Bacterial resistance is today a matter of great medical concern,so it is urgent to investigate alternatives to alleviate it.Photodynamic inactivation(PDI)is a method that has been endorsed to inactivate diferent patho...Bacterial resistance is today a matter of great medical concern,so it is urgent to investigate alternatives to alleviate it.Photodynamic inactivation(PDI)is a method that has been endorsed to inactivate diferent pathogens,including bacteria,fungi and viruses.PDI is achieved by using a photosensitizer(PS)molecule which generates reactive oxygen species under visible or UV ra-diation.We use visible light and UV-A radiation to excite four commercial PSs(methylene blue,rose bengal,riboflavin and curcumin),and nanopartides synthesized in our laboratory.Despite these PSs having been thoroughly studied in the past by other research groups,in order to compare their effects in an appropriate way,we matched the number of photons they absorb.We found that methylene blue leads to the major inactivation of Escherichia coli.Furthermore,we evaluated the production of singlet axygen and hydroxyl radicals in the photoinactivation process.展开更多
基金supported by CONACyT,Mexico under Grant A1-S-8125 and Fideicomiso SEPCINVESTAV(FIDSC2018/72).
文摘Bacterial resistance is today a matter of great medical concern,so it is urgent to investigate alternatives to alleviate it.Photodynamic inactivation(PDI)is a method that has been endorsed to inactivate diferent pathogens,including bacteria,fungi and viruses.PDI is achieved by using a photosensitizer(PS)molecule which generates reactive oxygen species under visible or UV ra-diation.We use visible light and UV-A radiation to excite four commercial PSs(methylene blue,rose bengal,riboflavin and curcumin),and nanopartides synthesized in our laboratory.Despite these PSs having been thoroughly studied in the past by other research groups,in order to compare their effects in an appropriate way,we matched the number of photons they absorb.We found that methylene blue leads to the major inactivation of Escherichia coli.Furthermore,we evaluated the production of singlet axygen and hydroxyl radicals in the photoinactivation process.
