To date,the primary industrial carbon capture approach is still absorption using aqueous solutions of alkanolamines.Here,to pursue a substitute for the amine-based approach to improve the CO_(2) capture efficiency and...To date,the primary industrial carbon capture approach is still absorption using aqueous solutions of alkanolamines.Here,to pursue a substitute for the amine-based approach to improve the CO_(2) capture efficiency and decrease the energy cost further,we report a new carbon capture approach using a 2-methylimidazole(mIm)aqueous solution.The properties and sorption behaviors of this approach have been experimentally investigated.The results show that the mIm solution has higher CO_(2) absorption capacity under relatively higher equilibrium pressure(>130 kPa)and lower desorption heat than the methyldiethanolamine solution.91.6%sorption capacity of mIm solution can be recovered at 353.15 K and 80 kPa.The selectivity for CO_(2)/N_(2) and CO_(2)/CH_(4) can reach an exceptional 7609 and 4324,respectively.Furthermore,the pilot-scale tests were also performed,and the results demonstrate that more than 98%of CO_(2) in the feed gas could be removed and cyclic absorption capacity can reach 1 mol·L^(-1).This work indicates that mIm is an excellent alternative to alkanolamines for carbon capture in the industry.展开更多
In solving the deterioration of biological treatment system treating petrochemical wastewater under low temperatures, bioaugmentation technology was adopted by delivering engineering bacteria into a pilot-scale two-st...In solving the deterioration of biological treatment system treating petrochemical wastewater under low temperatures, bioaugmentation technology was adopted by delivering engineering bacteria into a pilot-scale two-stage anoxic-oxic (A/O) process based on previous lab-scale study. Experimental results showed that when the concentrations of COD and NH4+-N of the influent were 370~910 mg/L and 10~70 mg/L, the corresponding average concentrations of those of effluent were about 80 mg/L and 8 mg/L respectively, which was better than the Level I criteria of the Integrated Wastewater Discharge Standard (GB8978-1996). According to GC-MS analysis of the effluents from both the wastewater treatment plant (WWTP) and the pilot system, there were 68 kinds of persistent organic pollutants in the WWTP effluent, while there were only 32 in that of the pilot system. In addition, the amount of the organics in the effluent of the pilot system reduced by almost 50% compared to that of the WWTP. As a whole, after bioaugmentation, the organic removal efficiency of the wastewater treatment system obviously in- creased.展开更多
Under the guidance of strain tolerance, a new combination method for crude oil-degrading bacterial consortium was studied. Firstly, more than 50 efficient crude oil-degrading and biosurfactant producing bacteria were ...Under the guidance of strain tolerance, a new combination method for crude oil-degrading bacterial consortium was studied. Firstly, more than 50 efficient crude oil-degrading and biosurfactant producing bacteria were isolated from petroleum-contaminated soil and water in Tianjin Binhai New Area Oil field, China. Twenty-four of them were selected for further study. These strains were identified as belonging Pseudornonas aeruginosa, Bacillus subtilis, Brevibacillus brevis, Achrornobacter sp., Acinetobacter venetianus, Lysinibacillus rnacroides, Klebsiella oxytoca, Stenotrophornonas rhizophila, Rhodococcus sp. and Bacillus thuringiensis. A shake-flask degradation test revealed that 12 of these strains could degrade over 50% of 1% crude oil concentration in 7 d. Of these, 8 strains were able to produce biosurfactants. Furthermore, environmental tolerance experiments indicated that the majority of the strains had the ability to adapt to extreme environments including high temperatures, alkaline en- vironments and high salinity environments. A mixed bacterial agent comprising the strains WB2, W2, W3 and HA was developed based on the environmental tolerance tests and subjected to the pilot-scale degradation test indicating that this bacterial agent could degrade 85.2% of 0.8% crude oil concentration in 60 d. Our results suggest that the application of this mixed agent could remediate crude oil polluted soils in saline and alkaline environments.展开更多
[ Objective] This study aimed to explore the effect of C/N ratio on DHA production by Schizochytrium sp. , and the results were verified by pilot-scale fermentation. [ Method] Effect of C/N ratio on DHA production by ...[ Objective] This study aimed to explore the effect of C/N ratio on DHA production by Schizochytrium sp. , and the results were verified by pilot-scale fermentation. [ Method] Effect of C/N ratio on DHA production by Schizochytrium sp. JN-3 was explored. The industrial fermentation potential of Schizochytrium sp. JN-3 was investigated by pilot-scale fermentation in a 200 L fermentation tank. [ Result] Results of fermentation in a 7 L fermentation tank showed that the maximum biomass and total lipid content were 34.3 and 20.1 g/L, respectively, when C/N ratio was 10 ; the maximum DHA yield was 46.9% when C/N ratio was 20. When pilot-scale fermentation was conducted in a 200 L fermentation tank, the biomass was 30. 2 g/L, total lipid content was 18.4 g/L, accounting for 63.2% of the dry weight of cells; DHA content was 7.4 g/L, accounting for 40. 2% of the total lipid content. [ Condasion ] Schizochytrlum sp. JN-3 has good industrial fermentation notential.展开更多
基金The financial supports received from National Natural Science Foundation of China (U20B6005, 22178378, and 22127812)
文摘To date,the primary industrial carbon capture approach is still absorption using aqueous solutions of alkanolamines.Here,to pursue a substitute for the amine-based approach to improve the CO_(2) capture efficiency and decrease the energy cost further,we report a new carbon capture approach using a 2-methylimidazole(mIm)aqueous solution.The properties and sorption behaviors of this approach have been experimentally investigated.The results show that the mIm solution has higher CO_(2) absorption capacity under relatively higher equilibrium pressure(>130 kPa)and lower desorption heat than the methyldiethanolamine solution.91.6%sorption capacity of mIm solution can be recovered at 353.15 K and 80 kPa.The selectivity for CO_(2)/N_(2) and CO_(2)/CH_(4) can reach an exceptional 7609 and 4324,respectively.Furthermore,the pilot-scale tests were also performed,and the results demonstrate that more than 98%of CO_(2) in the feed gas could be removed and cyclic absorption capacity can reach 1 mol·L^(-1).This work indicates that mIm is an excellent alternative to alkanolamines for carbon capture in the industry.
基金Project supported by the National Basic Research Program (973) of China (No. 2004CB418505)the Science and Technology Development Program of Heilongjiang Province (No. CC05S301), China
文摘In solving the deterioration of biological treatment system treating petrochemical wastewater under low temperatures, bioaugmentation technology was adopted by delivering engineering bacteria into a pilot-scale two-stage anoxic-oxic (A/O) process based on previous lab-scale study. Experimental results showed that when the concentrations of COD and NH4+-N of the influent were 370~910 mg/L and 10~70 mg/L, the corresponding average concentrations of those of effluent were about 80 mg/L and 8 mg/L respectively, which was better than the Level I criteria of the Integrated Wastewater Discharge Standard (GB8978-1996). According to GC-MS analysis of the effluents from both the wastewater treatment plant (WWTP) and the pilot system, there were 68 kinds of persistent organic pollutants in the WWTP effluent, while there were only 32 in that of the pilot system. In addition, the amount of the organics in the effluent of the pilot system reduced by almost 50% compared to that of the WWTP. As a whole, after bioaugmentation, the organic removal efficiency of the wastewater treatment system obviously in- creased.
基金Supported by the National Basic Research Program of China(“973”Program:2014CB745100)the National Natural Science Foundation of China(21576197)+2 种基金Tianjin Research Program of Application Foundation and Advanced Technology(14JCQNJC06700)the Major Research Plan of Tianjin(16YFXTSF00460)Tianjin Penglai 19-3 Oil Spill Accident Compensation Project(19-3BC2014-03)
文摘Under the guidance of strain tolerance, a new combination method for crude oil-degrading bacterial consortium was studied. Firstly, more than 50 efficient crude oil-degrading and biosurfactant producing bacteria were isolated from petroleum-contaminated soil and water in Tianjin Binhai New Area Oil field, China. Twenty-four of them were selected for further study. These strains were identified as belonging Pseudornonas aeruginosa, Bacillus subtilis, Brevibacillus brevis, Achrornobacter sp., Acinetobacter venetianus, Lysinibacillus rnacroides, Klebsiella oxytoca, Stenotrophornonas rhizophila, Rhodococcus sp. and Bacillus thuringiensis. A shake-flask degradation test revealed that 12 of these strains could degrade over 50% of 1% crude oil concentration in 7 d. Of these, 8 strains were able to produce biosurfactants. Furthermore, environmental tolerance experiments indicated that the majority of the strains had the ability to adapt to extreme environments including high temperatures, alkaline en- vironments and high salinity environments. A mixed bacterial agent comprising the strains WB2, W2, W3 and HA was developed based on the environmental tolerance tests and subjected to the pilot-scale degradation test indicating that this bacterial agent could degrade 85.2% of 0.8% crude oil concentration in 60 d. Our results suggest that the application of this mixed agent could remediate crude oil polluted soils in saline and alkaline environments.
基金Supported by Research Fund for the Doctoral Program of Ministry of Education(20100093120002)Open Project of the Key Laboratory of Industrial Biotechnology under Ministry of Education(KLIB-KF200906,KLIB-KF201005)
文摘[ Objective] This study aimed to explore the effect of C/N ratio on DHA production by Schizochytrium sp. , and the results were verified by pilot-scale fermentation. [ Method] Effect of C/N ratio on DHA production by Schizochytrium sp. JN-3 was explored. The industrial fermentation potential of Schizochytrium sp. JN-3 was investigated by pilot-scale fermentation in a 200 L fermentation tank. [ Result] Results of fermentation in a 7 L fermentation tank showed that the maximum biomass and total lipid content were 34.3 and 20.1 g/L, respectively, when C/N ratio was 10 ; the maximum DHA yield was 46.9% when C/N ratio was 20. When pilot-scale fermentation was conducted in a 200 L fermentation tank, the biomass was 30. 2 g/L, total lipid content was 18.4 g/L, accounting for 63.2% of the dry weight of cells; DHA content was 7.4 g/L, accounting for 40. 2% of the total lipid content. [ Condasion ] Schizochytrlum sp. JN-3 has good industrial fermentation notential.
