Background： The stomach is an underestimated key interface between the ingesta and the digestive system, affecting the digestion and playing an important role in several endocrine functions. The quality of starter mi...Background： The stomach is an underestimated key interface between the ingesta and the digestive system, affecting the digestion and playing an important role in several endocrine functions. The quality of starter microbiota and the early life feeding of medium chain triglycerides may affect porcine gastric maturation. Two trials（T1, T2） were carried out on 12 and 24 cesarean-delivered piglets（birth, d0）, divided over two microbiota treatments, but slaughtered and sampled at two or three weeks of age, respectively. All piglets were fed orally： sow serum（T1） or pasteurized sow colostrum（T2） on d0; simple starter microbiota（Lactobacillus amylovorus, Clostridium glycolicum and Parabacteroides spp.）（d1-d3）; complex microbiota inoculum（sow diluted feces, CA） or a placebo（simple association, SA）（d3-d4） and milk replacer ad libitum（d0-d4）. The The T1 piglets and half of the T2 piglets were then fed a moist diet（CTRL）; the remaining half of the T2 piglets were fed the CTRL diet fortified with medium chain triglycerides and 7% coconut oil（MCT）. Total m RNA from the oxyntic mucosa was analyzed using Affymetrix？Porcine Gene array strips. Exploratory functional analysis of the resulting values was carried out using Gene Set Enrichment Analysis.Results： Complex microbiota upregulated 11 gene sets in piglets of each age group vs. SA. Of these sets, 6 were upregulated at both ages, including the set of gene markers of oxyntic mucosa. In comparison with the piglets receiving SA, the CA enriched the genes in the sets related to interferon response when the CTRL diet was given while the same sets were impoverished by CA with the MCT diet.Conclusions： Early colonization with a complex starter microbiota promoted the functional maturation of the oxyntic mucosa in an age-dependent manner. The dietary fatty acid source may have affected the recruitment and the maturation of the immune cells, particularly when the piglets were early associated with a simplified starter microbiota.展开更多
目的:明确黄连提取物及其化学成分对豚鼠胃平滑肌收缩运动的影响,探讨与黄连"健胃"和"败胃"作用相关的药效物质基础。方法:采用张力测定法,观察黄连水提物、黄连总生物碱(0.3～1 000μg/mL)及其化学成分小檗碱、巴...目的:明确黄连提取物及其化学成分对豚鼠胃平滑肌收缩运动的影响,探讨与黄连"健胃"和"败胃"作用相关的药效物质基础。方法:采用张力测定法,观察黄连水提物、黄连总生物碱(0.3～1 000μg/mL)及其化学成分小檗碱、巴马丁碱和药根碱(0.3～1000μmol/L)对豚鼠离体胃窦环行肌自发收缩和电场刺激(electrical field stimulation,EFS)诱发收缩的影响。结果:小剂量黄连水提物和黄连总生物碱均能促进胃窦环行肌的自发收缩,而大剂量则抑制。小檗碱、巴马丁碱和药根碱具有相似效应,其中在发挥促收缩作用时药根碱作用最强,在发挥抑制作用时小檗碱作用最强。另外,黄连水提物、黄连总生物碱、小檗碱、巴马丁碱和药根碱均能增加EFS诱发的平滑肌收缩幅度,其中药根碱的作用最强。结论:小剂量黄连"健胃"与大剂量"败胃"作用可能与其影响胃平滑肌收缩有关。小檗碱、巴马丁碱和药根碱都是黄连影响胃平滑肌收缩的有效成分,药根碱促平滑肌收缩的作用最强,而小檗碱抑制平滑肌收缩的作用最强。展开更多
AIM:To evaluate the effect of hands-on training of gastroenterology fellows in gastric polypectomy using an ex vivo simulator.METHODS:Eight gastroenterology fellows at Mackay Memorial Hospital,Taipei were evaluated in...AIM:To evaluate the effect of hands-on training of gastroenterology fellows in gastric polypectomy using an ex vivo simulator.METHODS:Eight gastroenterology fellows at Mackay Memorial Hospital,Taipei were evaluated in gastricpolypectomy techniques using a pig stomach with artificial polyps created by a rubber band ligation device.The performance of four second year(year-2)fellows who had undergone one year of clinical training was compared with that of four f irst year(year-1)fellows both before and after a 4-h workshop using the ex vivo simulator.The workshop allowed for hands-on train-ing in the removal of multiple artif icial polyps and the placement of hemoclips at the excision site.Evaluation included observation of technical skills,procedure time,and the fellows' conf idence scale.RESULTS:One week after the workshop,the year-1 fellows were re-evaluated and had significantly im-proved mean performance scores(from 17.9 ± 1.8 to 22.5 ± 0.7),conf idence scale(from 4.5 ± 1.0 to 7.8 ± 0.5)and procedure time(from 615.0 ± 57.4 s to 357.5 ± 85.0 s)compared with their baseline performance.After 4 h of training using the ex vivo simulator,the skills of the year-1 fellows were statistically similar to those of the year-2 fellows.CONCLUSION:Use of this ex vivo simulator significantly improved the endoscopic gastric polypectomy skills of gastroenterology fellows who had not had previous clinical training in gastric polypectomy.展开更多
基金financially supported by the European Union(contract No.227549)through the Interplay project
文摘Background： The stomach is an underestimated key interface between the ingesta and the digestive system, affecting the digestion and playing an important role in several endocrine functions. The quality of starter microbiota and the early life feeding of medium chain triglycerides may affect porcine gastric maturation. Two trials（T1, T2） were carried out on 12 and 24 cesarean-delivered piglets（birth, d0）, divided over two microbiota treatments, but slaughtered and sampled at two or three weeks of age, respectively. All piglets were fed orally： sow serum（T1） or pasteurized sow colostrum（T2） on d0; simple starter microbiota（Lactobacillus amylovorus, Clostridium glycolicum and Parabacteroides spp.）（d1-d3）; complex microbiota inoculum（sow diluted feces, CA） or a placebo（simple association, SA）（d3-d4） and milk replacer ad libitum（d0-d4）. The The T1 piglets and half of the T2 piglets were then fed a moist diet（CTRL）; the remaining half of the T2 piglets were fed the CTRL diet fortified with medium chain triglycerides and 7% coconut oil（MCT）. Total m RNA from the oxyntic mucosa was analyzed using Affymetrix？Porcine Gene array strips. Exploratory functional analysis of the resulting values was carried out using Gene Set Enrichment Analysis.Results： Complex microbiota upregulated 11 gene sets in piglets of each age group vs. SA. Of these sets, 6 were upregulated at both ages, including the set of gene markers of oxyntic mucosa. In comparison with the piglets receiving SA, the CA enriched the genes in the sets related to interferon response when the CTRL diet was given while the same sets were impoverished by CA with the MCT diet.Conclusions： Early colonization with a complex starter microbiota promoted the functional maturation of the oxyntic mucosa in an age-dependent manner. The dietary fatty acid source may have affected the recruitment and the maturation of the immune cells, particularly when the piglets were early associated with a simplified starter microbiota.
文摘目的:明确黄连提取物及其化学成分对豚鼠胃平滑肌收缩运动的影响,探讨与黄连"健胃"和"败胃"作用相关的药效物质基础。方法:采用张力测定法,观察黄连水提物、黄连总生物碱(0.3～1 000μg/mL)及其化学成分小檗碱、巴马丁碱和药根碱(0.3～1000μmol/L)对豚鼠离体胃窦环行肌自发收缩和电场刺激(electrical field stimulation,EFS)诱发收缩的影响。结果:小剂量黄连水提物和黄连总生物碱均能促进胃窦环行肌的自发收缩,而大剂量则抑制。小檗碱、巴马丁碱和药根碱具有相似效应,其中在发挥促收缩作用时药根碱作用最强,在发挥抑制作用时小檗碱作用最强。另外,黄连水提物、黄连总生物碱、小檗碱、巴马丁碱和药根碱均能增加EFS诱发的平滑肌收缩幅度,其中药根碱的作用最强。结论:小剂量黄连"健胃"与大剂量"败胃"作用可能与其影响胃平滑肌收缩有关。小檗碱、巴马丁碱和药根碱都是黄连影响胃平滑肌收缩的有效成分,药根碱促平滑肌收缩的作用最强,而小檗碱抑制平滑肌收缩的作用最强。
文摘AIM:To evaluate the effect of hands-on training of gastroenterology fellows in gastric polypectomy using an ex vivo simulator.METHODS:Eight gastroenterology fellows at Mackay Memorial Hospital,Taipei were evaluated in gastricpolypectomy techniques using a pig stomach with artificial polyps created by a rubber band ligation device.The performance of four second year(year-2)fellows who had undergone one year of clinical training was compared with that of four f irst year(year-1)fellows both before and after a 4-h workshop using the ex vivo simulator.The workshop allowed for hands-on train-ing in the removal of multiple artif icial polyps and the placement of hemoclips at the excision site.Evaluation included observation of technical skills,procedure time,and the fellows' conf idence scale.RESULTS:One week after the workshop,the year-1 fellows were re-evaluated and had significantly im-proved mean performance scores(from 17.9 ± 1.8 to 22.5 ± 0.7),conf idence scale(from 4.5 ± 1.0 to 7.8 ± 0.5)and procedure time(from 615.0 ± 57.4 s to 357.5 ± 85.0 s)compared with their baseline performance.After 4 h of training using the ex vivo simulator,the skills of the year-1 fellows were statistically similar to those of the year-2 fellows.CONCLUSION:Use of this ex vivo simulator significantly improved the endoscopic gastric polypectomy skills of gastroenterology fellows who had not had previous clinical training in gastric polypectomy.
