We selected 450 patients with chronic and difficult diseases as receptors, and selected edible and medicinal plant combinations as biological signal donors, and randomly participated in a new experiment. After adjusti...We selected 450 patients with chronic and difficult diseases as receptors, and selected edible and medicinal plant combinations as biological signal donors, and randomly participated in a new experiment. After adjusting the patient’s biological field, the compensating bio information energy (CBE) high-tech is applied to transmit the signal of plant radiation to the patient’s body. The physical therapy of this health care mode has the characteristics of no contact, no drugs, no intervention, no toxic side effects, no pain, and no electromagnetic radiation and so on. Before and after the experiment, the main function test, cell function, cell biochemistry and body temperature of each patient were used for the same body control and data statistical analysis, and then the comprehensive integration method was used to evaluate the effect. We found that after 1 - 4 courses of treatment (7 - 28 dx 2h), the potential disease risk of patients was significantly reduced, the relevant medical indicators improved rapidly, and the cell function and symptoms improved simultaneously. The effective rate was up to 90%, the significant efficiency was up to 57%. This experiment shows that this new physical therapy can treat both symptoms and root causes, make chronic and difficult diseases self-healing and rehabilitation, and has no ethical problems. It also shows that information on plant health can play an important role in reversing cell aging and restoring cell function. Therefore, it opens up a new field of natural therapy, which can be called cell information therapy.展开更多
The continued emergence of herbicide-resistant weeds and the increasing labor costs are threatening the ability of growers to manage weeds and maintain profits.The smart farm with the advantage of non-invasive and hig...The continued emergence of herbicide-resistant weeds and the increasing labor costs are threatening the ability of growers to manage weeds and maintain profits.The smart farm with the advantage of non-invasive and high-efficiency operation plays an important role in increasing the sustainability of agricultural system as it can optimize crop inputs such as herbicides while preserving resources including soil and water.An automatic weed control system requires a sensing subsystem capable of detecting and distinguishing crop plants from weeds.The overlapping plants remain a challenge for successful detection of weeds.Crop plant signaling is a new robot-plant interaction technique that allows the visualization of exogenous fluorescent signals applied to crop plants for crop/weed identification.Based on all published articles in the leading edge of knowledge,a comprehensive review of the mushrooming crop plant signaling for discriminations of weeds and crops is highlighted.The discussion outlines the significant progress that has been made in developing new and more robust automated systems along with the current challenges and future prospects.This paper details the promise of crop plant signaling for accurate and automated plant recognitions in cropping systems.There is no doubt that this review is of great significance to scholars in related research field to study the solutions to real-time weed control.展开更多
Although magnesium(Mg) is one of the most important nutrients, involved in many enzyme activities and the structural stabilization of tissues, its importance as a macronutrient ion has been overlooked in recent decade...Although magnesium(Mg) is one of the most important nutrients, involved in many enzyme activities and the structural stabilization of tissues, its importance as a macronutrient ion has been overlooked in recent decades by botanists and agriculturists, who did not regard Mg deficiency(MGD) in plants as a severe health problem. However, recent studies have shown,surprisingly, that Mg contents in historical cereal seeds have markedly declined over time, and two thirds of people surveyed in developed countries received less than their minimum daily Mg requirement. Thus, the mechanisms of response to MGD and ways to increase Mg contents in plants are two urgent practical problems. In this review, we discuss several aspects of MGD in plants, including phenotypic and physiological changes, cell Mg2+homeostasis control by Mg2+transporters, MGD signaling, interactions between Mg2+and other ions, and roles of Mg2+in plant secondary metabolism. Our aim is to improve understanding of the influence of MGD on plant growth and development and to advance crop breeding for Mg enrichment.展开更多
Nitric oxide(NO),a versatile molecule,plays multiple roles in plant growth and development and is a key signaling molecule in plant response to abiotic stress.Nutrient management strategy is critical for abiotic stres...Nitric oxide(NO),a versatile molecule,plays multiple roles in plant growth and development and is a key signaling molecule in plant response to abiotic stress.Nutrient management strategy is critical for abiotic stress alleviation in plants.Sulfur(S) is important under stress conditions,as its assimilatory products neutralize the imbalances in cells created by excessive generation of reactive oxygen species(ROS).NO abates the harmful effects of ROS by enhancing antioxidant enzymes,stimulating S assimilation,and reacting with other target molecules,and regulates the expression of various stress-responsive genes under salt stress.This review focuses on the role of NO and S in responses of plants to salt stress,and describes the crosstalk between NO and S assimilation in salt tolerance.The regulation of NO and/or S assimilation using molecular biology tools may help crops to withstand salinity stress.展开更多
OBJECTIVE： To test if myricanone （02H2405）, a cyclic diarylheptanoid, has anticancer effects on two different cancer cell lines HeLa and PC3. The present study was conducted with a note on the drug-DNA interaction ...OBJECTIVE： To test if myricanone （02H2405）, a cyclic diarylheptanoid, has anticancer effects on two different cancer cell lines HeLa and PC3. The present study was conducted with a note on the drug-DNA interaction and apoptotic signalling pathway. METHODS： Several studies like cytotoxicity, nuclear damage, annexin-V-fluorescein isothiocyanate （FITC）/propidium iodide （PI）-Iabelled apoptotic assay and cell cycle arrest, immunoblot and reverse transcriptase-polymerase chain reaction （RT-PCR） were used following standard protocols. Circular dichroism （CD） spectroscopy was also done to evaluate whether myricanone effectively interacted with DNA to bring about conformational changes that could strongly inhibit the cancer cell proliferation. RESULTS： Myricanone showed a greater cytotoxic effect on PC3 cells than on HeLa cells. Myricanone promoted G0/G arrest in HeLa cells and S phase arrest in PC3 cells. Nuclear condensation and annexin V-FITC/PI studies revealed that myricanone promoted apoptotic cell death. CD spectroscopic data indicated that myricanone had an interaction with calf thymus DNA that changed DNA structural conformation. RT-PCR and immunoblot studies revealed that myricanone activated the apoptotic signalling cascades through down-regulation of transcription factors like nuclear factor-KB （NF-KB） （p65）, and signal transducers and activators of transcription 3 （STAT3）; cell cycle regulators like cyclin D1, and survivin and other signal proteins like Bcl-2 and up-regulation of Bax, caspase-9 and caspase-3. CONCLUSION： Myricanone induced apoptosis in both types of cancer cells by triggering caspase activation, and suppression of cell proliferation by down-regulation of NF-KB and STAT3 signalling cascades, which makes it a suitable candidate for possible use in the formulation of therapeutic alent for combatin cancer.展开更多
Modern agriculture is facing new challenges in which ecological and molecular approaches are being integrated to achieve higher crop yields while minimizing negative impacts on the environment. The application of biof...Modern agriculture is facing new challenges in which ecological and molecular approaches are being integrated to achieve higher crop yields while minimizing negative impacts on the environment. The application of biofertilzers could meet this requirement. Biofertilizer is a natural organic fertilizer that helps to provide all the nutrients required by the plants and helps to increase the quality of the soil with a natural microorganism environment. This paper reviewed the types of biofertilzers, the biological basic of biofertilizers in plant growth promotion. This paper also assayed the bidirectional information exchange between plant-microbes in rhizoshpere and the signal pathway of plant growth- promoting rhizobacteria (PGPR) and plant growth-promoting fungi (PGPF) in the course of plant infection. At last, the challenges of the application and the promising future of biofertilizers were also discussed.展开更多
文摘We selected 450 patients with chronic and difficult diseases as receptors, and selected edible and medicinal plant combinations as biological signal donors, and randomly participated in a new experiment. After adjusting the patient’s biological field, the compensating bio information energy (CBE) high-tech is applied to transmit the signal of plant radiation to the patient’s body. The physical therapy of this health care mode has the characteristics of no contact, no drugs, no intervention, no toxic side effects, no pain, and no electromagnetic radiation and so on. Before and after the experiment, the main function test, cell function, cell biochemistry and body temperature of each patient were used for the same body control and data statistical analysis, and then the comprehensive integration method was used to evaluate the effect. We found that after 1 - 4 courses of treatment (7 - 28 dx 2h), the potential disease risk of patients was significantly reduced, the relevant medical indicators improved rapidly, and the cell function and symptoms improved simultaneously. The effective rate was up to 90%, the significant efficiency was up to 57%. This experiment shows that this new physical therapy can treat both symptoms and root causes, make chronic and difficult diseases self-healing and rehabilitation, and has no ethical problems. It also shows that information on plant health can play an important role in reversing cell aging and restoring cell function. Therefore, it opens up a new field of natural therapy, which can be called cell information therapy.
文摘The continued emergence of herbicide-resistant weeds and the increasing labor costs are threatening the ability of growers to manage weeds and maintain profits.The smart farm with the advantage of non-invasive and high-efficiency operation plays an important role in increasing the sustainability of agricultural system as it can optimize crop inputs such as herbicides while preserving resources including soil and water.An automatic weed control system requires a sensing subsystem capable of detecting and distinguishing crop plants from weeds.The overlapping plants remain a challenge for successful detection of weeds.Crop plant signaling is a new robot-plant interaction technique that allows the visualization of exogenous fluorescent signals applied to crop plants for crop/weed identification.Based on all published articles in the leading edge of knowledge,a comprehensive review of the mushrooming crop plant signaling for discriminations of weeds and crops is highlighted.The discussion outlines the significant progress that has been made in developing new and more robust automated systems along with the current challenges and future prospects.This paper details the promise of crop plant signaling for accurate and automated plant recognitions in cropping systems.There is no doubt that this review is of great significance to scholars in related research field to study the solutions to real-time weed control.
基金supported by the Science Foundation of Zhejiang Sci-Tech University(grant 14042008-Y)the Zhejiang Provincial Natural Science Foundation of China(grant number LY13C130001)+1 种基金the Zhejiang Provincial Top Key Discipline of Biology(grant number 2012A03-C)the National Natural Science Foundation of China(grant number 81373908)
文摘Although magnesium(Mg) is one of the most important nutrients, involved in many enzyme activities and the structural stabilization of tissues, its importance as a macronutrient ion has been overlooked in recent decades by botanists and agriculturists, who did not regard Mg deficiency(MGD) in plants as a severe health problem. However, recent studies have shown,surprisingly, that Mg contents in historical cereal seeds have markedly declined over time, and two thirds of people surveyed in developed countries received less than their minimum daily Mg requirement. Thus, the mechanisms of response to MGD and ways to increase Mg contents in plants are two urgent practical problems. In this review, we discuss several aspects of MGD in plants, including phenotypic and physiological changes, cell Mg2+homeostasis control by Mg2+transporters, MGD signaling, interactions between Mg2+and other ions, and roles of Mg2+in plant secondary metabolism. Our aim is to improve understanding of the influence of MGD on plant growth and development and to advance crop breeding for Mg enrichment.
基金the Department of Science & Technology,New Delhi and research facilities in lab of NAK in the DBT-BUILDER programme(No.BT/PR4872/INF/22/150/2012) of Department of Biotechnology,New Delhi
文摘Nitric oxide(NO),a versatile molecule,plays multiple roles in plant growth and development and is a key signaling molecule in plant response to abiotic stress.Nutrient management strategy is critical for abiotic stress alleviation in plants.Sulfur(S) is important under stress conditions,as its assimilatory products neutralize the imbalances in cells created by excessive generation of reactive oxygen species(ROS).NO abates the harmful effects of ROS by enhancing antioxidant enzymes,stimulating S assimilation,and reacting with other target molecules,and regulates the expression of various stress-responsive genes under salt stress.This review focuses on the role of NO and S in responses of plants to salt stress,and describes the crosstalk between NO and S assimilation in salt tolerance.The regulation of NO and/or S assimilation using molecular biology tools may help crops to withstand salinity stress.
基金partially supported by a grant sanctioned to Prof.A.R.Khuda-Bukhsh,Department of Zoology, University of Kalyani,India,by Boiron Laboratories, Lyon,France
文摘OBJECTIVE： To test if myricanone （02H2405）, a cyclic diarylheptanoid, has anticancer effects on two different cancer cell lines HeLa and PC3. The present study was conducted with a note on the drug-DNA interaction and apoptotic signalling pathway. METHODS： Several studies like cytotoxicity, nuclear damage, annexin-V-fluorescein isothiocyanate （FITC）/propidium iodide （PI）-Iabelled apoptotic assay and cell cycle arrest, immunoblot and reverse transcriptase-polymerase chain reaction （RT-PCR） were used following standard protocols. Circular dichroism （CD） spectroscopy was also done to evaluate whether myricanone effectively interacted with DNA to bring about conformational changes that could strongly inhibit the cancer cell proliferation. RESULTS： Myricanone showed a greater cytotoxic effect on PC3 cells than on HeLa cells. Myricanone promoted G0/G arrest in HeLa cells and S phase arrest in PC3 cells. Nuclear condensation and annexin V-FITC/PI studies revealed that myricanone promoted apoptotic cell death. CD spectroscopic data indicated that myricanone had an interaction with calf thymus DNA that changed DNA structural conformation. RT-PCR and immunoblot studies revealed that myricanone activated the apoptotic signalling cascades through down-regulation of transcription factors like nuclear factor-KB （NF-KB） （p65）, and signal transducers and activators of transcription 3 （STAT3）; cell cycle regulators like cyclin D1, and survivin and other signal proteins like Bcl-2 and up-regulation of Bax, caspase-9 and caspase-3. CONCLUSION： Myricanone induced apoptosis in both types of cancer cells by triggering caspase activation, and suppression of cell proliferation by down-regulation of NF-KB and STAT3 signalling cascades, which makes it a suitable candidate for possible use in the formulation of therapeutic alent for combatin cancer.
文摘Modern agriculture is facing new challenges in which ecological and molecular approaches are being integrated to achieve higher crop yields while minimizing negative impacts on the environment. The application of biofertilzers could meet this requirement. Biofertilizer is a natural organic fertilizer that helps to provide all the nutrients required by the plants and helps to increase the quality of the soil with a natural microorganism environment. This paper reviewed the types of biofertilzers, the biological basic of biofertilizers in plant growth promotion. This paper also assayed the bidirectional information exchange between plant-microbes in rhizoshpere and the signal pathway of plant growth- promoting rhizobacteria (PGPR) and plant growth-promoting fungi (PGPF) in the course of plant infection. At last, the challenges of the application and the promising future of biofertilizers were also discussed.
