An adjustable mixer for surface acoustic wave( SAW)-less radio frequency( RF) front-end is presented in this paper. Through changing the bias voltage,the presented mixer with reconfigurable voltage conversion gain( VC...An adjustable mixer for surface acoustic wave( SAW)-less radio frequency( RF) front-end is presented in this paper. Through changing the bias voltage,the presented mixer with reconfigurable voltage conversion gain( VCG) is suitable for multi-mode multi-standard( MMMS) applications. An equivalent local oscillator( LO) frequency-tunable high-Q band-pass filter( BPF) at low noise amplifier( LNA) output is used to reject the out-of-band interference signals. Base-band( BB) capacitor of the mixer is variable to obtain 15 kinds of intermediate frequency( IF) bandwidth( BW). The proposed passive mixer with LNA is implemented in TSMC 0. 18μm RF CMOS process and operates from 0. 5 to 2. 5 GHz with measured maximum out-of-band rejection larger than 40 d B. The measured VCG of the front-end can be changed from 5 to 17 d B; the maximum input intercept point( IIP3) is0 d Bm and the minimum noise figure( NF) is 3. 7 d B. The chip occupies an area of 0. 44 mm^2 including pads.展开更多
单对线以太网是近年来新兴的以太网技术,随着汽车自动驾驶和工业物联网的高速发展,凭借上层应用扩展和底层布线上的绝对优势,正在大规模应用。单对线以太网物理层模拟前端技术是实现单对线以太网通信的关键基础技术。本文讲述了现有单...单对线以太网是近年来新兴的以太网技术,随着汽车自动驾驶和工业物联网的高速发展,凭借上层应用扩展和底层布线上的绝对优势,正在大规模应用。单对线以太网物理层模拟前端技术是实现单对线以太网通信的关键基础技术。本文讲述了现有单对线以太网物理层模拟前端相关的标准,架构及相关模块设计技术,重点对发射器TX和接收器RX关键模块的现有实现技术及其优缺点进行了列举分析。发射器TX电流模结构易于实现高精度但功耗效率低,电压模结构精度略低但功耗效率更高;接收器RX的设计围绕模拟数字转换器(Analog-to-Digital Converter,ADC)展开,ADC决定着整个RX的性能、功耗、面积和复杂度,分段和重新装配(Segmentation And Reassembly,SAR)ADC是首选结构,应用上限不断提高。由此进一步明确了在高性能、低功耗、小面积的单对线以太网物理层模拟前端设计中的挑战。展开更多
This paper presents a low power tunable active inductor and RF band pass filter suitable for multiband RF front end circuits. The active inductor circuit uses the PMOS cascode structure as the negative transconductor ...This paper presents a low power tunable active inductor and RF band pass filter suitable for multiband RF front end circuits. The active inductor circuit uses the PMOS cascode structure as the negative transconductor of a gyrator to reduce the noise voltage. Also, this structure provides possible negative resistance to reduce the inductor loss with wide inductive bandwidth and high resonance frequency. The RF band pass filter is realized using the proposed active inductor with suitable input and output buffer stages. The tuning of the center frequency for multiband operation is achieved through the controllable current source. The designed active inductor and RF band pass filter are simulated in 180 nm and 45 nm CMOS process using the Synopsys HSPICE simulation tool and their performances are compared. The parameters, such as resonance frequency, tuning capability, noise and power dissipation, are analyzed for these CMOS technologies and discussed. The design of a third order band pass filter using an active inductor is also presented.展开更多
Panax ginseng C. A. Meyer is an important traditional herb in eastern Asia. It contains ginsenosides, which are primary bioactive compounds with medicinal properties. Although ginseng has been cultivated since at leas...Panax ginseng C. A. Meyer is an important traditional herb in eastern Asia. It contains ginsenosides, which are primary bioactive compounds with medicinal properties. Although ginseng has been cultivated since at least the Ming dynasty to increase production, cultivated ginseng has lower quantities of ginsenosides and lower disease resistance than ginseng grown under natural conditions. We extracted root RNA from six varieties of fifth-year P. ginseng cultivars representing four different growth conditions, and performed Illumina paired-end sequencing. In total, 163,165,706 raw reads were obtained and used to generate a de novo transcriptome that consisted of 151,763 contigs(76,336 unigenes), of which 100,648 contigs(66.3%) were successfully annotated. Differential expression analysis revealed that most differentially expressed genes(DEGs) were upregulated(246 out of 258, 95.3%) in ginseng grown under natural conditions compared with that grown under artificial conditions. These DEGs were enriched in gene ontology(GO) terms including response to stimuli and localization. In particular, some key ginsenoside biosynthesis-related genes, including HMG-Co A synthase(HMGS), mevalonate kinase(MVK), and squalene epoxidase(SE), were upregulated in wild-grown ginseng. Moreover, a high proportion of disease resistance-related genes were upregulated in wild-grown ginseng. This study is the first transcriptome analysis to compare wild-grown and cultivated ginseng, and identifies genes that may produce higher ginsenoside content and better disease resistance in the wild; these genes may have the potential to improve cultivated ginseng grown in artificial environments.展开更多
The latent expression pattern of Epstein-Barr Virus （EBV）igenes in nasopharyngeal carcinoma （NPC） has been extensively investigated, and the expression of several lytic genes in NPC has been reported. However, com...The latent expression pattern of Epstein-Barr Virus （EBV）igenes in nasopharyngeal carcinoma （NPC） has been extensively investigated, and the expression of several lytic genes in NPC has been reported. However, comprehensive information through EBV transcriptome analysis in NPC is limited. We performed paired-end RNA-seq to systematically and comprehensively characterize the expression of EBV genes in NPC tissue and C666-1 NPC cell line, which consistently carries EBV. In addition to the transcripts restricted to type II latency infection, the type Ⅲ latency EBNA3s genes and a substantial number of lytic genes, such as BZLF1, BRLF1, and BMRF1, were detected through RNA-seq and were further verified in C666-1 cells and NPC tissue through real- time PCR. We also performed clustering analysis to classify NPC patient groups in terms of EBV gene expression, which presented two subtypes of NPC samples. Results revealed interesting patterns of EBV gene expression in NPC patients. This clustering was correlated with many signaling pathways, such as those related to heterotrimeric G-protein signaling, inflammation mediated by chemokine and cytokine signaling, ribosomes, protein metabolism, influenza infection, and ECM-receptor interaction. Our combined findings suggested that the expression of EBV genes in NPC is restricted not only to type II latency genes but also to type Ⅲ latency and lyric genes. This study provided further insights into the potential role of EBV in the development of NPC.展开更多
基金Supported by the National Basic Research Program of China(No.2010CB327404)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘An adjustable mixer for surface acoustic wave( SAW)-less radio frequency( RF) front-end is presented in this paper. Through changing the bias voltage,the presented mixer with reconfigurable voltage conversion gain( VCG) is suitable for multi-mode multi-standard( MMMS) applications. An equivalent local oscillator( LO) frequency-tunable high-Q band-pass filter( BPF) at low noise amplifier( LNA) output is used to reject the out-of-band interference signals. Base-band( BB) capacitor of the mixer is variable to obtain 15 kinds of intermediate frequency( IF) bandwidth( BW). The proposed passive mixer with LNA is implemented in TSMC 0. 18μm RF CMOS process and operates from 0. 5 to 2. 5 GHz with measured maximum out-of-band rejection larger than 40 d B. The measured VCG of the front-end can be changed from 5 to 17 d B; the maximum input intercept point( IIP3) is0 d Bm and the minimum noise figure( NF) is 3. 7 d B. The chip occupies an area of 0. 44 mm^2 including pads.
文摘单对线以太网是近年来新兴的以太网技术,随着汽车自动驾驶和工业物联网的高速发展,凭借上层应用扩展和底层布线上的绝对优势,正在大规模应用。单对线以太网物理层模拟前端技术是实现单对线以太网通信的关键基础技术。本文讲述了现有单对线以太网物理层模拟前端相关的标准,架构及相关模块设计技术,重点对发射器TX和接收器RX关键模块的现有实现技术及其优缺点进行了列举分析。发射器TX电流模结构易于实现高精度但功耗效率低,电压模结构精度略低但功耗效率更高;接收器RX的设计围绕模拟数字转换器(Analog-to-Digital Converter,ADC)展开,ADC决定着整个RX的性能、功耗、面积和复杂度,分段和重新装配(Segmentation And Reassembly,SAR)ADC是首选结构,应用上限不断提高。由此进一步明确了在高性能、低功耗、小面积的单对线以太网物理层模拟前端设计中的挑战。
文摘This paper presents a low power tunable active inductor and RF band pass filter suitable for multiband RF front end circuits. The active inductor circuit uses the PMOS cascode structure as the negative transconductor of a gyrator to reduce the noise voltage. Also, this structure provides possible negative resistance to reduce the inductor loss with wide inductive bandwidth and high resonance frequency. The RF band pass filter is realized using the proposed active inductor with suitable input and output buffer stages. The tuning of the center frequency for multiband operation is achieved through the controllable current source. The designed active inductor and RF band pass filter are simulated in 180 nm and 45 nm CMOS process using the Synopsys HSPICE simulation tool and their performances are compared. The parameters, such as resonance frequency, tuning capability, noise and power dissipation, are analyzed for these CMOS technologies and discussed. The design of a third order band pass filter using an active inductor is also presented.
基金supported by the International Science and Technology Cooperation of China(2011DFA32730)
文摘Panax ginseng C. A. Meyer is an important traditional herb in eastern Asia. It contains ginsenosides, which are primary bioactive compounds with medicinal properties. Although ginseng has been cultivated since at least the Ming dynasty to increase production, cultivated ginseng has lower quantities of ginsenosides and lower disease resistance than ginseng grown under natural conditions. We extracted root RNA from six varieties of fifth-year P. ginseng cultivars representing four different growth conditions, and performed Illumina paired-end sequencing. In total, 163,165,706 raw reads were obtained and used to generate a de novo transcriptome that consisted of 151,763 contigs(76,336 unigenes), of which 100,648 contigs(66.3%) were successfully annotated. Differential expression analysis revealed that most differentially expressed genes(DEGs) were upregulated(246 out of 258, 95.3%) in ginseng grown under natural conditions compared with that grown under artificial conditions. These DEGs were enriched in gene ontology(GO) terms including response to stimuli and localization. In particular, some key ginsenoside biosynthesis-related genes, including HMG-Co A synthase(HMGS), mevalonate kinase(MVK), and squalene epoxidase(SE), were upregulated in wild-grown ginseng. Moreover, a high proportion of disease resistance-related genes were upregulated in wild-grown ginseng. This study is the first transcriptome analysis to compare wild-grown and cultivated ginseng, and identifies genes that may produce higher ginsenoside content and better disease resistance in the wild; these genes may have the potential to improve cultivated ginseng grown in artificial environments.
基金This study was supported by 973 Program and 863 Program from the Ministry of Science and Technology of China （Nos. 2011CB504300 and 2015AA020931）, National Natural Science Foundation of China （Nos. 91440106, 91019015, and 81302037）, Ph.D. Startup of Guangzhou Medical University （No. 2012C65）, Guangzhou Science and Technology Planing Project （No. 2014J4100181）, and Young Teacher Training Program ofStm Yat-sen University （No. 13ykpy50）.
文摘The latent expression pattern of Epstein-Barr Virus （EBV）igenes in nasopharyngeal carcinoma （NPC） has been extensively investigated, and the expression of several lytic genes in NPC has been reported. However, comprehensive information through EBV transcriptome analysis in NPC is limited. We performed paired-end RNA-seq to systematically and comprehensively characterize the expression of EBV genes in NPC tissue and C666-1 NPC cell line, which consistently carries EBV. In addition to the transcripts restricted to type II latency infection, the type Ⅲ latency EBNA3s genes and a substantial number of lytic genes, such as BZLF1, BRLF1, and BMRF1, were detected through RNA-seq and were further verified in C666-1 cells and NPC tissue through real- time PCR. We also performed clustering analysis to classify NPC patient groups in terms of EBV gene expression, which presented two subtypes of NPC samples. Results revealed interesting patterns of EBV gene expression in NPC patients. This clustering was correlated with many signaling pathways, such as those related to heterotrimeric G-protein signaling, inflammation mediated by chemokine and cytokine signaling, ribosomes, protein metabolism, influenza infection, and ECM-receptor interaction. Our combined findings suggested that the expression of EBV genes in NPC is restricted not only to type II latency genes but also to type Ⅲ latency and lyric genes. This study provided further insights into the potential role of EBV in the development of NPC.
