The aim of this study was to evaluate the effect of the morphology of titanium implant surfaces on dental plaque biofilm formation and the antimicrobial effects of mouthrinses on dental plaque biofilms regarding these...The aim of this study was to evaluate the effect of the morphology of titanium implant surfaces on dental plaque biofilm formation and the antimicrobial effects of mouthrinses on dental plaque biofilms regarding these titanium surfaces by using an open biofilm model. The average surface roughness(RA) of three types of titanium surfaces(Smooth, hydroxyapatite(HA), sandblast large grit and acid-etching(SLA)) were tested by atomic force microscope(AFM). Subgingival plaques were collected and cultured on titanium surfaces for 4 hours to 2 weeks. After treatment with mouthrinses, characterization of dental plaque biofilms was tested by field-emission SEM(FESEM) and confocal laser scanning microscopy(CLSM). The results of AFM and SEM showed that the surface roughness and biofilm thickness of HA and SLA surfaces were significantly higher than those of smooth surface. In addition, it was revealed that the mouthrinses were effective on the killing of young dental plaque biofilms, while the more mature biofilm(14-day-old) exhibited a stronger resistance to mouthrinses used in this study. In conclusion, the roughness of titanium surfaces can affect the dental plaque biofilm formation and Colgate Plax and Listerine COOL MINT are effective mouthrinses to kill dental plaques at the early stage of biofilm growth on the titanium implant surfaces.展开更多
Objective To study its effects on reproductive performance in albino rats.Methods The chromatographic fraction （CF） of crude methanolic extract of the herb has been subcutaneously administered to female albino rats....Objective To study its effects on reproductive performance in albino rats.Methods The chromatographic fraction （CF） of crude methanolic extract of the herb has been subcutaneously administered to female albino rats. Experiments were carried out in adult cyclic females and oavriectomised （OVX） females during early gestation period. Uterine horns were collected following the respective treatment regimen to stud）, the protein profile in 15% gel SDS-PAGE.Results The CF induced changes in the expression of protein in rat uterus. New proteins have been expressed in uterus of adult cyclic females having ovary in-situ. The OVX females treated with CF showed altered uterine protein profile compared with that of OVX control and OVX estradiol-17β （E2） treated rats. The CF exerted its effect on expression of uterine protein during early gestation period in rats. While uterine proteins of CF treated females were similar to that of controls during preimplantation period; many of the proteins on day 6 of gestation have been found either missing or expressed in lesser intensity.Conclusion The root of Polygonum hydropiper contains potential compound（s） which can alter the reproductive performance of female rats modulating uterine protein expression.展开更多
基金Funde by the National Natural Science Foundation of China(81371170)
文摘The aim of this study was to evaluate the effect of the morphology of titanium implant surfaces on dental plaque biofilm formation and the antimicrobial effects of mouthrinses on dental plaque biofilms regarding these titanium surfaces by using an open biofilm model. The average surface roughness(RA) of three types of titanium surfaces(Smooth, hydroxyapatite(HA), sandblast large grit and acid-etching(SLA)) were tested by atomic force microscope(AFM). Subgingival plaques were collected and cultured on titanium surfaces for 4 hours to 2 weeks. After treatment with mouthrinses, characterization of dental plaque biofilms was tested by field-emission SEM(FESEM) and confocal laser scanning microscopy(CLSM). The results of AFM and SEM showed that the surface roughness and biofilm thickness of HA and SLA surfaces were significantly higher than those of smooth surface. In addition, it was revealed that the mouthrinses were effective on the killing of young dental plaque biofilms, while the more mature biofilm(14-day-old) exhibited a stronger resistance to mouthrinses used in this study. In conclusion, the roughness of titanium surfaces can affect the dental plaque biofilm formation and Colgate Plax and Listerine COOL MINT are effective mouthrinses to kill dental plaques at the early stage of biofilm growth on the titanium implant surfaces.
基金support and the Department of Zoology,Rajiv Gandhi University for providing laboratory facilities for completion of the work
文摘Objective To study its effects on reproductive performance in albino rats.Methods The chromatographic fraction （CF） of crude methanolic extract of the herb has been subcutaneously administered to female albino rats. Experiments were carried out in adult cyclic females and oavriectomised （OVX） females during early gestation period. Uterine horns were collected following the respective treatment regimen to stud）, the protein profile in 15% gel SDS-PAGE.Results The CF induced changes in the expression of protein in rat uterus. New proteins have been expressed in uterus of adult cyclic females having ovary in-situ. The OVX females treated with CF showed altered uterine protein profile compared with that of OVX control and OVX estradiol-17β （E2） treated rats. The CF exerted its effect on expression of uterine protein during early gestation period in rats. While uterine proteins of CF treated females were similar to that of controls during preimplantation period; many of the proteins on day 6 of gestation have been found either missing or expressed in lesser intensity.Conclusion The root of Polygonum hydropiper contains potential compound（s） which can alter the reproductive performance of female rats modulating uterine protein expression.
