Investigating the Genetic Bases of Growth Regulation by E2F3 in Dwarf Surf Clams Mulinia lateralis

Bivalve aquaculture plays a crucial role in the aquaculture industry due to the economic value of many bivalve species.Understanding the underlying genetic basis of bivalve growth regulation is essential for enhancing germplasm innovation and ensuring sustainable development of the industry.Though numerous candidate genes have been identified,their functional validation remains challenging.Fortunately,the dwarf surf clam(Mulinia lateralis)serves as a promising model organism for investigating genetic mechanisms underlying growth regulation in bivalves.The GWAS study in the Yesso scallop(Patinopecten yessoensis)has pinpointed the E2F3 gene as a key regulator of growth-related traits.However,the specific role of E2F3 in bivalve growth remains unclear.This study aimed to further confirm the regulatory function of the E2F3 gene in the dwarf surf clam through RNA interference experiments.Our results revealed several genes are associated with individual growth and development,including CTS7,HSP70B2,and PGLYRP3,as well as genes involved in lipid metabolism such as FABP2 and FASN.Functional enrichment analysis indicated that E2F3 primarily modulates critical processes like amino acid and lipid metabolism.These findings suggest that E2F3 likely regulates growth in the dwarf surf clam by influencing amino acid and lipid metabolism.Overall,this study advances our understanding on the function of E2F3 gene in growth regulation in bivalves,providing valuable insights for future research in this field.

Impact of an official accountability audit on industrial structure adjustment:a case study of environmental regulation in China

As a unique environmental regulation in China,the official accountability audit was piloted in 2014.With a focus on prioritizing the ecological environment,officials in pilot districts have implemented economic construction,adjusted industrial structures,and promoted coordinated development between the economy and environment.The effects of implementation have garnered widespread attention from society.However,there is limited research on the impact of an accountability audit on industrial structure adjustments.Using the“Accountability Audit of Officials for Natural Resource Assets(Trial)”released in 2015 as a quasi-natural experiment,this study collected panel data from 279 cities between 2013 and 2017.It then empirically analyzed the impact mechanism and effects of the accountability audit on industrial structure adjustment using the Propensity Score Matching and Difference-in-Differences model.The research findings indicate that the accountability audit directly impacted industrial structure adjustment,promoting the upgrading of the primary industry to the secondary industry and restricting the development of the tertiary industry.In addition,the audit is beneficial for enterprise entry,but not conducive to technological innovation,and has no significant impact on foreign direct investment.This conclusion fills a gap in the existing research and provides valuable insights for policymakers.

Exploring the relationship between red blood cell levels and emotional regulation through the miR191-Riok3-Mxi1 pathway

Objective:To assess emotional fluctuations,physical and mental health status,and indicators closely related to red blood cells,such as RIO kinase 3(Riok3),MAX interactor 1(Mxi1),and microRNA 191(miR191),in participants with different levels of red blood cells.Methods:Participants who underwent physical examinations at Dongfang Hospital between April and October 2019 were divided into healthy,blood deficiency,and anemia groups(30 individuals in the healthy and blood deficiency group respectively,and 13 in the anemia group).The physical and mental conditions of the participants were evaluated through questionnaires,and emotional fluctuations were assessed through an emotion-inducing experiment,in which participants watched video segments designed to induce specific emotions.Relative expression levels of miR191,Riok3,and Mxi1 from venous blood samples were also determined.Results:The main psychological factors identified in the anemia and blood deficiency groups were obsessive-compulsive symptoms,depression,anxiety,and other negative emotions.Relative gene expression levels indicated that miR191 was upregulated and Riok3 and Mxi1 were downregulated in both the blood deficiency and anemia groups.Regarding the emotional score of disgust on video stage,the main effect was significant(F=335.58,P<.001),which showed that watching the three videos caused participants to have a dominant emotion,and there is a difference on group(F=5.35,P=.01),with higher disgust scores in the anemia and blood deficiency groups.The symptoms of blood deficiency and anemia,such as weakness in limbs were significantly negatively correlated with Riok3 and Mxi1 expression(r=-0.38 and-0.31 respectively),but was significantly positively correlated with miR191 expression(r=0.29).Conclusion:We determined that a close relationship exists between red blood cell levels and emotional status.Our findings suggest that individuals with anemia and blood deficiency are more likely to experience psychological problems and negative emotions,particularly disgust.We also demonstrate that emotional regulation is related to mir191-Riok3-Mxi1 pathway activity,identifying these pathway components are potential targets for genetic therapies in combination with psychological therapy.

Environmental regulation and firms'performance:a quasi-natural experiment from China

This paper investigates whether environmental regulation affects firm's performance including productivity,wage and employment using firm-level data in 1996-2007.The identification uses Two Control Zones(TCZ)policy implemented by the Chinese government in 1998,in which tougher environmental regulations were imposed in TCZ cities but not others.By difference-indifferences estimation,we find that tougher environmental regulation leads to larger increase on both total factor productivity and wage,and firms tend to employ fewer workers under pressure of environmental protection.Heterogeneous tests show that the impacts are better for nonpollution intensive firms,non-exporters and SOEs.Finally,we find that R&D input and exiting firms among industry are the driving forces for enhancing TFP and increasing wage and unemployment.

Regulation of vegetation pattern on the hydrodynamic processes of erosion on hillslope in Loess Plateau,China

As vegetation are closely related to soil erosion,hydrodynamic parameter changes under various vegetation pattern conditions can be used as an important basis for the research of the soil erosion mechanism.Through upstream water inflow experiments conducted on a loess hillslope,how the vegetation pattern influences the hydrodynamic processes of sediment transport was analyzed.The results show that the placement of a grass strip on the lower upslope can effectively reduce runoff erosion by 69%,relying on the efficiency of regulated hydrodynamic process.The effective location of grass strip for hillslope alleviating erosion is on the lower part of the upslope,mainly due to the grass strip measure used to regulate the hydrodynamic system.As a result,the underlying surface runoff resistance is increased by 5 times,runoff shear stress is decreased by more than 90%,and runoff power decreased by over 92%.The measure greatly separates the scouring energy of surface runoff that acts on the slope soil.Therefore,the use of grass strips effectively decreases the energy of runoff flowing along the slope,eliminating soil erosion to a great extent and thereby achieving a better regulation of hydrodynamic processe.

Experimental and Numerical Study on the Gas Explosion in Urban Regulator Station

Regulator station is an important part in the urban gas transmission and distribution system.Once gas explosion occurs,the real explosion process and consequences of methane gas explosion in the regulator station were not revealed systematically.In this study,a full-scale experiment was carried out to simulate the regulator station explosion process,and some numerical simulations with a commercial CFD software called FLACS were conducted to analyze the effect of ignition and vent conditions on the blast overpressure and flame propagation.The experimental results demonstrated that the peak overpressure increased as the distance from the vent increased within a certain distance.And the maximum overpressure appeared 3 m away from the door,which was about 36.6 kPa.It was found that the pressure-time rising curves obtained from the simulation are basically the same as the ones from the experiment,however,the time of reaching the peak pressure was much shorter.The numerical simulation results show that the peak overpressures show an increase trend as the ignition height decreased and the vent relief pressure increased.It indicates that the damage and peak overpressure of gas explosion could be well predicted by FLACS in different styles of regulator station.In addition,the results help us to understand the internal mechanism and development process of gas explosion better.It also offers technical support for the safety protection of the urban regulator station.

Application of"balancing digestive functions by regulating pivot"in chronic atrophic gastritis and professor Wei Wei's clinical experience

Chronic atrophic gastritis(CAG)has attracted wide attention in clinic due to its high incidence rate and high canceration rate.At present,modern medicine lacks an effective treatment scheme for CAG.Traditional Chinese medicine can improve clinical symptoms,laboratory indexes,pathological examination results and patients'quality of life for patients of CAG.Professor Wei used the method of"Balancing Digestive Functions by Regulating Pivot"to treat CAG in clinical practice.With flexible addition and subtraction on the basic prescription Banxia Xiexin decoction and coordination with various external treatment methods and psychological construction,professor Wei has achieved satisfactory clinical effects with the pungent-opening and bitter-descending method and dispersing liver and promoting blood circulation method.

Affect regulation in psychoanalytic treatments of patients with a borderline personality disorder–psychoanalysis and psychodynamic psychotherapy–a comparison

BACKGROUND A recent meta-analysis has confirmed that the effects of psychotherapy on patients with borderline personality disorders(BPD)are still insufficiently understood.Evidence of differences between different types of therapies has been questioned.AIM To study repetitive interaction patterns in patients with BPD undergoing either psychoanalysis or psychodynamic therapy.METHODS Psychoanalysis(PSA)or psychodynamic psychotherapy(PDT)was administered to 10 patients each,the two groups were matched.Therapy regimens were applied according to care as usual/manualized including quality control and supervision as usual.Randomization to one of the groups was done after baseline assessment.During classical PSA(n=10)and PDT(n=10),semiannually,recordings(audio or video)of five consecutive therapy sessions were taken over three years for an ex-post analysis.The patients'characteristics,such as affect parameters[Affect regulation and experience Q-sort(AREQ)],quality of object relations(quality of object relations scale)and personality traits[Shedler-Westen Assessment Procedure(SWAP-200)]were analyzed retrospectively by independent raters.Therapeutic action(psychotherapy process Q-sort)and affective(re)actions of the patients(AREQ)were then analyzed in relation to changes found in the patients'characteristics.RESULTS During the first year of therapy(PSA:n=10;PDT:n=9),the therapeutic method PSA was associated with significant improvements in the variable"SWAP Borderline",while in PDT change was not significantly different to baseline(PSA:P=0.04;PDT:P=0.33).Long-term results and follow up was available for seven participants in PSA and for five in PDT after three years;change in SWAP borderline for the whole sample was not significant at this time point when confronting to baseline(P=0.545).However,differences between PSA and PDT were significant when analyzing the“mean change”in the SWAP Borderline variable after one year of therapy(P=0.024):PSA led to slightly increased BPD symptoms,while PDT to a decrease;for the long run,variance of observed change was higher in PSA than in PDT(SDPSA±9.29 vs SDPDT±7.94).Our assumption that transference interpretations,closely followed by affective changes in the patient,could be useful modes of interaction was reproducible in our findings,especially when looking at the descriptive findings in the long-term data.The analysis of repetitive interaction structures demonstrated a very specific"time-lag"between therapeutic intervention and a corresponding increase in positive affect in successful therapy cases.CONCLUSION Exploring the change processes in the patients'characteristics and linking these changes to specific treatment strategies is of clinical importance when starting treatment and for its long-term progress.

International government regulatory experience of building energy-saving: A survey

Government regulation on building energy saving starts earlier in America, Holland, Japan and other developed countries, and has accumulated wealthy experience which is worth reference for China. According to practical work of building energy efficiency in China, the government regulation should to be divided into two stages: recent advance and forward continued promotion of industry. In short term, we should cultivate and improve energy efficiency market as the main task by means of enhancing awareness of energy conservation, accelerating heating system reform, implementing effective encouraging policy and setting up building energy efficiency sign system and etc. While in long term, we should put emphasis on upgrading and development of building energy efficiency industry by ways of improving standard and norm system, constructing technological guarantee system and forming socialized cooperation system and etc.

乡村治理法治体系构建问题研究

乡村治理是国家治理的基石和乡村振兴的基础。提升乡村治理和乡村建设质量,扎实有序做好乡村治理和乡村建设工作,需要重构乡村振兴中的社区治理共同体,优化社区自组织社会风险防范机制,构建新型乡村社区“三社联动”机制,构建多层次新型村庄治理共同体格局。乡村治理法治体系构建的关键在于:发挥村规民约在乡村治理中的作用,效法“枫桥经验”把矛盾纠纷化解在基层,把地方性治理规范融入全过程人民民主,给乡镇政府公共服务能力的提升增权赋能。构建乡村治理法治体系的主要路径是:发挥基层党组织在乡村治理中的引领作用,凸显基层协商民主在乡村治理中的民主意识,强化家庭家教家风在乡村治理中的修复功能,重视新乡贤在乡村振兴中的重要作用。

Doctor Song Fuyin's Experience in Treating Hashimoto's Thyroiditis Based on Qi,Blood Essence and Spirit Syndrome Differentiation Stage Classification

The paper systematically summarizes the experience of Director Song Fuyin in treating Hashimoto's thyroiditis which is a typical emotional disease that causes obvious changes in organ morphology and endocrine and body fluid metabolism.It belongs to the category of psychosomatic diseases,and is the embodiment of a series of pathogenesis processes of qi disease,blood disease,essence disease and deformation in TCM theory.The whole course of the disease is dominated by the dialectical relationship of mutual influence,mutual control,mutual root and mutual use among the deity disease,qi disease,blood disease,essence and spiritual disease.Therefore,the treatment emphasizes the dialectical relationship based on qi,blood,essence and spirit.At the beginning,the treatment focuses on regulating qi and clearing heat and eliminating phlegm;at the middle stage,the treatment focuses on replenishing essence and promoting yang and eliminating wind and calming the deity;at the late stage,the treatment focuses on replenishing qi and eliminating phlegm and promoting collages.Flexible use of calming the spirits,regulating qi,dispersing essence,eliminating phlegm,promoting blood and other methods of stage classification treatment,can be targeted,improve the cure rate of the disease.This article aims to clarify the experience of Director Song Fuyin in treating Hashimoto's thyroiditis based on syndrome differentiation of qi,blood essence and spirit.

王麟鹏教授“逐邪通络”法治疗原发性三叉神经痛

总结王麟鹏教授运用“逐邪通络”法治疗原发性三叉神经痛的学术思想。王麟鹏教授通过总结前人经验,提出“逐邪通络”针刺法,分期治疗原发性三叉神经痛;强调应根据邪气的致病特点、发作分期、正邪盛衰确定不同的针刺方案,提出局部取穴应用经络辨证为主;主张疼痛发作期以祛邪为要,缓解期主张扶正与祛邪兼而有之,重视调理脏腑、疏通经络,慎用扳机点的治疗理念,获得很好的临床效果。

聂莉芳运用调理脾胃八法治疗慢性肾脏病经验

聂莉芳教授认为慢性肾脏病的发生与脾胃功能失调密切相关,故在临床中常根据不同主症或针对不同病机灵活运用和胃降逆止呕、健脾祛湿利水、健脾益气升清、清化中焦湿热、清胃泻火通腑、脾肾气阴双补、健脾补肾温阳、肝脾同调和血八种方法治疗慢性肾脏病。聂老强调运用调理脾胃八法时要注意治脾、治胃有所侧重,主张健脾益气佐以行气消导之品以达补而不滞之效,同时兼顾脏腑之间的生克制化关系,尤其强调煎服法,认为各个诊疗细节兼顾,方能取得较好疗效。

关国华从“络气不通”辨治视网膜静脉阻塞经验探析

关国华教授临床辨治视网膜静脉阻塞思路独到。其从“络脉”角度认识视网膜血液循环系统,通过局部辨证与整体辨证结合,提出“络气不通、络脉不利”为本病基本病机,瘀血、水湿为基本病理产物,基于“络以通为用”理论,提出“和络调气”概念,灵活运用宣透、温化、活血、利湿、散结等治法遣方用药,临床常获良效。

王乐荣主任医师温督调神针刺法理论及临床应用

“温督调神”针刺法是王乐荣主任医师在30余年的中医针灸临床实践和实验研究中,学习中医学、历代及当代医家经验,探索出的中医治疗疾病的有效方法。依据督脉理论及功能,选取百会、大椎、身柱、至阳、命门、腰俞、长强等穴位,予以毫火针点刺及针刺,用以治疗神经内科、心血管、内分泌、脊柱关节、体虚易感等多学科、多系统的病证,治疗范围广泛,效果良好,值得临床推广应用。

法成令修:新《环保法》对债券定价的影响研究

本文将环境规制效应延伸到债券市场,以新《环保法》的实施作为准自然实验,采用双重差分法考察环境规制对企业债券信用利差的影响。研究发现,新《环保法》的实施能够显著提高重污染企业债券信用利差。将债券信用利差分解为流动性利差和违约利差之后发现,新《环保法》的实施显著提高了债券违约利差,对流动性利差未产生显著影响。机制检验表明,新《环保法》的实施对债券信用利差的提升作用主要通过处罚效应、投资者情绪效应以及资源效应来实现。异质性分析发现,新《环保法》的实施对债券信用利差的作用会随着客户集中度的降低、社会关注度的提高以及企业积极改善自身环境绩效而减弱。本文的研究对评估新《环保法》的经济效果,推动政策进一步精准发力,实现经济绿色转型具有一定的参考意义。

多主体协同视角下乡村民宿监管的演化博弈

乡村民宿实现了乡村旅游产业与农业、林业、服务业等产业的融合发展,有效促进了乡村振兴战略的实施。在考虑游客对乡村民宿监管作用的基础上,构建乡村民宿经营者、游客和政府监管部门三个利益主体间的演化博弈模型并通过MATLAB进行仿真模拟,是促进乡村民宿高质量发展的重要途径。通过分析,得出的主要结论为:在采用游客反映机制后,政府可以降低对乡村民宿经营者的惩罚力度;演化博弈模型有四个稳定点,其中两种情形可以实现对乡村民宿的有效监管;政府奖惩力度,游客反映对民宿声誉影响程度的提升有助于促进民宿经营者经营优质民宿;政府提高对经营者和游客的奖励不利于政府自身履行监管职能。据此,提高乡村民宿业服务质量还需要在监管方式、游客补偿、民宿经营者奖励以及声誉评价等方面予以完善。

高校化学实验教学的安全管理与实践探索

为加强实验安全管理,从安全规章制度制定、实验安全培训与实验教学内容优化,以及安全急救实训三方面构建本科生化学实验安全系统。在实验内容改进方面重点介绍了设计绿色化学实验、经典实验内容安全化及高危实验虚拟化等方法。将安全管理贯穿教学全过程,不仅有效培养学生的安全意识,而且有利于提高学生的综合素质。

刘启泉从“通调五脏安脾胃”论治尿道综合征经验

总结刘启泉教授从“通调五脏安脾胃”论治尿道综合征的临证经验。刘教授认为,尿道综合征病位在尿道,核心病机为脾胃不和,湿邪阻滞,水道不利,治疗应以“安脾胃”贯穿始终。同时,该病与五脏不调密切相关,外感、内伤、情志、饮食、劳倦及脏腑失调等均会影响五脏功能继而发病。临床中基于“通调五脏安脾胃”思想,根据患者症状表现,综合运用升清降浊以和胃通淋、养心清心以泻热通淋、益肺泻热以利湿通淋、补肾温阳以化气通淋、疏肝清肝以行气通淋的方法指导治疗,可使五脏调和,水道得利,症状缓解,淋证得瘥。

陈自明辨治月经病经验采撷

陈自明认为月经病的基本病机是冲任损伤,治疗时首先注重调理冲任、气血,用药寒温并用,偏重于温。临证时根据月经的期量色质及全身症状辨寒、热、瘀、虚,辨寒治以温经、辨热治以清经、辨瘀治以通经、辨虚治以固经。