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Isolated Microspore Culture and Plant Regeneration of Leaf Mustard(Brassica juncea(L.)Czern.et Coss.) 被引量:1
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作者 Hongli YANG Jingfeng HU +3 位作者 Xuezhong XU Jiangming HE Shuang SONG 《Agricultural Biotechnology》 CAS 2016年第6期10-12,15,共4页
Isolated micmspore culture was carried out with Brassica juncea to study the effects of sterilization methods, culture period of embryos, hormone formula and the concentration of aetivatod carbon on embryogenesis, and... Isolated micmspore culture was carried out with Brassica juncea to study the effects of sterilization methods, culture period of embryos, hormone formula and the concentration of aetivatod carbon on embryogenesis, and the concentration of NAA on induction of roots. The results showed that the embryogenesis on medi- um subjected to filter sterilization is better than medium subjected to autoclaved sterilization. The embryos cultured for 15 d had the highest plantlat regenexafion rate (52%). NLN-13 liquid medium including 0. 1mg/L 6-BA and 0.2 g/L activated carbon significantly improved the planflet regeneration rate. 1/2 MS inclu- ding 0.3 mg/L NAA had the highest plantlet regeneration rate ( 100% ). Key words Brassica juncea ; Isolated micmspore culture ; Embryogenesis ; Planflet regeneration 展开更多
关键词 Broassica juncea Isolated microspore culture EMBRYOGENESIS planflet regeneration
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