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Effect of Different Culture Conditions on Sporulation Quantity of Phytophthora capsici in Capsicum annuum L. var. dactylus M. in Xunhua 被引量:1
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作者 侯全刚 《Plant Diseases and Pests》 CAS 2011年第4期27-29,74,共4页
[Objective] The paper was to explore the effect of different culture conditions on sporulation quantity of Phytophthora capsici in Capsicum annuum L.var.dactylus M.in Xunhua.[Method] The effects of light hours,pH,medi... [Objective] The paper was to explore the effect of different culture conditions on sporulation quantity of Phytophthora capsici in Capsicum annuum L.var.dactylus M.in Xunhua.[Method] The effects of light hours,pH,medium and temperature on sporulation quantity during isolation and culture process of P.capsici were studied.[Result] The sporulation quantity of P.capsici under the conditions of 24 h/day light,pH 7.0,potato medium(PSA) and 30 ℃ was the largest,and pH,basal medium and temperature had greater impact on sporulation quantity.[Conclusion]The study laid foundation for the study on natural incidence condition of C.annuum in Xunhua. 展开更多
关键词 Capsicum annuum L. var. dactylus M. phytophthora capsici Culture condition China
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辣椒疫霉(Phytophthora capsici Leonian)不同发育阶段对嘧菌酯的敏感性研究 被引量:9
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作者 钱忠海 陈长军 +1 位作者 王建新 周明国 《植物病理学报》 CAS CSCD 北大核心 2006年第4期322-327,共6页
细胞色素bc1位点抑制剂包含作用于线粒体内膜外壁CoQ氧化位点抑制剂(QoIs)和作用于线粒体内膜内壁CoQ的还原位点抑制剂(QiIs)。从未使用过QoIs和QiIs的江苏省南京市和安徽省和县随机采集分离获得48个辣椒疫霉单游动孢子囊菌株,并... 细胞色素bc1位点抑制剂包含作用于线粒体内膜外壁CoQ氧化位点抑制剂(QoIs)和作用于线粒体内膜内壁CoQ的还原位点抑制剂(QiIs)。从未使用过QoIs和QiIs的江苏省南京市和安徽省和县随机采集分离获得48个辣椒疫霉单游动孢子囊菌株,并测定了它们不同发育阶段对嘧菌酯的敏感性。结果表明,辣椒疫霉菌丝生长对嘧菌酯的敏感性最低,EC50值范围在1.225~86.100μg/mL,平均值为(21.041±14.397)μg/mL,其活性低于甲霜灵47.7倍;游动孢子囊形成和萌发对嘧菌酯的敏感性较高,EC50值范围分别在0.006~0.996μg/mL和0.053~0.334μg/mL,平均值分别为(0.161±0.126)μg/mL和(0.155±0.023)μg/mL,其活性高于甲霜灵516.8倍;游动孢子萌发对嘧菌酯的敏感性最高,EC。值范围在(0.018-0.111)μg/mL,平均值为(0.057±0.011)μg/mL,活性是甲霜灵的142.4倍。离体生物测定结果表明,水杨肟酸对嘧菌酯抑制菌丝生长的增效作用最强,对游动孢子囊形成的增效作用次之,而对游动孢子囊和游动孢子萌发过程的增效作用则不明显。 展开更多
关键词 辣椒疫霉 嘧菌酯 敏感性基线 增效作用
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南瓜疫病菌(Phytophthora capsici)的生物学特性初探 被引量:6
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作者 张俊华 贾文香 +3 位作者 刘学敏 文景芝 张明厚 周进华 《东北农业大学学报》 CAS CSCD 1998年第1期39-42,共4页
以南瓜疫病菌株PC-1为研究对象,分析其生物学特性。结果表明,不同培养基对菌丝生长影响不同,菌丝在葫萝卜琼脂(CA)培养基上生长要好于燕麦片琼脂(OMA)培养基。孢子囊的产生需要有水分,同时也受温度、光照、培养基及菌... 以南瓜疫病菌株PC-1为研究对象,分析其生物学特性。结果表明,不同培养基对菌丝生长影响不同,菌丝在葫萝卜琼脂(CA)培养基上生长要好于燕麦片琼脂(OMA)培养基。孢子囊的产生需要有水分,同时也受温度、光照、培养基及菌龄的影响。在一定温度范围内,温度升高,孢子囊产生的速度加快且数量多,光照有利于孢子囊的产生,10℃以下无论有无光照均不产生孢子囊。 展开更多
关键词 南瓜 疫病菌 生物学特性 病害
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辣椒疫霉(Phytophthora capsici)果胶甲基酯酶Pcpme3基因真核表达及功能研究 被引量:3
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作者 贾永健 冯宝珍 +2 位作者 李培谦 张修国 付红波 《中国农业科学》 CAS CSCD 北大核心 2009年第6期1988-1993,共6页
【目的】对辣椒疫霉果胶甲基酯酶Pcpme3基因进行真核表达,制备其特异性抗血清,为用Westernblot检测该基因在辣椒疫霉致病过程中的功能奠定基础。【方法】利用TR-PCR分离鉴定Pcpme3的成熟肽片段,克隆于pPIC9K载体。将获得的表达载体转化... 【目的】对辣椒疫霉果胶甲基酯酶Pcpme3基因进行真核表达,制备其特异性抗血清,为用Westernblot检测该基因在辣椒疫霉致病过程中的功能奠定基础。【方法】利用TR-PCR分离鉴定Pcpme3的成熟肽片段,克隆于pPIC9K载体。将获得的表达载体转化毕赤酵母GS115,甲醇诱导表达,对表达产物进行SDS-PAGE分析和免疫家兔制备特异性抗体。【结果】酵母转化子经MD、MM平板筛选和G418抗生素筛选与PCR鉴定,获得Mut+/His+表型高拷贝重组转化子,经1%甲醇诱导后,SDS-PAGE检测发酵上清液,检测出43kD的特异蛋白。重组蛋白免疫家兔制备特异抗血清,Westernblot检测该基因在游动孢子侵染辣椒叶片中的表达,随着病情加重,该基因的表达逐渐增强,从而证明该基因参与了病菌的侵染致病过程。【结论】利用真核表达获得的蛋白制成特异性抗体可以有效地检测Pcpme3在辣椒疫霉侵染寄主过程中的功能特性。 展开更多
关键词 辣椒疫霉 果胶甲基酯酶 真核表达 抗血清
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四川茄子对绵疫病菌Phytophthora capsici的抗性鉴定与评价 被引量:6
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作者 席亚东 乔昕 +3 位作者 房超 吴婕 韩帅 张河庆 《北方园艺》 CAS 北大核心 2020年第14期8-17,共10页
绵疫病是近5年四川茄子田间和储藏期最具毁灭性的病害,其病原菌Phytophthora capsici对植株和果实均具强致病性。以四川省市售的48个商品种为靶标,研究其对绵疫病主要病原菌Phytophthora capsici的抗性鉴定和评价。结果表明:排除5号(&qu... 绵疫病是近5年四川茄子田间和储藏期最具毁灭性的病害,其病原菌Phytophthora capsici对植株和果实均具强致病性。以四川省市售的48个商品种为靶标,研究其对绵疫病主要病原菌Phytophthora capsici的抗性鉴定和评价。结果表明:排除5号("世纪骄子")和47号("龙F1")出苗差外,实际参鉴的46个品种中,不同品种茄子植株在接种7 d后的病情指数最高为96.51("阳春三月茄"),最低为12.09("竹丝茄");聚类分析中,"春秋墨茄"和"竹丝茄"的病株率和病情指数均聚在一起,室内植株抗性表现为中抗,其它2个品种表现为感病、42个品种表现为高感;对46个品种的病情指数和病株率的Kmeans线图进行分析,可分为6类不同发生发展模式,病情指数的Kmeans线图相比病株率的Kmeans图更具规律性和代表性,其中Cluster4类群(包含"春秋墨茄"和"竹丝茄")的植株病情指数Kmeans线图可作为后续筛选抗性品种的模式图。对46个品种的果实进行接种和抗病性鉴定,结果表明46个品种的果实均高感茄子绵疫病菌Phytophthora capsici,未发现果实抗病的品种,包括对植株具有中抗水平的"春秋墨茄"和"竹丝茄",果实均高感由Phytophthora capsici引起的茄子绵疫病。"春秋墨茄"和"竹丝茄"植株对Phytophthora capsici具有一定抗性,而果实却表现高感,可作为后续抗性的组织特异性深入研究。 展开更多
关键词 茄子 茄子绵疫病 辣椒疫霉菌 组织器官抗病特异性 抗病性
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胡椒瘟病菌(Phytophthora capsici)孢子囊诱导及发育过程观察 被引量:2
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作者 高圣风 杨开虎 +5 位作者 陆大倩 刘爱勤 苟亚峰 孙世伟 王政 孟倩倩 《热带作物学报》 CSCD 北大核心 2020年第9期1870-1875,共6页
孢子囊的产生和发育过程是研究胡椒瘟病菌(Phytophthora capsici)及其病害防控的重要基础。本研究分析不同诱导条件下胡椒瘟病菌孢子囊的产生情况,并通过光学显微镜和共聚焦显微镜观察孢子囊及其内部游动孢子的发育过程。结果发现:(1)3... 孢子囊的产生和发育过程是研究胡椒瘟病菌(Phytophthora capsici)及其病害防控的重要基础。本研究分析不同诱导条件下胡椒瘟病菌孢子囊的产生情况,并通过光学显微镜和共聚焦显微镜观察孢子囊及其内部游动孢子的发育过程。结果发现:(1)3种诱导方法均能诱导出大量孢子囊,其中在V8-A平板上光照和抹伤双重诱导法获得的孢子囊数量最多,其次是在V8-A平板上光照诱导法,获得孢子囊数量最少的是V8液体光照诱导法,但仅差异最大的2个处理间达到显著水平;(2)显微镜观察发现,孢子囊由气生菌丝顶端逐步膨大形成,初始为近球形逐渐发育成倒洋梨形,孢子囊成熟后从顶端排出大量游动孢子,偶尔可见孢子囊顶端直接发育出芽管;(3)共聚焦显微镜观察发现,首先孢子囊内部原生质体被膜结构隔裂成大约数十个独立小格,然后在每个格子中积累数倍于细胞核的DNA,最后每份细胞核DNA发育成一个游动孢子。本研究从微观角度揭示P.capsici孢子囊发育外观及内部的形态特征,为胡椒瘟病菌后续致病机制研究和胡椒瘟病田间防控提供技术基础。 展开更多
关键词 胡椒瘟病 辣椒疫霉 孢子囊诱导 发育过程
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辣椒疫霉(Phytophthora capsici)多聚半乳糖醛酸酶PCIPG2N-糖基化突变体的构建与表达分析 被引量:1
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作者 李艳青 夏海波 张修国 《安徽农业大学学报》 CAS CSCD 北大核心 2014年第5期783-788,共6页
为研究辣椒疫霉(Phytophthora capsici)多聚半乳糖醛酸酶PCIPG2 N-糖基化对其酶活性的影响,从基因组文库中分离克隆到Pcipg2基因,利用定点突变技术突变3个潜在的糖基化位点(N34,N76,N137);构建并表达N-糖基化突变蛋白,并对突变蛋白进行... 为研究辣椒疫霉(Phytophthora capsici)多聚半乳糖醛酸酶PCIPG2 N-糖基化对其酶活性的影响,从基因组文库中分离克隆到Pcipg2基因,利用定点突变技术突变3个潜在的糖基化位点(N34,N76,N137);构建并表达N-糖基化突变蛋白,并对突变蛋白进行温度和缓冲液体系处理检测其活性。结果发现Pcipg2基因在辣椒疫霉侵染寄主的前期表达并起重要作用。PCIPG2蛋白在30°C,pH5.0(P<0.05)的缓冲液环境下活性最高。单个的Pcipg2糖基化位点N34、N76、N137对PCIPG2的致病性起正调控作用,而3个糖基化位点相互协调的功能抑制PCIPG2的活性,在PCIPG2表达致病过程中起负调控。N-糖基化在PCIPG2酶活性上起直接作用,使得PCIPG2酶在较低水平上保持较高的稳定性。 展开更多
关键词 辣椒疫霉 多聚半乳糖醛酸酶PCIPG2 N-糖基化 活性
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Induction and Characterization of Laboratory Mutants of Phytophthora capsici Resistant to Dimethomorph and Flumorph 被引量:3
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作者 YUAN Shan-kui LIU Xi-li +3 位作者 GU Bao-gen DONG Jin JIANG Hui SI Nai-guo 《Agricultural Sciences in China》 CAS CSCD 2005年第10期752-759,共8页
Laboratory studies were conducted to evaluate the risk of Phytophthora capsici developing resistance to two morphlines, dimethomorph and flumorph. Metalaxyl, the well-known high risk of resistance fungicides, was used... Laboratory studies were conducted to evaluate the risk of Phytophthora capsici developing resistance to two morphlines, dimethomorph and flumorph. Metalaxyl, the well-known high risk of resistance fungicides, was used as reference fungicide. Resistant mutants for the three fungicides were isolated by treating mycelium with ultraviolet radiation. Metalaxyl-resistant mutants were obtained with high frequency and exhibited high level of resistance with factors more than 100 folds, while mutation frequency for dimethomorph-resistance was relatively low and the resistance factors ranged from 3.0 to 13.9 folds. Most dimethomorph-resistant mutants decreased in hyphal growth rate and the spoulation ability, which have a large impact upon the epidemic development of dimethomorph-resistant populations. These results suggested that the risk of resistant pathogen population was much lower for dimethomorph than for metalaxyl. Both the frequency of developing resistance and level of resistance (resistance factors = 1.8-14.6) to dimethomorph were similar to those of its structure analogue flumorh. Moreover, the cross-resistance were found between them, which suggested the risks of developing resistance to dimethomorph and flumorph in the pathogen were very closely related. As P. capsici can potentially develop resistance to dimethomorph and flumorph, and oomycetes usually have the high risk to develop resistance to fungicides, appropriate management against resistance development should be taken. 展开更多
关键词 phytophthora capsici Resistance DIMETHOMORPH FLUMORPH METALAXYL
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Active changes of lignification-related enzymes in pepper response to Glomus intraradices and/or Phytophthora capsici 被引量:2
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作者 郑虎哲 崔春兰 +3 位作者 张玉廷 王丹 荆宇 KIM Kil Yong 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE EI CAS CSCD 2005年第8期778-786,共9页
The activities of enzymes responsible for lignification in pepper, pre-inoculation with arbuscular mycorrhizal (AM) fungus of Glomus intraradices and/or infection with pathogenic strain of Phytophthora capsici, and th... The activities of enzymes responsible for lignification in pepper, pre-inoculation with arbuscular mycorrhizal (AM) fungus of Glomus intraradices and/or infection with pathogenic strain of Phytophthora capsici, and the biological control effect of G. intraradices on Phytophthora blight in pepper were investigated. The experiment was carried out with four treatments: (1) plants pre-inoculated with G. intraradices (Gi), (2) plants pre-inoculated with G. intraradices and then infected with P. capsici (Gi+Pc), (3) plants infected with P. capsici (Pc), and (4) plants without any of the two microorganisms (C). Mycorrhizal coloni-zation rate was reduced by about 10% in pathogen challenged plants. Root mortality caused by infection of P. capsici was com-pletely eliminated by pre-inoculation with antagonistic G. intraradices. On the ninth day after pathogen infection, Peroxidase (POD) activity increased by 116.9% in Pc-treated roots but by only 21.2% in Gi+Pc-treated roots, compared with the control, respectively. Polyphenol oxidase (PPO) and Phenylalanine ammonia-lyase (PAL) activities gradually increased during the first 3 d and dramatically decreased in Pc-treated roots but slightly decreased in Gi+Pc-treated roots, respectively. On the ninth day after pathogen infection, PPO and PAL decreased by 62.8% and 73.9% in Pc-treated roots but by only 19.8% and 19.5% in Gi+Pc-treated roots, compared with the control, respectively. Three major POD isozymes (45 000, 53 000 and 114 000) were present in Pc-treated roots, while two major bands (53 000 and 114 000) and one minor band (45 000) were present in spectra of Gi+Pc-treated roots, the 45 000 POD isozyme was significantly suppressed by G. intraradices, suggesting that the 45 000 POD isozyme was induced by the pathogen infection but not induced by the antagonistic G. intraradices. A 60 000 PPO isozyme was induced in Pc-treated roots but not induced in Gi+Pc-treated roots. All these results showed the inoculation of antagonistic G. intraradices alleviates root mortality, activates changes of lignification-related enzymes and induces some of the isozymes in pepper plants infected by P. capsici. The results suggested that G. intraradices is a potentially effective protection agent against P. capsici. 展开更多
关键词 Arbuscular mycorrhizal (AM) fungus Glomus intraradices phytophthora capsici Peroxidase (POD) Polyphenol oxidase (PPO) Phenylalanine ammonia-lyase (PAL)
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南瓜疫病菌(Phytophthora capsici)生理分化研究进展
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作者 付洪冰 张俊华 +1 位作者 刘琦 崔崇士 《东北农业大学学报》 CAS CSCD 2008年第2期271-274,共4页
文章综述了南瓜疫病菌生理分化的研究进展,包括传统的鉴别寄主技术到分子标记技术的应用,展望今后南瓜疫病菌的主要研究方向与前景,为今后南瓜抗疫病育种研究奠定理论和应用基础。
关键词 南瓜疫病菌 生理分化 分子标记
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Inhibitory Effect of Extracts from Helianthus tuberosus Leaves against Phytophthora capsici and Pot Verification Test
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作者 Li Yi Wang Lihui +2 位作者 Han Rui Fu Yingkun Tan Long 《Plant Diseases and Pests》 CAS 2015年第2期19-22,共4页
By growth rate method, the inhibitory effects of five solvent extracts from Helianthus tuberosus leaves against Phytophthora capsici were studied in the test. The results showed that different solvent extracts all had... By growth rate method, the inhibitory effects of five solvent extracts from Helianthus tuberosus leaves against Phytophthora capsici were studied in the test. The results showed that different solvent extracts all had inhibitory effect against P. capsici, while 12.5 mg/mL of extracts from H. tuberosus leaves with petroleum ether and ethyl acetate as solvents had the highest inhibitory effect against P. capsici, reaching 100%. In case of various solvent extracts with different concentration gradients, ethyl acetate extract had the most significant inhibitory effect; when the concentration was 5 mg/mL, the inhibitory effect of ethyl acetate extract had reached 100% ; when the concentration reduced to 2.5 mg/mL, the inhibitory effect was still (27.91 ±2. 076) %, significantly higher than that of other solvent extracts at the same concentration. The 50 times dilution of ethyl acetate extract from H. tuberosus leaves was selected for pot test against pepper blight. , and the results showed that its control effect against pepper blight reached 100.00%, superior than that of chemical agent 25% metalaxyl WP 400 times dilution. 展开更多
关键词 Helianthus tuberosus EXTRACT phytophthora capsici Helianthus tuberosus Inhibitory effect Petroleum ether Ethyl acetate
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Genetic Diversity and Phylogenetic Relationships among Phytophthora capsici Isolates from Guizhou Province by RAPD
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作者 Ying WANG Shu WANG +4 位作者 Yuqiao ZHAO Zhun XIANG Hongzao HE Bixia XIANG Chao SUN 《Agricultural Biotechnology》 CAS 2014年第2期44-46,共3页
Ten random primers with clear amplification profile, significant and stable main band were screened from RAPD (Random Amplified Polymorphie DNAs) primers to analyze the genetic diversity among eight Phytophthora cap... Ten random primers with clear amplification profile, significant and stable main band were screened from RAPD (Random Amplified Polymorphie DNAs) primers to analyze the genetic diversity among eight Phytophthora capsici isolates from Huaxi District, Wudang District and Kaiyang County of Guiyang City, and Zunyi County, Suiyang County and Luodian County of Zunyi City in Guizhou Province. A total of 70 DNA fingerprints were obtained, including 57 polymorphic bands, with a polymorphic percentage of 81.43%, suggesting abundant genetic diversity among experimental Phytophthora capsici isolates. According to the ampli- fied DNA fingerprint profiles, using genetic similarity coefficient 0.5 as the threshold, experimental Phytophthora capsici isolates were clustered into three genetic categories by UPGMA cluster analysis. The analysis result indicated that there was no direct correlation between the genetic similarity and cultivation areas of vari- ous Phytophthora caosici isolates. 展开更多
关键词 phytophthora capsici Genetic diversity RAPD DNA fingerprint
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Spread of <i>Phytophthora capsici</i>in Black Pepper (<i>Piper nigrum</i>) in Vietnam 被引量:4
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作者 Van Long Nguyen 《Engineering(科研)》 2015年第8期506-513,共8页
Black pepper is the one of most important export products in Vietnam. As the largest exporter, Vietnam’s pepper commodities account for 58% of total worldwide exporters. However, Vietnam’s pepper production is deali... Black pepper is the one of most important export products in Vietnam. As the largest exporter, Vietnam’s pepper commodities account for 58% of total worldwide exporters. However, Vietnam’s pepper production is dealing with disease problems, especially foot rot/quick death infected by Phytophthora capsici. The disease results in serious and rapid spread and infection in Vietnam, with yearly reduction of about 2% of total pepper area. Disease management is recently challenging scientists and producers. Investigating characteristics of Phytophthora capsici and causes, therefore, play a significant role in treatment. This paper has indicated three main causes, which contribute to serious infection and outbreak of Phytophthora capsici;they are biological characteristics, climatic condition and cultivation. To control this disease, early detection and prevention are the best ways to manage disease. Finding new varieties, which are Phytophthora capsici tolerance or resistance, is significant in black pepper production worldwide. 展开更多
关键词 Black PEPPER phytophthora capsici Cause Management VIETNAM
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对上海郊区甜椒上6株Phytophthora capsici形态与生理生化性状的研究
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作者 杨芝 《北京农学院学报》 1989年第3期71-76,共6页
作者观察了来自于上海郊区甜椒上的6株P.capsici的菌落形态、孢子囊特征。对6株病菌孢子囊的大小、最高生长温度、对孔雀石绿的敏感性、在含有酪氨酸的培养基上能否产生色素、利用硝态氮和淀粉的能力等生理生化性状进行了测定和研究。
关键词 甜椒 疫病菌 形态 生理生化性状
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辣椒疫霉(Phytophthora capsici)对辣椒的致病力分化研究 被引量:14
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作者 李萍 江涛 +2 位作者 高智谋 戚仁德 汪涛 《植物病理学报》 CAS CSCD 北大核心 2012年第4期431-435,共5页
辣椒疫霉(Phytophthoracapsici Leonian)是具有重大危害性的病原卵菌,其寄主范围较广,可引致辣椒、番茄、茄子、黄瓜、南瓜等多种重要蔬菜作物的疫病。由辣椒疫霉引起的辣椒疫病是一种毁灭性病害,在世界各地的辣椒种植区普遍发生... 辣椒疫霉(Phytophthoracapsici Leonian)是具有重大危害性的病原卵菌,其寄主范围较广,可引致辣椒、番茄、茄子、黄瓜、南瓜等多种重要蔬菜作物的疫病。由辣椒疫霉引起的辣椒疫病是一种毁灭性病害,在世界各地的辣椒种植区普遍发生,我国尤以江苏、浙江、安徽、上海等长江中下游地区发生严重。 展开更多
关键词 辣椒疫霉 致病力分化 长江中下游地区 辣椒疫病 毁灭性病害 寄主范围 蔬菜作物 种植区
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A Phytophthora capsici RXLR Effector Targets and Inhibits a Plant,PPlase to Suppress Endoplasmic Reticulum-Mediated Immunity 被引量:21
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作者 Guangjin Fan Yang Yang +5 位作者 Tingting Li Wenqin Lu Yu DU Xiaoyu Qiang Qujiang Wen Weixing Shan 《Molecular Plant》 SCIE CAS CSCD 2018年第8期1067-1083,共17页
Phytophthora pathogens secrete a large arsenal of effectors that manipulate host processes to create an environment conducive to pathogen colonization. However, the underlying mechanisms by which Phytophthora effector... Phytophthora pathogens secrete a large arsenal of effectors that manipulate host processes to create an environment conducive to pathogen colonization. However, the underlying mechanisms by which Phytophthora effectors manipulate host plant cells still remain largely unclear. In this study, we report that PcAvr3a12, a Phytophthera capsici RXLR effector and a member of the Avr3a effector family, suppresses plant immunity by targeting and inhibiting host plant peptidyl-prolyl cis-trans isomerase (PPlase). Overexpression of PcAvr3a 12 in Arabidopsis thaliana enhanced plant susceptibility to P. capsici. FKBP15-2, an endoplasmic reticulum (ER)-Iocalized protein, was identified as a host target of PcAvr3a12 during early P. capsici infection. Analyses of A. thaliana T-DNA insertion mutant (fkbp15-2), RNAi, and overexpression lines consistently showed that FKBP15-2 positively regulates plant immunity in response to Phytophthora infection. FKBP15-2 possesses PPlase activity essential for its contribution to immunity but is directly suppressed by PcAvr3a12. Interestingly, we found that FKBP15-2 is involved in ER stress sensing and is required for ER stress-mediated plant immunity. Taken together, these results suggest that P. capsici deploys an RXLR effector, PcAvr3a12, to facilitate infection by targeting and suppressing a novel ER-Iocalized PPlase, FKBP15-2, which is required for ER stress-mediated plant immunity. 展开更多
关键词 RXLR effector Avr3a FKBP ER stress IMMUNITY phytophthora capsici
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辣椒疫霉菌(Phytophthora capsici)实时荧光定量检测方法的研究 被引量:1
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作者 王淑芳 马桂珍 +4 位作者 暴增海 李世东 陈月 钱媛媛 尹璐 《作物杂志》 CSCD 北大核心 2012年第5期30-35,共6页
为建立快速准确的检测土壤中辣椒疫霉菌(Phytophthora capsici Leonian)的实时荧光定量体系的方法,根据辣椒疫霉菌保守的ITS序列设计的特异性荧光实时定量PCR的引物,通过对保守序列构建克隆文库,筛选阳性克隆子,制备用于实时荧光定量体... 为建立快速准确的检测土壤中辣椒疫霉菌(Phytophthora capsici Leonian)的实时荧光定量体系的方法,根据辣椒疫霉菌保守的ITS序列设计的特异性荧光实时定量PCR的引物,通过对保守序列构建克隆文库,筛选阳性克隆子,制备用于实时荧光定量体系的标准品,从而构建优良的标准曲线。利用构建的标准曲线和优化的实时荧光定量体系对人工接种含梯度浓度的辣椒疫霉菌的土壤样品进行实时荧光定量PCR检测。辣椒疫霉菌荧光实时定量PCR检测体系的标准曲线的相关系数为R2=0.980,斜率为-3.295,扩增效率为101.1%,线性方程为Y=-3.295X+44.484,标准品的检测下限为1.000×102拷贝,带菌土壤的检测下限为1.236×103拷贝,约为4.887pg基因组DNA。经过土壤样品试验,得出标准曲线相关系数为0.9845,表明所建立的辣椒疫霉菌实时荧光定量PCR检测方法合理有效。 展开更多
关键词 辣椒疫霉菌 快速检测 实时荧光定量 PCR
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球毛壳菌小麦秸秆发酵产物抑制辣椒疫霉的稳定性及机制
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作者 廖宏娟 谭佳思 +4 位作者 张志斌 余璟蓉 张馨月 江玉梅 朱笃 《中国农业科学》 CAS CSCD 北大核心 2024年第3期500-513,共14页
【目的】由辣椒疫霉(Phytophthora capsici)侵染引起的疫病给辣椒等作物产业带来了巨大的经济损失。论文旨在明确球毛壳菌(Chaetomium globosum)代谢产物抑制辣椒疫霉的稳定性和抑菌机制,为研究与开发辣椒疫霉的微生物源抑菌剂提供参考... 【目的】由辣椒疫霉(Phytophthora capsici)侵染引起的疫病给辣椒等作物产业带来了巨大的经济损失。论文旨在明确球毛壳菌(Chaetomium globosum)代谢产物抑制辣椒疫霉的稳定性和抑菌机制,为研究与开发辣椒疫霉的微生物源抑菌剂提供参考。【方法】将球毛壳菌发酵粗提物分别在不同温度(40—121℃)、pH(1—13)、光照时间(0—12 d)和储存时间(0—60 d)条件下进行处理,采用菌丝生长抑制法测定经不同处理后粗提物对辣椒疫霉的抑制率,以探究粗提物抑制辣椒疫霉的热、酸碱、光和时间稳定性。利用光学显微镜观察粗提物对辣椒疫霉菌丝形态的影响,通过多种生理生化试验探究粗提物作用于辣椒疫霉12—72 h后,对辣椒疫霉细胞壁、细胞膜、活性氧代谢、蛋白质含量、还原糖含量和致病力的影响。【结果】在所设置的处理范围内,球毛壳菌发酵粗提物(1 mg·mL^(-1))经不同光照时间和储存时间处理后对辣椒疫霉的抑制率没有显著降低,抑制率维持在93%左右;粗提物在40—70℃的热处理范围内对辣椒疫霉的抑制率没有显著降低,70℃以上的热处理会显著降低粗提物对辣椒疫霉的抑制率,但抑制率不低于70%;粗提物在pH 1—5和9—13的酸碱处理范围内会显著降低对辣椒疫霉的抑制率,但抑制率也不低于70%。粗提物处理影响辣椒疫霉菌丝形态,使菌丝发生严重的扭曲皱缩现象,并影响活性氧代谢,使菌丝中的活性氧大量积累。辣椒疫霉经粗提物处理12—72 h后,其培养液中的碱性磷酸酶活性、β-葡萄糖苷酶活性、核酸和蛋白质含量均显著升高,且其菌丝中的丙二醛和过氧化氢含量显著增加。辣椒疫霉菌丝中的过氧化氢酶活性、可溶性蛋白和还原糖含量则在粗提物处理12—72 h内显著降低,而超氧化物歧化酶、过氧化物酶、多聚半乳糖醛酸酶和β-葡萄糖苷酶活性则仅在一定时间显著降低。【结论】在本试验所设置的处理范围内,球毛壳菌的小麦秸秆发酵粗提物抑制辣椒疫霉的效果不受光照及储存时间的影响,但超过70℃的热处理和pH 1—5和9—13的酸碱处理会显著降低粗提物对辣椒疫霉的抑制效果。此外,该粗提物通过改变菌丝形态,破坏细胞壁和细胞膜,引发细胞内物质泄漏,降低菌丝中蛋白质和还原糖含量,抑制抗氧化酶活性,干扰活性氧代谢使活性氧大量积累从而抑制辣椒疫霉。 展开更多
关键词 球毛壳菌 辣椒疫霉 抑制率 抑制机制 抑制稳定性
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辣椒CaBBX2基因的克隆与表达分析
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作者 柳佳欣 吴丹 +5 位作者 陶思政 罗英 杨凤 余婷 杨有新 周勇 《核农学报》 CAS CSCD 北大核心 2024年第9期1671-1681,共11页
为探究BBX转录因子在辣椒抵御疫霉菌侵染过程中的功能,本研究以辣椒品种007EA为材料,对辣椒BBX转录因子CaBBX2基因进行克隆和表达分析,并对其编码蛋白进行亚细胞定位和生物信息学分析。聚合酶链式反应(PCR)扩增和测序结果表明,CaBBX2的... 为探究BBX转录因子在辣椒抵御疫霉菌侵染过程中的功能,本研究以辣椒品种007EA为材料,对辣椒BBX转录因子CaBBX2基因进行克隆和表达分析,并对其编码蛋白进行亚细胞定位和生物信息学分析。聚合酶链式反应(PCR)扩增和测序结果表明,CaBBX2的编码区序列长为639 bp,编码212个氨基酸。蛋白理化分析结果显示,CaBBX2蛋白分子量为23.5 kDa,理论等电点(pI)值为6.17,总平均亲水系数为-0.559,表明CaBBX2为亲水性蛋白。蛋白质结构预测及蛋白序列比对结果显示,CaBBX2含有2个B-box保守结构域。进化树分析结果显示,CaBBX2蛋白属于Ⅳ亚组的BBX蛋白,与拟南芥AtBBX18和AtBBX19蛋白亲缘关系最近。启动子分析结果显示,CaBBX2启动子序列中含有与胁迫、激素和光响应有关的顺式作用元件。亚细胞定位结果显示,CaBBX2同时定位于细胞质和细胞核。组织特异性表达发现,CaBBX2基因在辣椒不同组织中均有表达,其中在叶中的表达量最高,在果皮中的表达量最低。采用实时荧光定量PCR(qRT-PCR)分析CaBBX2在疫霉菌接种及茉莉酸甲酯(MeJA)处理后表达量的变化,发现接种疫病后CaBBX2的表达量明显上升,MeJA处理后CaBBX2表达量呈现明显下降趋势。上述结果表明CaBBX2可能参与激素调控响应辣椒疫病胁迫的应答过程,可为辣椒抗病分子育种提供重要的候选基因。 展开更多
关键词 辣椒 BBX转录因子 疫霉菌 表达分析
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辣椒抗疫病种质资源筛选
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作者 张婧文 张卓 +3 位作者 赵志祥 张玉涵 周倩 刘勇 《湖南农业科学》 2024年第4期67-71,共5页
由辣椒疫霉菌(Phytophthora capsici)侵染引起的辣椒疫病是一种危害严重的土传病害,为了筛选抗辣椒疫病的辣椒种质资源,试验以31份辣椒种质资源为材料,在温室栽培条件下,采用灌根法接种辣椒疫霉菌,鉴定各材料对辣椒疫病的抗性级别,鉴定... 由辣椒疫霉菌(Phytophthora capsici)侵染引起的辣椒疫病是一种危害严重的土传病害,为了筛选抗辣椒疫病的辣椒种质资源,试验以31份辣椒种质资源为材料,在温室栽培条件下,采用灌根法接种辣椒疫霉菌,鉴定各材料对辣椒疫病的抗性级别,鉴定结果为:不同辣椒种质资源材料之间的抗性有明显差异,病情指数在0~94之间;表现为高抗材料7份、抗病材料2份、中抗材料9份、感病材料10份。 展开更多
关键词 辣椒疫霉菌 辣椒疫病 抗病 种质资源
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