Our previous studies indicated an increase in extracellular y-aminobutyric acid （GABA） in rodent＇s ischemic brain after Piroxicam administration, leading to alleviation of glutamate me- diated excitotoxicity throug...Our previous studies indicated an increase in extracellular y-aminobutyric acid （GABA） in rodent＇s ischemic brain after Piroxicam administration, leading to alleviation of glutamate me- diated excitotoxicity through activation of type A GABA receptor （GABAA）. This study was to investigate if GABAA activation by Piroxicam affects extracellular 5-hydroxytryptamine or not. High performance liquid chromatography revealed that there was a significant decrease in extracellular 5-hydroxytryptamine release in ischemic cerebral cortex and striatum in Piroxicam pre-treated rat brains. This suggests a probable role of Piroxicam in reducing extracellular 5-hy- droxytryptamine release in ischemic cerebral cortex and striatum possibly due to the GABAA activation by Piroxicam.展开更多
A simple and reliable strategy was proposed to engineer the glutathione grafted graphene oxide/ZnO nanocomposite(glutathione-GO/ZnO)as electrode material for the high-performance piroxicam sensor.The prepared glutathi...A simple and reliable strategy was proposed to engineer the glutathione grafted graphene oxide/ZnO nanocomposite(glutathione-GO/ZnO)as electrode material for the high-performance piroxicam sensor.The prepared glutathione-GO/ZnO nanocomposite was well characterized by X-ray diffraction(XRD),Fourier transform infrared spectrum(FTIR),X-ray photoelectron spectroscopy(XPS),field emission scanning electron microscopy(FE-SEM),cyclic voltammetry(CV),electrochemical impedance spectroscopy(EIS)and differential pulse voltammetry(DPV).The novel nanocomposite modified electrode showed the highest electrocatalytic activity towards piroxicam(oxidation potential is 0.52 V).Under controlled experimental parameters,the proposed sensor exhibited good linear responses to piroxicam concentrations ranging from 0.1 to 500 μM.The detection limit and sensitivity were calculated as 1.8 μM and 0.2 μA/μM·cm^(2),respectively.Moreover,it offered excellent selectivity,reproducibility,and long-term stability and can effectively ignore the interfering candidates commonly existing in the pharmaceutical tablets and human fluids even at a higher concentration.Finally,the reported sensor was successfully employed to the direct determination of piroxicam in practical samples.展开更多
Piroxicam is a non-steroidal anti-inflammatory drug (NSAID) exhibiting analgesic and antipiretic properties and widely used in the management of chronic diseases. Associated with these use, there are registers of adve...Piroxicam is a non-steroidal anti-inflammatory drug (NSAID) exhibiting analgesic and antipiretic properties and widely used in the management of chronic diseases. Associated with these use, there are registers of adverse reactions. Microparticle formulations in hydrogel matrix can be used to form a semi-permeable barrier which enables their actions and can reduce the effects. This work presents a study on the effect of gelatin/carboxymethyl cellulose (Gel/CMC) semi-IPN matrix composition on the retention and kinetic behavior of releasing this drug. The microparticles were obtained through the emulsion-crosslinking method using 23 factorial planning and the piroxicam was added as solid particles. In order to characterize the interaction between matrix-active agent and quantification of the drug, the following techniques were applied: SEM, DSC and XRD. SEM micrographs revealed microparticles with regular and spherical shape and that in some compositions the drug is partially absorbed and not encapsulated. Beyond that DSC and XRD analyses indicate that the piroxicam remained in the matrixes, maintains the same crystalline form. The factorial planning analysis indicated that matrix obtained a maximum encapsulation efficiency (EE%) of 10.64% and became possible to create a response surface graph using a EE% as answer. In addition to this, release kinetics analyses demonstrated that the release process seems to be governed by distinctly kinetic models, considering the composition of the sample. In some samples the release can be driven by Fickinianan diffusion, others by anomalous transport or swelling.展开更多
A simple and fast capillary electrophoresis method has been developed to determine the amount of piroxicam loaded in a drug delivery system based on nanostructured lipid carriers（NLCs）.The entrapment efficiency of t...A simple and fast capillary electrophoresis method has been developed to determine the amount of piroxicam loaded in a drug delivery system based on nanostructured lipid carriers（NLCs）.The entrapment efficiency of the nanostructured lipid carrier was estimated by measuring the concentration of drug not entrapped in a suspension of NLC.The influence of different parameters on migration times,peak symmetry,efficiency and resolution was studied;these parameters included the pH of the electrophoretic buffer solution and the applied voltage.The piroxicam peak was obtained with a satisfactory resolution.The separation was carried out using a running buffer composed of 50 mM ammonium acetate and 13.75 mM ammonia at pH 9.The optimal voltage was 20 kV and the cartridge temperature was 20 ℃.The corresponding calibration curve was linear over the range of 2.7-5.4 μg/mL of NLC suspension.The reproducibility of migration time and peak area were investigated,and the obtained RSD%values（n = 5） were 0.99 and 2.13.respectively.展开更多
文摘Our previous studies indicated an increase in extracellular y-aminobutyric acid （GABA） in rodent＇s ischemic brain after Piroxicam administration, leading to alleviation of glutamate me- diated excitotoxicity through activation of type A GABA receptor （GABAA）. This study was to investigate if GABAA activation by Piroxicam affects extracellular 5-hydroxytryptamine or not. High performance liquid chromatography revealed that there was a significant decrease in extracellular 5-hydroxytryptamine release in ischemic cerebral cortex and striatum in Piroxicam pre-treated rat brains. This suggests a probable role of Piroxicam in reducing extracellular 5-hy- droxytryptamine release in ischemic cerebral cortex and striatum possibly due to the GABAA activation by Piroxicam.
基金supported by SERB(Science and Engineering Research Board),New Delhi,India[File.No:EMR/2014/000020].
文摘A simple and reliable strategy was proposed to engineer the glutathione grafted graphene oxide/ZnO nanocomposite(glutathione-GO/ZnO)as electrode material for the high-performance piroxicam sensor.The prepared glutathione-GO/ZnO nanocomposite was well characterized by X-ray diffraction(XRD),Fourier transform infrared spectrum(FTIR),X-ray photoelectron spectroscopy(XPS),field emission scanning electron microscopy(FE-SEM),cyclic voltammetry(CV),electrochemical impedance spectroscopy(EIS)and differential pulse voltammetry(DPV).The novel nanocomposite modified electrode showed the highest electrocatalytic activity towards piroxicam(oxidation potential is 0.52 V).Under controlled experimental parameters,the proposed sensor exhibited good linear responses to piroxicam concentrations ranging from 0.1 to 500 μM.The detection limit and sensitivity were calculated as 1.8 μM and 0.2 μA/μM·cm^(2),respectively.Moreover,it offered excellent selectivity,reproducibility,and long-term stability and can effectively ignore the interfering candidates commonly existing in the pharmaceutical tablets and human fluids even at a higher concentration.Finally,the reported sensor was successfully employed to the direct determination of piroxicam in practical samples.
文摘Piroxicam is a non-steroidal anti-inflammatory drug (NSAID) exhibiting analgesic and antipiretic properties and widely used in the management of chronic diseases. Associated with these use, there are registers of adverse reactions. Microparticle formulations in hydrogel matrix can be used to form a semi-permeable barrier which enables their actions and can reduce the effects. This work presents a study on the effect of gelatin/carboxymethyl cellulose (Gel/CMC) semi-IPN matrix composition on the retention and kinetic behavior of releasing this drug. The microparticles were obtained through the emulsion-crosslinking method using 23 factorial planning and the piroxicam was added as solid particles. In order to characterize the interaction between matrix-active agent and quantification of the drug, the following techniques were applied: SEM, DSC and XRD. SEM micrographs revealed microparticles with regular and spherical shape and that in some compositions the drug is partially absorbed and not encapsulated. Beyond that DSC and XRD analyses indicate that the piroxicam remained in the matrixes, maintains the same crystalline form. The factorial planning analysis indicated that matrix obtained a maximum encapsulation efficiency (EE%) of 10.64% and became possible to create a response surface graph using a EE% as answer. In addition to this, release kinetics analyses demonstrated that the release process seems to be governed by distinctly kinetic models, considering the composition of the sample. In some samples the release can be driven by Fickinianan diffusion, others by anomalous transport or swelling.
基金financial support of Universidad Nacional del Sur(24/Q054)Consejo Nacional de Investigaciones Cientificas y Tecnicas(CONICET)
文摘A simple and fast capillary electrophoresis method has been developed to determine the amount of piroxicam loaded in a drug delivery system based on nanostructured lipid carriers（NLCs）.The entrapment efficiency of the nanostructured lipid carrier was estimated by measuring the concentration of drug not entrapped in a suspension of NLC.The influence of different parameters on migration times,peak symmetry,efficiency and resolution was studied;these parameters included the pH of the electrophoretic buffer solution and the applied voltage.The piroxicam peak was obtained with a satisfactory resolution.The separation was carried out using a running buffer composed of 50 mM ammonium acetate and 13.75 mM ammonia at pH 9.The optimal voltage was 20 kV and the cartridge temperature was 20 ℃.The corresponding calibration curve was linear over the range of 2.7-5.4 μg/mL of NLC suspension.The reproducibility of migration time and peak area were investigated,and the obtained RSD%values（n = 5） were 0.99 and 2.13.respectively.
