The rooting capacity of Pinus massoniana is poor,especially for mature trees,and has prevented the development of clonal forestry for P.massoniana.In this study,we varied explant types,subculture times and exogenous h...The rooting capacity of Pinus massoniana is poor,especially for mature trees,and has prevented the development of clonal forestry for P.massoniana.In this study,we varied explant types,subculture times and exogenous hormones for plantlet regeneration and assessed shoots for rooting rate and root number for P.massoniana.Following fi ve repetitive grafts,new shoots from grafts used as explant sources were rejuvenated as observed from juvenile shoot morphology and anatomy,leading to greatly enhanced plant regeneration in comparison to that of mature materials from 26-year-old P.massoniana trees.The rooting capacity of subcultured shoots increased with successive subcultures,reaching a peak at 20 subcultures with 35–40 days per subculture.However,rooting performance was signifi cantly reduced after 30 subcultures.The addition of naphthaleneacetic acid(NAA)plus indoleacetic acid in the medium improved the root number,but the combination of exogenous NAA with paclobutrazol(PBZ)increased rooting rate and root number.We thus greatly improved the rooting capacity of mature P.massoniana trees by optimizing explant types(rejuvenated),subculture times(20 subcultures,35–40 days per subculture)and addition of NAA+PBZ to the rooting medium.The conditions can be used for effi cient plantlet regeneration of P.massoniana.展开更多
基金the Project of Scientifi c and Technological Plan from the Department of Science and Technology of Guangxi under Grants 2018GXNSFDA281020,AD17195078,2017GXNSFAA198037 and AA17204087-1the Natural Science Foundation of China under Grant 31960311 and 31360178the Key Program of Guangxi Forestry Bureau under Grant[2016]13 and GL2019KT06.
文摘The rooting capacity of Pinus massoniana is poor,especially for mature trees,and has prevented the development of clonal forestry for P.massoniana.In this study,we varied explant types,subculture times and exogenous hormones for plantlet regeneration and assessed shoots for rooting rate and root number for P.massoniana.Following fi ve repetitive grafts,new shoots from grafts used as explant sources were rejuvenated as observed from juvenile shoot morphology and anatomy,leading to greatly enhanced plant regeneration in comparison to that of mature materials from 26-year-old P.massoniana trees.The rooting capacity of subcultured shoots increased with successive subcultures,reaching a peak at 20 subcultures with 35–40 days per subculture.However,rooting performance was signifi cantly reduced after 30 subcultures.The addition of naphthaleneacetic acid(NAA)plus indoleacetic acid in the medium improved the root number,but the combination of exogenous NAA with paclobutrazol(PBZ)increased rooting rate and root number.We thus greatly improved the rooting capacity of mature P.massoniana trees by optimizing explant types(rejuvenated),subculture times(20 subcultures,35–40 days per subculture)and addition of NAA+PBZ to the rooting medium.The conditions can be used for effi cient plantlet regeneration of P.massoniana.
