Maize growth and development are regulated by light quality,intensity and photoperiod.Cryptochromes are blue/ultraviolet-A light receptors involved in stem elongation,shade avoidance,and photoperiodic flowering.To inv...Maize growth and development are regulated by light quality,intensity and photoperiod.Cryptochromes are blue/ultraviolet-A light receptors involved in stem elongation,shade avoidance,and photoperiodic flowering.To investigate the function of cryptochrome 1(CRY1) in maize,where it is encoded by Zm CRY1,we obtained two Zm CRY1a genes(Zm CRY1a1 and Zm CRY1a2),both of which share the highest similarity with other gramineous plants,in particular rice CRY1a by phylogenetic analysis.In Arabidopsis,overexpression of Zm CRY1a genes promoted seedling de-etiolation under blue and white light,resulting in dwarfing of mature plants.In seedlings of the maize inbred line Zong 31(Zm CRY1aOE),overexpression of Zm CRY1a genes caused a reduction in the mesocotyl and first leaf sheath lengths due to down-regulation of genes influencing cell elongation.In mature transgenic maize plants,plant height,ear height,and internode length decreased in response to overexpression of Zm CRY1a genes.Expression of Zm CRY1a were insensitive to low blue light(LBL)-induced shade avoidance syndrome(SAS) in Arabidopsis and maize.This prompted us to investigate the regulatory role of the gibberellin and auxin metabolic pathways in the response of Zm CRY1a genes to LBL treatment.We confirmed a link between Zm CRY1a expression and hormonal influence on the growth and development of maize under LBL-induced SAS.These results reveal that Zm CRY1a has a relatively conservative function in regulating maize photomorphogenesis and may guide new strategies for breeding high density-tolerant maize cultivars.展开更多
Light is a fundamental environmental factor for living organisms on earth—not only as a primary energy source but also as an informational signal.In fungi,light can be used as an indicator for both time and space to ...Light is a fundamental environmental factor for living organisms on earth—not only as a primary energy source but also as an informational signal.In fungi,light can be used as an indicator for both time and space to control important physiological and morphological responses.Botrytis cinerea(B.cinerea)is a devastating phytopathogenic fungus that exploits light cues to optimize virulence and the balance between conidiation and sclerotia development,thereby improving its dispersal and survival in ecosystems.However,the components and mechanisms underlying these processes remain obscure.Here,we identify a novel light-signaling component in B.cinerea,BcCfaS,which encodes a putative cyclopropane fatty-acyl-phospholipid synthase.BcCfaS is strongly induced by light at the transcriptional level and plays a crucial role in regulating photomorphogenesis.Deletion of BcCfaS results in reduced vegetative growth,altered colony morphology,impaired sclerotial development,and enhanced conidiation in a lightdependent manner.Moreover,the mutant exhibits serious defects in stress response and virulence on the host.Based on a lipidomics analysis,a number of previously unknown fungal lipids and many BcCfaS-regulated lipids are identified in B.cinerea,including several novel phospholipids and fatty acids.Importantly,we find that BcCfaS controls conidiation and sclerotial development by positively regulating methyl jasmonate(MeJA)synthesis to activate the transcription of light-signaling components,revealing for the first time the metabolic base of photomorphogenesis in fungi.Thus,we propose that BcCfaS serves as an integration node for light and lipid metabolism,thereby providing a regulatory mechanism by which fungi adapt their development to a changing light environment.These new findings provide an important target for antifungal design to prevent and control fungal disease.展开更多
Soybean(Glycine max)responds to ambient light variation by undergoing multiform morphological alterations,influencing its yield potential and stability in the field.Phytochromes(PHYs)are plant-specific red(R)and far-r...Soybean(Glycine max)responds to ambient light variation by undergoing multiform morphological alterations,influencing its yield potential and stability in the field.Phytochromes(PHYs)are plant-specific red(R)and far-red(FR)light photoreceptors mediating photomorphogenesis and photoperiodic flowering.As an ancient tetraploid,soybean harbors four PHYA,two PHYB,and two PHYE paralogs.Except for GmPHYA2/E4 and GmPHYA3/E3,which have been identified as photoperiod-dependent flowering repressors,the functions of GmPHYs are still largely unclear.We generated a series of individual or combined mutations targeting the GmPHYA or GmPHYB genes using CRISPR/Cas9 technology.Phenotypic analysis revealed that GmPHYB1 mediates predominantly R-light induced photomorphogenesis,whereas GmPHYA2/E4 and GmPHYA3/E3,followed by GmPHYA1 and GmPHYB2,function redundantly and additively in mediating FR light responses in seedling stage.GmPHYA2/E4 and GmPHYA3/E3,with weak influence from GmPHYA1 and GmPHYA4,delay flowering time under natural long-day conditions.This study has demonstrated the diversified functions of GmPHYAs and GmPHYBs in regulating light response,and provides a core set of phytochrome mutant alleles for characterization of their functional mechanisms in regulating agronomic traits of soybean.展开更多
Plants have evolved and perfected a series of light receptors to feel the light at different bands and regulate the expression, modification and interaction of related genes in plants through signal transduction. So f...Plants have evolved and perfected a series of light receptors to feel the light at different bands and regulate the expression, modification and interaction of related genes in plants through signal transduction. So far, many photoreceptors have been identified in plants, UVR8 has recently been identified as a receptor for UV-B light. This paper cloned a WD40 gene related to UVR8 protein subunit, named RrRUP2, based on the Rosa rugose transcriptome data, using Rosa rugose “Zi zhi” as experimental materials. The full length of cDNA of the gene was obtained by RT-PCR and RACE methods. The total length of this gene is 1173 bp, and it encodes 390 amino acids. After bioinformatics analysis, the molecular formula C3415H5659N1173O1434S313 was predicted;the relative molecular weight was 96129.27 Da;the theoretical isoelectric point PI value was 5.00;and its instability index was 47.06. The total average hydrophobic index was 0.750. In the secondary structure of RrRUP2 protein, there are 10 α-helix, 45 β-helix, 181 Random coil, and 154 Extended strand. Gene Bank Blast results showed that the amino acid sequence encoded by RrRUP2 was more than 90% homologous with the RUP2 protein of Rosa chinensis, Fragaria, Malus, Pyrus, Prunus, Juglans, Arabidopsis and Tobacco, so it can be inferred that the RrRUP2 gene is a WD repeat-containing protein. Regarding to fluorescence quantitative expression analysis of RrRUP2, we find its experssion pattern is corresponded with the accumulation of anthocyanins.展开更多
Coordinated morphogenic adaptation of growing plants is critical for their survival and propagation under fluctuating environments.Plant morphogenic responses to light and warm temperatures,termed photomorphogenesis a...Coordinated morphogenic adaptation of growing plants is critical for their survival and propagation under fluctuating environments.Plant morphogenic responses to light and warm temperatures,termed photomorphogenesis and thermomorphogenesis,respectively,have been extensively studied in recent decades.During photomorphogenesis,plants actively reshape their growth and developmental patterns to cope with changes in light regimes.Accordingly,photomorphogenesis is closely associated with diverse growth hormonal cues.Notably,accumulating evidence indicates that light-directed morphogenesis is profoundly affected by two recently identified phytochemicals,karrikins(KARs)and strigolactones(SLs).KARs and SLs are structurally related butenolides acting as signaling molecules during a variety of developmental steps,including seed germination.Their receptors and signaling mediators have been identified,and associated working mechanisms have been explored using gene-deficient mutants in various plant species.Of particular interest is that the KAR and SL signaling pathways play important roles in environmental responses,among which their linkages with photomorphogenesis are most comprehensively studied during seedling establishment.In this review,we focus on how the phytochemical and light signals converge on the optimization of morphogenic fitness.We also discuss molecular mechanisms underlying the signaling crosstalks with an aim of developing potential ways to improve crop productivity under climate changes.展开更多
Phytochrome has fascinated plant scientists since its discovery in 1959-1960 by the Beltsville research group of the United States Department of Agriculture. Studies in the first 20 years had evidenced that phytochrom...Phytochrome has fascinated plant scientists since its discovery in 1959-1960 by the Beltsville research group of the United States Department of Agriculture. Studies in the first 20 years had evidenced that phytochrome acts as an universal regulator in plant life adapting its behavior to the environmental light, and developed widely the physiological understanding of phytochrome action. In the following 20 years, some thirty world_wide major laboratories have published over two hundred papers a year on various aspects of the subject, and they are making steady progress. The authors’ work has also contributed to the following aspects: coaction of phytochrome and phytohormone in photomorphogenesis, phytochrome purification, phytochrome regulation of male fertility, as well as phytochrome A gene analysis and expression in photoperiod sensitive genic male sterile rice. In the recent decade significant advances have been made in studies on phytochrome molecules, genes and signal transduction in phytochrome response. This is largely due to the advances in molecular genetics, where experiments using mutants and transgenic plants, particularly in Arabidopsis, that have led to the significant insights at the molecular level. The topics in this review include:(1) Discovery of phytochrome; (2) Functions of phytochrome; (3) Phytochrome molecules; (4) Phytochrome regulation in gene expression.展开更多
基金supported by the National Natural Science Foundation of China (31871709)the Construction of Support System for National Agricultural Green Development Advance Region of Qushui County,Tibet,China (QYXTZX-LS2022-01)+1 种基金the Key Project of Beijing Natural Science Foundation (6151002)the Startup Grants of Henan Agricultural University (30501038,30500823)。
文摘Maize growth and development are regulated by light quality,intensity and photoperiod.Cryptochromes are blue/ultraviolet-A light receptors involved in stem elongation,shade avoidance,and photoperiodic flowering.To investigate the function of cryptochrome 1(CRY1) in maize,where it is encoded by Zm CRY1,we obtained two Zm CRY1a genes(Zm CRY1a1 and Zm CRY1a2),both of which share the highest similarity with other gramineous plants,in particular rice CRY1a by phylogenetic analysis.In Arabidopsis,overexpression of Zm CRY1a genes promoted seedling de-etiolation under blue and white light,resulting in dwarfing of mature plants.In seedlings of the maize inbred line Zong 31(Zm CRY1aOE),overexpression of Zm CRY1a genes caused a reduction in the mesocotyl and first leaf sheath lengths due to down-regulation of genes influencing cell elongation.In mature transgenic maize plants,plant height,ear height,and internode length decreased in response to overexpression of Zm CRY1a genes.Expression of Zm CRY1a were insensitive to low blue light(LBL)-induced shade avoidance syndrome(SAS) in Arabidopsis and maize.This prompted us to investigate the regulatory role of the gibberellin and auxin metabolic pathways in the response of Zm CRY1a genes to LBL treatment.We confirmed a link between Zm CRY1a expression and hormonal influence on the growth and development of maize under LBL-induced SAS.These results reveal that Zm CRY1a has a relatively conservative function in regulating maize photomorphogenesis and may guide new strategies for breeding high density-tolerant maize cultivars.
基金supported by the National Natural Science Foundation of China(31930086 and 32172642)the National Key Research and Development(R&D)Program of China(2016YFD0400902 and 2021YFD2100505).
文摘Light is a fundamental environmental factor for living organisms on earth—not only as a primary energy source but also as an informational signal.In fungi,light can be used as an indicator for both time and space to control important physiological and morphological responses.Botrytis cinerea(B.cinerea)is a devastating phytopathogenic fungus that exploits light cues to optimize virulence and the balance between conidiation and sclerotia development,thereby improving its dispersal and survival in ecosystems.However,the components and mechanisms underlying these processes remain obscure.Here,we identify a novel light-signaling component in B.cinerea,BcCfaS,which encodes a putative cyclopropane fatty-acyl-phospholipid synthase.BcCfaS is strongly induced by light at the transcriptional level and plays a crucial role in regulating photomorphogenesis.Deletion of BcCfaS results in reduced vegetative growth,altered colony morphology,impaired sclerotial development,and enhanced conidiation in a lightdependent manner.Moreover,the mutant exhibits serious defects in stress response and virulence on the host.Based on a lipidomics analysis,a number of previously unknown fungal lipids and many BcCfaS-regulated lipids are identified in B.cinerea,including several novel phospholipids and fatty acids.Importantly,we find that BcCfaS controls conidiation and sclerotial development by positively regulating methyl jasmonate(MeJA)synthesis to activate the transcription of light-signaling components,revealing for the first time the metabolic base of photomorphogenesis in fungi.Thus,we propose that BcCfaS serves as an integration node for light and lipid metabolism,thereby providing a regulatory mechanism by which fungi adapt their development to a changing light environment.These new findings provide an important target for antifungal design to prevent and control fungal disease.
基金supported by the National Natural Science Foundation of China(31871705,32072091)the Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Sciencesthe Central Public-interest Scientific Institution Basal Research Fund。
文摘Soybean(Glycine max)responds to ambient light variation by undergoing multiform morphological alterations,influencing its yield potential and stability in the field.Phytochromes(PHYs)are plant-specific red(R)and far-red(FR)light photoreceptors mediating photomorphogenesis and photoperiodic flowering.As an ancient tetraploid,soybean harbors four PHYA,two PHYB,and two PHYE paralogs.Except for GmPHYA2/E4 and GmPHYA3/E3,which have been identified as photoperiod-dependent flowering repressors,the functions of GmPHYs are still largely unclear.We generated a series of individual or combined mutations targeting the GmPHYA or GmPHYB genes using CRISPR/Cas9 technology.Phenotypic analysis revealed that GmPHYB1 mediates predominantly R-light induced photomorphogenesis,whereas GmPHYA2/E4 and GmPHYA3/E3,followed by GmPHYA1 and GmPHYB2,function redundantly and additively in mediating FR light responses in seedling stage.GmPHYA2/E4 and GmPHYA3/E3,with weak influence from GmPHYA1 and GmPHYA4,delay flowering time under natural long-day conditions.This study has demonstrated the diversified functions of GmPHYAs and GmPHYBs in regulating light response,and provides a core set of phytochrome mutant alleles for characterization of their functional mechanisms in regulating agronomic traits of soybean.
文摘Plants have evolved and perfected a series of light receptors to feel the light at different bands and regulate the expression, modification and interaction of related genes in plants through signal transduction. So far, many photoreceptors have been identified in plants, UVR8 has recently been identified as a receptor for UV-B light. This paper cloned a WD40 gene related to UVR8 protein subunit, named RrRUP2, based on the Rosa rugose transcriptome data, using Rosa rugose “Zi zhi” as experimental materials. The full length of cDNA of the gene was obtained by RT-PCR and RACE methods. The total length of this gene is 1173 bp, and it encodes 390 amino acids. After bioinformatics analysis, the molecular formula C3415H5659N1173O1434S313 was predicted;the relative molecular weight was 96129.27 Da;the theoretical isoelectric point PI value was 5.00;and its instability index was 47.06. The total average hydrophobic index was 0.750. In the secondary structure of RrRUP2 protein, there are 10 α-helix, 45 β-helix, 181 Random coil, and 154 Extended strand. Gene Bank Blast results showed that the amino acid sequence encoded by RrRUP2 was more than 90% homologous with the RUP2 protein of Rosa chinensis, Fragaria, Malus, Pyrus, Prunus, Juglans, Arabidopsis and Tobacco, so it can be inferred that the RrRUP2 gene is a WD repeat-containing protein. Regarding to fluorescence quantitative expression analysis of RrRUP2, we find its experssion pattern is corresponded with the accumulation of anthocyanins.
基金supported by the Leaping Research Program(NRF-2021R1A2B5B03001476 to C.M.P.)provided by the National Research Foundation(NRF)of Koreaa grant from Kyung Hee University in 2023(KHU-20230886 to Y.J.P.).
文摘Coordinated morphogenic adaptation of growing plants is critical for their survival and propagation under fluctuating environments.Plant morphogenic responses to light and warm temperatures,termed photomorphogenesis and thermomorphogenesis,respectively,have been extensively studied in recent decades.During photomorphogenesis,plants actively reshape their growth and developmental patterns to cope with changes in light regimes.Accordingly,photomorphogenesis is closely associated with diverse growth hormonal cues.Notably,accumulating evidence indicates that light-directed morphogenesis is profoundly affected by two recently identified phytochemicals,karrikins(KARs)and strigolactones(SLs).KARs and SLs are structurally related butenolides acting as signaling molecules during a variety of developmental steps,including seed germination.Their receptors and signaling mediators have been identified,and associated working mechanisms have been explored using gene-deficient mutants in various plant species.Of particular interest is that the KAR and SL signaling pathways play important roles in environmental responses,among which their linkages with photomorphogenesis are most comprehensively studied during seedling establishment.In this review,we focus on how the phytochemical and light signals converge on the optimization of morphogenic fitness.We also discuss molecular mechanisms underlying the signaling crosstalks with an aim of developing potential ways to improve crop productivity under climate changes.
文摘Phytochrome has fascinated plant scientists since its discovery in 1959-1960 by the Beltsville research group of the United States Department of Agriculture. Studies in the first 20 years had evidenced that phytochrome acts as an universal regulator in plant life adapting its behavior to the environmental light, and developed widely the physiological understanding of phytochrome action. In the following 20 years, some thirty world_wide major laboratories have published over two hundred papers a year on various aspects of the subject, and they are making steady progress. The authors’ work has also contributed to the following aspects: coaction of phytochrome and phytohormone in photomorphogenesis, phytochrome purification, phytochrome regulation of male fertility, as well as phytochrome A gene analysis and expression in photoperiod sensitive genic male sterile rice. In the recent decade significant advances have been made in studies on phytochrome molecules, genes and signal transduction in phytochrome response. This is largely due to the advances in molecular genetics, where experiments using mutants and transgenic plants, particularly in Arabidopsis, that have led to the significant insights at the molecular level. The topics in this review include:(1) Discovery of phytochrome; (2) Functions of phytochrome; (3) Phytochrome molecules; (4) Phytochrome regulation in gene expression.
