Development and parentage analysis of SNP markers for Chestnut-vented Nuthatch(Sitta nagaensis)based on ddRAD-seq data

Extra-pair paternity(EPP)is commonly found in socially monogamous birds,especially in small passerine birds,and there are interspecific and intraspecific variations in the extent of EPP.The Chestnut-vented Nuthatch(Sitta nagaensis)is a socially monogamous passerine bird,and verifying whether this species has EPP relies on parentage testing-S.nagaensis is not known to have EPP.In this study,we developed SNP markers of this species that are informative for parentage analysis from double digest restriction site-associated DNA sequencing(ddRAD-seq)data.A panel consisting of 50 SNP markers,with a mean heterozygosity of 0.343,was used to resolve 95% of nestlings to fathers.The combined exclusion probabilities for the first parent and second parent were 0.991 and 0.9999,respectively.This panel of SNP markers is a powerful tool for parentage assignments in S.nagaensis.In addition,we found that three offspring(7.9%)from three nests(23.1%)were the result of extra-pair fertilization out of 38 offspring in 13 nests.Our study provided information on parentage analysis that has not been reported before in S.nagaensis.It also supplemented the understudied EPP behavior of birds in Asia,contributing to a general understanding of the EPP behaviors of birds.

A high level of extra-pair paternity in the Chestnut Thrush(Turdus rubrocanus)

Extra-pair copulation(EPC)can potentially maximize individual reproductive fitness,and this process may involve sexual selection of male and female traits that reflect individual quality.Previous studies have implied that adult characteristics are associated with the probability of extra-pair paternity(EPP),but it differs between species.Moreover,there are relatively few examples of the adaptive rationale for females’engagement in EPCs based on an examination of these more traditionally recorded traits,in combination with female flight-mediated traits such as wing length.We investigated whether EPP existed in the wild Chestnut Thrush(Turdus rubrocanus)population during three breeding seasons(2019–2021),and whether paternity was related to morphological traits of males and females.Eight highly variable microsatellite loci were used to identify paternity,and generalized linear mixed models were used to analyze the relationship between paternity and morphological traits.We found that EPP existed in the Chestnut Thrush.53.3%(N=41/77)of the broods contained at least one extra-pair offspring(EPO),and 34.6%(N=72/208)of the nestlings were EPO.We also found that male wing length was negatively associated with the probability of EPP and the proportion of EPO.Female body length was positively related to the probability of EPP.Both female body condition and mass were negatively associated with the proportion of EPO.While other traits of male and female did not relate to the probability of EPP or the proportion of EPO.Extra-pair males had better body condition compared to the males they cuckolded.EPO did not differ from their half-siblings in terms of body size or body condition.The results suggest that body size and body condition were associated with EPP in the Chestnut Thrush.This study provides fundamental information for further studies on the evolution and maintenance of EPP in the Chestnut Thrush,and it is also useful for the comparison of EPP among Turdus species.

87例亲权鉴定案例分析

1987年5月至1991年3月,我室共接受亲权鉴定案87例,占我室同期总鉴定例数的44%,现将资料整理报告如下。材料与方法一、材料来源87例亲权鉴定案中,62例由湖北、湖南、河南、江西、江苏省各地、县、市及武汉地区司法机关送检,占71.3%,22例为自诉案件,占25.3%,其余3例由主管计划生育部门委托鉴定,占3.4%。

Emancipatory reflection on a nursing practice-based ethical issue about nurses' paternalistic decision-making for patients

Objective: This study aimed to demonstrate and promote the skill of critical emancipatory reflection through reflecting on a nursing practice-based ethical issue about nurses' paternalistic decision-making for patients. Meanwhile, critical awareness will be developed and the underlying issues of paternalism in nursing decision-making will be analyzed. Then, by applying the procedure, improvement in nursing decision-making practice will be expected.Methods: Taylor's model of emancipatory reflection with four steps, including construction, deconstruction, confrontation, and reconstruction, is utilized to guide the author's reflection.Results: Guided by the socialization theory, the author's personal and professional socialization is seen to be associated with the formation of the value of paternalism. The theory of reflexivity is applied to unearth the related issues, including deeper personal value,work environment, as well as historical and cultural contexts. Moreover, the power derived from policy, work relationship, and nursing administration, which could induce paternalism in the author's nursing decision-making practice, was critically debated using the hegemony theory. Finally, new insights into paternalism will be achieved, which enable change in terms of how to facilitate patients' autonomous decision-making.Conclusions: The process of refection makes it clear that respecting patients' right and performing patient-centered caring are the bases to change the paternalism existing in the nursing decision-making practice currently. The reconstruction step assists the author in terms of how to value the patients' autonomy and balance patients' safety and choice, rather than being overprotective; carry out risk assessment, and search for strong evidence to counterbalance the positive and negative aspects of risk-taking; communicate with patients appropriately in a manner that they can comprehend; spend more time to explore patients' preference and choice; make every effort to elevate the patients' decision-making capacity; implement patient-centered care and shared decision-making in nursing practice; consult with other colleagues and obtain the required support when limitations or challenges exist; try to justify and avoid hidden paternalism behind policy or guidelines; deal with the power in hand well and fairly; and also positively face the powers that constrain the author.

Is Hiding Success a Good Idea?A Systematic Review of Reasons and Costs of Hiding Success

Hiding success,which is defined as intentionally withholding positive information about oneself or one’s accomplishments,is a common phenomenon and culturally recognized.It has been found that hiding success is guided by paternalistic motives and it may have social costs.Although the consequences of hiding success within many different types of relationships are slightly different,sharing success is better than hiding success.From the point of view of explaining the reasons and costs of hiding success,a systematic review was conducted in this paper.There have been some limitations of the current literature,such as the lack of systematic theoretical explanation of the mechanism of hiding success.We recommended that future research should explore deeply from the aspects of a real-world setting,the communication medium and choice of words,the relationship between the communicator and target,culture and so on.

Paternò-Büchi反应的区域选择性

Paternò-Büchi(P-B)反应,即羰基-烯的[2+2]光环化加成反应,利用其特殊的区域及立体选择性可以合成一些结构精巧的取代氧杂环丁烷。随着P-B反应在有机合成中越来越广泛的应用,人们对P-B反应的区域选择性的研究也越来越深入。在P-B反应发现之初,人们一直用“最稳定的双自由基规则”解释其区域选择性,这一规则对有些体系却不适用,近年来有人开始运用“自旋化学”理论来解释区域选择性。本论文结合作者的研究工作,评述了近年来P-B反应的区域选择性的研究进展,重点阐述了反应温度、反应物的取代基对P-B反应区域选择性的影响及其规律。

年长男子的生育年龄对生育质量的影响

Women have been increasingly delaying the start of motherhood in recent decades. The same trend is seen also for men. The influence of maternal age on fertility, chromosomal anomalies, pregnancy complications, and impaired perinatal and post-natal outcome of offspring, has been thoroughly investigated, and these aspectsare clinically applied during fertility and pregestational counseling. Male aging and reproductive outcome has gained relatively less attention. The purpose of this review is to evaluate updated and relevant literature on the effect of paternal age on reproductive outcome.

高龄对男性生育力的影响

Modern societal pressures and expectations over the past several decades have resulted in the tendency for couples to delay conception. While women experience a notable decrease in oocyte production in their late thirties, the effect of age on spermatogenesis is less well described. While there are no known limits to the age at which men can father children, the effects of advanced paternal age are incompletely understood. This review summarizes the current state of knowledge regarding advanced paternal age and its implications on semen quality, reproductive success and offspring health. This review will serve as a guide to physicians in counseling men about the decision to delay paternity and the risks involved with conception later in life.

Growth of eight Pacific abalone families at three temperatures

Growth rates, measured as shell length and body weight daily growth, were studied in the eight families of Pacific abalone, Haliotis discus hannai Ino, reared at 12, 16 and 20 ℃for 40 d respectively. The results show that J1Rh family grew the best at 12 ℃, with growth rates of (32.88±4.66) μm/d and (5.24±1.84) mg/d. C1Jm family had the highest growth rates of (58.00±2.00) μm/d and (9.71±1.21) mg/d at 16 ℃. J1Jm family ranked the first at 20 ℃, with growth rates of (66.00±1.76) μm/d and (10.99±0.34) mg/d. RjRh family had the slowest growth rates at all three temperatures. Shell length growth rates were 18.25, 33.00 and 43.13 μm/d respec- tively, while body weight growth rates were 2.47, 2.56 and 4.75 mg/d respectively. Both temperature and family had significant effect on growth rates (P< 0.05). At 16 and 20 ℃, maternal effects on growth rates were not significant (P> 0.05), but paternal effects on growth rates were significant (P< 0.05). Results of this study indicate genetic difference among the families and importance of select- ing male breeders in the commercial hatchery.

Establishment of paternity testing system using microsatellite markers in Chinese Holstein

To estimate the efficiency of microsatellite markers in paternity testing among Chinese Holstein, 30 microsatellite loci were used to differentiate 330 Chinese Holstein genotypes, according to the calculation of the allele frequency, number of alleles, effective number of alleles, genetic heterozygosity, polymorphic information content （PIC）, and the exclusion probability in this cattle population. The results demonstrated that the exclusion probability ranged from 0.620 in locus BM1818 to 0.265 in locus INRA005 with the average of 0.472 and 11 microsatellite markers exceeding 0.5. The combined exclusion probability of nine microsatellite markers was over 0.99. The result showed that paternity testing of Chinese Holstein was basically resolved using the nine microsatellite markers selected.

Developmental Toxicity in Mice Following Paternal Exposure to Di-N-Butyl-Phthalate (DBP)

Objective The aim of the present study was to investigate the effects of paternal Di‐N‐butyl‐phthalate （DBP） exposure pre‐ and postnatally on F1 generation offspring,and prenatally on F2 generation offspring.Methods Male mice were exposed to either 500 mg/kg or 2 000 mg/kg of DBP for 8 weeks,and mated with non‐exposed females.Three‐quarters of the females were sacrificed a day prior to parturition,and examined for the number of living and dead implantations,and incidence of gross malformations.Pups from the remaining females were assessed for developmental markers,growth parameters,as well as sperm quantity and quality.Results There were no changes in the fertility of parents and in intrauterine development of the offspring.Pups of DBP‐exposed males demonstrated growth‐retardation.Following paternal exposure to 500 mg/kg bw of DBP,there were almost twice the number of males than females born in the F1 generation.F1 generation females had a 2.5‐day delay in vaginal opening.Paternal exposure to 2 000 mg/kg bw of DBP increased the incidence of sperm head malformations in F1 generation males;however,there were no changes in the fertility and viability of foetuses in the F2 generation.Conclusion Paternal DBP exposure may disturb the sex ratio of the offspring,delay female sexual maturation,and deteriorate the sperm quality of F1 generation males.

中国人群尿道下裂发病相关危险因素的研究

This case-controlled study was designed to evaluate the association between various baseline parental factors and the risk of hypospadias in China. Patients were selected from tertiary referral hospitals in Anhui, a province in mid-eastern China. A questionnaire was given to the parents of each patient. The final database included 193 cases and 835 controls. The incidence of additional coexistent anomalies was 13.0%, primarily cryptorchidism （9.8%）. Ten patients （5.1%） were from families with genital anomaly, including five families （2.6%） with hypospadias. The risks of hypospadias was higher for children of mothers 〉 35 （odds ratio [OR] =1.47） and 〈 18 （OR = 2.95） years of age, and in mothers who had consumed alcohol （OR = 2.67）, used drugs （OR = 1.53） and had an infection （OR = 1.87） during pregnancy. The risk of hypospadias was also higher when mothers （OR = 1.68） and fathers （OR = 1.74） were engaged in agriculture. Other factors assessed were not associated with the risk of hypospadias.

High level of extra-pair paternity in the socially monogamous Marsh Tits(Poecile palustris)

Background:Extra-pair copulation behavior has been widely studied among socially monogamous birds.Many species revealed high rates of extra-pair paternity.But few of the studies have been carried out in the Asian population.Methods:From 2012 to 2019,we explored the extra-pair paternity of Marsh Tits(Poecile palustris)in Xianrendong National Nature Reserve,Liaoning Province,China.During the study,adult Marsh Tits were captured with mist nets and parental birds,with nest-box traps.Blood samples were taken from the brachial vein.Parentage analyses were carried out using nine highly variable microsatellite loci through Cervus 3.0 software and maximum likelihood approach.Results:Forty-nine offspring(15.08%)from 20 nests(45.45%)were the results of extra-pair fertilization out of a total of 325 offspring in 44 nests.The average extra-pair offspring ratio was 33.54%,with a set varying from 11.11 to 71.43%.Nine extra-pair fathers had been successfully identified,four of whom were the close neighbors of the focus nest while the nests of the remaining five were relatively far.No significant difference was found in the genetic similarity between the social and extra-pair mates of the female,nor in the heterozygosis among the maternal half-siblings.Conclusions:In general,our study proved that the extra-pair paternity in Marsh Tits and its extra-pair mating is independent of the genetic compatibility hypothesis.This complements the understudied bird's extra-pair paternity in Asian area and contributes to the comprehensive insight of birds'extra-pair paternity behaviors.

Genetic Analysis of Multiple Paternity in an Endangered Ovoviviparous Lizard Shinisaurus crocodilurus

The crocodile lizard （Shinisaurus croeodilurus） is an ovoviviparoug lizard belonging to a monotypic family that originated during the end of the quaternary ice age. A rare species in the wild, the crocodile lizard was listed in CITES Appendix II. Knowledge of the reproductive biology and mating system of this species is important for designing conservation strategies and improving genetic variation. To investigate the paternity of the crocodile lizards and to interpret their reproductive behaviour, we collected saliva from females, potential fathers and offspring in a semi- natural enclosure experiment and analyzed the paternity of the crocodile lizard using 12 microsatellite genetic loci. The overall observed incidence of multiple paternity was 42.9% （6 of 14 clutches） and Fis was 0.089 ± 0.056. These results indicate that the primary mating mode of the crocodile lizard is that males are polygynous while with females are polyandrous, and there is multiple paternity among offspring of the same mother.

Mode of pollen spread in clonal seed orchard of Pinus koraiensis

The patterns of effective pollen dispersal and the relationships between pollen dispersal and genetic composition in Pinus koraiensis are still unclear. Hence, we investigated the mode of pollen dispersal of P. koraiensis in a clonal seed orchard in Lushuihe Forestry Bureau, Jilin Province, using SSR molecular markers tech-nique and the method of maximum likelihood. A total of 13 pairs of nuclear microsatellites po-lymorphic primers were used in the paternity analysis. We analyzed 100 progenies and 150 paternities. A total of 56 alleles were detected in 13 loci with 3-6 alleles (4.3 alleles in average) in a single locus. The primers of SsrPt_ctg7170 and SsrPt_ctg5333 had the maximum (6) and the minimum (3) alleles, respectively. The averaged values of observed heterozygosity, expected heterozygosity and polymorphism information content of experimental groups were 0.648, 0.601 and 0.533, respectively. The mating dis-tance of P. koraiensis followed an approximately normal distribution. The most effective pollen for the female parent came from male parent trees 15-45 m away with an average mating dis-tance of 32.60 m and the longest of 67.88 m. The pollen source of offspring was not random but greatly affected by the wind direction during the pollination season of P. koraiensis. These re-sults have important implications for seed or-chard design to improve the genetic quality of seeds and seed production.

QTL and genetic analysis controlling fiber quality traits using paternal backcross population in upland cotton

Background:Genetic improvement in fiber quality is one of the main challenges for cotton breeders.Quantitative trait loci(QTL)mapping provides a powerful approach to dissect the molecular mechanism in fiber quality traits.In present study,F14 recombinant inbred line(RIL)population was backcrossed to paternal parent for a paternal backcross(BC/P)population,deriving from one upland cotton hybrid.Three repetitive BC/P field trials and one maternal backcross(BC/M)field trial were performed including both two BC populations and the original RIL population.Results:In total,24 novel QTLs are detected for fiber quality traits and among which 13 QTLs validated previous results.Thirty-five QTLs in BC/P populations explain 5.01%–22.09%of phenotype variation(PV).Among the 35 QTLs,23 QTLs are detected in BC/P population alone.Present study provides novel alleles of male parent for fiber quality traits with positive genetic effects.Particularly,qFS-Chr3–1 explains 22.09%of PV in BC/P population,which increaseds 0.48 cN·tex−1 for fiber strength.A total of 7,2,8,2 and 6 QTLs explain over 10.00%of PV for fiber length,fiber uniformity,fiber strength,fiber elongation and fiber micronaire,respectively.In RIL population,six common QTLs are detected in more than one environment:qFL-Chr1–2,qFS-Chr5–1,qFS-Chr9–1,qFS-Chr21–1,qFM-Chr9–1 and qFM-Chr9–2.Two common QTLs of qFE-Chr2–2(TMB2386-SWU12343)and qFM-Chr9–1(NAU2873-CGR6771)explain 22.42%and 21.91%of PV.The region between NAU4034 and TMB1296 harbor 30 genes(379 kb)in A05 and 42 genes(49 kb)in D05 for fiber length along the QTL qFL-Chr5–1 in BC/P population,respectively.In addition,a total of 142 and 46 epistatic QTLs and QTL×environments(E-QTLs and QQEs)are identified in recombinant inbred lines in paternal backcross(RIL-P)and paternal backcross(BC/P)populations,respectively.Conclusions:The present studies provide informative basis for improving cotton fiber quality in different populations.

Mutation Analysis of STR Locus on 23 Autosomes in Hainan Population

[Objective] To analyze the mutation signature and regularity of STR locus on 23 autosomes in paternity testing cases in Hainan. [Methods] A total of 2715 paternity testing cases accepted by the Forensic Medical Identification Centre of our hospital from 2017 to 2020 derived from counties and cities in Hainan Province were collected, the cases containing gene mutations were selected, the mutation rate and details of each locus were counted, and the mutation regu-larity of 23 STR loci was analyzed. [Results] Of the 2715 cases identified as “support”, 1487 were triplet cases and 1640 were dyad cases, totaling 4614 meioses;There were 50 gene mutation events (including 17 triplet mutations and 33 dyad mutations), with an average mutation rate of 0.0047% and a cumulative mutation rate of 1.0837%. A total of 19 of the 23 STR loci were mutated, with a mutation rate of 0.1301% at the D12S391 locus and 0.0217% at five loci, TPOX, D1S1656, D2S441, D22S1045, and PentaD, while no muta-tion events were found at four loci, D19S433, TH01, D13S317, and D7S820. Of the 50 mutation events, 47 were one-step mutations, 1 was two-step, and 2 were three-step. There were 35 paternal mutations (13 triplets and 22 dyads), 6 maternal mutations (4 triplets and 2 dyads), and 9 indeterminate pater-nal/maternal mutations, with a paternal to maternal mutation ratio of 5.83:1. [Conclusion] The mutation rate of D12S391 locus is the highest, and the muta-tion rate of TPOX, D1S1656, D2S441, D22S1045 and PentaD loci is the lowest in Hainan population, and paternal mutations are more than maternal muta-tions. In the paternity test, if 1 - 3 STR loci do not conform to the genetic law, especially when the mutant locus is homozygous or the next of kin is identi-fied, it is necessary to use other kits to review and increase the number of loci or use the second-generation sequencing technology to confirm, carefully de-termine the mutation and ensure the accuracy of the identification conclusion.

Behavioral problems and related factors in children of different aged patients with schizophrenia: A cross-sectional study

BACKGROUND： With the age growing, changes of behavioral problems in children whose parents had schizophrenia, differences of related factors and ways for particular intervention should be further studied. OBJECTIVE： To survey the behavioral problems in children of different aged patients with chizophrenia and investigate the correlation between behavioral problems and related factors in different aged groups. DESIGN： Cross-sectional study. SETTING： Shandong Mental Health Center. PARTICIPANTS： A total of 364 patients with schizophrenia were selected from eleven psychiatric hospitals from June 1999 to June 2000. There were 179 males and 185 females, and their ages ranged from 28 to 45 years. All patients met modified diagnostic criteria of schizophrenia （the second edition）, China Classification of Mental Diseases and Diagnostic Criteria. Meanwhile, children of the 364 patients were grouped based on their ages, including 6 - 1 l years old group [n -217; 114 males and 103 females; mean age of （9±2） years] and 12 - 16 years old group [n = 147; 99 males and 48 females; mean age of （14±1） years]. The Chinese norms of Achenbach＇s Child Behavior Check List were regarded as the normal control group. METHODS： Children who received self-made mental health related factors inventory and Achenbach＇s Child Behavior Check List were involved in this study. All children and their parents provided the confirmed consent. Achenbach＇s Child Behavior Check List was used for parents to mainly evaluate children, and the results manifested various behavioral problems based on different sexes and different ages. Self-made mental health related factors inventory contained questionnaires for parents and children, respectively. In this study, home situation of parents as well as personality characteristics and educational styles of children were mainly evaluated. MAIN OUTCOME MEASURES： ① Scores of Achenbach＇s Child Behavior Check List in different aged children; ② Scores of self-made mental health related factors inventory based on different home situations and educational styles. RESULTS： Children of 364 patients were involved in the final analysis. ① There were no significant differences in total score of Achenbach＇s Child Behavior Check List and sex in the two groups （P 〉 0.05）. There were significant differences in schizoid, body recount, aggression, depression, hyperactivity, violating discipline, compulsion and total abnormal rate of boys between the 6 - 11 years old group and the normal control group （P 〈 0.05 - 0.01）. In addition, there were also significant differences in poor communication, depression, hyperactivity, violating discipline, compulsion and total abnormal rate of girls between the 6 - 11 years old group and the normal control group （P 〈 0.05 - 0.01）. ② As compared with normal control group,scores of home environment in the two aged groups demonstrated that educational level was low; interpersonal relationship was poor; relation to children was not closed; relationship between husband and wife was not harmonious; positive rate of family history was high; economic status was poor; family atmosphere was poor. Therefore, there was significant difference （P 〈 0.01）. Educational level in parental generation and single parent family in the 6 - 11 years old group were higher than that in the 12 - 16 years old group, with significant difference （P 〈 0.05）. As compared with the normal control group, results of education demonstrated that children in the two aged groups had poor communication with other children; they had poor behaviors in schools; most of them were boarded and had poor relationship with their parents; teaching styles for children were different. CONCLUSION： All children of different aged patients with schizophrenia had poor home environment and education; and they were often affected by other factors. Behavioral problems were changed with the age growing; in addition, these changes were related to home environment, education and society. Therefore, comprehensive intervention may be an ideal method.

Cannabis and male reproduction:Impact on offspring via sperm epigenome

Cannabis is the most widely used drug in theWestern societies particularly among adolescent and young adults.Epidemiological studies demonstrate that men use cannabis more frequently than women with higher risk in developing cannabis-related disorders.Although the direct adverse effects of cannabis on male reproductive functions have been studied both in humans and animal models,the possible long-term risks for the health of the users and their offspring are still an area of active research.

Mate choice for major histocompatibility complex(MHC)complementarity in the Yellow-rumped Flycatcher(Ficedula zanthopygia)

Background:Genes of the major histocompatibility complex(MHC)are an important component of the vertebrate immune system and play a significant role in mate choice in animal populations.However,the MHC genetic targets of female mate choice have not been clearly identified,and whether female mate choice is based on neutral genetic characteristics remains an open question.Here,we focus on the effects of morphological traits and genetic similarity among individuals in MHC class IIB(MHC IIB)exon 2 on mating in a sexually dimorphic songbird that exhibits social monogamy with extra-pair paternity(EPP).Methods:We sequenced 64 parent-offspring triads sampled over a 3-year period using two MHC class II loci to detect disassortative mating in the Yellow-rumped Flycatcher(Ficedula zanthopygia).Results:We found that MHC similarity in social pairs was lower than that in random pairs.Extra-pair mate choice according to MHC IIB was observed,in which females’extra-pair mates had fewer MHC alleles than their within-pair mates,but there was no significant band-sharing between extra-pair sires and potential extra-pair mates.However,the interaction between the MHC diversity of females and that of the social males affected the occurrence of EPP.Conclusions:Our results support the“optimality hypothesis”of MHC-based social and extra-pair choice.Female choice probably maintains a certain level of MHC diversity in offspring in the Yellow-rumped Flycatcher.