通过模拟人体胃肠道环境,研究植物蛋白发酵乳酸菌植物乳杆菌FJAT-7926(Lactobacillus plantarum FJAT-7926)和干酪乳杆菌FJAT-7928(Lactobacillus casei FJAT-7928)在不同pH值人工胃液和不同胆盐浓度人工肠液下的存活情况。结果表明,pH...通过模拟人体胃肠道环境,研究植物蛋白发酵乳酸菌植物乳杆菌FJAT-7926(Lactobacillus plantarum FJAT-7926)和干酪乳杆菌FJAT-7928(Lactobacillus casei FJAT-7928)在不同pH值人工胃液和不同胆盐浓度人工肠液下的存活情况。结果表明,pH3.0的人工胃液对2株菌的存活影响较小。经4h处理后,2株菌的存活数都在1×108cfu·mL-1以上,存活率分别为56.06%和55.65%。pH2.0的人工胃液对2株菌的影响较大,经2h处理后,2株菌的存活数降至1×105cfu·mL-1,4h后,2株菌存活数分别为3.67×102 cfu·mL-1和2.30×103cfu·mL-1。2株菌对不同胆盐浓度的人工肠液均有较好的耐受性。0.1%胆盐浓度的人工肠液对2株菌的存活基本没影响。随着胆盐浓度的升高,2株菌的存活率有所下降,但都与初始菌数维持在同一数量级上。在中高浓度胆盐(0.3%和0.5%)的人工肠液中处理4h后,植物乳杆菌FJAT-7926的存活率在70%以上,干酪乳杆菌FJAT-7928的存活率在40%以上。可见这2株菌均有较强的胃肠道环境耐受力,符合微生态制剂和乳酸菌发酵功能食品菌种耐受肠胃环境的要求,在植物乳酸菌发酵食品方面具有广阔的应用前景。展开更多
With the rise of organic agriculture,researches on the production of slow-release organic fertilizer from natural organic materials have attracted much attention.Plant protein and peptides have potential applications ...With the rise of organic agriculture,researches on the production of slow-release organic fertilizer from natural organic materials have attracted much attention.Plant protein and peptides have potential applications in agricultural production.The effects of bioactive peptides derived from plant protein on rice seed germination and seedling growth and development were systematically studied using bioassays and greenhouse growth experiments.The feasibility of phytoprotein peptide application in the rice seedling stage was discussed.The results showed that the phytoprotein peptides had no adverse effects on rice,and the application when applied to rice seedlings,phytoprotein peptides within a certain concentration range produced obvious promotion effects on the growth and development of rice seedlings,which were more pronounced in the underground part of the plants.Phytoprotein peptides increased root length and dry matter accumulation and improved the robustness of rice seedlings.A 40 g·mol^(-1)·L^(-1) dose of phytoprotein peptides increased root length,plant fresh weight,plant dry weight,root fresh weight and root dry weight by 59.24%,20.14%,26.32%,51.57%and 81.70%,respectively compared to the control.In addition,the phytoprotein peptides significantly increased the chlorophyll content,root activity and nitrate reductase activity of rice seedlings,as well as superoxide dismutase,peroxidase,catalase andβ-1,3-glucanase activity in rice leaves,which could improve stress resistance.Plant peptides were simple to produce and cost-effective.Reasonable development and excavation of plant protein and protein hydrolysate peptide in China's agricultural application had a good practical basis and value significance.展开更多
文摘通过模拟人体胃肠道环境,研究植物蛋白发酵乳酸菌植物乳杆菌FJAT-7926(Lactobacillus plantarum FJAT-7926)和干酪乳杆菌FJAT-7928(Lactobacillus casei FJAT-7928)在不同pH值人工胃液和不同胆盐浓度人工肠液下的存活情况。结果表明,pH3.0的人工胃液对2株菌的存活影响较小。经4h处理后,2株菌的存活数都在1×108cfu·mL-1以上,存活率分别为56.06%和55.65%。pH2.0的人工胃液对2株菌的影响较大,经2h处理后,2株菌的存活数降至1×105cfu·mL-1,4h后,2株菌存活数分别为3.67×102 cfu·mL-1和2.30×103cfu·mL-1。2株菌对不同胆盐浓度的人工肠液均有较好的耐受性。0.1%胆盐浓度的人工肠液对2株菌的存活基本没影响。随着胆盐浓度的升高,2株菌的存活率有所下降,但都与初始菌数维持在同一数量级上。在中高浓度胆盐(0.3%和0.5%)的人工肠液中处理4h后,植物乳杆菌FJAT-7926的存活率在70%以上,干酪乳杆菌FJAT-7928的存活率在40%以上。可见这2株菌均有较强的胃肠道环境耐受力,符合微生态制剂和乳酸菌发酵功能食品菌种耐受肠胃环境的要求,在植物乳酸菌发酵食品方面具有广阔的应用前景。
基金Supported by the National Key Research and Development Program of China and the Project of Technologies and Product Development of Synergist to Chemical Pesticides(2016YFD0200507)。
文摘With the rise of organic agriculture,researches on the production of slow-release organic fertilizer from natural organic materials have attracted much attention.Plant protein and peptides have potential applications in agricultural production.The effects of bioactive peptides derived from plant protein on rice seed germination and seedling growth and development were systematically studied using bioassays and greenhouse growth experiments.The feasibility of phytoprotein peptide application in the rice seedling stage was discussed.The results showed that the phytoprotein peptides had no adverse effects on rice,and the application when applied to rice seedlings,phytoprotein peptides within a certain concentration range produced obvious promotion effects on the growth and development of rice seedlings,which were more pronounced in the underground part of the plants.Phytoprotein peptides increased root length and dry matter accumulation and improved the robustness of rice seedlings.A 40 g·mol^(-1)·L^(-1) dose of phytoprotein peptides increased root length,plant fresh weight,plant dry weight,root fresh weight and root dry weight by 59.24%,20.14%,26.32%,51.57%and 81.70%,respectively compared to the control.In addition,the phytoprotein peptides significantly increased the chlorophyll content,root activity and nitrate reductase activity of rice seedlings,as well as superoxide dismutase,peroxidase,catalase andβ-1,3-glucanase activity in rice leaves,which could improve stress resistance.Plant peptides were simple to produce and cost-effective.Reasonable development and excavation of plant protein and protein hydrolysate peptide in China's agricultural application had a good practical basis and value significance.
