Combinatorial chemistry provides a cost-effective method for the rapid discovery of new functional peptides.One-bead one-compound(OBOC)high-throughput screening technique offers a lot of structurally diverse peptides ...Combinatorial chemistry provides a cost-effective method for the rapid discovery of new functional peptides.One-bead one-compound(OBOC)high-throughput screening technique offers a lot of structurally diverse peptides to be rapidly synthesized and screened for binding to a target of interest.The OBOC peptide library screening involves three main steps:library construction,positive beads separation,and peptide sequencing.This review mainly summarizes some special technique tips during functional peptide screening and potential future directions of the OBOC high-throughput screening technique.展开更多
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences (No.XDB36000000)the National Natural Science Foundation of China (Nos.8201101351,51890891,51890892,51890894).
文摘Combinatorial chemistry provides a cost-effective method for the rapid discovery of new functional peptides.One-bead one-compound(OBOC)high-throughput screening technique offers a lot of structurally diverse peptides to be rapidly synthesized and screened for binding to a target of interest.The OBOC peptide library screening involves three main steps:library construction,positive beads separation,and peptide sequencing.This review mainly summarizes some special technique tips during functional peptide screening and potential future directions of the OBOC high-throughput screening technique.
