Cultural Transference of Plant and Animal Images in Shijing Translation

As one of the Confucian classics, Shijing （i~） occupies a pivotal position in the development of Chinese culture. This paper makes a comparative study on the English translation of plant and animal images in Shijing, trying to reveal the cultural connotations in them and to suggest some translation strategies for the correct transference of cultural implications in plant and animal images in the poems of this ancient book.

Synthesis of Fluorescent Carbon Quantum Dots and Their Application in the Plant Cell Imaging

Carbon quantum dots(CQDs) exhibit tremendous advantages for plant growth study due to its strong fluorescence and good biocompatibility. The fluorescent CQDs were synthesized by the onestep microwave method with the raw materials of citric acid(CA) and urea(UR), and expressed a unique green fluorescence with the optimal excitation wavelength of over 400 nm through adjusting the doping of N elements. It is demonstrated that CQDs can act as deliver media in plant and fluorescent probes for plant cell imaging through directly cultivated in the seedlings of melon and wheat, respectively. Based on the effects of the fluorescent CQDs on plants growth, we can further study the mechanisms of the ions transport in plants.

Study on Five-sense Landscape of Chu Ci Plants Based on NVivo

Chu Ci plants carry profound cultural connotation,and entrust with the poets’ rich emotional understanding and sensory experience.The plant-related sentences in Chu Ci were extracted by NVivo software,and the verses were analyzed and compared based on the five-sense theory.The five-sense images of plants in Chu Ci were extracted from the five aspects of vision,hearing,smell,touch and taste,and the five-sense landscape characteristics of Chu Ci plants were analyzed.The results showed that Chu Ci plants had rich five-sense images,profound artistic conception,magnificent momentum and bold imagination,and had good application potential in landscape architecture.This paper provides a reference for the landscape application of five-sense landscape of Chu Ci plants and a new idea for the inheritance and development of Chu Ci culture.

Design of an HPPD fluorescent probe and visualization of plant responses to abiotic stress

The upregulation of 4-hydroxyphenylpyruvate dioxygenase(HPPD)can protect plants from adverse abiotic stress.Therefore,studying the changes of HPPD under abiotic stress is extremely valuable.In this study,we employed a rational molecular design strategy to prepare an HPPD-responsive fluorescent probe consisting of a pyrene fluorophore,a linker and a benquitrione skeleton recognition moiety that functions via an aggregation–disaggregation sensing mechanism.As predicted,this probe exhibited an obvious turn-on fluorescence response towards HPPD.In addition,in vivo imaging demonstrated the excellent capability of this probe to track HPPD in Arabidopsis thaliana,and the dynamic changes in HPPD were monitored under different stress degrees of high temperature and Cadmium(II)ion(Cd^(2+))stress.This work provides an efficient design strategy for acquiring a noninvasive HPPD fluorescence probe,which could evaluate the degree of stress and be further employed for exploring the stress resistance mechanism in plants.

Image based leaf segmentation and counting in rosette plants

This paper proposes an efficient method to extract the leaf region and count the number of leaves in digital plant images.The plant image analysis plays a significant role in viable and productive agriculture.It is used to record the plant growth,plant yield,chlorophyll fluorescence,plant width and tallness,leaf area,etc.frequently and accurately.Plant growth is a major character to be analyzed among these plant characters and it directly depends on the number of leaves in the plants.In this paper,a new method is presented for leaf region extraction from plant images and counting the number of leaves.The proposed method has three steps.The first step involves a new statistical based technique for image enhancement.The second step involves in the extraction of leaf region in plant image using a graph based method.The third step involves in counting the number of leaves in the plant image by applying Circular Hough Transform(CHT).The proposed work has been experimented on benchmark datasets of Leaf Segmentation Challenge(LSC).The proposed method achieves the segmentation accuracy of 95.4%and it also achieves the counting accuracy of
0.7(DiC)and 2.3(|DiC|)for datasets(A1,A2 and A3),which are better than the state-of-the-art methods.

Development of bi-oligo(ethylene glycol)-functionalized fluorescent probe for two-photon and noninvasive imaging in Arabidopsis thaliana

Fluorescent probe is an important tool for investigation of biological events in plant tissues.However,the research on small-molecule based fluorescent probe for plant imaging still stays at the initial stage.In order to noninvasively achieve the subcellular information of Arabidopsis thaliana,a two-photon and fluorene based fluorophore with a bi-oligo(ethylene glycol)functional group was synthesized.Under the laser's irradiation at 750 nm,the subcellular structures of Arabidopsis thaliana including stomata and root tips were clearly observed without slicing,which displayed significant superiorities over traditional single-photon excitation microscopy.Subsequently,the above functionalized fluorophore was further modified with a recognition group(dipicolylamine)to form a smart fluorescent probe(LJTP1).As results,LJTP1 not only can sensitively detect Cu^(2+)/S^(2-)with fluorescence“on-off-on”response in vitro and in vivo,but also can achieve noninvasive imaging in Arabidopsis thaliana tissues under two-photon microscopy.We believe this study will open up a new mind for noninvasive imaging in plant tissues.

A Hg(Ⅱ)-specific probe for imaging application in living systems and quantitative analysis in environmental/food samples

Mercury ions are highly toxic and can accumulate along food chains in water,soil,crops and animals.Effective detection of mercury ions in various media is of great significance for maintaining the ecological environment and protecting people's health.In this work,a mercury ions specific fluorescent probe was developed by a simple one-step reaction of commercial substrates of 4-chloro-7-nitro-2,1,3-benzoxadiazole and 1-(2-aminoethyl)-4-methylpiperazine.Investigation on sensing behavior showed that this probe had high sensitivity and selectivity towards mercury ions.Furthermore,this probe could be used as a tool to track the level of mercury ions in living system.In living cells,the probe with green emission emitted a bright red fluorescence when it was bound to mercury ions.In Arabidopsis thaliana,similar red emission could be detected from the root tip and stalk when A.thaliana was grown in culture medium containing mercury ions.The imaging in zebrafish showed that mercury ions were mainly concentrated in the stomach and head of zebrafish.Especially,this probe could be applied in quantitative analysis of mercury ions in tap water,green tea,sea shrimp and soil.This work provided a practical tool for the detection of mercury ions in living systems and quantitative analysis in real samples.