Phosphorus fractionation and bio-availability in Taihu Lake(China) sediments

Typical sediments from Taihu Lake, a meso-to-hypereutrophic lake, were collected and examined on the basis of P-fractionation by sequential extraction scheme. Sedimentary inorganic phosphorus were fractioned into four forms and the rank order according to the mean concentration of P-fractions in Taihu Lake was NaOH-P>BD-P>HCl-P>NH_4Cl-P. The concentrations of BD-P were linearly correlated with the content of active Fe(R2=0.96). Also, the linear relationship between the sum of BD-P and NaOH-P and the sum of active Fe and active Al content was observed within the six sediments investigated(R2=0.96). Moreover, the bio-available phosphorus(BAP) content was estimated by the sum of NH_4Cl-P, BD-P, and NaOH-P, viz. BAP=NH_4Cl-P+NaOH-P+BD-P. In Taihu Lake, the BAP contents are ranging from 0.10 mg/g dw to 1.25 mg/g dw, and average 0.40 mg/g dw for all sediment samples. The relative contributions of BAP to total sedimentary phosphorus(TP) and inorganic sedimentary phosphorus(IP) range from 18.67% to 50.79%(33.61% on average) and from 52.82% to 82.09%(67.81% on average), respectively.

Characterizing Constituents of Sediment Phosphorus Fractionation in a Freshwater Shallow Lake System

This study aimed to characterize constituents of phosphorus （P） fractionation as wel as reciprocities among factors in sediments of a freshwater shal ow lake. [Method] Surface sediment was discretely sampled at 24 sites through Van Veen grabs. Based on a modified sequential extraction scheme, P fractionation was determined as Fe/Al bound P （Fe/Al-P）, Ca bound P （Ca-P）, solute and re-ductive P （S/R-P） and organic P （OP）. Curve estimation and Pearson product-mo-ment correlation were employed for statistical analysis. [Result] Total P （TP） content ranged from 443 to 774 mg/kg. Inorganic P （IP） was the major component of TP, of which Ca-P was dominated with an average of 51%±9.7%. Average contents of P fractionation were in the fol owing order： Ca-P（51%） 〉 OP（29%） 〉 S/R-P（8%） 〉Fe/Al-P（7%）. The molar ratio of Fe to P was 11 - 20, close to the threshold value of P leaching. [Conclusion] In freshwater shal ow lakes, IP and Ca-P were prone to be relatively high, whereas Fe/Al-P was low compared with deep lakes. Occurrence of spatial y monotonic gradient indicated the primary causation of anthropogenic sources. Imminent sediment P liberation was also expected. Close associations a-mong TP, Fe/Al-P and Ca-P, implying that anthropogenic P was mainly bound to metals in particulates. Significant correlations of TOC and P fractionation highlighted endogenous mechanism and authigenic origin in sediments.

Chemical Fractionation and Sorption of Phosphorus in Greek Inceptisols

Phosphorus fractionation and sorption, both separately and jointly, were studied in two cultivated Inceptisols in Northern Greece. Hedley＇s extraction was used to separate soil phosphorus （P） into inorganic Pi （geochemical） and organic Po （biological） fractions. Direct extractable P by Olsen （Olsen-P）, by Mehlich llI （M3-P） and by ammonium oxalate （Pox） was also determined. Phosphorus sorption was carried out with 1：10 soil/solution ratio and sorption parameters were derived from the Langmuir model to the experimental data. Most of the total P occurred in inorganic P forms （74% of Pt）, while organic P comprised only 26% of the Pt. Among the various inorganic P forms relatively large amount of residual P （111 mg kg^-1） was observed, while occluded P in calcium phosphate minerals （d.HCI-Pi） and in Fe, Al-oxides （c.HCI-Pi） existed in equal amounts （83.1 and 83.7 mg kg^-1 respectively）. The phosphorus sorption parameters showed positive relationships with clay content, cation exchange capacity and the sum of exchangeable calcium plus magnesium. Overall, this study indicated that Ca and Mg compounds strongly influence the P chemistry in moderately weathered soils, with relatively high concentration of primary P minerals.

Effects of phosphorus fertilizer application rate on transformation processes of phosphorus fractions in the purple alluvial soil of a riparian zone

Effects of phosphorus(P)fertilizer application rate on soil transformation processes of P fraction are still unclear in the riparian zone.Purple alluvial soils in the riparian zone of the Three Gorges Reservoir were collected to conduct a 21-day incubation executed by two hydrological environments(drying and flooding)and ten application rates of P fertilizer.Transformation percentages of P fertilizer(TPPF)were calculated as content differences of soil P fractions between fertilizer addition and none fertilizer addition divided by soil total P increases caused by fertilizer addition.TPPF to inorganic P extracted by sodium hydroxide(NaOH-Pi)and hydrochloric acid(HCl-Pi)increase by 20.91%(9.71%)and 24.26%(40.72%)under the drying(flooding)environment.Instead,TPPF to the other fractions decrease.Phosphorus fertilizer input mainly has indirect positive and negative effects on organic P via precipitated P under the drying and flooding environments and finally has indirect positive effects on labile P(p<0.001).Percentage changes of water-soluble inorganic P(H2O-Pi)and HCl-Pi under the flooding environment are higher than that under the drying environment,and percentage changes of organic P extracted by sodium hydrogen carbonate(Na HCO3-Po)and NaOH-Pi show an opposite trend(p<0.01).?(differences in soil P fraction content between flooding and drying incubations)H2O-Pi is negatively correlated with?NaHCO3-Po,and?NaHCO3-Po is positively correlated with?NaOH-Pi(p<0.001).In conclusion,P fertilizer is transformed more into precipitated P than into other P fractions with an application rate increase.Phosphorus fertilizer input mainly increases organic P via precipitated P under the drying environment and decreases organic P via precipitated P under the flooding environment,and organic P is further transformed into labile P.With P fertilizer input,P release caused by flooding is derived from NaHCO3-Po release triggered by NaOH-Pi release.The results can be helpful for the understanding of P fertilizer migration processes from the riparian zone soil to the Three Gorges Reservoir under rain leaching and flooding.

Effects of Biochar and Wood Vinegar on Labile Phosphorus Pool in Soda Saline-Alkaline Soil

A pot experiment was conducted to research the effect of biochar and wood vinegar on labile phosphorus fractions in saline-alkali soil.There were eight treatments,including CK(0 kg•hm-2 biochar+0 kg•hm-2 wood vinegar),C1(0.6 t•hm-2 biochar),C2(0.6 t•hm-2 wood vinegar),C3(1.2 t•hm-2 wood vinegar),C4(1.8 t•hm-2 wood vinegar),C5(0.6 t•hm-2 biochar+0.6 t•hm-2 wood vinegar),C6(0.6 t•hm-2 biochar+1.2 t•hm-2 wood vinegar),and C7(0.6 t•hm-2 biochar+1.8 t•hm-2 wood vinegar).The results showed that biochar without wood vinegar and the co-application of biochar and wood vinegar significantly increased soil total phosphorus content.Meanwhile,compared with CK,all of treatments increased resin phosphorus and sodium bicarbonate-extracted inorganic phosphorus(NaHCO3-Pi)contents in saline-alkali soil.Especially,the contents of resin phosphorus and NaHCO3-Pi under C5,C6,and C7 treatments were higher than those of C2,C3,and C4 treatments,respectively,indicating that the increases of labile phosphorus contents under the co-application of biochar and wood vinegar were better than those of the alone application of biochar and wood vinegar.Each treatment increased the proportion of labile phosphorus pool in saline-alkali soil and the proportion of labile phosphorus pool increased with the increase of the amount of wood vinegar.In addition,the application of biochar and wood vinegar increased the 100-grain weight of rice,and C6 treatment had the best effect,increasing the 100-grain weight by 134.35%.Therefore,the application of biochar and wood vinegar in saline-alkali soil could improve the soil phosphorus availability,increase the weight of rice grains,thereby realizing the resource utilization of agricultural waste and the sustainable development of agriculture.

Distribution of Phosphorus in Minjingu Phosphate Rock and Ikutha Rock Samples

Minjingu Phosphate Rock （MPR） from Northern Tanzania and the Ikutha Phosphate Rock （IPR） found in Central-Southeast Kenya are well documented as potential sources of phosphorous （P） available in East Africa. On-farm trials in phosphate-deficient soils in Western Kenya demonstrated MPR to be as effective as triple superphosphate （TSP） - 20% P, at equal P rates. The aim of this work is to determine the distribution of phosphorus in these phosphate rocks （PRs）. The different phosphorus fractions were extracted using the modified Williams extraction procedure and analysis carried on a UV/VIS spectrometer （SHIMADZU UV-220-02 and NOVASPEC II）. The analysis showed that the most abundant form of phosphorus in the phosphate rocks was the Inorganic Phosphorus （IP） contributing 74.20% of total phosphorus （TP） for Minjingu, and 83,28% of total phosphorus for Ikutha phosphate rock.

Phosphorus fractions and its release in the sediments of Haihe River,China

The amounts and forms of phosphorus （P） in surface sediments of Haihe River, Tianjin, North China, were examined using a sequential chemical extraction procedure. Five fractions of sedimentary P, including loosely sorbed P （NH4Cl-P）, redox-sensitive P （BD-P）, metal oxide bound P （NaOH-P）, calcium bound P （HCI-P）, and residual P （Res-P） （organic and refractory P）, were separately quantified. The results indicated that the contents of different P fractions in the sediments varied greatly. The total P （TP） contents ranged from 968 to 2017 mg/kg. Phosphorus contents in NH4Cl-P, BD-P, NaOH-P, and HCl-P ranged from 6.7 to 26.6 mg/kg, 54.5 to 90.2 mg/kg, 185.2 to 382.5 mg/kg, and 252.3 to 425.5 mg/kg, respectively, which represented 1.2%-3.2%, 7.7%-13%, 33.3%-48.9%, and 36.2%-54.2% of the sedimentary inorganic P, respectively. For all the sediment samples, the rank order of P-fractions was Res-P 〉 HCl-P 〉 NaOH-P 〉 BD-P 〉 NH4Cl-P. The highly positive relationship between the amounts of P released from the sediments and those in the NH4Cl-P and BD-P fractions, indicated that NH4Cl-P and BD-P were the main fractions that can release P easily.

Impact of Long-Term Fertilization on Phosphorus Status in Black Soil

A twelve-year field trial was carried out to determine the impact of long-term fertilization on phosphorus (P) distribu- tion in the soil profile, P balance, and the transformation and availability of soil P in a black soil (Phaeozem, FAO). Under a wheat-soybean-corn rotation, during twelve years where no fertilizer was applied, crops removed 60 and 81 mg P kg-1 soil in the control and NK treatment, respectively. About one third of the P absorbed by crops was originated from organic P. Ca2-P, Cag-P, Al-P and Fe-P were the main forms of inorganic P absorbed by crops. The surplus P from fertilization remained in the 0-20 cm soil layer and increased with the application rate of P. The combined application of NP or NPK increased available P to a lesser extent than the combined application of PK. Fertilizer P had mainly transformed to the Al-P, Fe-P, Ca2-P, and Cas-P forms. By using the P budget (X), changes in total P (Y1) and available P (Y2) in soils under the current cropping system could be predicted by the equations: Y1 = 0.02 + 1.01X and Y2 = 2.08 + 0.15X.

Changes in Phosphorus Fractions, pH, and Phosphatase Activity in Rhizosphere of Two Rice Genotypes

A rhizobox experiment with two phosphorus (P) treatments, zero-P (0 mg P kg-1) and plus-P (100 mg P kg-1) as Ca(H2PO4)2·H2O, was conducted to study the chemical and biochemical properties in the rhizosphere of two rice genotypes (cv. Zhongbu 51 and Pembe) different in P uptake ability and their relationship with the depletion of soil P fractions. Plant P uptake, pH, phosphatase activity, and soil P fractions in the rhizosphere were measured. Both total dry weight and total P uptake of Pembe were significantly (P < 0.05) higher than those of Zhongbu 51 in the zero-P and plus-P treatments. Significant depletions of resin-Pi, NaHCO3-Pi, NaHCO3-Po, and NaOH-Pi, where Pi stands for inorganic P and Po for organic P, were observed in the rhizosphere of both Zhongbu 51 and Pembe under both P treatments. Pembe showed a greater ability than Zhongbu 51 in depleting resin-Pi, NaHCO3-Pi, NaHCO3-Po, NaOH-Pi, and NaOH- Po in the rhizosphere. HCl-Pi and residual-P were not depleted in the rhizosphere of both genotypes, regardless of P treatments despite significant acidification in the rhizosphere of Pembe under zero-P treatment. Higher acid phosphatase (AcPME) activity and alkaline phosphatase (AlPME) activity were observed in the rhizosphere of both Zhongbu 51 and Pembe compared to the corresponding controls without plant. AcPME activity was negatively (P < 0.01) correlated to NaHCO3-Po concentration in the rhizosphere of both Zhongbu 51 and Pembe, suggesting that AcPME was associated with the mineralization of soil organic P.

Organic Phosphorus in Shallow Lake Sediments in Middle and Lower Reaches of the Yangtze River Area in China

Thirteen sediment core samples(0-10 cm) were taken from the seven lakes in the middle and lower reaches of the Yangtze River to determine the contents and distributions of organic phosphorus(P) fractions in the sediments of the shallow lakes in the area.The organic P fractions in the sediments were in the order of moderately labile organic P(MLOP) > moderately resistant organic P(MROP) > highly resistant organic P(HROP) > labile organic P(LOP),with average proportional ratios of 13.2:2.8:1.3:1.0.LOP,MLOP,and MROP were significantly related to the contents of total organic carbon(TOC),water-soluble P(WSP),algal-available P(AAP),NaHCO3-extractable P(Olsen-P),total P(TP),organic P(OP),and inorganic P(IP).However,HROP was significantly related to OP and weakly correlated with TOC,WSP,AAP,Olsen-P,TP or IP.This suggested that organic P,especially LOP and MLOP in sediments,deserved even greater attention than IP in regards to lake eutrophication.In terms of organic P,sediments were more hazardous than soils in lake eutrophication.Although OP concentrations were higher in moderately polluted sediment than those in heavily polluted sediment,LOP and MLOP were higher in the heavily polluted sediment,which indicated that heavily polluted sediment was more hazardous than moderately polluted sediment in lake eutrophication.

Interaction of soil microbial communities and phosphorus fractions under long-term fertilization in paddy soil

Understanding the impact of biological activities on the soil phosphorus(P)distribution under long-term fertilizer application can facilitate better soil P fertility management.Therefore,the primary objectives of this study were to investigate the effect of long-term(since 1981)fertilizer application on the soil P fractions and microbial community and to evaluate correlations between the microbial community structure and P distribution.The following treatments were implemented in a long-term field trial:no fertilization(CK),inorganic N and K(NK),inorganic P and K(PK),inorganic N,P and K(NPK)and manure+NPK(MNPK)fertilization.The study showed that the soil pH,soil organic carbon and total and available N and P concentrations were considerably higher in the MNPK treatment than in the CK treatment.The soil microbial biomass C,N and P concentrations were also significantly higher in the MNPK treatment than in the CK treatment.Among fertilization treatments,theβ-1,4-glucosidase,α-1,4-glucosidase,urease,acid phosphatase and phosphodiesterase activities were the highest in the MNPK treatment.Compared to inorganic fertilization,the MNPK treatment increased the labile soil P fractions and decreased the residual soil P concentration.Continuous fertilization significantly affected the soil microbial composition.The total phospholipid fatty acid(PLFA)concentrations in the NK,PK,NPK and MNPK treatments were 23.3,43.1,48.7 and 87.7%higher,respectively,than in the CK treatment.A significant correlation was observed between the microbial community and soil P fractions.Moreover,the aggregated boosted tree(ABT)model showed that among the various soil biochemical properties,the total PLFA concentration was the factor that most influenced the active P pool,accounting for 35.4%of the relative influence of all soil biochemical properties examined.These findings reveal that combined manure and inorganic fertilizer application is a better approach than applying inorganic fertilizer alone for sustaining long-term P fertility by mediating soil biological activity.

Effect of long-term fertilization on phosphorus fractions in different soil layers and their quantitative relationships with soil properties

Investigating the dynamics and distribution of soil phosphorus(P) fractions can provide a basis for enhancing P utilization by crops. Four treatments from a 29-year long-term experiment in black soil with maize cropping were involved in this study: no fertilizer(CK), inorganic nitrogen and potassium(NK), inorganic nitrogen, phosphorus, and potassium(NPK), and NPK plus manure(NPKM). We analyzed soil P fractions in different soil layers using a modified Hedley sequential method. The long-term NPKM treatment significantly increased total P by 0.6–1.6 times in the different soil layers. The Olsen-P concentration far exceeded the environmental threshold for soil Olsen-P(50.6 mg kg) in the NPKM treatment in the 0–60 cm soil profile. Moreover, the concentrations and proportion of labile and partially labile inorganic P(Pi) fractions(i.e., Na HCO-extracted Pi, Na OH-extracted Pi, and dilute HClextracted Pi) to the sum of all P fractions(Pt) in the 0–60 cm soil profile were higher in the NPKM treatment than in the NPK treatment, indicating that manure could promote the transformation of non-labile into more labile forms of P in soil, possibly by manure reducing P fixation by soil particles. Soil organic matter, Mehlich-3 extractable iron(Fe), and organic-bound aluminum were increased by fertilization, and were the main factors influencing the differences in the P fractions in the 0–20 cm soil layer. Soil mineral components, i.e., free Fe oxide and Ca CO, were the main factors influencing the P fractions in the subsoil. The soil P transformation process varied with soil layer and fertilization. Application of manure fertilizer can increase the labile(Olsen) P concentrations of the various soil layers, and thus should reduce the mineral P fertilizer requirement for crop growth and reduce potential environmental damage.

Characteristics of inorganic phosphorus fractions and their correlations with soil properties in three non-acidic soils

Understanding the characteristics and influences of various factors on phosphorus(P)fractions is of significance for promoting the efficiency of soil P.Based on long-term experiments on black soil,fluvo-aquic soil,and loess soil,which belong to Phaeozems,Cambisols,and Anthrosols in the World Reference Base for Soil Resources(WRB),respectively,five fertilization practices were selected and divided into three groups:no P fertilizer(CK/NK),balanced fertilizer(NPK/NPKS),and manure plus mineral fertilizer(NPKM).Soil inorganic P(Pi)fractions and soil properties were analyzed to investigate the characteristics of the Pi fractions and the relationships between Pi fractions and various soil properties.The results showed that the proportion of Ca_(10)-P in the sum of total Pi fractions was the highest in the three soils,accounting for 33.5%in black soil,48.8%in fluvo-aquic soil,and 44.8%in loess soil.Long-term fertilization practices resulted in periodic changes in soil Pi accumulation or depletion.For black soil and fluvo-aquic soil,the Pi accumulation was higher in the late period(10–20 years)of fertilization than in the early period(0–10 years)under NPK/NPKS and NPKM,whereas the opposite result was found in loess soil.The Pi accumulation occurred in all Pi fractions in black soil;mainly in Ca_(8)-P,Fe-P,and Ca_(10)-P in fluvo-aquic soil;and in Ca_(2)-P,Ca_(8)-P,and O-P in loess soil.Under CK/NK,the soil Pi was depleted mainly in the early period in each of the three soils.In addition to the labile Pi(Ca_(2)-P)and moderately labile Pi(Ca_(8)-P,Fe-P,Al-P),the Ca_(10)-P in black soil and fluvo-aquic soil and O-P in loess soil could also be used by crops.Redundancy analysis showed that soil properties explained more than 90%of the variation in the Pi fractions in each soil,and the explanatory percentages of soil organic matter(SOM)were 43.6%in black soil,74.6%in fluvo-aquic,and 38.2%in loess soil.Consequently,decisions regarding the application of P fertilizer should consider the accumulation rate and the variations in Pi fractions driven by soil properties in non-acidic soils.

Dynamics of soil inorganic phosphorus fractions at aggregate scales in a chronosequence of Chinese fir plantations

Successive cultivation of Chinese fir(Cunninghamia lanceolata) would markedly affect the distribution and accumulation of soil inorganic phosphorus(Pi).However,how different chronosequence phases of Chinese fir plantations exerting influences on the quality and quantity of soil Pi fractions in aggregate-scale remain poorly understood. This study researched the dynamic changes of aggregate-related Pi fractions encompassing occluded-P(O-P), aluminum-bound P(Al-P), iron-bound P(Fe-P), and calcium-bound P(Ca-P) in topsoil(0-20 cm) from different stand aged(9-, 17-, and 26-yr) Chinese fir plantations and one nearby abandoned land(CK) in Rongshui County,Guangxi, China. In this study, soil aggregates were classified into micro-aggregates(< 0.25 mm), small macro-aggregates(1-0.25 mm), medium macroaggregates(2-1 mm), and large macro-aggregates(> 2 mm) by one wet-sieving process. As the primary aggregate fractions correlated with better soil aggregate stability, the large macro-aggregates took the highest proportion in all aggregate sizes regardless of various stand ages of Chinese fir plantations. Besides, the 17-yr plantations of Chinese fir displayed the highest stability of aggregates structure. Compared with CK, all four soil Pi fractions from three different stand ages of Chinese fir plantations generally showed increasing trends.Irrespective of chronosequence phases, Al-P was mainly carried by small macro-aggregates. O-P showed the opposite tendency to Al-P, which had the lowest content in small macro-aggregates. Fe-P and Ca-P showed an even distribution in all aggregates.The contribution rates and stocks of each Pi fraction exhibited close relevance to the content of soil aggregates. As revealed from the results, planting of Chinese fir before 17-yr was beneficial to prompt the formation of large macro-aggregates and the level of soil P. But after 17-yr, successive monoculture planting of Chinese fir would reduce the stability of soil aggregates and render the losses of soil P. The dynamics of soil total phosphorous(TP) and Pi fractions contents were highly related to the stand ages of Chinese fir plantations, but less related to the distribution of soil aggregate sizes. As the major carriers for soil P stocks, the large macro-aggregates played a vital role in the cycles and reserves of soil P.

Phosphorus accumulation and eutrophication in feed-supply freshwater fishponds

The rapid growth and intensification of freshwater fishery can cause imbalances between phosphorus （P） input in feed and its output in produce. This aquaculture can result in enriching exogenous P in fishponds and, consequently, accelerates the process of eutrophication. To assess relations among input, accumulation, release of P and as a consequence degrading water quality in terms of chlorophyll-a （Chl-a） in freshwater fishponds, fourteen fishponds with feed supply, nine fishponds without feed supply, and five nonfish ponds in Shaoxing Plain, southeast China were selected for comparing P accumulation in their waters and surface sediments. Surface sediment samples were collected from each pond to evaluate their total P, water soluble P, Olsen P, algal available P, and P fractions. Water samples were also collected from the ponds to measure concentrations of dissolved P and Chl-a. Total P in the sediments ranged from 0.88 to 1.73 g/kg in the fishponds with feed supply, that in the non-fish ponds ranged from 0.47 to 0.86 g/kg. Organic P, accounted for 23% to 60% of total P in the sediments, was an important P fraction and increased linearly with increasing organic matter. Long-term application of feeds resulted in increased P availability in the bottom sediments and degradation of water quality in the freshwater fishponds. Compared with non-fish ponds, sediments from the feed-supplied fishponds contained considerably higher Olsen P, algal available P, and water soluble P. Higher proportions of the labile P （NH4Cl-P） and potentially labile P （NaOH-IP） were also found in the sediments from the fishponds. High solubility of P in the sediments resulted in elevation of P and chlorophyll-a concentration in the pond water. The dissolved P concentration in the pond water increased in the order of non-fish ponds （12μg/L） 〈 fishponds without feed supply （24 μg/L） 〈 fishponds with feed supply （66 μg/L）. Linear correlations between concentrations of total P, Olsen-P, algal available P, water-soluble P and P concentration in saturation extracts in the sediments and dissolved P in the pond water indicated that there was a buffering action of the sediment constituents on the dissolved P.

Soil phosphorus fractions and their availability over natural succession from clear-cut of a mixed broadleaved and Korean pine forest in northeast China

To assess phosphorus(P)status of forest soil under naturally restored vegetation,P fractions in the 10-cm soil layer were quantified at different successional stages on the clear-cut site of mixed broadleaved and Korean pine forest.Four communities of shrub,softwood broad-leaved forest,softwood and hardwood broad-leaved forest,and hardwood broad-leaved forest represented different successional stages.A soil sample from a primary broad-leaved and Korean pine stand was the control.A sequential P fractionation scheme extracted empirically defined pools of P and path analysis used to partition the direct and indirect contribution of soil P fractions to available P.The results show that available P increased significantly with long-term succession,while both sodium bicarbonate-extractable P(NaHCO_(3)-P)and sodium hydroxide-extractable P(NaOHP)fractions were reduced in early successional stages and increased in late stages.Compared to the primary forest,concentrations of P fractions in the four stages significantly decreased except for HCl-P,indicating that soil P supplements over the long-term did not return to primary forest levels.The results of related analysis also showed that NaHCO_(3)-P_(i)levels were significantly related to available phosphorus.According to the path analysis coefficient,NaHCO_(3)-P_(i)exhibited the highest effect on available P among eight P fractions;the indirect effects of other P fractions via NaHCO_(3)-P_(i)were larger than those with other P fractions.Overall,this study suggests that soil P bioavailability gradually improved during natural vegetation restoration on clear-cut sites mainly through the increase of NaHCO_(3)-P,where phosphorous is immediately available,and subsequently available phosphorus Na OH-P.

Changes in Phosphorus Fractions and Nitrogen Forms During Composting of Pig Manure with Rice Straw

The study was conducted to reveal P fractions and N forms changing characters during composting of pig manure with rice straw.During composting,the NH 4 ＋-N concentration decreased and reached at a relatively low value（〈400 mg kg-1） in the final compost,while the NO 3--N concentration increased.Total N losses mainly occurred during thermophilic phase due to the high temperature,the high NH 4 ＋-N concentration and the increase of pH value.Labile inorganic P was dominated in the pig manure and initial compost mixture.During composting,the proportion of labile inorganic P of total extracted P decreased,while the proportion of Fe＋Al-bound P,Ca＋Mg-bound P and residual P increased.The evolutions of the proportion of labile inorganic P,Fe＋Al-bound P and Ca＋Mg-bound P were well correlated with the changes of pH value,organic matter and C/N ratio.Therefore,composting could increase the concentration of N and P and decrease the presence of NH 4 ＋-N and labile P fractions which might cause environmental issues following land application.

Phosphorus fractions of fertiliser-derived P in an allophanic soil under Pinus radiata seedlings grown with broom and ryegrass

Changes in phosphorus （P） fractions in a P deficient allophanic soil under P. radiata seedlings grown with broom （Cytisus scoparius L.） and ryegrass （Lolium multiflorum） in pots were studied 14 months after the application of triple superphosphate at the rates of 0, 50, and 100 pg.g^-1, to determine the fate of fertiliser-derived P in the rhizosphere soils. Application of P fertiliser increased NaOH-Pi, NaOH-Po, and H2SO4-Pi concentrations in the soil, but decreased the residual-P concentration. The resin-Pi concentration, which is ex- tremely low in this soil （1 to μgg^-1 ）, remained the same. The majority of the added fertiliser P was however recovered in the NaOH-Pi fraction （40%-49%）. This is due to the high P fixation in this soil （92%）. The second highest P recovery was in NaOH-Po fraction （7%-19%）. Under P deficient condition or addition at the rate of 0 μg.g^-6, the NaOH-Pi concentration in the radiata rhizosphere soil was lower than that in the bulk soil and broom and grass rhizosphere soils. This may be due to higher oxalate production by the roots and mycorrhiza under P deficient conditions which released some ＆the P fixed to the soils in the rhizosphere, which needs to be tested in future studies.

Impacts of soil properties on phosphorus adsorption and fractions in purple soils

Information on phosphorus(P) adsorption and its impacts on the redistribution of the P fraction in soil profiles are important for environmental management under intensive agricultural practices.To clarify the dominant factors influencing soil phosphorus adsorption in an Entisol(locally known as purple soil), P adsorption experiments were conducted in Sichuan Basin of southwestern China for cropland and woodland soils with acidic, neutral and calcareous origins throughout their profile. After various doses of P were added during incubation experiments, soil P fractions were also analyzed. The results showed that there were no significant differences in Fe-oxides and P adsorption along the vertical gradients. Agricultural practices and lower p H conditions reduced the P adsorption capacity of purple soils throughout the soil profiles. For acidic and neutral purple soil profiles, the P adsorption capability was mainly influenced by Fe-oxides and soil texture. Ca-bound P and Fe-Al-bound P represented the majority of the total inorganic P of calcareous soils.There was a saturation of adsorption capacity by sesquioxide and a high risk of dissoluble reactive P(NH_4 Cl-P) being released out of the soil profile in acidic and neutral purple soils after the greatest P addition, indicated by the higher proportions of NH_4 Cl-P(over 40%) and decreasing Fe-Al-P fraction.P fractions migrated with greater difficulty in calcareous purple soil profiles as Ca-P fraction peaked over 65% when adding a P dose at or greater than 80 g P kg^(-1), indicating the high potential of P adsorption.The X-Ray Diffraction analysis also verified the formation of brushite. Adaptive management practices should be designed to alleviate P losses for acidic and neutral purple soils.

Phosphorus fractions of fertiliser-derived P in an allophanic soil under Pinus radiata seedlings grown with broom and ryegrass

Changes in phosphorus (P) fractions in a P deficient allophanic soil under P. radiata seedlings grown with broom (Cytisus scoparius L.) and ryegrass (Lolium multiflorum) in pots were studied 14 months after the application of triple superphosphate at the rates of 0, 50, and 100 μg·g-1, to determine the fate of fertiliser-derived P in the rhizosphere soils. Application of P fertiliser increased NaOH-Pi, NaOH-Po, and H2SO4-Pi concentrations in the soil, but decreased the residual-P concentration. The resin-Pi concentration, which is extremely low in this soil (1 to 3 μg·g-1 ), remained the same. The majority of the added fertiliser P was however recovered in the NaOH-Pi fraction (40%?49%). This is due to the high P fixation in this soil (92%). The second highest P recovery was in NaOH-Po fraction (7%?19%). Under P deficient condition or addition at the rate of 0 μg·g-1, the NaOH-Pi concentration in the radiata rhizosphere soil was lower than that in the bulk soil and broom and grass rhizosphere soils. This may be due to higher oxalate production by the roots and mycorrhiza under P deficient conditions which released some of the P fixed to the soils in the rhizosphere, which needs to be tested in future studies.