Permselectivity of 4－Aminophenol，Paracetanol and Phenacetin on the Self－Assembled n－Alkanethiol Monolayer Modified Gold Electrode

The gold electrodes coated by n-alkanethiol with various chain lengths were used to study the permeability of uric acid,ascorbic acid,4-aminophenol,paracetanol and phenacetin by means of linear sweep voltammetry.The results show that the optimum chain length is n=10.The improvements in the selectivity and the stability of the amperometric detection of these compounds in a flow stream were obtained by n-alkanethiol self assembled monolayers modified electrodes based on their differences in the hydrophobicity and the permeability.

荧光分光光度法研究对乙酰氨基酚与牛血清白蛋白之间的相互作用

在模拟人体生理环境下,用荧光分光光度法及紫外-可见分光光度法研究了解热镇痛药对乙酰氨基酚(简写作PTL)与牛血清蛋白(BSA)之间的相互反应。试验结果发现:由于非辐射能量转移引起的荧光猝灭,BSA的荧光强度因与PTL相互之间的结合反应而明显降低。按Stern-Volmer方程和Lineweaver-Burk方程对所得实际数据进行了处理,求得此结合反应的静态平衡常数(KLB)为2.592×103mol.L-1(297 K),其结合位置距212位色氨酸1.94 nm。根据结合反应的热力学参数,推断PTL与BSA之间的结合力为疏水作用力。

高效液相色谱法测定小儿氨酚黄那敏颗粒中对乙酰氨基酚的含量

目的建立小儿氨酚黄那敏颗粒中对乙酰氨基酚的测定方法。方法采用Eclipse XDB-C18(4.6 mm×150 mm,5μm)色谱柱,以甲醇-水(40∶60)为流动相,流速1.0 ml.min-1,检测波长275 nm,进样量为20μl。结果对乙酰氨基酚在5.84～58.4 mg.L-1(r=0.999 8)内线性良好,平均回收率为116.6%,RSD=1.46%。结论该法简便快速、重复性好,可用于该制剂的质量控制。