Grain Yield,Biomass Accumulation,and Leaf Photosynthetic Characteristics of Rice under Combined Salinity-Drought Stress

Simultaneous stresses of salinity and drought often coincide during rice-growing seasons in saline lands,primarily due to insufficient water resources and inadequate irrigation facilities.Consequently,combined salinity-drought stress poses a major threat to rice production.In this study,two salinity levels(NS,non-salinity;HS,high salinity)along with three drought treatments(CC,control condition;DJ,drought stress imposed at jointing;DH,drought stress imposed at heading)were performed to investigate their combined influences on leaf photosynthetic characteristics,biomass accumulation,and rice yield formation.Salinity,drought,and their combination led to a shortened growth period from heading to maturity,resulting in a reduced overall growth duration.Grain yield was reduced under both salinity and drought stress,with a more substantial reduction under the combined salinity-drought stress.The combined stress imposed at heading caused greater yield losses in rice compared with the stress imposed at jointing.Additionally,the combined salinity-drought stress induced greater decreases in shoot biomass accumulation from heading to maturity,as well as in shoot biomass and nonstructural carbohydrate(NSC)content in the stem at heading and maturity.However,it increased the harvest index and NSC remobilization reserve.Salinity and drought reduced the leaf area index and SPAD value of flag leaves and weakened the leaf photosynthetic characteristics as indicated by lower photosynthetic rates,transpiration rates,and stomatal conductance.These reductions were more pronounced under the combined stress.Salinity,drought,and especially their combination,decreased the activities of ascorbate peroxidase,catalase,and superoxide dismutase,while increasing the contents of malondialdehyde,hydrogen peroxide,and superoxide radical.Our results indicated a more significant yield loss in rice when subjected to combined salinity-drought stress.The individual and combined stresses of salinity and drought diminished antioxidant enzyme activities,inhibited leaf photosynthetic functions,accelerated leaf senescence,and subsequently lowered assimilate accumulation and grain yield.

Implications for identification of principal stress directions from acoustic emission characteristics of granite under biaxial compression experiments

The rock fracture characteristics and principal stress directions are crucial for prevention of geological disasters.In this study,we carried out biaxial compression tests on cubic granite samples of 100 mm in side length with different intermediate principal stress gradients in combination with acoustic emission(AE)technique.Results show that the fracture characteristics of granite samples change from‘sudden and aggregated’to‘continuous and dispersed’with the increase of the intermediate principal stress.The effect of increasing intermediate principal stress on AE amplitude is not significant,but it increases the proportions of high-frequency AE signals and shear cracks,which in turn increases the possibility of unstable rock failure.The difference of stress in different directions causes the anisotropy of rock fracture and thus leads to the obvious anisotropic characteristics of wave velocity variations.The anisotropy of wave velocity variations with stress difference is probable to identify the principal stress directions.The AE characteristics and the anisotropy of wave velocity variations of granite under two-dimensional stress are not only beneficial complements for rock fracture characteristic and principal stress direction identification,but also can provide a new analysis method for stability monitoring in practical rock engineering.

Load-bearing characteristics and energy evolution of fractured rock masses after granite and sandstone grouting

Experiments on grouting-reinforced rock mass specimens with different particle sizes and features were carried out in this study to examine the effects of grouting reinforcement on the load-bearing characteristics of fractured rock mass.The strength and deformation features of grouting-reinforced rock mass were analyzed under different loading manners;the energy evolution mechanism of grouting-reinforced rock mass specimens with different particle sizes and features was investigated;the energy dissipation ratio and post-peak stress decreasing rate were employed to evaluate the bearing stability of grouting-reinforced rock mass.The results show that the strength and ductility of granite-reinforced rock mass(GRM)under biaxial loading are higher than that of sandstone-reinforced rock mass(SRM)under uniaxial loading.Besides,the energy evolution characteristics of grouting-reinforced rock mass under uniaxial and biaxial loading mainly could be divided into early,middle,and late stages.In the early stage,total,elastic,and dissipation energies were quite small with flatter curves;in the middle stage,elastic energy increased rapidly,whereas dissipation energy increased slowly;in the late stage,dissipation energy increased sharply.The energy dissipation ratio was used to represent the pre-peak plastic deformation.Under uniaxial loading,this ratio increased as the particle size increased and the pre-peak plastic deformation of grouting-reinforced rock mass became larger;under biaxial loading,it dropped as the particle size increased,and the pre-peak plastic deformation of grouting-reinforced rock mass became smaller.The post-peak stress decline rate A_(v) was used to assess the post-peak bearing performance of grouting-reinforced rock mass.Under uniaxial loading,parameter A_(v) exhibited reduction as the particle size kept increasing,and the ability of post-peak of grouting-reinforced rock mass to allow deformation development was greater,and the bearing capacity was greater;under biaxial loading,A_(v) increased with the particle size,and the ability of post-peak of grouting-reinforced rock mass to allow deformation development was low and the bearing capacity was reduced.The findings are considered instrumental in improving the stability of the roadway-surrounding rock by granite and sandstone grouting.

Study on Sealing Characteristics of Sliding Seal Assembly of Aircraft Hydraulic Actuator

The hydraulic actuator,known as the"muscle"of military aircraft,is responsible for flight attitude adjustment,trajectory control,braking turn,landing gear retracting and other actions,which directly affect its flight efficiency and safety.However,the sealing assembly often has the situation of over-aberrant aperture fit clearance or critical over-aberrant clearance,which increases the failure probability and degree of movable seal failure,and directly affects the flight efficiency and safety of military aircraft.In this paper,the simulation model of hydraulic actuator seal combination is established by ANSYS software,and the sealing principle is described.The change curve of contact width and contact pressure of combination seal under the action of high-pressure fluid is drawn.The effects of different oil pressure,fit clearance and other parameters on the sealing performance are analyzed.Finally,the accelerated life test of sliding seal components is carried out on the hydraulic actuator accelerated life test rig,and the surface morphology is compared and analyzed.The research shows that the O-ring is the main sealing element and the role of the check ring is to protect and support the O-ring to prevent damage caused by squeezing into the fit clearance,so the check ring bears a large load and is prone to shear failure.Excessive fit clearance is the main factor affecting the damage of the check ring,and the damage parts are mainly concentrated at the edge of the sealing surface.This paper provides a theoretical basis for the design of hydraulic actuator and the improvement of sealing performance.

Stress response characteristics of BFRP anchors on loess mudstone slope under rainfall conditions

To investigate the stress response characteristics and shear stress transfer mechanism of BFRP(basalt fiber reinforced plastics)anchors under rainfall conditions and to explore the reinforcement effect of BFRP anchors,a comparative indoor physical model test was conducted in this study using loess mudstone slope as a typical case,and multi-attribute response data,such as slope displacement,BFRP anchor strain and axial force,were obtained.Based on the variation law of slope displacement,it can be concluded that the deformation displacement of the slope on the side reinforced by BFRP anchors is smaller than that of slopes reinforced with steel anchors;the bearing capacity of BFRP anchor is given priority in time,and the anchoring effect of BFRP anchor at the top of the slope is given priority,and the axial force value of anchor is characterized by a surface amplification effect in space;the axial force is higher around the anchor head and tends to decay as a negative exponential function as the anchor extends in the direction of the interior of the slope;the shear stress of BFRP anchor and anchorage body are unevenly distributed along the axial direction,with the maximum value occurring in the free section near the slope surface and decreases as the BFRP anchor extends towards the interior of the slope.These results can provide a theoretical basis for the optimal design of BFRP anchors.

Water infiltration and soil-water characteristics of compacted loess under applied vertical stress

Additional stress formed by postconstruction buildings in loess-filling areas affects water infiltration in soil and causes soil deformation.To investigate this effect,under constant water head,vertical infiltration tests on compacted loess with two initial dry densities for different applied vertical stresses were developed using vertical stresscontrollable one-dimensional soil columns.The timehistory curves of vertical deformation,wetting front depth,cumulative infiltration depth,volumetric water content(VWC)and suction were measured,and the soil-water characteristic curves(SWCCs)were determined.The results showed that:(1)the infiltration ability of the soil column weakens with increasing applied vertical stress and initial dry density;(2)vertical deformation increases rapidly at first and then tends to be stable slowly at the consolidation and wetting-induced deformation stage,and is positively correlated with applied vertical stress and is negatively correlated with initial dry density.The stability time of wetting-induced deformation and the corresponding wetting front depth increase with the increase of applied vertical stress,while they decrease obviously when initial dry density increases;(3)the influence of applied vertical stress on soilwater characteristics in soil columns with various initial dry densities is related to the deformation depth of soil column.The VG(Van Genuchten)model is suitable for fitting the SWCCs at different monitoring positions.A normalized SWCC model introducing the applied vertical stress was proposed for each initial dry density using the mathematical relationship between the fitting parameters and the applied vertical stress.

Osmotic Regulation,Antioxidant Enzyme Activities and Photosynthetic Characteristics of Tree Peony(Paeonia suffruticosa Andr.)in Response to High-Temperature Stress

Tree peony(Paeonia suffruticosa Andr.)is a traditional Chinese flower,which prefers cool weather.However,high temperature in summer in the middle and lower reaches of the Yangtze River restricts its growth and development.In this study,osmotic regulation,antioxidant enzyme activities,and photosynthetic characteristics of tree peony in response to high-temperature stress were investigated.The results showed that high-temperature stress had destroyed the cell membrane,manifested as the increased relative electrical conductivity and malondialdehyde content.Moreover,high-temperature stress led to excessive accumulation of reactive oxygen species,thereby,activating antioxidant enzyme activities.Also,photosynthetic parameters and chlorophyll fluorescence parameters directly reflected the damage to the photosystem II reflection center under high-temperature stress.In addition,high-temperature stress led to stomatal closure and chloroplast damage.This study revealed the physiological responses of tree peony to high-temperature stress,laying a foundation for the promotion of tree peony in high-temperature areas and the improvement of high-temperature resistance.

Expression Characteristics of AaHsp90 Gene in Antheraea assamensis under Different Temperature and Starvation Stress

[Objectives]To investigate the effects of different temperature and starvation stress on the expression of AaHsp90 and reveal the molecular mechanism of adaptation to environment in Antheraea assama.[Methods]Taking the normal feeding group at 26℃as the control,the expression change of AaHsp90 was detected by real-time PCR in midgut,fat body and hemlymph after high temperature stress at 38℃,low temperature stress at 4℃and starvation stress separately for different time on the third day of the fifth larvae.[Results]The expression of AaHsp90 in midgut,fat body and hemlymph of Antheraea assama were increased obviously at first and then decreased sharply with the prolongation of treatment time at 38℃.There has a certain inhibitory effect on the expression of AaHsp90 in midgut,fat body and hemolymph after treatment with 4℃for different time.After treatment with starvation,the AaHsp90 expression were increased at 12 and 18 h and decreased sharply at 24 h in midgut,fat body and hemolymph of A.assama.[Conclusions]Comprehensive analysis showed that high temperature and starvation stress can induce the expression of AaHsp90,while low temperature stress mainly suppressed its expression.It was suggested that the AaHsp90 protein may play an important role in the process of adaptation to high temperature and starvation stress in A.assama.

Effects of Heat Stress during Seed Filling Stage on Brassica napus Seed Oil Accumulation and Chlorophyll Fluorescence Characteristics

As global temperature rise,the threat of heat stress to rapeseed production is becoming more obvious.Exploring the response characteristics of two important biological pathways,oil accumulation and photosynthesis,to heat stress during B.napus seed filling is helpful in the genetic improvement of heat-tolerant rapeseed.The effects of heat stress on seed oil accumulation and chlorophyll fluorescence characteristics of 29 B.napus germplasms with different oil content and environmental sensitivity,including 6 rapeseed varieties which exhibited environmentsensitive/insensitive and with high,medium or low oil content,were tested by whole plant heat stress or the in vitro silique culture system.Both assay exhibited similar trend on oil content of the rapeseed germplasms.The heat effect on the chlorophyll fluorescence kinetic parameters F_(v)/F_(m),ETR and Y(Ⅱ)were also consistent.Heat stress significantly decreased oil content,although there was abundant genetic variation on heat tolerance among the genotypes.Correlation analysis showed that the decrease rate of F_(v)/F_(m) of silique heat-stressed B.napus developing seed was positive correlative to the decrease rate of mature seed oil content of the whole plant heat-stressed rapeseed(R=0.9214,P-value<0.01).Overall,the results indicated that heat stress inhibited oil accumulation and photosynthesis in B.napus developing seed.The decrease rate of chlorophyll fluorescence parameter F_(v)/F_(m) of heat-stressed developing seed could be used as the index of heat tolerant rapeseed identification.Further,two heat insensitive rapeseed varieties with high oil content were identified.

Effects of Water Stress on Photosynthesis and Fluorescence Characteristics in Peony

[ Objective] Study on the photosynthesis and fluorescence characteristics in peony under water stress. [ Method ] The two peony varieties Huhong and Luoyanghong were treated by different water stress for determining the photosynthesis and fluorescence characteristics. [ Result ] With the aggravation of water stress, the net photosynthetic rate （Pn） and stomatal conductance （Gs） decreased, while the intercellular CO2 concentration （Ci） increased. Drought could decrease Pn, constrain gas exchange and change the daily photosynthesis. Fo of peony leaf increased and Fv/Fm decreased under water stress especially water logging, causing the inactivation of the PS II reaction center, and the chlorophyll fluorescence characters gradually recovered until afternoon. [ Conclusion] The 75% soil relative water content （SRWC） is the best condition for growth of peony. Compared with drought, water logging is more unfit for the growth of peony. For the two varieties, Huhong assumed more tolerance to drought, accordingly more adaptability.

Effects of Antimony Stress on Photosynthetic Characteristics of Vegetable Leaves

[Objective] This study aimed to investigate the effects of different concen- trations of antimony and modifier calcium magnesium phosphate on photosynthetic characteristics of edible amaranth, flowering Chinese cabbage, spinach and flowering Chinese cabbage. [Method] By outdoor potting simulation experiment, soil matrixes containing 10.00, 20.00, 50.00, 70.00 and 100.00 mg/kg antimony （Sb3＋） were pre- pared; soil without antimony was used as control （CK）. Each pot was loaded with 0.10 kg/kg vegetable special fertilizer, mixed evenly, and divided into two shares： one share was supplemented with 1.75 g/kg modifier calcium magnesium phosphate and mixed evenly; the other share contained no calcium magnesium phosphate. Af- ter the generation of three true leaves, seedlings with uniform growth were trans- planted into the prepared soil matrixes, eights seedlings per pot. Vegetable seedlings were watered regularly to maintain 70% of field capacity. After 45 d, veg- etable plants were harvested and washed clean with distilled water for measurement of indicators of photosynthetic characteristics. [Result] With the increase of antimony concentration, relative chlorophyll content （SPAD value） and net photosynthetic rate of four vegetable species increased first and then declined, while stomatal conduc- tance of vegetable leaves was linearly reduced. [Conclusion] Appropriately adding modifier calcium magnesium phosphate can effectively improve the photosynthetic characteristics of four vegetable species and reduce the toxic effects of heavy metal antimony on vegetables.

Preliminary Study on the Accumulation Characteristics of Mineral Elements in Potted Poa crymophila under Drought and Water Stress

[Objective] This study was conducted to explain the mechanism of the accumulation characteristics of mineral elements in alpine grassland plants. [Method] Cultivated alpine grassland plant, Poa crymophila, was treated with drought and wa- ter stress, and then the samples were collected and analyzed. [Result] Compared with the control group, under drought and water stress, multiple mineral elements tended to accumulate and increase, and there were significant differences in the contents of Cu, Mn, Ni and P （P〈0.05）. [Conclusion] Under drought and water stress, mineral elements in potted Poa crymophila tended to accumulate and in- crease, which is the adaption and response of Poa crymophila to drought and water stress, as well as the re-verification of the starvation effect hypothesis of mineral effects. The starvation effect of mineral elements is one of the endogenic forces for the accumulation and differentiation of mineral elements in grassland plants.

Effect of Lead Stress on Growth Characteristics and Physiological Indexes of Alternanthera philoxeroides

[Objective] The paper aimed to discuss effects of different concentrations of lead stress on Alternanthera philoxeroides growth and physiological indices,to explore phytoremediation methods for removal of lead pollution. [Method] A. philoxeroides in water culture was treated with different concentration of lead,and then,its external injury was observed,its growth and physiological indexes were measured. [Result] A. philoxeroides biomass and chlorophyll content decreased; conductivity and malonaldehyde （MDA ） content were increased; the SOD,POD and CAT activities showed a trend of first increase and then decrease with increase in concentration of lead stress. [Conclusion] With increase in lead concentration,the cell membrane permeability increased and MDA had a higher accumulation,antioxidant enzymes activity decreased,the normal oxidative metabolism of A. philoxeroides was severely affected,leading it to wilt.

Salt Stress Effect on the Growth and Physiological Characteristics of Three New Varieties of Lagerstroemia indica

The effects of different concentrations of NaCI （0, 0.10%, 0.25%, 0.40%, 0.55%, 0.70%） on the growth and physiological characteristics of three new varieties of Lagerstroemia indica were studied by pot experiment in the plastic greenhouses. The results showed that under different NaCI concentrations, the relative height in- crement and the relative diameter increment of L. indica were restrained obviously; the contents of chlorophyll a, chlorophyll b, carotenoid and GSH decreased with in- crease of NaCI concentration; and the content of MDA increased with increase of NaCI concentration. Through the determination and comparison of physiological in- dexes, and analysis of correlativity, NaCI tolerance of the three new L. indica vari- eties ranked as L. indica ＇Pink Velour＇〉L. indica ＇Dynamite＇〉L. indica ＇Red Rock- et＇. This is consistent with the results of the morphological characteristics after Na- CI stress.

Rapid testing and prediction of soil–water characteristic curve of subgrade soils considering stress state and degree of compaction

The subgrade soil is generally in saturated or unsaturated condition. To analyze complex thermo-hydro-mechanical-chemical (THMC) behaviors of subgrade, it is essential to determine the soil–water characteristic curve (SWCC) that represents the relationship between matric suction and moisture content. In this study, a full-automatic rapid stress-dependent SWCC pressure-plate extractor was developed. Then, the influences of overburden stress and degree of compaction on the SWCC of subgrade soil such as high liquid limit silt (MH) and low liquid limit clay (CL) were analyzed. Accordingly, a new model taking into account the influences of overburden stress and degree of compaction based on the well-known Van Genuchten (VG) SWCC fitting model was presented and validated. The results show that with the increase of the degree of compaction and overburden stress, the saturated moisture content of subgrade soil decreases, while the air-entry value increases and the transition section curve becomes flat. The influences of the degree of compaction and overburden stress on the SWCC of MH is greater than that of CL. Meanwhile, there was a satisfactory agreement between the prediction and measurement, indicating a good performance of the new model for predicting the SWCC.

Impact of effective stress on permeability for carbonate fractured-vuggy rocks

To gain insight into the flow mechanisms and stress sensitivity for fractured-vuggy reservoirs,several core models with different structural characteristics were designed and fabricated to investigate the impact of effective stress on permeability for carbonate fractured-vuggy rocks(CFVR).It shows that the permeability performance curves under different pore and confining pressures(i.e.altered stress conditions)for the fractured core models and the vuggy core models have similar change patterns.The ranges of permeability variation are significantly wider at high pore pressures,indicating that permeability reduction is the most significant during the early stage of development for fractured-vuggy reservoirs.Since each obtained effective stress coefficient for permeability(ESCP)varies with the changes in confining pressure and pore pressure,the effective stresses for permeability of four representative CFVR show obvious nonlinear characteristics,and the variation ranges of ESCP are all between 0 and 1.Meanwhile,a comprehensive ESCP mathematical model considering triple media,including matrix pores,fractures,and dissolved vugs,was proposed.It is proved theoretically that the ESCP of CFVR generally varies between 0 and 1.Additionally,the regression results showed that the power model ranked highest among the four empirical models mainly applied in stress sensitivity characterization,followed by the logarithmic model,exponential model,and binomial model.The concept of“permeability decline rate”was introduced to better evaluate the stress sensitivity performance for CFVR,in which the one-fracture rock is the strongest,followed by the fracture-vug rock and two-horizontalfracture rock;the through-hole rock is the weakest.In general,this study provides a theoretical basis to guide the design of development and adjustment programs for carbonate fractured-vuggy reservoirs.

Effects of High Temperature Stress on Microscopic and Ultrastructural Characteristics of Mesophyll Cells in Flag Leaves of Rice

The microscopic and ultrastructural characteristics of mesophyll cells in flag leaves of two rice lines （a thermosensitive line 4628 and a thermo-resistant line 996） under high temperature stress （37℃ during 8：00-17：00 and 30℃ during 17：00-8：00） were investigated using an optical and a transmission electron microscopy. The membrane permeability and malondialdehyde content increased under the high temperature stress, and the increase of both variables was greater in the line 4628 than in the line 996. Under the high temperature stress, the line 996 showed tightly arranged mesophyll cells in flag leaves, fully developed vascular bundles and some closed stomata, whereas the line 4628 suffered from injury because of undeveloped vascular bundles, loosely arranged mesophyll cells and opened stomata. The mesophyll cells in flag leaves of the line 4628 were severely damaged under the high temperature stress, i.e. the chloroplast envelope became blurred, the grana thylakoid layer was arranged loosely and irregularly, the stroma layer disappeared, many osmiophilic granules appeared within the chloroplast, the outer membrane of mitochondria and the nucleus disintegrated and became blurred, the nucleolus disappeared, and much fibrillar-granular materials appeared within the nucleus. In contrast, the mesophyll cells in flag leaves of the line 996 maintained an intact ultrastructure under the high temperature stress. From these results, it is suggested that the ultrastructural modification of the cell membrane system is the primary plant response to high temperature stress and can be used as an index to evaluate the crop heat tolerance.

Response of leaf photosynthetic characteristics of Syringa oblata and Syringa reticulata var.mandshurica to chilling stress

Syringa species not only have good ornamental properties but also play an important role in the landscaping and environmental purification of cities.To investigate the chilling stress resistance of Syringa oblata Lindl.and Syringa reticulata var.mandshurica and provide theoretical grounds for the practical cultivation of Syringa species,in vitro leaves were used to study photosynthetic gas exchange parameters and chlorophyll fluorescence parameters.After nine hours of chilling,decreasing rates of net photosynthesis,stomatal conductance,and transpiration in S.reticulata var.mandshurica leaves were significantly greater than that of the S.oblata,while intercellular CO2 concentrations in S.oblata leaves were higher than those in S.reticulata var.mandshurica.The quantum yield of PSII reaction center(APSII)declined in S.reticulata and light capture efficiency(Fv 0/Fm 0)was stable.However,reduction percentages of Fv 0/Fm 0,APSII,and Fv/Fm in S.oblata were significant higher than those of S.reticulata var.mandshurica.After nine hours of chilling,the relative variable fluorescence of VJ and VI of S.oblata increased and the increasing rate of VJ was greater than VI.In contrast,the change of VJ and VI in S.reticulata var.mandshurica leaves was relatively small.This suggests that chilling primarily damaged the electron transport process of QA to QB at the receptor site of the PSII reaction center.Photosynthetic capacity of S.oblata was more sensitive to chilling stress compared to S.reticulate var.mandshurica,which the limitations were mainly due to non-stomatal factors such as the decrease in electron transport efficiency,activity in the PSII reaction center,and the destruction of the photodamage defense system.

Effect of non-uniform stress characteristics on stress measurement in specimen

There is a remarkable difference in stress distribution between a specimen and a plate removed from the specimen.The plate presents a uniform stress distribution whereas the specimen presents a non-uniform stress distribution.Firstly,the real stress distributions in plates with thickness of 30,40 and 50 mm and then in the specimens were obtained through simulation and X-ray surface stress measurement.Secondly,in order to study the impact of specimens shapes and processing ways on the results accuracy,two irregular shapes (parallelogram and trapezoid) and two processing ways (saw and electron discharge machining (EDM)) were compared and analyzed by simulation and experiment using layer removal method,then the specimen effects on measurement results were evaluated.The results show that:1) the non-uniform stress distribution characteristics of the specimen near the surface of the cut is significant,the range of non-uniform stress distribution is approximately one-thickness distance away from the cut,and it decreases gradually along the depth;2) In order to ensure the stability in the results,it is suitable to take the specimen plane size 2-3 times of its thickness;3) Conventional processing methods have little effect on experimental results and the average deviation is less than 5%.

Effects of Drought Stress Simulated by Polyethylene Glycol on Seed Germination, Root and Seedling Growth, and Seedling Antioxidant Characteristics in Job’s Tears

Two Job’s tears cultivars, yy18-1 (high resistance to drought stress) and yy12-7 (susceptible to drought stress) were used to investigate the responses of seed germination, root and seedling growth, and seedling antioxidant characteristics to drought stress simulated by polyethylene glycol (PEG) 6000 solutions with 0, -0.05, -0.1, -0.15, and -0.2 MPa osmotic potentials. The results showed that the germination energy, germination rate, germination index, root and seedling lengths, root and seedling diameters, root and seedling fresh masses, root and seedling dry masses, and seedling relative water content (RWC) decreased with the decrease of the osmotic potential of PEG 6000 solution. The contents of hydrogen peroxide (H2O2), malondialdehyde (MDA), and proline in seedling increased with the decrease of the osmotic potential of PEG 6000 solution. The activities of peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) in seedling increased and then decreased with the decrease of osmotic potential of PEG 6000 solution. -0.1 MPa was the optimal osmotic potential of PEG 6000 solution simulated drought stress at germination stage for Job’s tears. The proline content and activities of POD and CAT were important mechanisms for the maintenance of drought resistance in Job’s tears seedling.