Physiological mechanisms underlying reduced photosynthesis in wheat leaves grown in the field under conditions of nitrogen and water deficiency

Reduced photosynthesis results directly from nitrogen or water deficiency in wheat plants,and leads to a decrease in grain yield.In this study,by measuring the effects of water and N deficiencies,both individually and combined,we characterized the responses of wheat(Triticum aestivum L.Yumai 49-198)plants to these two deficiencies using physiological measurements and comparative proteomics.Significant decreases in grain yield and leaf photosynthetic performance were observed in all deficiency conditions,and 106 photosynthetic proteins that showed responses were identified.Nitrogen deficiency induced the least change in photosynthetic proteins,and similar changes in most of these proteins were also observed for the combined nitrogen and water deficiencies.Water deficiency induced the largest change in photosynthetic proteins and resulted in the lowest 1000-kernel weight.Severe decreases in photosynthesis in both the water-deficiency and combined N and water deficiency groups were reflected mainly in an imbalanced ATP/NADPH ratio associated with the light reaction,which influences carbon metabolism in the Calvin cycle.Photorespiration was respectively stimulated or inhibited by N or water deficiency,while suppression of photorespiratory flux and activation of nitrogen recycling were observed in the combined N and water deficiency treatments.Comparison of photosynthetic proteins between experimental sites suggested that precipitation affected linear electron flow in the photoreaction,and thus photosynthetic efficiency.Our results provide a baseline for future studies of the roles of these photosynthetic proteins in the response to N or water deficiency and their effect on 1000-kernel weight.

Arbuscular mycorrhizal fungi improve biomass, photosynthesis, and water use efficiency of Opuntia ficus-indica (L.) Miller under different water levels

Opuntia ficus-indica(L.)Miller is a CAM(crassulacean acid metabolism)plant with an extraordinary capacity to adapt to drought stress by its ability to fix atmospheric CO_(2) at nighttime,store a significant amount of water in cladodes,and reduce root growth.Plants that grow in moisture-stress conditions with thick and less fine root hairs have a strong symbiosis with arbuscular mycorrhizal fungi(AMF)to adapt to drought stress.Water stress can limit plant growth and biomass production,which can be rehabilitated by AMF association through improved physiological performance.The objective of this study was to investigate the effects of AMF inoculations and variable soil water levels on the biomass,photosynthesis,and water use efficiency of the spiny and spineless O.ficus-indica.The experiment was conducted in a greenhouse with a full factorial experiment using O.ficus-indica type(spiny or spineless),AMF(presence or absence),and four soil water available(SWA)treatments through seven replications.Water treatments applied were 0%–25%SWA(T1),25%–50%SWA(T2),50%–75%SWA(T3),and 75%–100%SWA(T4).Drought stress reduced biomass and cladode growth,while AMF colonization significantly increased the biomass production with significant changes in the physiological performance of O.ficus-indica.AMF presence significantly increased biomass of both O.ficus-indica plant types through improved growth,photosynthetic water use efficiency,and photosynthesis.The presence of spines on the surface of cladodes significantly reduced the rate of photosynthesis and photosynthetic water use efficiency.Net photosynthesis,photosynthetic water use efficiency,transpiration,and stomatal conductance rate significantly decreased with increased drought stress.Under drought stress,some planted mother cladodes with the absence of AMF have not established daughter cladodes,whereas AMF-inoculated mother cladodes fully established daughter cladodes.AMF root colonization significantly increased with the decrease of SWA.AMF caused an increase in biomass production,increased tolerance to drought stress,and improved photosynthesis and water use efficiency performance of O.ficus-indica.The potential of O.ficus-indica to adapt to drought stress is controlled by the morpho-physiological performance related to AMF association.

Interspecific Transition Among Caragana microphylla, C. davazamcii and C. korshinskii Along Geographic Gradient. Ⅱ. Characteristics of Photosynthesis and Water Metabolism

The characteristics of photosynthesis and water metabolism of Caragana microphylla Lam.,C. davazamcii Sancz. and C. korshinskii Kom. populations in different sites (117.6o-105.7o E, 44.6o-38.8o N)were studied. (1) From the east to the west, the responses of the three species to photosyntheticallyavailable radiation (PAR) in net photosynthesis rate increased, the relative humidity of the air whichcorresponded to the occurrence of maximum photosynthesis rate decreased, and the corresponding airtemperature increased. Along the same gradient, the before-noon superiority of the photosynthesis be-came evident, and the photosynthesis rate and the light use efficiency (LUE ) increased, while the transp-iration rate decreased, thus the water use efficiency (WUE ) increased notably, and the leaf water contentdecreased gradually. From the east to the west, the plants took a water-saving strategy step by step withhigher photosynthesis rate and lower transpiration rate. These physiological changes in the plants wereadaptable to the conditions of light, temperature and humidity in the habitat of the plants, and might be thebiological foundation for the geographical transition among C. microphylla , C. davazamcii and C. korshinskii.(2) The adaptation of photosynthetic system of C. microphylla , C. davazamcii and C. korshinskii to PAR, airhumidity and temperature exhibited the interspecific continuity, which was consistent with theenvironmental gradient. In different species and different sites, the diurnal changes of net photosynthesisrate, the daily cumulative value of net photosynthesis, the diurnal changes of transpiration rate, the dailycumulative value of transpiration, the water use efficiency and the diurnal changes of leaf water contentvaried with longitudinal descent (from the east to the west). The characteristics of photosynthesis andwater metabolism indicated that the geographical transition among C. microphylla , C. davazamcii and C.korshinskii was in gradual change, and these three species formed a geographical cline.

Species- and Habitat-variability of Photosynthesis, Transpiration and Water Use Efficiency of Different Plant Species in Maowusu Sand Area

Photosynthesis ( P n ), transpiration ( E ) and water use efficiency ( WUE ) of more than 66 arid sand species from different environmental habitats, shifting sand dune, fixed sand dune, lowland and wetland in the Maowusu Sand Area were analyzed and the relation among these characteristics and the resource utilization efficiency, taxonomic categories and growth forms of the species were assessed. The results showed that species from Chenopodiaceae, Gramineae, Leguminosae which possessed the C 4 photosynthesis pathway, or C 3 pathway and also with nitrogen_fixation capacities had higher or the highest P n values, i.e., 20～30 μmol CO 2·m -2 ·s -1 , while that of evergreen shrub of Pinaceae had the lowest P n values, i.e., 0～5 μmol CO 2·m -2 ·s -1 . Those species from Compositae, Scrophulariaceae, and Gramineae with C 3 pathway but no N_fixation capacity had the highest E rates, i.e., 20～30 mmol H 2O·m -2 ·s -1 and again the evergreen shrub together with some species from Salicaceae and Compositae had the lowest E rates, i.e., 0～5 mmol H 2O·m -2 ·s -1 . Species from Leguminosae, Gramineae and Chenopodiaceae with C 4 pathway or C 3 pathway with N_fixation capacity, both shrubs and grasses, generally had higher WUE . However, even the physiological traits of the same species were habitat_ and season_specific. The values of both P n and E in late summer were much higher than those in early summer, with average increases of 26%, 40% respectively in the four habitats. WUE in late summer was, however, 12% lower. Generally, when the environments became drier as a result of habitats changed, i.e., in the order of wetland, lowland, fixed sand dune and shifting sand dune, P n and E decreased but WUE increased.

Irrigation mitigates the heat impacts on photosynthesis during grain filling in maize

Elevating soil water content(SWC)through irrigation was one of the simple mitigation measures to improve crop resilience to heat stress.The response of leaf function,such as photosynthetic capacity based on chlorophyll fluorescence during the mitigation,has received limited attention,especially in field conditions.A two-year field experiment with three treatments(control treatment(CK),high-temperature treatment(H),and high-temperature together with elevating SWC treatment(HW))was carried out during grain filling with two maize hybrids at a typical station in North China Plain.Averagely,the net photosynthetic rate(Pn)was improved by 20%,and the canopy temperature decreased by 1–3℃ in HW compared with in H in both years.Furthermore,the higher SWC in HW significantly improved the actual photosynthetic rate(Phi2),linear electron flow(LEF),variable fluorescence(F_(v)),and the maximal potential quantum efficiency(F_(v)/F_(m))for both hybrids.Meanwhile,different responses in chlorophyll fluorescence between hybrids were also observed.The higher SWC in HW significantly improved thylakoid proton conductivity(g H^(+))and the maximal fluorescence(F_(m))for the hybrid ZD958.For the hybrid XY335,the proton conductivity of chloroplast ATP synthase(v H^(+))and the minimal fluorescence(Fo)was increased by the SWC.The structural equation model(SEM)further showed that SWC had significantly positive relationships with Pn,LEF,and F_(v)/F_(m).The elevating SWC alleviated heat stress with the delayed leaf senescence to prolong the effective period of photosynthesis and enhanced leaf photosynthetic capacity by improving Phi2,LEF,Fv,and F_(v)/F_(m).This research demonstrates that elevating SWC through enhancing leaf photosynthesis during grain filling would be an important mitigation strategy for adapting to the warming climate in maize production.

Beneficial effects of silicon on photosynthesis of tomato seedlings under water stress

Silicon can improve drought tolerance of plants,but the mechanism still remains unclear.Previous studies have mainly concentrated on silicon-accumulating plants,whereas less work has been conducted in silicon-excluding plants,such as tomato（Solanum lycopersicum L.）.In this study,we investigated the effects of exogenous silicon（2.5 mmol L^（-1））on the chlorophyll fluorescence and expression of photosynthesis-related genes in tomato seedlings（Zhongza 9）under water stress induced by 10%（w/v）polyethylene glycol（PEG-6000）.The results showed that under water stress,the growth of shoot and root was inhibited,and the chlorophyll and carotenoid concentrations were decreased,while silicon addition improved the plant growth and increased the concentrations of chlorophyll and carotenoid.Under water sterss,chlorophyll fluorescence parameters such as PSII maximum photochemical efficiency（F_v/F_m）,effective quantum efficiency,actual photochemical quantum efficiency（Ф_（PSII））,photosynthetic electron transport rate（ETR）,and photochemical quenching coefficient（q_P）were decreased;while these changes were reversed in the presence of added silicon.The expressions of some photosynthesis-related genes including PetE,PetF,PsbP,PsbQ,PsbW,and Psb28 were down-regulated under water stress,and exogenous Si could partially up-regulate their expressions.These results suggest that silicon plays a role in the alleviation of water stress by modulating some photosynthesis-related genes and regulating the photochemical process,and thus promoting photosynthesis.

Effects of post-silking water deficit on the leaf photosynthesis and senescence of waxy maize

Waxy maize is widely cultivated under rainfed conditions and frequently suffers water shortage during the late growth stage.In this study,a pot trial was conducted to examine the effects of post-silking drought on leaf photosynthesis and senescence and its influence on grain yield.Two waxy maize hybrids,Suyunuo 5(SYN5)and Yunuo 7(YN7),were grown under the control and drought(soil moisture content was 70–80%and 50–60%,respectively)conditions after silking in 2016 and 2017.The decrease in yield was 11.1 and 15.4%for YN7 and SYN5,respectively,owing to the decreased grain weight and number.Post-silking dry matter accumulation was reduced by 27.2%in YN7 and 26.3%in SYN5.The contribution rate of pre-silking photoassimilates transferred to grain yield was increased by 15.6%in YN7 and 10.2%in SYN5,respectively.Post-silking drought increased the malondialdehyde content,but decreased the contents of water,soluble protein,chlorophyll,and carotenoid in the leaves.The weakened activities of enzymes involved in photosynthesis(ribulose-1,5-bisphosphate carboxylase and phosphoenolpyruvate carboxylase)and antioxidant system(catalase,superoxide dismutase and peroxidase)reduced the photosynthetic rate(Pn)and accelerated leaf senescence.The correlation results indicated that reduced Pn and catalase activity and increased malondialdehyde content under drought conditions induced the decrease of post-silking photoassimilates deposition,ultimately resulted in the grain yield loss.

The Pharmacologic Intensification of the Water Dissociation Process, or Human Photosynthesis, and Its Effect over the Recovery Mechanisms in Tissues Affected by Bloodshed of Diverse Etiology

The photoreceptor layer of the human retina has several characteristics that are unique. Their energy requirements are the highest in the organism;in proportion, rods and cones require 10-fold the energy consumed by the cerebral cortex, 6-fold more than the cardiac muscle, and 3-fold more than the renal cortex. Astonishingly, the photoreceptor layer has no blood vessels at all. So, where is the energy to this tissue coming from? In this article we’ll describe the hitherto unknown explanation.

Effect of Water Deficit Imposed during the Early Developmental Phase on Photosynthesis of Cocoa (Theobroma cacao L.)

A greenhouse study was carried out at Cocoa Research Institute of Nigeria, Ibadan to study the effect of water stress on the four popular cocoa genotypes at the institute. F3 Amazon, T1, T7 and Amelonado were raised under different water regimes (daily, 3-day interval, 5-day interval and 7-day interval) at 100%, 50% and 25% field capacities. Data were collected on the height, leaf area, root length, stomata conductance, photosynthetic rate and water use efficiency of the plants. Results showed that plant performances showed genotypic variation in their response to water stress. Generally, there were linear and positive relationships between water level and values in both physiological and morphological responses of cocoa genotypes.

A study of the soil water potential threshold values to trigger irrigation of ‘Shimizu Hakuto’ peach at pivotal fruit developmental stages

Water management is an important practice that affects fruit size and quality.Effective implementation of irrigation scheduling requires knowledge of the appropriate indicators and thresholds,which are established manly based on the effects of water deficits on final fruit quality.Few studies have focused on the real-time effects of water status on fruit and shoot growth.To establish soil water potential (ψ_(soil)) thresholds to trigger irrigation of peach at pivotal fruit developmental stages,photogrammetry,^(13)C labelling,and other techniques were used in this study to investigate real-time changes in stem diameter,fruit projected area,net leaf photosynthetic rate (P_(n)),and allocation of photoassimilates to fruit under soil water potential conditions ranging from saturation to stress in 6-year-old Shimizu hakuto’peach.Stem growth,fruit growth,and P_n exhibited gradually decreasing sensitivity to water deficits during fruit developmental stages I,II,and III.Stem diameter growth was significantly inhibited whenψ_(soil)dropped to-8.5,-7.6,and-5.4 k Pa,respectively.Fruit growth rate was low,reaching zero when theψ_(soil)was-9.0 to-23.1,-14.9 to-21.4,and-16.5 to-23.3 k Pa,respectively,and P_ndecreased significantly when theψ_(soil)reached-24.2,-22.7,and-20.4 kPa,respectively.In addition,more photoassimilates were allocated to fruit under moderateψ_(soil)conditions (-10.1 to-17.0 k Pa) than under otherψ_(soil)values.Our results revealed threeψ_(soil)thresholds,-10.0,-15.0,and-15.0 kPa,suitable for triggering irrigation during stages I,II,and III,respectively.These thresholds can be helpful for controlling excessive tree vigor,maintaining rapid fruit growth and leaf photosynthesis,and promoting the allocation of more photoassimilates to fruit.

Influence of O-O formation pathways and charge transfer mediator on lipid bilayer membrane-like photoanodes for water oxidation

Inspired by the function of crucial components in photosystemⅡ(PSⅡ),electrochemical and dyesensitized photoelectrochemical(DSPEC)water oxidation devices were constructed by the selfassembly of well-designed amphipathic Ru(bda)-based catalysts(bda=2,2'-bipyrdine-6,6'-dicarbonoxyl acid)and aliphatic chain decorated electrode surfaces,forming lipid bilayer membrane(LBM)-like structures.The Ru(bda)catalysts on electrode-supported LBM films demonstrated remarkable water oxidation performance with different O-O formation mechanisms.However,compared to the slow charge transfer process,the O-O formation pathways did not determine the PEC water oxidation efficiency of the dyesensitized photoanodes,and the different reaction rates for similar catalysts with different catalytic paths did not determine the PEC performance of the DSPECs.Instead,charge transfer plays a decisive role in the PEC water oxidation rate.When an indolo[3,2-b]carbazole derivative was introduced between the Ru(bda)catalysts and aliphatic chain-modified photosensitizer in LBM films,serving as a charge transfer mediator for the tyrosine-histidine pair in PSⅡ,the PEC water oxidation performance of the corresponding photoanodes was dramatically enhanced.

Effects of Different Water and Fertilizer Combinations on Tree Structure,Leaf Parameters and Photosynthesis of Apple Saplings

[ Objective] This study aimed to investigate the effects of different water and fertilizer combinations on apple saplings. [ Method] ＇ Tianhong 2＇ Fuji /SH40/Malus robusta Rehd. was used as the experimental material to analyze the effects of different irrigation modes and combinations of basal fertilizer and dressing fertilizer on tree structure, leaf parameters and photosynthesis of apple saplings. [ Result] The results showed that different water and fertilizer combinations ex- hibited varying effects on tree structure, leaf parameters and photosynthesis of apple saplings. To be specific, applying 432 000 kg/hm2 basal fertilizer, 480 kg/hm2 urea and 915 kg/hmz organic fertilizer ＋ 0 ＋ 915 kg/hm2 organic fertilizer as dressing fertilizer, and 1% urea as leaf fertilizer was conducive to promoting growth of branches and leaves, increasing leaf thickness, individual leaf area and SPAD, and improving photosynthesis of apple saplings under half root irrigation and whole root irrigation conditions. In addition, the effects were more significant under whole root irrigation conditions. [ Conclusion] Selecting the appropriate water and fertilizer combination is conducive to the growth of apple saplings.

Response of Photosynthesis, Growth, Carbon Isotope Discrimination and Osmotic Tolerance of Rice to Elevated CO_2

Four rice ( Oryza sativa L.) cultivars 'IR72', 'Tesanai 2', 'Guichao 2' and 'IIyou 4480' were grown in two plastic house (15 m×3 m) with 35 μmol/mol and 60 μmol/mol CO 2 concentration which was controlled by computer. As compared with rice at ambient 35 μmol/mol CO 2, the changes in photosynthetic rate at elevated CO 2 showed up_regulation ('IR72' and 'Tesanai 2'), stable (unchanged) in 'Guichao 2' and down_regulation type ('IIyou 4480'). Growth rate, panicle weight, integrated water use efficiency (WUE) calculated from Δ 13 C and the capacity of scavenging DPPH · (1,1_diphenyl_2_picrylhydrazyl) free radical were increased at elevated CO 2. An increment in total biomass was observed in three cultivars by elevated CO 2, with the exception of 'IIyou 4480'. Ratios of panicle weight/total biomass were altered to different extents in tested cultivars by elevated CO 2. When leaf segments were subjected to PEG osmotic stress, the electrolyte leakage rate from leaves grown at elevated CO 2 was less than that at 35 μmol/mol CO 2. Those intraspecific variations of rice imply a possibility for selecting cultivars with maximal productivity and high tolerance to stresses adapted to elevated CO 2 in the future.

Leaf Turgor Potential, Plant Growth and Photosynthesis in Organically Fertilized Sweet Corn

A greenhouse pot experiment was conducted using a complete random design with six replications. A pressure-volume curve analysis was employed to study the effects of organic fertilizers on plant water relations in sweet corn (Zea mays L. cv. Honey-bantam) in terms of leaf osmotic concentration (Cs), osmotic potentials at full turgid status (πFT) and at incipient plasmolysis (πIP), and of symplastic (ζsym) and apoplastic (ζapo) fractions in the tissue water compartment in relation to photosynthetic capacity (Pc) and dry mass accumulation. At the seedling stage (day 15), plants with chemical fertilizer treatments showed lower πFT, πIP and ζapo and higher Cs, ζsym and PC than those with organic fertilizer treatments. Compared to PC and relative growth, where values from day 15 to day 75 were first lower for organic treatments and then higher, ζsym and Cs values were similar, while πFT and πIP were opposite being higher then lower. Dry mass production with organic fertilizer was higher than or close to the chemical fertilizer treatments in the later growth stage (day 75), though dry mass production with chemical fertilizers was much higher in the early and middle growing stages (days 15 and 45). Increased photosynthesis and dry mass production in later growth stages due to organic fertilizers were associated with increased osmotic concentration in the tissue and the symplastic fraction of the tissue water compartment. These might favor stomatal opening and biochemical activities.

Photosynthetic and water-related physiological characteristics of Periploca sepium in response to changing soil water conditions in a shell sand habitat

This study was performed to observe the effects of water on photosynthesis and water-related physiology in dominant shrubs in shell sand habitats.Four-year-old Periploca sepium seedlings were used as model species.A gradient of 12 water levels was established by artificially supplying the shell sand with water up to saturation and then allowing natural evapotranspiration to occur.The photo synthetic,chlorophyll fluorescence and stem sap flow parameters of P.sepium were measured under a range of water conditions.The different soil water conditions were classified according to the responses of these parameters.(1)With the increase in the relative water content(RWC)of the shell sand,the parameters of leaf photosynthesis,chlorophyll fluorescence and water-related physiology in P.sepium showed significant critical responses.The net photo synthetic rate(Pn),transpiration rate(Tr),instantaneous water use efficiency(WUE),potential water use efficiency(WUEi),maximum photochemical efficiency(Fv/Fm),actual photochemical efficiency(ΦPSII)and daily accumulation of stem sap flow all increased first and then decreased with increasing RWC,but the corresponding water conditions associated with their maximum values were not the same.An RWC of 69.40%was determined to be the optimal water condition for photosynthesis and water-related physiological activity in P.sepium.At an RWC of 36.61%,the mechanism of photosynthetic inhibition in P.sepium changed from stomatal limitation to nonstomatal limitation;this was also the minimum water requirement for maintaining normal photo synthetic processes.An RWC of 50.27%resulted in the highest WUE in P.sepium,indicating that moderate drought stress increased WUE.(2)Based on the quantitative relationship between the photo synthetic parameters of P.sepium and the shell sand water gradient,the soil water availability was classified into 6 water grades.The RWC range for maintaining strong photosynthesis and high WUE in P.sepium was 63.22-69.98%.(3)Gas exchange in P.sepium was inhibited under drought and waterlogging stresses.Under these conditions,the photosynthetic electron transport chain was blocked,and the dissipation of light energy as heat increased,which ultimately led to a decline in photo synthetic productivity;moreover,transpiration and dissipation were aggravated,and water transmission and utilization processes in P.sepium were hindered.A significant negative feedback regulation mechanism in the photosynthetic and water-related physiological processes of P.sepium was observed;this mechanism allowed P.sepium growing in shell sand to be highly adaptable to water stress.

Seasonality of PSII thermostability and water use e ffi ciency of in situ mountainous Norway spruce(Picea abies)

The stability of monocultural,even-aged spruce forests at lower altitudes in Central Europe is seriously threatened by the prospects of global climate change.The thermostability and water use efficiency of their photo synthetic apparatus might play a vital role in their successful acclimation.In this study,photo systemⅡ(PSⅡ)performance(OJIP transient,rapid light curves)and thermostability were analyzed in Norway spruce(Picea abies(L.)Karst.)throughout the growing season of the exceptionally warm year 2018(May-September)in the Western Carpathians,Slovakia.These measurements were accompanied by analysis of pigment concentrations in the needles.In addition,gas-exchange temperature curves were produced weekly from June until September to obtain intrinsic water use efficiencies.At the beginning of the growing season,needles exposed to heat stress showed significantly higher basal fluorescence and lower quantum yield,performance index,critical temperature thresholds of PSII inactivation and nonphotochemical yield in comparison to other months.The overall thermostability(heat-resistance)of PSII peaked in July and August,reflected in the lowest basal fluorescence and the highest quantum yield of PSII,critical temperature thresholds and yield of non-photochemical quenching under heat stress.Additionally,the ratio between chlorophyll and carotenoids was the highest in August and had a positive impact on PSII thermostability.Moreover,the high-temperature intrinsic water use efficiency was significantly higher during July and August than in June.Results show that15-year-old trees of Picea abies at 840 m a.s.l.exhibited acclimative seasonal responses of PSII thermostability and intrinsic water use efficiency during an exceptionally warm year.Our results suggest that mountainous P.abies at lower altitudes can acclimate their photosynthetic apparatus to higher temperatures during summer.

呼伦贝尔草原生长季叶绿素荧光的特征及其对气象条件变化的响应

在2022年生长季内,基于轨道植被观测仪(型号NZD-G1)对呼伦贝尔草原开展了日光诱导叶绿素荧光(Solar-Induce chlorophyll Fluorescence, SIF)的原位观测。相较于归一化植被指数,SIF的低频变化分量同样可以表征植物在生长季内的长势变化,而高频变化可以更清楚地监测植物内光合作用的生理过程,且其高频变化与气象条件密切相关。土壤水含量和SIF的关系是非线性的,当降水持续偏多导致土壤偏涝时,牧草的生理代谢减弱,光合作用减缓(SIF值偏低);当前期降水适量导致土壤湿润,且太阳辐射较强时,SIF值可以持续稳定在较高水平,植物光合作用旺盛,长势良好。而在生长季后期,偏凉的秋雨会显著降低植物的光合作用,且后期难以恢复。

微咸水灌溉下冬枣光合生理性状与光响应模型研究

【目的】研究不同灌溉水矿化度对冬枣光合生理性状的影响。【方法】基于大田试验,研究1g/L(S1)、2 g/L(S2)、3 g/L(S3)、4 g/L(S4)和5 g/L(S5)灌溉水矿化度下的土壤水盐分布规律与冬枣光合生理响应机制。【结果】叶片净光合速率、蒸腾速率和气孔导度均在3 g/L处理或4 g/L处理下达到峰值;与其他处理相比,4 g/L处理与5g/L处理灌溉水矿化度下的叶片叶绿素量、最大荧光效率和最大量子效率显著降低;与1g/L处理相比,3 g/L处理与4 g/L处理下的冬枣表观量子效率、最大净光合速率、光补偿点、暗呼吸速率显著升高,而光饱和点显著降低,3~4 g/L灌溉水矿化度虽然减小了冬枣的可利用光强范围,但提高了叶片的生理活性。【结论】在果实膨大期,灌溉水矿化度为1~3 g/L时,气孔限制是影响冬枣光合作用的主要因素,而当灌溉水矿化度超过4 g/L时,非气孔限制因素是导致叶片光能利用效率降低的主要原因。综合考虑土壤生态环境与冬枣生理过程,推荐采用3g/L矿化度的微咸水对冬枣进行灌溉。

水钾复合处理对越夏基质培黄瓜秧苗形态及生理指标的影响

黄瓜(Cucumis sativus L.)是设施农业中种植面积最大的蔬菜种类之一,同时也是耗水量较大的蔬菜作物,世界范围的干旱问题日趋严重,水分的缺失使得大量的肥料不能被植物吸收利用,因而植株会出现各种缺肥症状。合理的水肥供应是作物高产高效的生产基础。本试验研究了3种灌溉下限(W60:基质田间持水量的60%;W70:基质田间持水量的70%;W80:基质田间持水量的80%)和3种施钾量(K0:0 mmol/L;K4:4 mmol/L;K8:8 mmol/L)对夏季高温期温室内种植的黄瓜营养生长期的生长发育、叶片光合特性、植株根系指标和植株生物量的影响。结果表明:W70K8处理更好的促进了黄瓜的生长,使其株高、茎粗、叶片数、节间长度和叶面积生长指标显著的增加。W70K8处理可以显著提高黄瓜叶片的叶绿素含量,叶绿素a、叶绿素b和类胡萝卜素含量均为最高。W70K8处理的黄瓜叶片净光合速率最高,为18.36μmol/(m^(2)·s)。W70K8处理显著促进了黄瓜的根系活力,根系活力为88.24μg TTF/(g·h)。综上,W70K8处理可以提高基质栽培黄瓜营养生长期的叶绿素含量、净光合速率、和各项生长指标,促进黄瓜生长发育。试验表明,在夏季设施基质栽培黄瓜营养生长期的适宜水钾耦合模式为:灌溉下限为基质田间持水量的70%,施钾浓度为8 mmol/L。

不同氮磷钾配比水溶肥对生姜光合生理特性及产量的影响

以“冀姜5号”为试材,在生姜小培土至根茎膨大期施用高氮型(T_(1),N-P-K=30-10-20)、高钾型(T_(2),N-P-K=15-6-30)和高磷型(T_(3),N-P-K=10-30-20)3种水溶肥处理,研究不同水溶肥对生姜生长、光合生理特性以及产量的影响。结果表明,各处理中以T_(2)处理茎粗、分枝数和叶面积最高,株高与其他处理差异不显著。与常规施肥(CK)相比,T_(2)处理显著提高了生姜叶片叶绿素含量、净光合速率、气孔导度和蒸腾速率,叶片蔗糖合成酶、谷氨酰胺合成酶和硝酸还原酶活性明显升高,单株根茎鲜重和产量显著增加。与CK相比,T_(1)处理生姜株高显著增加,茎粗显著降低,净光合速率显著提高,叶绿素含量和碳氮代谢相关酶活性与CK无显著差异,产量显著低于T_(2)处理,与CK差异不显著。T_(3)处理各生长指标及光合生理指标均与CK无显著差异,产量显著低于T_(2)和T_(1)处理,与CK差异不显著。因此,在小培土至根茎膨大期,建议施用高钾型水溶肥(N-P-K=15-6-30),能够有效促进生姜生长,提高叶片叶绿素含量、光合效率及碳氮代谢相关酶活性,有利于生姜增产。