[Objectives]To analyze the effect of Mongolian pharmaceutical Betel Shisanwei Ingredient Pills in the clinical treatment of patients with depression.[Methods]From June 2020 to May 2021,64 patients with depression who ...[Objectives]To analyze the effect of Mongolian pharmaceutical Betel Shisanwei Ingredient Pills in the clinical treatment of patients with depression.[Methods]From June 2020 to May 2021,64 patients with depression who received treatment in Inner Mongolia Minzu University were selected to participate in this study.These patients were randomly numbered from 1 to 64,and then divided into two groups according to the principle of odd or even number.Patients with odd number were regarded as the reference group,and treated by western medicine fluoxetine;patients with even number were regarded as the study group,and treated by Mongolian pharmaceutical Betel Shisanwei Ingredient Pills.The therapeutic effects of the two groups of patients were compared.[Results]Before treatment,there was no significant difference in the scores of patients'depressive syndromes between the two groups(P>0.05).After treatment,there were two significant changes in comprehensive score of depressive symptoms in both groups.Compared with before treatment,the data of the same group after treatment decreased significantly.Comparison between the two groups showed that the score of patients'depressive syndromes in the study group(13.28±5.49)was significantly lower than that in the reference group(18.46±6.51),and the overall response rate of treatment in the study group(96.88%,31/32)was obviously higher than that in the reference group(75.00%,24/32),showing statistically significant differences(P<0.05).[Conclusions]In the treatment of depression,Mongolian medicine therapy can significantly improve the depressive syndromes in patients,with more prominent curative effects,and is worthy of promotion and application.展开更多
目的:通过网络药理学和分子对接方法探讨麻子仁丸治疗功能性便秘的作用机制。方法:在TCMSP数据库中检索麻子仁丸中各药物的主要活性成分及作用靶点,并应用UniProt数据库筛选功能性便秘的相关疾病靶点。应用Cytoscape 3.7.2软件构建有效...目的:通过网络药理学和分子对接方法探讨麻子仁丸治疗功能性便秘的作用机制。方法:在TCMSP数据库中检索麻子仁丸中各药物的主要活性成分及作用靶点,并应用UniProt数据库筛选功能性便秘的相关疾病靶点。应用Cytoscape 3.7.2软件构建有效活性成分-靶点网络;用STRING数据库构建PPI网络,基于Bioconductor数据库利用R 3.6.3软件进行基因本体论(gene ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析,并提取前20条结果生成可视化图形。结果:获取麻子仁丸的主要活性成分57个;作用靶点167个,功能性便秘相关基因4948个,交集基因135个。较重要的有效活性成分包括木犀草素、山柰酚、柚皮素等。构建可视化PPI网络图共得到135个节点、2033条边、平均度值30.1,获得AKT1、IL-6、MAPK3等30个核心靶点蛋白。GO富集分析获得条目154个,其中10个富集最多,KEGG通路富集分析获得175条信号传导通路,其中16条富集显著性较高。结论:麻子仁丸可能是通过AGE-RAGE、TNF、IL-17、PI3K-Akt等信号通路控制AKT1、IL-6、MAPK3等靶点治疗功能性便秘,木犀草素可能发挥重要作用。展开更多
目的研究胃肠安丸(Weichang′an pill,WCA)、胃肠安丸醇提物(Ethanol extract of Weichang′an pill,EE)、胃肠安丸水提物(Water extract of Weichang′an pill,WE)及其活性成分对乙酰胆碱(Acetylcholine,ACh)诱导的大鼠离体回肠平滑肌...目的研究胃肠安丸(Weichang′an pill,WCA)、胃肠安丸醇提物(Ethanol extract of Weichang′an pill,EE)、胃肠安丸水提物(Water extract of Weichang′an pill,WE)及其活性成分对乙酰胆碱(Acetylcholine,ACh)诱导的大鼠离体回肠平滑肌收缩的影响及机制。方法采用离体组织浴实验,在ACh的作用下,加入WCA、EE、WE或其活性成分,记录离体大鼠回肠平滑肌的收缩张力;通过分子对接的方法探究活性成分与毒蕈碱型乙酰胆碱M3受体的结合亲和力。结果WCA、EE、WE均可明显抑制ACh诱导的回肠平滑肌兴奋性收缩。活性成分木香烃内酯、去氢木香内酯、檀香醇、麝香酮、大黄素、大黄酚、大黄素甲醚、巴豆苷、厚朴酚及和厚朴酚也对ACh诱导的回肠平滑肌收缩有明显的抑制作用。结论WCA、EE、WE及其活性成分可能通过阻断回肠平滑肌细胞膜上的M3受体与ACh的结合,发挥促进肠平滑肌松弛的作用。展开更多
文摘[Objectives]To analyze the effect of Mongolian pharmaceutical Betel Shisanwei Ingredient Pills in the clinical treatment of patients with depression.[Methods]From June 2020 to May 2021,64 patients with depression who received treatment in Inner Mongolia Minzu University were selected to participate in this study.These patients were randomly numbered from 1 to 64,and then divided into two groups according to the principle of odd or even number.Patients with odd number were regarded as the reference group,and treated by western medicine fluoxetine;patients with even number were regarded as the study group,and treated by Mongolian pharmaceutical Betel Shisanwei Ingredient Pills.The therapeutic effects of the two groups of patients were compared.[Results]Before treatment,there was no significant difference in the scores of patients'depressive syndromes between the two groups(P>0.05).After treatment,there were two significant changes in comprehensive score of depressive symptoms in both groups.Compared with before treatment,the data of the same group after treatment decreased significantly.Comparison between the two groups showed that the score of patients'depressive syndromes in the study group(13.28±5.49)was significantly lower than that in the reference group(18.46±6.51),and the overall response rate of treatment in the study group(96.88%,31/32)was obviously higher than that in the reference group(75.00%,24/32),showing statistically significant differences(P<0.05).[Conclusions]In the treatment of depression,Mongolian medicine therapy can significantly improve the depressive syndromes in patients,with more prominent curative effects,and is worthy of promotion and application.
文摘目的:通过网络药理学和分子对接方法探讨麻子仁丸治疗功能性便秘的作用机制。方法:在TCMSP数据库中检索麻子仁丸中各药物的主要活性成分及作用靶点,并应用UniProt数据库筛选功能性便秘的相关疾病靶点。应用Cytoscape 3.7.2软件构建有效活性成分-靶点网络;用STRING数据库构建PPI网络,基于Bioconductor数据库利用R 3.6.3软件进行基因本体论(gene ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析,并提取前20条结果生成可视化图形。结果:获取麻子仁丸的主要活性成分57个;作用靶点167个,功能性便秘相关基因4948个,交集基因135个。较重要的有效活性成分包括木犀草素、山柰酚、柚皮素等。构建可视化PPI网络图共得到135个节点、2033条边、平均度值30.1,获得AKT1、IL-6、MAPK3等30个核心靶点蛋白。GO富集分析获得条目154个,其中10个富集最多,KEGG通路富集分析获得175条信号传导通路,其中16条富集显著性较高。结论:麻子仁丸可能是通过AGE-RAGE、TNF、IL-17、PI3K-Akt等信号通路控制AKT1、IL-6、MAPK3等靶点治疗功能性便秘,木犀草素可能发挥重要作用。
文摘目的研究胃肠安丸(Weichang′an pill,WCA)、胃肠安丸醇提物(Ethanol extract of Weichang′an pill,EE)、胃肠安丸水提物(Water extract of Weichang′an pill,WE)及其活性成分对乙酰胆碱(Acetylcholine,ACh)诱导的大鼠离体回肠平滑肌收缩的影响及机制。方法采用离体组织浴实验,在ACh的作用下,加入WCA、EE、WE或其活性成分,记录离体大鼠回肠平滑肌的收缩张力;通过分子对接的方法探究活性成分与毒蕈碱型乙酰胆碱M3受体的结合亲和力。结果WCA、EE、WE均可明显抑制ACh诱导的回肠平滑肌兴奋性收缩。活性成分木香烃内酯、去氢木香内酯、檀香醇、麝香酮、大黄素、大黄酚、大黄素甲醚、巴豆苷、厚朴酚及和厚朴酚也对ACh诱导的回肠平滑肌收缩有明显的抑制作用。结论WCA、EE、WE及其活性成分可能通过阻断回肠平滑肌细胞膜上的M3受体与ACh的结合,发挥促进肠平滑肌松弛的作用。
