As a kind of green additive,pectic oligosaccharide(POS) may regulate some physiological functions of animals,such as gut health,antioxidant capacity,immunity and lipid metabolism.This study aimed to identify whether P...As a kind of green additive,pectic oligosaccharide(POS) may regulate some physiological functions of animals,such as gut health,antioxidant capacity,immunity and lipid metabolism.This study aimed to identify whether POS administration can improve maternal reproduction,and to determine the possible metabolism.A total of 48 pregnant Wistar rats randomly allotted into 2 groups,and each group was fed a diet supplemented with 0 or 800 mg/kg of POS.Pectic oligosaccharide administration increased rat born number(P <0.05),did not affect rat embryo number on d 7 of gestation,but increased rat fetus number on d 14 of gestation(P <0.05).On d 14 of gestation,POS treatment improved Lactobacillus and Bifidobacterium populations and volatile fatty acid concentrations of cecal digesta(P <0.05),hormone(progesterone and nitric oxide) and cytokine(interleukin 2) concentrations of serum(P <0.05),and antioxidant capacity of serum(increased total antioxidant capacity and decreased malondialdehyde) and placenta(increased total superoxide dismutase,decreased malondialdehyde)(P <0.05) in pregnant rats.These results suggest that POS administration improved rat reproduction via decreasing fetus loss in middle gestation.This was due to the increased volatile fatty acid concentrations in rat gut improving hormone and inflammatory-cytokine productions,and antioxidant capacity.展开更多
The present study was carried out to evaluate the effects of different concentrations of pectic oligosaccharide(POS)on the physicochemical properties of Lactobacillus bulgaricus(L.bulgaricus)loaded-synbiotic hydrogel ...The present study was carried out to evaluate the effects of different concentrations of pectic oligosaccharide(POS)on the physicochemical properties of Lactobacillus bulgaricus(L.bulgaricus)loaded-synbiotic hydrogel beads and the survival of L.bulgaricus.Low methoxyl(LM)pectin-resistant starch(RS)hydrogel beads,named RS beads,were coated/filled with different concentrations of POS:0.8%(RS-POS0.8 beads),1.0%(RS-POS1.0 beads),and 1.2%(RS-POS1.2 beads).POS treatment resulted in smooth beads with less surface dent by hydrogen bonding between RS beads and POS,which was confirmed by SEM and XRD analyses.The encapsulation efficiency of RS beads and RS-POS beads ranged from 90.40%to 92.03%.In simulated gastric fluid(SGF)and bile salt solution,free L.bulgaricus completely died but L.bulgaricus encapsulated in RS-POS1.2 beads only slightly decreased.This indicates that RS-POS1.2 beads exhibited higher protective effects on L.bulgaricus under SGF condition and bile salts.Furthermore,considering the relatively higher storage stability of L.bulgaricus loaded in RS-POS1.2 beads,the present results suggest that RS beads coated/filled with POS could be used for synbiotic encapsulation,protection,and delivery of probiotics.展开更多
基金supported by the grant from the China Agricultural Research System(CARS-35)the grant from Science and Technology Support Project of Sichuan Province(2016NYZ0052 and 2016NZ0006)the fund from the research program of“Sheng Yang”students’association(B2016010)
文摘As a kind of green additive,pectic oligosaccharide(POS) may regulate some physiological functions of animals,such as gut health,antioxidant capacity,immunity and lipid metabolism.This study aimed to identify whether POS administration can improve maternal reproduction,and to determine the possible metabolism.A total of 48 pregnant Wistar rats randomly allotted into 2 groups,and each group was fed a diet supplemented with 0 or 800 mg/kg of POS.Pectic oligosaccharide administration increased rat born number(P <0.05),did not affect rat embryo number on d 7 of gestation,but increased rat fetus number on d 14 of gestation(P <0.05).On d 14 of gestation,POS treatment improved Lactobacillus and Bifidobacterium populations and volatile fatty acid concentrations of cecal digesta(P <0.05),hormone(progesterone and nitric oxide) and cytokine(interleukin 2) concentrations of serum(P <0.05),and antioxidant capacity of serum(increased total antioxidant capacity and decreased malondialdehyde) and placenta(increased total superoxide dismutase,decreased malondialdehyde)(P <0.05) in pregnant rats.These results suggest that POS administration improved rat reproduction via decreasing fetus loss in middle gestation.This was due to the increased volatile fatty acid concentrations in rat gut improving hormone and inflammatory-cytokine productions,and antioxidant capacity.
基金supported by Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(No.2018R1D1A1B07048439).
文摘The present study was carried out to evaluate the effects of different concentrations of pectic oligosaccharide(POS)on the physicochemical properties of Lactobacillus bulgaricus(L.bulgaricus)loaded-synbiotic hydrogel beads and the survival of L.bulgaricus.Low methoxyl(LM)pectin-resistant starch(RS)hydrogel beads,named RS beads,were coated/filled with different concentrations of POS:0.8%(RS-POS0.8 beads),1.0%(RS-POS1.0 beads),and 1.2%(RS-POS1.2 beads).POS treatment resulted in smooth beads with less surface dent by hydrogen bonding between RS beads and POS,which was confirmed by SEM and XRD analyses.The encapsulation efficiency of RS beads and RS-POS beads ranged from 90.40%to 92.03%.In simulated gastric fluid(SGF)and bile salt solution,free L.bulgaricus completely died but L.bulgaricus encapsulated in RS-POS1.2 beads only slightly decreased.This indicates that RS-POS1.2 beads exhibited higher protective effects on L.bulgaricus under SGF condition and bile salts.Furthermore,considering the relatively higher storage stability of L.bulgaricus loaded in RS-POS1.2 beads,the present results suggest that RS beads coated/filled with POS could be used for synbiotic encapsulation,protection,and delivery of probiotics.
