TWENTY one-year-old Fan Chunmei,a singer from an artensemble of the Chinese People‘s Liberation Army,won firstprize in the fourth Sound of Asia International Popular SongCompetition,held in Kazakhstan on July 19.Ai J...TWENTY one-year-old Fan Chunmei,a singer from an artensemble of the Chinese People‘s Liberation Army,won firstprize in the fourth Sound of Asia International Popular SongCompetition,held in Kazakhstan on July 19.Ai Jing,from LiaoningProvince,won the Alma-Ata Mayor’s Prize.She sang“My 1997.”Yuan Chenyie of the China Opera Theater took second prize inthe Classical division of the Tokyo International Vocal Music Com-petition,held in Tokyo in June this year.展开更多
[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PP...[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.展开更多
Pourfour du petit综合征系因交感神经传导径路中,自下丘脑交感神经中枢往下至支配头颈上肢段任一部位刺激性病损所致。其临床表现与Horner征恰恰相反,表现为病变侧瞳孔散大、睑裂开大、眼球突出、头颈上肢或半身皮肤血管收缩以及出汗...Pourfour du petit综合征系因交感神经传导径路中,自下丘脑交感神经中枢往下至支配头颈上肢段任一部位刺激性病损所致。其临床表现与Horner征恰恰相反,表现为病变侧瞳孔散大、睑裂开大、眼球突出、头颈上肢或半身皮肤血管收缩以及出汗增多。展开更多
Peste ties petits ruminants is a kind of acute eontagious disease infecting goats anti sheep. In this study, antibtly monitoring and tracking of healthy goat and sheep immunized by peste des petits ruminants vaccine i...Peste ties petits ruminants is a kind of acute eontagious disease infecting goats anti sheep. In this study, antibtly monitoring and tracking of healthy goat and sheep immunized by peste des petits ruminants vaccine in Changping District of Beijing City were conducted, aiming at providing a reference for the devel- opment of effective immunization procedures.展开更多
近日,2016年度睡莲新品种比赛（N ew W aterlily C om petition）获奖名单出炉：‘舞会皇后’及‘少女’获耐寒睡莲类冠、亚军;‘维吉尼亚·麦克莱恩’及‘星系’获热带睡莲类冠、亚军;‘侦探艾丽卡’及‘玛蒂妮娜’获亚属间杂交种...近日,2016年度睡莲新品种比赛（N ew W aterlily C om petition）获奖名单出炉：‘舞会皇后’及‘少女’获耐寒睡莲类冠、亚军;‘维吉尼亚·麦克莱恩’及‘星系’获热带睡莲类冠、亚军;‘侦探艾丽卡’及‘玛蒂妮娜’获亚属间杂交种睡莲类冠、亚军,另外,‘侦探艾丽卡’及‘维吉尼亚·麦克莱恩’获年度最佳新品种睡莲冠、亚军（图1）。笔者育成的睡莲新品种‘侦探艾丽卡’在此比赛中成为年度双料冠军,并在国际睡莲与水景园协会获得了国际权威登录。展开更多
In this paper, the existence of strictly positive solutions for N-species nonau- tonom ous Kolm ogorov com petition system s is studied. By applying the Schauder's fixed point theorem som e new sufficient condit...In this paper, the existence of strictly positive solutions for N-species nonau- tonom ous Kolm ogorov com petition system s is studied. By applying the Schauder's fixed point theorem som e new sufficient conditions are established. In particular, for the alm ost periodic system , the existence of strictly positive alm ostperiodic solutions is obtained. Som e previous results are im proved and generalized.展开更多
Peste des petits ruminant (PPR) is a contagious disease of small ruminants caused by a virus that belongs to the genus Morbillivirus of the family Paramyxoviridae. This study aimed to determine the seroprevalence of P...Peste des petits ruminant (PPR) is a contagious disease of small ruminants caused by a virus that belongs to the genus Morbillivirus of the family Paramyxoviridae. This study aimed to determine the seroprevalence of PPR disease in sheep and goats and its associated risk factors in Kassala State, Eastern Sudan. Across sectional study was conducted during the period from 30th August to 25th November 2015. The study was carried out using a structured questionnaire survey and a total of 918 blood samples were collected from apparently healthy unvaccinated sheep and goats in different localities in State of Kassala. A total of 546 sheep and 372 goats were tested for specific antibodies to nucleoprotein (NP) by competitive enzyme linked immunosorbent assay (cELISA). The apparent overall prevalence of PPR antibodies in Kassala was 58.2% while the true prevalence was calculated to be 61.3%. The apparent prevalence in sheep and goats was 68.1% and 43.5% respectively. Univariate analysis showed that the risk factors had significant associations with a cELISA positive status: locality, species, age, breed, husbandry system, housing mode, animals movement (p = 0.000) and animals sharing pasture and water (p = 0.003), while sex and newly introduced animals were not significant risk factors (p = 0.771) (p = 0.050) respectively. Factors found that significantly associated (p < 0.05) with increased odds of being cELISA positive in multivariate analysis were localities, species, age and newly introduced animals. The prevalence differed between localities and was the highest in the River Atbara (84.0%) locality, whereas it was lowest in Delta North (29.0%). No significant difference was observed among the sexes. However, the prevalence differed in different age groups and was 52.25% in animals of less than six months old;49.3% were between seven months and two years old and 65.5% were above two years old. In different husbandry systems, the prevalence was 47.9%, 73.0% and 49.2% in intensive, open grazing and pastoral systems respectively. Housing type effects were also observed;the highest prevalence was in animals housed in metal fence (83.3%). The movement pattern showed significant effect, where the prevalence was the highest (81.3%) in animals that move inter-states/inter-localities. It is concluded that the disease is endemic in Kassala State, high prevalent in sheep and goats, posing a threat to animal exportation, and may have a serious economic influence. Owners and herders should compulsorily vaccinate their animals yearly and animals should be investigated periodically for implementation of crucial eradication program.展开更多
文摘TWENTY one-year-old Fan Chunmei,a singer from an artensemble of the Chinese People‘s Liberation Army,won firstprize in the fourth Sound of Asia International Popular SongCompetition,held in Kazakhstan on July 19.Ai Jing,from LiaoningProvince,won the Alma-Ata Mayor’s Prize.She sang“My 1997.”Yuan Chenyie of the China Opera Theater took second prize inthe Classical division of the Tokyo International Vocal Music Com-petition,held in Tokyo in June this year.
基金Supported by National Key R&D Program for the Prevention and Control of Major Exotic Animal Diseases(2022YFD1800500)National Mutton Sheep Industrial Technology System(CARS39)+2 种基金Key Research and Development Program of Shandong Province(Major Science and Technology Innovation Project)(2021CXGC011306)Scientific Research Project of General Administration of Customs(2024HK033)Scientific Research Project of Jinan Customs(2023JK005).
文摘[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.
文摘Peste ties petits ruminants is a kind of acute eontagious disease infecting goats anti sheep. In this study, antibtly monitoring and tracking of healthy goat and sheep immunized by peste des petits ruminants vaccine in Changping District of Beijing City were conducted, aiming at providing a reference for the devel- opment of effective immunization procedures.
文摘近日,2016年度睡莲新品种比赛（N ew W aterlily C om petition）获奖名单出炉：‘舞会皇后’及‘少女’获耐寒睡莲类冠、亚军;‘维吉尼亚·麦克莱恩’及‘星系’获热带睡莲类冠、亚军;‘侦探艾丽卡’及‘玛蒂妮娜’获亚属间杂交种睡莲类冠、亚军,另外,‘侦探艾丽卡’及‘维吉尼亚·麦克莱恩’获年度最佳新品种睡莲冠、亚军（图1）。笔者育成的睡莲新品种‘侦探艾丽卡’在此比赛中成为年度双料冠军,并在国际睡莲与水景园协会获得了国际权威登录。
文摘In this paper, the existence of strictly positive solutions for N-species nonau- tonom ous Kolm ogorov com petition system s is studied. By applying the Schauder's fixed point theorem som e new sufficient conditions are established. In particular, for the alm ost periodic system , the existence of strictly positive alm ostperiodic solutions is obtained. Som e previous results are im proved and generalized.
文摘Peste des petits ruminant (PPR) is a contagious disease of small ruminants caused by a virus that belongs to the genus Morbillivirus of the family Paramyxoviridae. This study aimed to determine the seroprevalence of PPR disease in sheep and goats and its associated risk factors in Kassala State, Eastern Sudan. Across sectional study was conducted during the period from 30th August to 25th November 2015. The study was carried out using a structured questionnaire survey and a total of 918 blood samples were collected from apparently healthy unvaccinated sheep and goats in different localities in State of Kassala. A total of 546 sheep and 372 goats were tested for specific antibodies to nucleoprotein (NP) by competitive enzyme linked immunosorbent assay (cELISA). The apparent overall prevalence of PPR antibodies in Kassala was 58.2% while the true prevalence was calculated to be 61.3%. The apparent prevalence in sheep and goats was 68.1% and 43.5% respectively. Univariate analysis showed that the risk factors had significant associations with a cELISA positive status: locality, species, age, breed, husbandry system, housing mode, animals movement (p = 0.000) and animals sharing pasture and water (p = 0.003), while sex and newly introduced animals were not significant risk factors (p = 0.771) (p = 0.050) respectively. Factors found that significantly associated (p < 0.05) with increased odds of being cELISA positive in multivariate analysis were localities, species, age and newly introduced animals. The prevalence differed between localities and was the highest in the River Atbara (84.0%) locality, whereas it was lowest in Delta North (29.0%). No significant difference was observed among the sexes. However, the prevalence differed in different age groups and was 52.25% in animals of less than six months old;49.3% were between seven months and two years old and 65.5% were above two years old. In different husbandry systems, the prevalence was 47.9%, 73.0% and 49.2% in intensive, open grazing and pastoral systems respectively. Housing type effects were also observed;the highest prevalence was in animals housed in metal fence (83.3%). The movement pattern showed significant effect, where the prevalence was the highest (81.3%) in animals that move inter-states/inter-localities. It is concluded that the disease is endemic in Kassala State, high prevalent in sheep and goats, posing a threat to animal exportation, and may have a serious economic influence. Owners and herders should compulsorily vaccinate their animals yearly and animals should be investigated periodically for implementation of crucial eradication program.
