To comprehensively explore the physio-biochemical and molecular changes of paclobutrazol(PBZ) at the ideal dose under water deficit stress(WDS) conditions, we investigated the effects of 100 mg/kg PBZ applied via dren...To comprehensively explore the physio-biochemical and molecular changes of paclobutrazol(PBZ) at the ideal dose under water deficit stress(WDS) conditions, we investigated the effects of 100 mg/kg PBZ applied via drenching on various physio-biochemical and molecular parameters in three rice varieties(N22, IR64, and IR64 DTY1.1) under both mild [75%-80% relative water content(RWC)] and severe(60%-65% RWC) WDS conditions. The results showed that PBZ treatment positively influenced the physio-biochemical parameters, significantly increasing dry matter(16.27%-61.91%), RWC(6.48%-16.34%), membrane stability index(4.37%-10.35%), and total chlorophyll content(8.97%-29.09%) in the rice varieties under both mild and severe WDS. Moreover, PBZ treatment reduced drought susceptibility(0.83-0.95) and enhanced drought tolerance efficiency(60.92%-86.78%), indicating its potential as a stress-mitigating agent. Global methylation analysis revealed changes in DNA methylation patterns, indicating the regulatory influence of PBZ on gene expression. The expression analysis of genes involved in the diversification of geranylgeranyl pyrophosphate towards the biosynthesis of abscisic acid, gibberellin acid, and chlorophyll showed alterations in their expression levels, suggesting the involvement of PBZ in the isoprenoid pathway. Overall, this study provides valuable insights into the potential mechanisms by which PBZ modulates physiological and molecular responses in rice plants under WDS. The findings highlight the importance of PBZ as a promising agent for enhancing drought tolerance in rice and offer valuable information for future research in crop stress management.展开更多
Paclobutrazol is a plant growth regulator and inhibitor of endogenous gibberellin synthesis.It is a powerful inhibitor of vegetative growth by changing the photosynthetic rate and plant hormone levels,thereby affectin...Paclobutrazol is a plant growth regulator and inhibitor of endogenous gibberellin synthesis.It is a powerful inhibitor of vegetative growth by changing the photosynthetic rate and plant hormone levels,thereby affecting plant growth and development.In this study,the effects of paclobutrazol on the model diatom Phaeodactylum tricornutum were investigated.Results show that 2.5-mg/L and 10-mg/L paclobutrazol significantly inhibited the algal growth by inhibiting chlorophyll synthesis,which affects photosynthesis.The antioxidant system,including catalase(CAT)and glutathione peroxidase(GPx)was severely damaged.Chrysolaminarin content was significantly elevated and doubled up to 127 mg/g dry cell weight(DCW)by 10-mg/L paclobutrazol treatment.In combination with transcriptomic analysis,paclobutrazol was demonstrated to play a regulatory role in the accumulation of chrysolaminarin and neutral lipids.展开更多
The key to high-yielding peanut cultivation is the optimization of agricultural production practices.Regulating single-seed precise sowing(SSPS)density and paclobutrazol(Pbz)application concentration are effective pra...The key to high-yielding peanut cultivation is the optimization of agricultural production practices.Regulating single-seed precise sowing(SSPS)density and paclobutrazol(Pbz)application concentration are effective practices that increase peanut yield by improving plant architecture,lodging resistance,and photosynthetic characteristics.Therefore,we conducted a two-factor field optimization experiment for the sowing density(D1:1.95×10^(5)plants ha^(-1),D52:2.40×10plants ha^(-1),D3:2.85×10^(5)plants ha^(-1),and D4:3.30×10^(5)plants ha^(-1))and Pbzapplication concentration(P0:0 mg L^(-1)and P1:100 mg L^(-1)).The objective was to optimize agricultural production practices and provide a theoretical basis for highyielding peanut cultivation by evaluating the effects of sowing density and Pbzapplication on plant architecture,lodging resistance,photosynthetic characteristics,and yield.The results showed that at the same Pbzapplication concentration,increasing sowing density increased lodging percentage and reduced leaf photosynthetic capacity.At the same sowing density,Pbzapplication reduced lodging percentage by decreasing plant height(PH),improving lignin biosynthesis-related enzyme activities,and enhancing stem puncture strength(SPS)and breaking strength(SBS).The paclobutrazol-induced alterations in plant architecture and lodging resistance improved light transmission at the middle and bottom leaf strata,resulting in the increase in relative chlorophyll content and net photosynthetic rate(Pn)of leaves.Furthermore,D3P1treatment had the highest peanut yield among all treatments.In summary,the production strategy combining the sowing density of 2.85×10^(5)plants ha^(-1)with the application of100 mg L^(-1)Pbzwas found to be the optimal agricultural production practice for giving full play to production potential and achieving higher peanut yield.展开更多
[Objectives]The paper was to explore the effects of different concentrations of paclobutrazol on seedling quality of small seedless watermelon in autumn.[Methods]The leaves of watermelon seedlings were sprayed with 30...[Objectives]The paper was to explore the effects of different concentrations of paclobutrazol on seedling quality of small seedless watermelon in autumn.[Methods]The leaves of watermelon seedlings were sprayed with 30,50,80 and 120 mg/L paclobutrazol at cotyledon expansion stage,and those sprayed with tap water were used as control.[Results]All the treatments of paclobutrazol effectively inhibited the excessive growth of watermelon at seedling stage,significantly enhanced the leaf quality of watermelon seedlings and promoted root growth.The seedlings treated by 50 mg/L paclobutrazol had the best performance compared with the control:a decrease of hypocotyl by 37.9%,an increase of plant dry weight by 46.55%,significantly increased seedling strength index,deep green leaf color,thick leaves,an increase of specific leaf weight by 28.13%,and significantly heavier root weight and longer length.[Conclusions]The results will provide a reference for strong seedling cultivation of small seedless watermelon.展开更多
[Objective] This study aimed to investigate the effect of spraying the mixture of paclobutrazol and ethephon on the endogenous hormones and carbon and nitrogen nutrients in litchi variety 'Feizixiao'.[Method] [Resul...[Objective] This study aimed to investigate the effect of spraying the mixture of paclobutrazol and ethephon on the endogenous hormones and carbon and nitrogen nutrients in litchi variety 'Feizixiao'.[Method] [Result] The results showed that foliar spraying of the mixture of paclobutrazol and ethephon could effectively inhibit litchi variety 'Feizixiao' to produce winter shoots,promote the flower bud differentiation and improve flower formation rate; meanwhile,this treatment could also increase the contents of ABA and ZR,and the ratios of ABA/IAA,ABA/GA3,ZR/IAA,ZR/GA3,and decrease the contents of IAA and GA3; additionally,it could increase the contents of soluble sugar,starch and total nitrogen,and raise C/N ratio,thus improving the flower formation rate.[Conclusion] Foliar spraying of the mixture of paclobutrazol and ethephon is an effective pathway for solving warmth damage to litchi.展开更多
Paclobutrazol was sprayed at 0, 150, and 300 mg L-1 during the closed canopy stage and the early bud stage with two high-yielding cultivars of rapeseed, Yangguang 2009 and Fengyou 520. The impact of paclobutrazol on t...Paclobutrazol was sprayed at 0, 150, and 300 mg L-1 during the closed canopy stage and the early bud stage with two high-yielding cultivars of rapeseed, Yangguang 2009 and Fengyou 520. The impact of paclobutrazol on the accumulation and distribution of biomass and its relationship with yield, resistance to lodging and pod shattering were determined. All the treatments increased the resistance as well as yield. The maximum yield was obtained when paclobutrazol was applied during the closed canopy stage at 150 mg L-1. The plant's resistance to both lodging and pod shattering was the maximum when paclobutrazol was applied during the early bud stage at 300 mg L-1. Paclobutrazol also delayed senescence, with the higher concentration or later spraying leading to more obvious effects; improved the net assimilation rate before the early bud stage; and promoted the relative growth rate of the main growth organ at each stage of growth and maximized the rate and quantities of biomass accumulation. However, at the higher concentration and later spraying, the increments were smaller. The spraying also increased the rates of biomass allocation to roots, leaves, and pods, but the rate of allocation to stems decreased as the plants grew shorter. The higher allocation to roots and the lower allocation to stems favoured resistance to both lodging and pod shattering whereas higher allocation to leaves and pods favoured yield. The higher concentration or late spraying led to excessive biomass being allocated to roots, which decreased leaf biomass during the bud stage, leading to greater resistance but lower yields.展开更多
Embryo rescue technology plays an important role in seedless grape breeding.However,the efficiency of embryo rescue,including the embryo formation,germination,and seedling rates,is closely related to the parental geno...Embryo rescue technology plays an important role in seedless grape breeding.However,the efficiency of embryo rescue,including the embryo formation,germination,and seedling rates,is closely related to the parental genotypes,degree of abortion,growth medium,and plant growth regulators.In this study,we investigated the effects of different concentrations of paclobutrazol(PAC),a plant growth regulator,and embryo collection times on the embryo formation,germination,and seedling rates for different hybrid combinations of grape breeding varieties used for their aroma and cold-resistance traits.The results showed that the different PAC concentrations had varying impacts on the development of ovules and embryos from the different grape varieties.The embryo formation rates of the‘Sultanina Rose’בBeibinghong’and‘Kunxiang Seedless’בTaishan-2’crosses were the highest under the 5.1μmol L^(-1)PAC treatment.The 1.0μmol L^(-1)PAC treatment was optimal for the germination and seedling development of the‘Sultanina Rose’בBeibinghong’embryos,whereas the 0.2μmol L^(-1)PAC treatment induced the highest germination rate for the‘Sultanina Rose’בKunxiang Seedless’cross.The optimal sampling times for each cross varied as 39 d after pollination(DAP)for the‘Flame Seedless’בMuscat Hamburg’cross,46 DAP for the‘Kunxiang Seedless’בBeibinghong’cross,and 41 DAP for the‘Ruby Seedless’בBeibinghong’and‘Fantasy Seedless’בShuangyou’crosses.Moreover,the medium modified with 0.5 g L^(-1)of indole-3-butyric acid allowed the malformed seedlings to develop into plantlets and achieve larger progenies.This study provides a useful basis for further studies into grape embryo rescue and could improve breeding efforts for new seedless grape varieties.展开更多
[Objectives] This study was conducted to investigate the effect of paclobutrazol (PBZ) on heat tolerance of dahlia.[Methods] A dahlia variety Danbanhuang was selected as the experimental material in this study. Afte...[Objectives] This study was conducted to investigate the effect of paclobutrazol (PBZ) on heat tolerance of dahlia.[Methods] A dahlia variety Danbanhuang was selected as the experimental material in this study. After 100, 200, 300 or 400 mg/L paclobutrazol was sprayed evenly to the leaves of dahlia seedlings, they were cultured in an incubator at high temperature (35 ℃/30 ℃, day/night) for two days, and then transferred to an incubator at normal temperature (25 ℃/20 ℃, day/night). The physiological and biochemical parameters of the dahlia seedlings were measured before exposure to high temperature (D0), after exposure to high temperature for two days (D2) and exposure to normal temperature for one day (R1).[Results] Compared to the negative control, foliar application of paclobutrazol decreased the content of MDA, increased the contents of chlorophyll, proline, soluble protein, and the activity of SOD, POD and CAT in dahlia plants under heat stress. The contents of MDA, soluble protein and proline in PBZ treated dahlia plants increased when they were subject to high temperature stress (35 ℃/30 ℃, day/night), and then decreased when the temperature returned to normal (25 ℃/20 ℃, day/night), and CAT activity decreased at high temperature, and then increased at normal temperature, while the activity of SOD and POD kept rising during the entire experimental period.[Conclusions] Foliar application of paclobutrazol can alleviate the adverse effect caused by high temperature to dahlia plants, and the experimental data provide a theoretical basis for the application of paclobutrazol in dahlia cultivation in South China.展开更多
In order to study the effects of seed soaking with Paclobutrazol( PP333) on the tillering and physiological characteristics of sugarcane seedlings,sugar cane variety ROC22 was used as experimental material. The effect...In order to study the effects of seed soaking with Paclobutrazol( PP333) on the tillering and physiological characteristics of sugarcane seedlings,sugar cane variety ROC22 was used as experimental material. The effects of seed soaking with different concentrations of PP333 were studied. The tiller number and growth of sugarcane were surveyed at the 6-leaf stage,the physiological indicators such as chlorophyll content,soluble protein content,proline content and peroxidase activity were measured at the 2-leaf,4-leaf,and 6-leaf stages. The results showed that the seed soaking with PP333 can effectively improve the tillering of sugarcane seedlings. The suitable concentration for the tillering of sugarcane and the growth and development of sugarcane was of 50 mg/L; the seed soaking with PP333 significantly increased the chlorophyll,soluble protein,proline,and peroxidase activity of leaves of sugarcane seedlings; the content of chlorophyll and soluble protein in leaves of sugarcane seedlings treated with 90 mg/L PP333 was the highest,and the proline content and peroxidase activity of sugarcane treated with 50 mg/L PP333 were the highest.展开更多
[Objectives] The research aimed to study the effects of applying biofertilizer instead of paclobutrazol on the content of flavonoids in Ophiopogon japonicas and determine its quality characteristics,in order to provid...[Objectives] The research aimed to study the effects of applying biofertilizer instead of paclobutrazol on the content of flavonoids in Ophiopogon japonicas and determine its quality characteristics,in order to provide the scientific basis for the quality control of GAP and the comprehensive utilization of resources. [Methods] O. japonicas collected in Lingxing Town and Huayuan Town of Sichuan Province was divided into three groups: blank control group,paclobutrazol group and biofertilizer group. Root tuber of O. japonicas was obtained,and the enzyme was killed by the microwave,and then it was dried in 60℃. The content of flavonoids in root tuber of O. japonicus was determined by ultraviolet spectrophotometry. Then,SPSS19. 0 was used to analyze the results. [Results]The average contents of flavonoids in different treatments were as follows: 0. 184%-0. 198% in control group; 0. 283%-0. 330% in the paclobutrazol group; 0. 404%-0. 428% in the biofertilizer group. Biological bacterial fertilizer had a significant role in promoting the synthetic accumulation of homoisoflavonoids in O. japonicus( P <0. 001**). [Conclusions]The application of biological fertilizer can not only significantly improve the content of homoisoflavonoids in root ruber of O. japonicus,but also significantly optimize the micro ecological environment of soil. Therefore,biofertilizer should be widely used to gradually replace paclobutrazol in the main production areas of O. japonicas.展开更多
In angiosperms, low red (R)/far-red (FR) ratio light increases gibberellin (GA) levels. GA signaling in conifer seedlings requires FR to promote shoot elongation and reduce the inhibition of shoot elongation induced b...In angiosperms, low red (R)/far-red (FR) ratio light increases gibberellin (GA) levels. GA signaling in conifer seedlings requires FR to promote shoot elongation and reduce the inhibition of shoot elongation induced by paclobutrazol (PAC), yet the effects of far-red light in inducing shoot elongation in the presence or absence of PAC is poorly understood. In this study, transcriptomic and proteomic analyses was used to examine the molecular mechanism of FR regulation of shoot elongation in Chinese pine (Pinus tabuliformis Carr.) seedlings in the presence of PAC. Four treatments were compared: white light + water (WW), FR + water (RW), white light + PAC (WP), and FR + PAC (RP), and 1436 differentially expressed genes (DEGs) and 450 differentially expressed proteins (DEPs) were identified in RW_WW (RW contrast WW), and 1862 DEGs and 481 DEPs in RP_WP (RP contrast WP). Metabolic and signal transduction pathway analyses of DEGs and DEPs in RW_WW and RP_WP, indicated that the former required more energy than the latter. Moreover, gibberellic acid, auxin, and brassinolide are equally important in RW_WW and RP_WP for shoot elongation, except for the ethylene pathway. Amino acid metabolism and cell wall organization were significantly enriched in RW_WW and RP_WP, respectively. In summary, RW_WW and RP_WP had different effects in secondary metabolism, energy metabolism, amino acid metabolism, cell wall organization, and hormone response. These results provide an important theoretical and reference basis for studying the regulatory effect of low R/FR and PAC in conifer shoot elongation.展开更多
[ Objective] The paper was to explore the effect of seed soaking with paclobutrazol ( PP333 ) on early tillering and endogenous hormone content of sugarcane seedlings. [ Method ] With the sugarcane variety ROC22 as ...[ Objective] The paper was to explore the effect of seed soaking with paclobutrazol ( PP333 ) on early tillering and endogenous hormone content of sugarcane seedlings. [ Method ] With the sugarcane variety ROC22 as the experimental material, seeds were soaked in different concentrations of PP333- The number and growth of tiller were investigated, and the contents of endogenous hormones IAA, CTK, ABA and GA3 at two-leaf, four-loaf and six-leaf stages of sugarcane seedlings were measured, to study the effects of seed soaking with PP333 on early filleting and endogenous hormone contents of sugarcane seedlings. [Result] Seed soaking with PP333 could effectively improve and advance filleting occurrence of sugarcane seedlings. The suitable PP333 concentration for sugarcane fiUeting occurrence, growth and development was 50 mg/L. Seed soaking with PP333 significantly increased the CTK and ABA contents in leaves of sugarcane seedlings, and decreased IAA and GA3 contents. [ Conclusion]The paper will provide a theoretical basis for application of PP333 in sugarcane production.展开更多
Three japonica rice varieties were soaked in 110 mg/L paclobutrazol solution for 48 h and then directly seeded, and seeds soaked with clean water were used as a control group. The analysis of dry weights of various or...Three japonica rice varieties were soaked in 110 mg/L paclobutrazol solution for 48 h and then directly seeded, and seeds soaked with clean water were used as a control group. The analysis of dry weights of various organs and agronomic traits showed that soaking of japonica seeds with paclobutrazol had little impact on the correlation between dry weights of various organs, but there were still significant differences between cultivars. The correlation coefficients between lengths of the first and second nodes with filled grain weight changed from 0.024 and 0.056 in the CK to -0.116 and -0.042 correspondingly after presoaking with paclobutrazol. Therefore, paclobutrezol reduces the effect of stem-sheath weight on filled grain weight, and as a result, photosynthetic product could not be effectively transported in maturation stage of rice.展开更多
Area of cultivating garlic in Indonesia experienced a sharp decline. In a period of 20 years (1991-2011), the decline of garlic planting area reaches 91%. The fact is causing erosion of genetic resources of several ...Area of cultivating garlic in Indonesia experienced a sharp decline. In a period of 20 years (1991-2011), the decline of garlic planting area reaches 91%. The fact is causing erosion of genetic resources of several varieties of garlic. Propagated vegetatively garlic through in vitro culture aims to obtain quality bulblet. This study aims to get the combination treatment between paclobutrazol, indole acetic acid (IAA), indole butyric acid (IBA) and naphthalene acetic acid (NAA) in some varieties of garlic in Indonesia. This study used a completely randomized design (CRD) with two treatment factors, i.e., on varieties (Bali, Gunung Kidul, Slawi, Tawangmangu and Mataram) and 1.5 ppm paclobutrazol combination with a wide auxin (IAA 0.5 ppm, 0.5 ppm IBA, NAA 0.5 ppm). Variable observations include the current appear shoots and roots, number of shoots and roots, shoots and root length. Observations showed that the combination of paclobutrazol + NAA is able to produce plantlets that have the best vigor than paclohutrazol + IAA and paclobutrazol + IBA combination treatment.展开更多
Besides being employed as an efficient plant growth retardant for field, garden and potted plants, paclobutrazol (PBZ) is also used in laboratory experiments, mainly in aseptic cultures, both in autoclaved and non-aut...Besides being employed as an efficient plant growth retardant for field, garden and potted plants, paclobutrazol (PBZ) is also used in laboratory experiments, mainly in aseptic cultures, both in autoclaved and non-autoclaved form. Therefore it is not known if autoclaving can partial or completely inactivates the product, thus decreasing its efficacy. Thus a simple experiment was carried out to assess to what extent the autoclaving process can affect some growth components and dry mass accumulation and partition in sunflower seedlings, by employing the techniques of plant Growth Analysis. Autoclaving did not modify either qualitative or quantitatively any of the plant responses to PBZ as well their reversions by gibberellic acid.展开更多
文摘To comprehensively explore the physio-biochemical and molecular changes of paclobutrazol(PBZ) at the ideal dose under water deficit stress(WDS) conditions, we investigated the effects of 100 mg/kg PBZ applied via drenching on various physio-biochemical and molecular parameters in three rice varieties(N22, IR64, and IR64 DTY1.1) under both mild [75%-80% relative water content(RWC)] and severe(60%-65% RWC) WDS conditions. The results showed that PBZ treatment positively influenced the physio-biochemical parameters, significantly increasing dry matter(16.27%-61.91%), RWC(6.48%-16.34%), membrane stability index(4.37%-10.35%), and total chlorophyll content(8.97%-29.09%) in the rice varieties under both mild and severe WDS. Moreover, PBZ treatment reduced drought susceptibility(0.83-0.95) and enhanced drought tolerance efficiency(60.92%-86.78%), indicating its potential as a stress-mitigating agent. Global methylation analysis revealed changes in DNA methylation patterns, indicating the regulatory influence of PBZ on gene expression. The expression analysis of genes involved in the diversification of geranylgeranyl pyrophosphate towards the biosynthesis of abscisic acid, gibberellin acid, and chlorophyll showed alterations in their expression levels, suggesting the involvement of PBZ in the isoprenoid pathway. Overall, this study provides valuable insights into the potential mechanisms by which PBZ modulates physiological and molecular responses in rice plants under WDS. The findings highlight the importance of PBZ as a promising agent for enhancing drought tolerance in rice and offer valuable information for future research in crop stress management.
基金Supported by the National Natural Science Foundation of China (Nos.31870027,42006125)the Guangdong Natural Science Foundation (No.2019B1515120062)。
文摘Paclobutrazol is a plant growth regulator and inhibitor of endogenous gibberellin synthesis.It is a powerful inhibitor of vegetative growth by changing the photosynthetic rate and plant hormone levels,thereby affecting plant growth and development.In this study,the effects of paclobutrazol on the model diatom Phaeodactylum tricornutum were investigated.Results show that 2.5-mg/L and 10-mg/L paclobutrazol significantly inhibited the algal growth by inhibiting chlorophyll synthesis,which affects photosynthesis.The antioxidant system,including catalase(CAT)and glutathione peroxidase(GPx)was severely damaged.Chrysolaminarin content was significantly elevated and doubled up to 127 mg/g dry cell weight(DCW)by 10-mg/L paclobutrazol treatment.In combination with transcriptomic analysis,paclobutrazol was demonstrated to play a regulatory role in the accumulation of chrysolaminarin and neutral lipids.
基金supported by the National Key Research and Development Program of China(2020YFD1000902)the Shandong Key Research and Development Program(2018YFJH0601-3)+1 种基金the Major Agricultural Applied Technological Innovation Projects in Shandong Province(SD2019ZZ11)the Shandong Modern Agricultural Technology and Industry System(SDAIT-04-01)。
文摘The key to high-yielding peanut cultivation is the optimization of agricultural production practices.Regulating single-seed precise sowing(SSPS)density and paclobutrazol(Pbz)application concentration are effective practices that increase peanut yield by improving plant architecture,lodging resistance,and photosynthetic characteristics.Therefore,we conducted a two-factor field optimization experiment for the sowing density(D1:1.95×10^(5)plants ha^(-1),D52:2.40×10plants ha^(-1),D3:2.85×10^(5)plants ha^(-1),and D4:3.30×10^(5)plants ha^(-1))and Pbzapplication concentration(P0:0 mg L^(-1)and P1:100 mg L^(-1)).The objective was to optimize agricultural production practices and provide a theoretical basis for highyielding peanut cultivation by evaluating the effects of sowing density and Pbzapplication on plant architecture,lodging resistance,photosynthetic characteristics,and yield.The results showed that at the same Pbzapplication concentration,increasing sowing density increased lodging percentage and reduced leaf photosynthetic capacity.At the same sowing density,Pbzapplication reduced lodging percentage by decreasing plant height(PH),improving lignin biosynthesis-related enzyme activities,and enhancing stem puncture strength(SPS)and breaking strength(SBS).The paclobutrazol-induced alterations in plant architecture and lodging resistance improved light transmission at the middle and bottom leaf strata,resulting in the increase in relative chlorophyll content and net photosynthetic rate(Pn)of leaves.Furthermore,D3P1treatment had the highest peanut yield among all treatments.In summary,the production strategy combining the sowing density of 2.85×10^(5)plants ha^(-1)with the application of100 mg L^(-1)Pbzwas found to be the optimal agricultural production practice for giving full play to production potential and achieving higher peanut yield.
基金Beijing Innovation Consortium of Special Crops Research System(BAIC04-2022).
文摘[Objectives]The paper was to explore the effects of different concentrations of paclobutrazol on seedling quality of small seedless watermelon in autumn.[Methods]The leaves of watermelon seedlings were sprayed with 30,50,80 and 120 mg/L paclobutrazol at cotyledon expansion stage,and those sprayed with tap water were used as control.[Results]All the treatments of paclobutrazol effectively inhibited the excessive growth of watermelon at seedling stage,significantly enhanced the leaf quality of watermelon seedlings and promoted root growth.The seedlings treated by 50 mg/L paclobutrazol had the best performance compared with the control:a decrease of hypocotyl by 37.9%,an increase of plant dry weight by 46.55%,significantly increased seedling strength index,deep green leaf color,thick leaves,an increase of specific leaf weight by 28.13%,and significantly heavier root weight and longer length.[Conclusions]The results will provide a reference for strong seedling cultivation of small seedless watermelon.
文摘[Objective] This study aimed to investigate the effect of spraying the mixture of paclobutrazol and ethephon on the endogenous hormones and carbon and nitrogen nutrients in litchi variety 'Feizixiao'.[Method] [Result] The results showed that foliar spraying of the mixture of paclobutrazol and ethephon could effectively inhibit litchi variety 'Feizixiao' to produce winter shoots,promote the flower bud differentiation and improve flower formation rate; meanwhile,this treatment could also increase the contents of ABA and ZR,and the ratios of ABA/IAA,ABA/GA3,ZR/IAA,ZR/GA3,and decrease the contents of IAA and GA3; additionally,it could increase the contents of soluble sugar,starch and total nitrogen,and raise C/N ratio,thus improving the flower formation rate.[Conclusion] Foliar spraying of the mixture of paclobutrazol and ethephon is an effective pathway for solving warmth damage to litchi.
基金funded by the National Key Technology Research and Development Program of China(2014BAD11B03)the earmarked fund for the China Agricultural Research System(CARS-13)the Fundamental Research Funds for the Central Universities of China(2013PY001)
文摘Paclobutrazol was sprayed at 0, 150, and 300 mg L-1 during the closed canopy stage and the early bud stage with two high-yielding cultivars of rapeseed, Yangguang 2009 and Fengyou 520. The impact of paclobutrazol on the accumulation and distribution of biomass and its relationship with yield, resistance to lodging and pod shattering were determined. All the treatments increased the resistance as well as yield. The maximum yield was obtained when paclobutrazol was applied during the closed canopy stage at 150 mg L-1. The plant's resistance to both lodging and pod shattering was the maximum when paclobutrazol was applied during the early bud stage at 300 mg L-1. Paclobutrazol also delayed senescence, with the higher concentration or later spraying leading to more obvious effects; improved the net assimilation rate before the early bud stage; and promoted the relative growth rate of the main growth organ at each stage of growth and maximized the rate and quantities of biomass accumulation. However, at the higher concentration and later spraying, the increments were smaller. The spraying also increased the rates of biomass allocation to roots, leaves, and pods, but the rate of allocation to stems decreased as the plants grew shorter. The higher allocation to roots and the lower allocation to stems favoured resistance to both lodging and pod shattering whereas higher allocation to leaves and pods favoured yield. The higher concentration or late spraying led to excessive biomass being allocated to roots, which decreased leaf biomass during the bud stage, leading to greater resistance but lower yields.
基金supported by the Key Industry Chain of Innovation Project in Shaanxi Province, China (2021ZDLNY04–08)the Fundamental Research Funds for the Central Universities, China (2452019016)+1 种基金the China Agriculture Research System of MOF and MARA (CARS–30-yz-7)the Ningxia Hui Autonomous Region Flexible Introduction of Scientific and Technological Innovation Team, China (2020RXTDLX08)
文摘Embryo rescue technology plays an important role in seedless grape breeding.However,the efficiency of embryo rescue,including the embryo formation,germination,and seedling rates,is closely related to the parental genotypes,degree of abortion,growth medium,and plant growth regulators.In this study,we investigated the effects of different concentrations of paclobutrazol(PAC),a plant growth regulator,and embryo collection times on the embryo formation,germination,and seedling rates for different hybrid combinations of grape breeding varieties used for their aroma and cold-resistance traits.The results showed that the different PAC concentrations had varying impacts on the development of ovules and embryos from the different grape varieties.The embryo formation rates of the‘Sultanina Rose’בBeibinghong’and‘Kunxiang Seedless’בTaishan-2’crosses were the highest under the 5.1μmol L^(-1)PAC treatment.The 1.0μmol L^(-1)PAC treatment was optimal for the germination and seedling development of the‘Sultanina Rose’בBeibinghong’embryos,whereas the 0.2μmol L^(-1)PAC treatment induced the highest germination rate for the‘Sultanina Rose’בKunxiang Seedless’cross.The optimal sampling times for each cross varied as 39 d after pollination(DAP)for the‘Flame Seedless’בMuscat Hamburg’cross,46 DAP for the‘Kunxiang Seedless’בBeibinghong’cross,and 41 DAP for the‘Ruby Seedless’בBeibinghong’and‘Fantasy Seedless’בShuangyou’crosses.Moreover,the medium modified with 0.5 g L^(-1)of indole-3-butyric acid allowed the malformed seedlings to develop into plantlets and achieve larger progenies.This study provides a useful basis for further studies into grape embryo rescue and could improve breeding efforts for new seedless grape varieties.
基金Supported by Applied Basic Research Project of Suzhou City(SYN201405)
文摘[Objectives] This study was conducted to investigate the effect of paclobutrazol (PBZ) on heat tolerance of dahlia.[Methods] A dahlia variety Danbanhuang was selected as the experimental material in this study. After 100, 200, 300 or 400 mg/L paclobutrazol was sprayed evenly to the leaves of dahlia seedlings, they were cultured in an incubator at high temperature (35 ℃/30 ℃, day/night) for two days, and then transferred to an incubator at normal temperature (25 ℃/20 ℃, day/night). The physiological and biochemical parameters of the dahlia seedlings were measured before exposure to high temperature (D0), after exposure to high temperature for two days (D2) and exposure to normal temperature for one day (R1).[Results] Compared to the negative control, foliar application of paclobutrazol decreased the content of MDA, increased the contents of chlorophyll, proline, soluble protein, and the activity of SOD, POD and CAT in dahlia plants under heat stress. The contents of MDA, soluble protein and proline in PBZ treated dahlia plants increased when they were subject to high temperature stress (35 ℃/30 ℃, day/night), and then decreased when the temperature returned to normal (25 ℃/20 ℃, day/night), and CAT activity decreased at high temperature, and then increased at normal temperature, while the activity of SOD and POD kept rising during the entire experimental period.[Conclusions] Foliar application of paclobutrazol can alleviate the adverse effect caused by high temperature to dahlia plants, and the experimental data provide a theoretical basis for the application of paclobutrazol in dahlia cultivation in South China.
基金Supported by Project of National Natural Science Foundation(31501362)Natural Science Foundation Project of Guangxi(2014GXNSFBA118289,2014GXNSFAA118090)
文摘In order to study the effects of seed soaking with Paclobutrazol( PP333) on the tillering and physiological characteristics of sugarcane seedlings,sugar cane variety ROC22 was used as experimental material. The effects of seed soaking with different concentrations of PP333 were studied. The tiller number and growth of sugarcane were surveyed at the 6-leaf stage,the physiological indicators such as chlorophyll content,soluble protein content,proline content and peroxidase activity were measured at the 2-leaf,4-leaf,and 6-leaf stages. The results showed that the seed soaking with PP333 can effectively improve the tillering of sugarcane seedlings. The suitable concentration for the tillering of sugarcane and the growth and development of sugarcane was of 50 mg/L; the seed soaking with PP333 significantly increased the chlorophyll,soluble protein,proline,and peroxidase activity of leaves of sugarcane seedlings; the content of chlorophyll and soluble protein in leaves of sugarcane seedlings treated with 90 mg/L PP333 was the highest,and the proline content and peroxidase activity of sugarcane treated with 50 mg/L PP333 were the highest.
基金Supported by Science and Technology Support Plan Project of Sichuan Science and Technology Department(2015SZ0035,2015SZ0033)
文摘[Objectives] The research aimed to study the effects of applying biofertilizer instead of paclobutrazol on the content of flavonoids in Ophiopogon japonicas and determine its quality characteristics,in order to provide the scientific basis for the quality control of GAP and the comprehensive utilization of resources. [Methods] O. japonicas collected in Lingxing Town and Huayuan Town of Sichuan Province was divided into three groups: blank control group,paclobutrazol group and biofertilizer group. Root tuber of O. japonicas was obtained,and the enzyme was killed by the microwave,and then it was dried in 60℃. The content of flavonoids in root tuber of O. japonicus was determined by ultraviolet spectrophotometry. Then,SPSS19. 0 was used to analyze the results. [Results]The average contents of flavonoids in different treatments were as follows: 0. 184%-0. 198% in control group; 0. 283%-0. 330% in the paclobutrazol group; 0. 404%-0. 428% in the biofertilizer group. Biological bacterial fertilizer had a significant role in promoting the synthetic accumulation of homoisoflavonoids in O. japonicus( P <0. 001**). [Conclusions]The application of biological fertilizer can not only significantly improve the content of homoisoflavonoids in root ruber of O. japonicus,but also significantly optimize the micro ecological environment of soil. Therefore,biofertilizer should be widely used to gradually replace paclobutrazol in the main production areas of O. japonicas.
基金This work was supported by the National Natural Science Foundation of China(31770713)Biosafety and Genetic Resources Management Project of State Forestry and Grassland Administration(KJZXSA202030).
文摘In angiosperms, low red (R)/far-red (FR) ratio light increases gibberellin (GA) levels. GA signaling in conifer seedlings requires FR to promote shoot elongation and reduce the inhibition of shoot elongation induced by paclobutrazol (PAC), yet the effects of far-red light in inducing shoot elongation in the presence or absence of PAC is poorly understood. In this study, transcriptomic and proteomic analyses was used to examine the molecular mechanism of FR regulation of shoot elongation in Chinese pine (Pinus tabuliformis Carr.) seedlings in the presence of PAC. Four treatments were compared: white light + water (WW), FR + water (RW), white light + PAC (WP), and FR + PAC (RP), and 1436 differentially expressed genes (DEGs) and 450 differentially expressed proteins (DEPs) were identified in RW_WW (RW contrast WW), and 1862 DEGs and 481 DEPs in RP_WP (RP contrast WP). Metabolic and signal transduction pathway analyses of DEGs and DEPs in RW_WW and RP_WP, indicated that the former required more energy than the latter. Moreover, gibberellic acid, auxin, and brassinolide are equally important in RW_WW and RP_WP for shoot elongation, except for the ethylene pathway. Amino acid metabolism and cell wall organization were significantly enriched in RW_WW and RP_WP, respectively. In summary, RW_WW and RP_WP had different effects in secondary metabolism, energy metabolism, amino acid metabolism, cell wall organization, and hormone response. These results provide an important theoretical and reference basis for studying the regulatory effect of low R/FR and PAC in conifer shoot elongation.
基金Supported by National Natural Science Foundation of China(31501362)Natural Science Foundation of Guangxi Province(2014GXNSFBA118289,2014GXNSFAA118090,2014GXNSFBA118068,2015GXNSFAA139063)Development Fund of Guangxi Academy of Agricultural Science(2015JZ13,2015JZ98,2014YQ01)
文摘[ Objective] The paper was to explore the effect of seed soaking with paclobutrazol ( PP333 ) on early tillering and endogenous hormone content of sugarcane seedlings. [ Method ] With the sugarcane variety ROC22 as the experimental material, seeds were soaked in different concentrations of PP333- The number and growth of tiller were investigated, and the contents of endogenous hormones IAA, CTK, ABA and GA3 at two-leaf, four-loaf and six-leaf stages of sugarcane seedlings were measured, to study the effects of seed soaking with PP333 on early filleting and endogenous hormone contents of sugarcane seedlings. [Result] Seed soaking with PP333 could effectively improve and advance filleting occurrence of sugarcane seedlings. The suitable PP333 concentration for sugarcane fiUeting occurrence, growth and development was 50 mg/L. Seed soaking with PP333 significantly increased the CTK and ABA contents in leaves of sugarcane seedlings, and decreased IAA and GA3 contents. [ Conclusion]The paper will provide a theoretical basis for application of PP333 in sugarcane production.
文摘Three japonica rice varieties were soaked in 110 mg/L paclobutrazol solution for 48 h and then directly seeded, and seeds soaked with clean water were used as a control group. The analysis of dry weights of various organs and agronomic traits showed that soaking of japonica seeds with paclobutrazol had little impact on the correlation between dry weights of various organs, but there were still significant differences between cultivars. The correlation coefficients between lengths of the first and second nodes with filled grain weight changed from 0.024 and 0.056 in the CK to -0.116 and -0.042 correspondingly after presoaking with paclobutrazol. Therefore, paclobutrezol reduces the effect of stem-sheath weight on filled grain weight, and as a result, photosynthetic product could not be effectively transported in maturation stage of rice.
文摘Area of cultivating garlic in Indonesia experienced a sharp decline. In a period of 20 years (1991-2011), the decline of garlic planting area reaches 91%. The fact is causing erosion of genetic resources of several varieties of garlic. Propagated vegetatively garlic through in vitro culture aims to obtain quality bulblet. This study aims to get the combination treatment between paclobutrazol, indole acetic acid (IAA), indole butyric acid (IBA) and naphthalene acetic acid (NAA) in some varieties of garlic in Indonesia. This study used a completely randomized design (CRD) with two treatment factors, i.e., on varieties (Bali, Gunung Kidul, Slawi, Tawangmangu and Mataram) and 1.5 ppm paclobutrazol combination with a wide auxin (IAA 0.5 ppm, 0.5 ppm IBA, NAA 0.5 ppm). Variable observations include the current appear shoots and roots, number of shoots and roots, shoots and root length. Observations showed that the combination of paclobutrazol + NAA is able to produce plantlets that have the best vigor than paclohutrazol + IAA and paclobutrazol + IBA combination treatment.
基金Thanks are due to FAPEMIG(Foundation for Research Sponsoring of Minas Gerais State)for the scholarships granted to DMR(Post-Doctoral training),JB and GBR(Scientific Initiation training)and for the financial support in several occasions.
文摘Besides being employed as an efficient plant growth retardant for field, garden and potted plants, paclobutrazol (PBZ) is also used in laboratory experiments, mainly in aseptic cultures, both in autoclaved and non-autoclaved form. Therefore it is not known if autoclaving can partial or completely inactivates the product, thus decreasing its efficacy. Thus a simple experiment was carried out to assess to what extent the autoclaving process can affect some growth components and dry mass accumulation and partition in sunflower seedlings, by employing the techniques of plant Growth Analysis. Autoclaving did not modify either qualitative or quantitatively any of the plant responses to PBZ as well their reversions by gibberellic acid.