期。结论温度敏感型富血小板血浆凝胶对兔角膜碱烧伤组织的修复效果良好,是一种有效的眼表修复制剂。展开更多
基金Supported by Project of National Natural Science Foundation(81373977)
文摘[Objectives] To study the rheology and release kinetics of Chuanqi ophthalmic microemulsion in situ gel,so as to provide references for its future study and development. [Methods]The fluid properties and linear viscoelastic regions of this preparation were investigated by MCR 102 rheometer. The release kinetics of Chuanqi ophthalmic microemulsion in situ gel was evaluated by modified Franz diffusion cell method,the ligustrazine and ligustilide were selected as the indictors,and semi-permeable membrane was used as a barrier,sampling time point was 0. 5,1,2,4,6,and 8 h respectively. [Results]Chuanqi ophthalmic microemulsion in situ gel was a pseudoplastic fluid and it had a linear viscoelastic region. Taking the shear stress as the indicator,the linear viscoelastic region was 0-302. 74 Pa; taking the strain as the indicator,the linear viscoelastic region was 0-7. 45%. At the critical point,the storage modulus( G') = the loss modulus( G″) =2 976. 60 Pa,critical shear stress was 302. 74 Pa and critical strain was 7. 45%. The average cumulative release of ligustrazine of 6 samples within 8 h was 33. 71 μg,the average cumulative release rate reached 90. 08%,and the release kinetics followed Higuchi equation. The average cumulative release of ligustilide of 6 samples within 8 h was 68. 46 μg,the average cumulative release rate reached 84. 32%,and the release kinetics followed the zero-order kinetics equation. [Conclusions] Chuanqi ophthalmic microemulsion in situ gel has excellent viscoelasticity and its strain is reversible in a certain range. The release kinetics of ligustrazine is the result from synergistic effect of its physicochemical properties and matrix skeleton,while the release kinetics of ligustilide is mainly affected by its physicochemical properties.
文摘目的观察温度敏感型富血小板血浆凝胶对兔角膜碱烧伤组织的修复效果,探讨角膜损伤修复的有效治疗方法。方法将20只白兔制作兔角膜碱烧伤模型后按随机数字表法分为A、B、C、D组,每组5只,分别采用生理盐水、自体富血小板血浆、温度敏感型富血小板血浆凝胶和凝胶空白基质点眼。于治疗前及治疗后3、5、7、10、14 d采用Ando和Trousdale标准对角膜炎症及溃疡评分,HE染色观察角膜组织病理变化。结果治疗14 d后A组与D组角膜炎症及溃疡评分差异均无统计学意义(P>0.05);B、C组角膜炎症及溃疡评分较A、D组显著降低(P<0.01),且C组角膜炎症及溃疡评分较B组显著降低(P<0.01)。病理所见给药后14 d A组和D组角膜处于炎症和溃疡期,B组角膜处于修复中期,C组角膜处于修复后期。结论温度敏感型富血小板血浆凝胶对兔角膜碱烧伤组织的修复效果良好,是一种有效的眼表修复制剂。
