The low yield and poor fuel properties of bio-oil have made the pyrolysis production process uneconomic and also limited bio-oil usage.Proper manipulation of key pyrolysis variables is paramount in order to produce hi...The low yield and poor fuel properties of bio-oil have made the pyrolysis production process uneconomic and also limited bio-oil usage.Proper manipulation of key pyrolysis variables is paramount in order to produce high-quality bio-oil that requires less upgrading.In this research,the pyrolysis of pig hair was carried out in a fixed-bed reactor using a calcium oxide catalyst derived from calcination of turtle shells.In the pyrolysis process,the influence of three variables-temperature,heating rate and catalyst weight-on two responses-bio-oil yield and its higher heating value(HHV)-were investigated using Response Surface Methodology.A second-order regression-model equation was obtained for each response.The optimum yield of the bio-oil and its HHV were obtained as 51.03%and 21.87 mJ/kg,respectively,at 545oC,45.17oC/min and 2.504 g of pyrolysis temperature,heating rate and catalyst weight,respectively.The high R2 values of 0.9859 and 0.9527,respectively,obtained for the bio-oil yield and its HHV models using analysis of variance revealed that the models can adequately predict the bio-oil yield and its HHV from the pyrolysis process.展开更多
Objective This study is to explore the relationship between acetylcholine(ACh)-induced calcium release from intracellular Ca2+ stores and function of outer hair cell(OHC) motors, in an attempt to elucidate the mechani...Objective This study is to explore the relationship between acetylcholine(ACh)-induced calcium release from intracellular Ca2+ stores and function of outer hair cell(OHC) motors, in an attempt to elucidate the mechanism of OHC electromotility at resting state. Methods OHCs were isolated from adult guinea pig (200-300 g) cochlea and loaded with Fluo-3/AM. The cells were treated with ACh/dHBSS, ACh/HBSS, dHBSS only or HBSS only. Intracellular [Ca2+]i variations in cells under the four treatments were observed using an Ar-Kr laser scan confocal microscope. Results [Ca2+]i oscillations were recorded in five OHCs treated with ACh/dHBSS but not in other cells. This is the first time that Ach-excited [Ca2+]i oscillations are reported in guinea pig OHCs independent of extracellular calcium. Conclusions ACh-excited [Ca2+]i oscillations in OHCs originates from intracellular calcium release and may play a crucial role in maintaining active mechanical motility of the OHC at resting and modulating OHC electromotility.展开更多
The damage to spiral organs related to age and sex in guinea pigs was studied by the surface preparation technique. Hair cell loss of spiral organs of the following four age groups: 8month, 1, 2, and 2.5-year old pig...The damage to spiral organs related to age and sex in guinea pigs was studied by the surface preparation technique. Hair cell loss of spiral organs of the following four age groups: 8month, 1, 2, and 2.5-year old pigs, was assessed. Each group was divided Into two subgroups according to different sexes. The decrease of hair cell population was linearly related to age, but not related to sex. The damage rate was greater in outer hair cells than in inner hair calls. In all of animals of different ages and both sexes, outer hair cell loss was prominent at the apex of cochlea. Less damage to outer hair cell was found at the base of cochlea. Damage to inner hair cell was also increased with age, but to a lesser extent.展开更多
Objective Adipose-derived stem cells(ADSCs) are suggested to possess a highly plastic ability to differentiate into several specific cell types in addition to adipocyte lineages, including germ layer tissue-specific c...Objective Adipose-derived stem cells(ADSCs) are suggested to possess a highly plastic ability to differentiate into several specific cell types in addition to adipocyte lineages, including germ layer tissue-specific cell lineages such as chondrocyte, myocyte, neuronal, and osteoblast lineages. The aim of this study is to establish an in vitro culture technique for ADSCs in an adult guinea pig model that facilitate their differentiation into hair cell-like cells. Materials and Methods Cells from inguinal fat pads in adult guinea pigs were cultured with β-mercaptoethanol, RA, Forskorin, Heregulin, bFGF, BDNF and EGF. Cellular differentiation was examined using immunocytochemistry techniques. Results The ADSCs demonstrated hair cell immunophenotypes with expression of epitopes of the hair cell marker protein myosin Ⅶa. Conclusion ADSCs from adult guinea pig adipose tissue can differentiate into hair cell-like cells when cultured in vitro. ADSCs may serve as seed cells for tissue engineering.展开更多
The waltzing guinea pig may be a good model to investigate if genetic factor can change the sensitivity in noise-induced hearing loss. A total of 34 waltzig guinea pigs were studied and we found that there is no any s...The waltzing guinea pig may be a good model to investigate if genetic factor can change the sensitivity in noise-induced hearing loss. A total of 34 waltzig guinea pigs were studied and we found that there is no any significant increased sensitivity to noise trauma if the age-induced hearing loss was considered in waltz-ing guinea pig.展开更多
文摘The low yield and poor fuel properties of bio-oil have made the pyrolysis production process uneconomic and also limited bio-oil usage.Proper manipulation of key pyrolysis variables is paramount in order to produce high-quality bio-oil that requires less upgrading.In this research,the pyrolysis of pig hair was carried out in a fixed-bed reactor using a calcium oxide catalyst derived from calcination of turtle shells.In the pyrolysis process,the influence of three variables-temperature,heating rate and catalyst weight-on two responses-bio-oil yield and its higher heating value(HHV)-were investigated using Response Surface Methodology.A second-order regression-model equation was obtained for each response.The optimum yield of the bio-oil and its HHV were obtained as 51.03%and 21.87 mJ/kg,respectively,at 545oC,45.17oC/min and 2.504 g of pyrolysis temperature,heating rate and catalyst weight,respectively.The high R2 values of 0.9859 and 0.9527,respectively,obtained for the bio-oil yield and its HHV models using analysis of variance revealed that the models can adequately predict the bio-oil yield and its HHV from the pyrolysis process.
文摘Objective This study is to explore the relationship between acetylcholine(ACh)-induced calcium release from intracellular Ca2+ stores and function of outer hair cell(OHC) motors, in an attempt to elucidate the mechanism of OHC electromotility at resting state. Methods OHCs were isolated from adult guinea pig (200-300 g) cochlea and loaded with Fluo-3/AM. The cells were treated with ACh/dHBSS, ACh/HBSS, dHBSS only or HBSS only. Intracellular [Ca2+]i variations in cells under the four treatments were observed using an Ar-Kr laser scan confocal microscope. Results [Ca2+]i oscillations were recorded in five OHCs treated with ACh/dHBSS but not in other cells. This is the first time that Ach-excited [Ca2+]i oscillations are reported in guinea pig OHCs independent of extracellular calcium. Conclusions ACh-excited [Ca2+]i oscillations in OHCs originates from intracellular calcium release and may play a crucial role in maintaining active mechanical motility of the OHC at resting and modulating OHC electromotility.
文摘The damage to spiral organs related to age and sex in guinea pigs was studied by the surface preparation technique. Hair cell loss of spiral organs of the following four age groups: 8month, 1, 2, and 2.5-year old pigs, was assessed. Each group was divided Into two subgroups according to different sexes. The decrease of hair cell population was linearly related to age, but not related to sex. The damage rate was greater in outer hair cells than in inner hair calls. In all of animals of different ages and both sexes, outer hair cell loss was prominent at the apex of cochlea. Less damage to outer hair cell was found at the base of cochlea. Damage to inner hair cell was also increased with age, but to a lesser extent.
文摘Objective Adipose-derived stem cells(ADSCs) are suggested to possess a highly plastic ability to differentiate into several specific cell types in addition to adipocyte lineages, including germ layer tissue-specific cell lineages such as chondrocyte, myocyte, neuronal, and osteoblast lineages. The aim of this study is to establish an in vitro culture technique for ADSCs in an adult guinea pig model that facilitate their differentiation into hair cell-like cells. Materials and Methods Cells from inguinal fat pads in adult guinea pigs were cultured with β-mercaptoethanol, RA, Forskorin, Heregulin, bFGF, BDNF and EGF. Cellular differentiation was examined using immunocytochemistry techniques. Results The ADSCs demonstrated hair cell immunophenotypes with expression of epitopes of the hair cell marker protein myosin Ⅶa. Conclusion ADSCs from adult guinea pig adipose tissue can differentiate into hair cell-like cells when cultured in vitro. ADSCs may serve as seed cells for tissue engineering.
基金supported by Tysta Skolan and Karolinska Institutet,Stockholm,Sweden
文摘The waltzing guinea pig may be a good model to investigate if genetic factor can change the sensitivity in noise-induced hearing loss. A total of 34 waltzig guinea pigs were studied and we found that there is no any significant increased sensitivity to noise trauma if the age-induced hearing loss was considered in waltz-ing guinea pig.
