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Construction of Plant Virus Expression Vector pClYVV/CP/W and Expression of GFP
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作者 王振东 王晓华 +5 位作者 孟鹏 秦会权 郑新伟 刘芳普 薛立江 张敏 《Agricultural Science & Technology》 CAS 2009年第5期38-41,48,共5页
[ Objective] The study was to report the construction of plant virus expression vector pCIYVV/CP/W and the expression of green fluorescent protein(GFP) with pCIYVV/CP/W, and to develop effective plat virus vector fo... [ Objective] The study was to report the construction of plant virus expression vector pCIYVV/CP/W and the expression of green fluorescent protein(GFP) with pCIYVV/CP/W, and to develop effective plat virus vector for plant bioreactor to produce useful protein. [ Method] A section of multiple cloning sites among NIb/CP genes in pCIYVV genome and deoxyribonucleotide polylinker of cleavage recognition sequence containing viral protease Nla were cloned with infectivity full-length cDNA of clover yellow vein virus (CIYVV), and pCIYVV/CP/W vector was constructed, GFP gene was inserted into pCIyVV/CP/W to construct the pCIYVV/CP/W/GFP vector. The transcription situation of recombinant virus clone was detected by RT-PCR, and targeted gene products expressed by recombinant virus clone were detected with western blot (WB). [Result] The broad bean seedling inoculated with pCIYVV/CP/W/GFP expressed the same symptom as wild type CIYVV, morbidity was of 100%, the result showed that recombinant virus clone pCIYVV/CP/W/GFP didn't suppress, insertion of foreign gene didn't destroy the open reading frame of pCIYVV/CP/W. Foreign gene can keep living in F, progeny virus genorne steadily, recombinant virus clone pCIYVV/CP/W/GFP could steadily express GFP in progeny virus at least.[ Conclusion] The useful plant virus vector was provided for useful protein expressing. 展开更多
关键词 plant virus expression vector pCIYVV/CP/W pCIYVV/CP/W/GFP CONSTRUCTION EXPRESSION
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The molecular mechanism of efficient transmission of plant viruses in variable virus-vector-plant interactions 被引量:4
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作者 Xiaobin Shi Zhuo Zhang +3 位作者 Chi Zhang Xuguo Zhou Deyong Zhang Yong Liu 《Horticultural Plant Journal》 SCIE CSCD 2021年第6期501-508,共8页
Plant viruses are mainly transmitted by insect vectors in the non-persistent,semi-persistent,or persistent modes.In the non-persistent mode,plant viruses are retained in the stylets of their insect vectors.In the semi... Plant viruses are mainly transmitted by insect vectors in the non-persistent,semi-persistent,or persistent modes.In the non-persistent mode,plant viruses are retained in the stylets of their insect vectors.In the semi-persistent mode,plant viruses are carried to vector foreguts or salivary glands,but they cannot spread to salivary glands.In the persistent mode,plant viruses are retained in vector guts and can spread to salivary glands.In the non-persistent and semi-persistent modes,plant viruses are retained for a short time and cannot enter the hemolymph of insect vectors,whereas in the persistent mode,plant viruses are retained for a relatively long time and can be found in the hemolymph.Here,we reviewed recent studies that uncovered molecular mechanisms of how plant viruses manipulate host traits for efficient transmission by insect vectors.Normally,plants that are infected with viruses,regardless of the transmission mode,tend to release more attractive volatiles to vectors.However,plant defensive systems are regulated differently by viruses in these three modes.In the non-persistent mode,virus infections significantly induce plant defense responses,which probably trigger vectors(e.g.,winged aphids)to disperse and transmit viruses in a short time.In the semi-persistent mode,virus infections frequently suppress plant defense responses,resulting in an increase of vector population and facilitating viral transmissions during vector outbreaks.In the persistent mode,virus infections reduce plant defense responses and manipulate plant traits to become suitable feeding sites in a relatively long period of time.Understanding the underlying mechanisms of virus–vector–plant interactions will lay a foundation for preventing virus transmission. 展开更多
关键词 plant virus transmission Non-persistent Semi-persistent PERSISTENT
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Insect transmission of plant viruses: Multilayered interactions optimize viral propagation 被引量:2
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作者 Beatriz Dader Chrstiane Then +3 位作者 Edwige Berthelot Marie Ducousso James C. K. Ng Martin Drucker 《Insect Science》 SCIE CAS CSCD 2017年第6期929-946,共18页
By serving as vectors of transmission, insects play a key role in the infection cycle of many plant viruses. Viruses use sophisticated transmission strategies to overcome the spatial barrier separating plants and the ... By serving as vectors of transmission, insects play a key role in the infection cycle of many plant viruses. Viruses use sophisticated transmission strategies to overcome the spatial barrier separating plants and the impediment imposed by the plant cell wall. Interactions among insect vectors, viruses, and host plants mediate transmission by integrating all organizational levels, from molecules to populations. Best-examined on the molecular scale are two basic transmission modes wherein virus-vector interactions have been well characterized. Whereas association of virus particles with specific sites in the vector's mouthparts or in alimentary tract regions immediately posterior to them is required for noncirculative transmission, the cycle of particles through the vector body is necessary for circulative transmission. Virus transmission is also determined by interactions that are associated with changes in vector feeding behaviors and with alterations in plant host's morphology and/or metabolism that favor the attraction or deterrence of vectors. A recent concept in virus-host-vector interactions proposes that when vectors land on infected plants, vector elicitors and effectors "inform" the plants of the confluence of interacting entities and trigger signaling pathways and plant defenses. Simultaneously, the plant responses may also influence virus acquisition and inoculation by vectors. Over- all, a picture is emerging where transmission depends on multilayered virus-vector-host interactions that define the route of a virus through the vector, and on the manipulation of the host and the vector. These interactions guarantee virus propagation until one or more of the interactants undergo changes through evolution or are halted by environmental interventions. 展开更多
关键词 ARBOvirus DEFENSE host plant insect vector INTERACTIONS plant virus transmission
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HEMIPTERAN-TRANSMITTED PLANT VIRUSES: RESEARCH PROGRESS AND CONTROL STRATEGIES 被引量:2
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作者 Haijian HUANG Junmin LI +1 位作者 Chuanxi ZHANG Jianping CHEN 《Frontiers of Agricultural Science and Engineering》 2022年第1期98-109,共12页
About 80% of plant viruses are transmitted by specific insect vectors, especiallyhemipterans with piercing-sucking mouthparts. Many virus-transmitting insectsare also important crop pests that cause considerable losse... About 80% of plant viruses are transmitted by specific insect vectors, especiallyhemipterans with piercing-sucking mouthparts. Many virus-transmitting insectsare also important crop pests that cause considerable losses in crop production.This review summarizes the latest research findings on the interactions betweenplant viruses and insect vectors and analyzes the key factors affecting insecttransmission of plant viruses from the perspectives of insect immunity, insectfeeding, and insect symbiotic microorganisms. Additionally, by referring to thelatest applications for blocking the transmission of animal viruses, potentialcontrol strategies to prevent the transmission of insect-vectored plant virusesusing RNAi technology, gene editing technology, and CRISPR/Cas9 + gene-driventechnology are discussed. 展开更多
关键词 control strategies FEEDING IMMUNITY insect vector MICROORGANISM plant virus
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Bioinspired optical antennas: gold plant viruses
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作者 SoonGweon Hong Mi Yeon Lee +1 位作者 Andrew O Jackson Luke P Lee 《Light(Science & Applications)》 SCIE EI CAS CSCD 2015年第1期447-453,共7页
The ability to capture the chemical signatures of biomolecules(i.e.,electron-transfer dynamics)in living cells will provide an entirely new perspective on biology and medicine.This can be accomplished using nanoscale ... The ability to capture the chemical signatures of biomolecules(i.e.,electron-transfer dynamics)in living cells will provide an entirely new perspective on biology and medicine.This can be accomplished using nanoscale optical antennas that can collect,resonate and focus light from outside the cell and emit molecular spectra.Here,we describe biologically inspired nanoscale optical antennas that utilize the unique topologies of plant viruses(and thus,are called gold plant viruses)for molecular fingerprint detection.Our electromagnetic calculations for these gold viruses indicate that capsid morphologies permit high amplification of optical scattering energy compared to a smooth nanosphere.From experimental measurements of various gold viruses based on four different plant viruses,we observe highly enhanced optical cross-sections and the modulation of the resonance wavelength depending on the viral morphology.Additionally,in label-free molecular imaging,we successfully obtain higher sensitivity(by a factor of up to 10^(6))than can be achieved using similar-sized nanospheres.By virtue of the inherent functionalities of capsids and the plasmonic characteristics of the gold layer,a gold virus-based antenna will enable cellular targeting,imaging and drug delivery. 展开更多
关键词 molecular sensor NANOPHOTONICS optical antenna optical spectroscopy plant virus PLASMONICS plasmonic resonant energy transfer(PRET) surface enhanced Raman scattering(SERS)
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Perspectives on plant virus diseases in a climate change scenario of elevated temperatures
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作者 Wei‑An Tsai Christopher A.Brosnan +1 位作者 Neena Mitter Ralf G.Dietzgen 《Stress Biology》 2022年第1期223-248,共26页
Global food production is at risk from many abiotic and biotic stresses and can be affected by multiple stresses simultaneously.Virus diseases damage cultivated plants and decrease the marketable quality of produce.Im... Global food production is at risk from many abiotic and biotic stresses and can be affected by multiple stresses simultaneously.Virus diseases damage cultivated plants and decrease the marketable quality of produce.Importantly,the progression of virus diseases is strongly affected by changing climate conditions.Among climate-changing vari-ables,temperature increase is viewed as an important factor that affects virus epidemics,which may in turn require more efficient disease management.In this review,we discuss the effect of elevated temperature on virus epidem-ics at both macro-and micro-climatic levels.This includes the temperature effects on virus spread both within and between host plants.Furthermore,we focus on the involvement of molecular mechanisms associated with tempera-ture effects on plant defence to viruses in both susceptible and resistant plants.Considering various mechanisms proposed in different pathosystems,we also offer a view of the possible opportunities provided by RNA-based technologies for virus control at elevated temperatures.Recently,the potential of these technologies for topical field applications has been strengthened through a combination of genetically modified(GM)-free delivery nanoplat-forms.This approach represents a promising and important climate-resilient substitute to conventional strategies for managing plant virus diseases under global warming scenarios.In this context,we discuss the knowledge gaps in the research of temperature effects on plant-virus interactions and limitations of RNA-based emerging technologies,which should be addressed in future studies. 展开更多
关键词 Elevated temperatures virus epidemics plant virus control RNA-based approaches Nanoparticles
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An introductory review on the common brown leafhopper(Orosius orientalis):A new soybean pest
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作者 Tianhao Pei Xin Cui +1 位作者 Shusen Shi Yu Gao 《Oil Crop Science》 CSCD 2024年第3期198-203,共6页
Soybean pests are one of the major factors limiting yield improvement.With the expansion of area and changes in cropping patterns,a number of new pests have been identified in the main soybean production areas of Chin... Soybean pests are one of the major factors limiting yield improvement.With the expansion of area and changes in cropping patterns,a number of new pests have been identified in the main soybean production areas of China.The common brown leafhopper,Orosius orientalis,is a new pest associated with soybean stay-green virus that has been discovered on cultivated soybean crop in the Yellow-Huai-hai region of China in recent years.The polyphagous insect has a wide feeding range and infests a variety of important grain and cash crops.This paper presents the basic information,geographical distribution,hosts,damage characteristics,plant virus transmission,occurrence patterns,and prevention and control measures O.orientalis.This review also provides insights into integrated prevention and control of the genus Orosius as an insect vector. 展开更多
关键词 SOYBEAN LEAFHOPPER plant virus Insect vector Orosius orientalis
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污泥厌氧稳定化流程中肠道病毒的去除与归趋
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作者 詹咏 钱康 +5 位作者 陆宇涵 陈江峰 王慧 白建峰 王先恺 董滨 《上海理工大学学报》 CAS CSCD 北大核心 2024年第5期556-566,共11页
根据对典型肠道病毒的生物性研究,选取诺如病毒、腺病毒、轮状病毒作为研究对象,以安徽某污泥厂作为研究地点,污泥厂采用的是离心浓缩-水解-厌氧消化-机械脱水-太阳能干化技术,旨在评估不同污泥处理工艺对这些病毒去除效果的影响。本研... 根据对典型肠道病毒的生物性研究,选取诺如病毒、腺病毒、轮状病毒作为研究对象,以安徽某污泥厂作为研究地点,污泥厂采用的是离心浓缩-水解-厌氧消化-机械脱水-太阳能干化技术,旨在评估不同污泥处理工艺对这些病毒去除效果的影响。本研究利用实时荧光定量PCR技术,对污泥处理各阶段的病毒含量进行定量分析。研究表明,污泥厌氧消化是诺如病毒和腺病毒的去除效率最高的单元,其对数去除率分别为0.68 lg和0.61 lg,太阳能干化单元是轮状病毒的去除效率最高的单元,其对数去除率为0.73 lg。该污泥处理流程对诺如病毒、腺病毒、轮状病毒总量的去除分别达到了98.7%,97.6%,98.4%,但由于进泥中病毒总量较高,最终污泥处置进入环境中含量为1.52×10^(4) copies/g DW,5.17×10^(4) copies/g DW,7.92×10^(3) copies/g DW。 展开更多
关键词 肠道病毒 污泥厂 厌氧消化 去除率
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Sugarcane Transcript Profiling Assessed by cDNA-AFLP Analysis during the Interaction with Sugarcane Mosaic Virus
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作者 Cibele N.F.Medeiros Marcos C.Goncalves +5 位作者 Ricardo Harakava Silvana Creste Paula M.Nóbile Luciana R.Pinto Dilermando Perecin Marcos G.A.Landell 《Advances in Microbiology》 2014年第9期511-520,共10页
Sugarcane mosaic caused by Sugarcane Mosaic Virus (SCMV) is one of the most important virus diseases of sugarcane. In the present study, changes in the transcription profile obtained by cDNA-AFLP analysis were investi... Sugarcane mosaic caused by Sugarcane Mosaic Virus (SCMV) is one of the most important virus diseases of sugarcane. In the present study, changes in the transcription profile obtained by cDNA-AFLP analysis were investigated in two sugarcane varieties contrasting to SCMV resistance, when challenged with a severe virus strain. Healthy plants derived from meristem tip tissue culture were mechanically inoculated under greenhouse controlled conditions and sampled at 24, 48 and 72 hours after inoculation. A total of 392 transcript-derived fragments (TDFs) were verified in the resistant variety against 380 in the susceptible one. The two sugarcane genotypes showed differential behavior in the number of induced and repressed TDFs along the time-course samplings. Ten out of 23 sequenced TDFs (unique from the resistance variety), showed identity with known plant sequences, mostly related to plant defense mechanisms against pathogens. The cDNA-AFLP technique was effective in revealing changes in the transcription profile within and between contrasting varieties when challenged by SCMV. 展开更多
关键词 plant Resistance plant viruses Molecular Markers Candidate Genes CDNA-AFLP SCMV
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Plant virology in the 21st century in China:Recent advances and future directions 被引量:3
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作者 Jianguo Wu Yongliang Zhang +14 位作者 Fangfang Li Xiaoming Zhang Jian Ye Taiyun Wei Zhenghe Li Xiaorong Tao Feng Cui Xianbing Wang Lili Zhang Fei Yan Shifang Li Yule Liu Dawei Li Xueping Zhou Yi Li 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2024年第3期579-622,共44页
Plant viruses are a group of intracellular pathogens that persistently threaten global food security.Significant advances in plant virology have been achieved by Chinese scientists over the last 20 years,including bas... Plant viruses are a group of intracellular pathogens that persistently threaten global food security.Significant advances in plant virology have been achieved by Chinese scientists over the last 20 years,including basic research and technologies for preventing and controlling plant viral diseases.Here,we review these milestones and advances,including the identification of new crop-infecting viruses,dissection of pathogenic mechanisms of multiple viruses,examination of multilayered interactions among viruses,their host plants,and virus-transmitting arthropod vectors,and in-depth interrogation of plantencoded resistance and susceptibility determinants.Notably,various plant virus-based vectors have also been successfully developed for gene function studies and target gene expression in plants.We also recommend future plant virology studies in China. 展开更多
关键词 China emerging plant viruses plant virology plant resistance/susceptibility to viral infection plant-virus transmission vector interactions plant virus-based vectors viral pathogenesis
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某化工园区综合废水处理单元中病毒群落的分布特征 被引量:1
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作者 梅红 汪炎 +1 位作者 洪文清 宋立岩 《工业用水与废水》 CAS 2023年第6期13-17,54,共6页
为了了解化工园区综合废水处理厂病毒群落的分布特征以及其与宿主(细菌)的关系,研究从某化工园区综合废水处理厂的原水、厌氧池和活性污泥中采集了9个样品,以宏基因组技术结合生物信息学分析了病毒群落在上述3个单元的分布特征。研究结... 为了了解化工园区综合废水处理厂病毒群落的分布特征以及其与宿主(细菌)的关系,研究从某化工园区综合废水处理厂的原水、厌氧池和活性污泥中采集了9个样品,以宏基因组技术结合生物信息学分析了病毒群落在上述3个单元的分布特征。研究结果表明Uroviricota门是污水处理厂中的优势病毒(53.42%),厌氧池中病毒相对丰度相较于进水中下降了29.75%,但是活性污泥中却上升了9.87%;原水、厌氧池和活性污泥中差异病毒的功能和感染对象均不同,表明污水处理厂中的病毒群落分布具有空间异质性;病毒群落的相似性会随着细菌群落丰富度(P=0.001)和多样性的增加而增加,表明宿主(细菌)对病毒结构具有重要影响。 展开更多
关键词 污水处理厂 病毒 细菌 宏基因组 厌氧池 活性污泥
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Roles of RNA m^(6)A modifications in plant-virus interactions
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作者 Hao He Mingxuan Jia +2 位作者 Jie Liu Xueping Zhou Fangfang Li 《Stress Biology》 2023年第1期621-624,共4页
Viral RNAs have been known to contain N^(6)-methyladenosine(m^(6)A)modifications since the 1970s.The function of these modifications remained unknown until the development of genome-wide methods to map m^(6)A residues... Viral RNAs have been known to contain N^(6)-methyladenosine(m^(6)A)modifications since the 1970s.The function of these modifications remained unknown until the development of genome-wide methods to map m^(6)A residues.Increasing evidence has recently revealed a strong association between m^(6)A modifications and plant viral infection.This highlight introduces advances in the roles of RNA m^(6)A modifications in plant-virus interactions. 展开更多
关键词 plant virus N^(6)-methyladenosine(m^(6)A)RNA modification plant-virus interactions
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植物病毒病媒介昆虫的传毒特性和机制研究进展 被引量:23
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作者 史晓斌 谢文 张友军 《昆虫学报》 CAS CSCD 北大核心 2012年第7期841-848,共8页
植物病毒病是农作物的"癌症",至今缺少有效的防治方法。目前已知80%的植物病毒病依赖于媒介昆虫传播,而媒介昆虫对植物病毒的传播是一个昆虫、病毒、寄主植物互作的过程,历经获毒、持毒和传毒等多个阶段,昆虫体内一系列病毒... 植物病毒病是农作物的"癌症",至今缺少有效的防治方法。目前已知80%的植物病毒病依赖于媒介昆虫传播,而媒介昆虫对植物病毒的传播是一个昆虫、病毒、寄主植物互作的过程,历经获毒、持毒和传毒等多个阶段,昆虫体内一系列病毒受体或蛋白参与了这个过程。昆虫传播病毒的方式有口针携带式、前肠保留式和体内循环式3类,它们各自对应的持久性为非持久性、半持久性和持久性,不同昆虫获取这3类病毒的获毒时间、在体内存留位置和传毒时间也各不相同。这个过程受到媒介昆虫的性别及龄期、寄主植物、环境条件、昆虫体内共生菌等多种因素的影响。与之相关的蛋白主要有病毒衣壳蛋白(CP)、次要衣壳蛋白(CPm)、GroEL蛋白、辅助因子(HC)和下颚口针蛋白等。近年来对植物病毒基因组的研究也取得了很大的进展,对昆虫传毒机制的研究正受到越来越广泛的关注。本文综述了近年来该领域内的相关研究进展,包括昆虫传播植物病毒的传毒方式、影响传毒效率的因素、传毒机制特别是昆虫体内与病毒传播可能相关的受体等。 展开更多
关键词 植物病毒 媒介昆虫 传毒 病毒受体 分子生物学 共生菌
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闽江流域福州过境段水体病毒污染调查分析 被引量:10
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作者 郑耀通 林奇英 谢联辉 《中国环境监测》 CAS CSCD 北大核心 2004年第5期39-43,共5页
自1998年11月至2000年4月,监测了闽江福州段病毒污染与水质状况。肠道病毒阳性率71 1%,平均病毒浓度5 33pfu L,植物病毒检出率32 2%,阳性率水样平均浓度0 3006枯斑 升,其中三个污染控制断面肠道内病毒阳性检出率及病毒浓度显著高于对照... 自1998年11月至2000年4月,监测了闽江福州段病毒污染与水质状况。肠道病毒阳性率71 1%,平均病毒浓度5 33pfu L,植物病毒检出率32 2%,阳性率水样平均浓度0 3006枯斑 升,其中三个污染控制断面肠道内病毒阳性检出率及病毒浓度显著高于对照和消减断面,揭示闽江福州段水环境病毒污染水平同福州市内河生活污水排放有直接关系,而引水内河冲污工程的实施显然又加剧了闽江水病毒污染的程度,增加了饮用水的卫生微生物学危险性。而水体环境中的植物病毒对农业生产存在着潜在的威胁。 展开更多
关键词 闽江水体 肠道病毒 植物病毒 病毒污染 污染监测
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用小RNA深度测序鉴定广西冬种马铃薯病毒 被引量:10
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作者 宋静静 蒙姣荣 +3 位作者 邹承武 李璞 王志强 陈保善 《中国农业科学》 CAS CSCD 北大核心 2013年第19期4075-4081,共7页
【目的】对广西冬种马铃薯病毒进行鉴定,为无病毒种薯选择和大田防治提供依据。【方法】2012年在马铃薯主要产区采集具有明显病毒病症状的样品,在血清学ELISA检测的基础上,进一步用小RNA深度测序对按症状分类的样品进行混合样本的病毒... 【目的】对广西冬种马铃薯病毒进行鉴定,为无病毒种薯选择和大田防治提供依据。【方法】2012年在马铃薯主要产区采集具有明显病毒病症状的样品,在血清学ELISA检测的基础上,进一步用小RNA深度测序对按症状分类的样品进行混合样本的病毒种类鉴定,再用RT-PCR方法对分组混合样本进行验证。【结果】在109个样本中检测到马铃薯卷叶病毒(Potato leafroll virus,PLRV)、马铃薯A病毒(Potato virus A,PVA)、马铃薯H病毒(Potato virus H,PVH)、马铃薯M病毒(Potato virus M,PVM)、马铃薯S病毒(Potato virus S,PVS)、马铃薯Y病毒(Potato virus Y,PVY)和马铃薯纺锤块茎类病毒(Potato spindle tuber viroid,PSTVd),同时发现PVS有丰富的株系分化。【结论】近年广西冬种马铃薯病毒种类增多,病症多样,亟需加强种薯管理,培育无毒健康种薯。 展开更多
关键词 小RNA高通量测序 广西 冬种马铃薯 病毒病害
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花椒根腐病的发生发展规律 被引量:9
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作者 朱天辉 陈第文 《四川农业大学学报》 CSCD 1995年第3期288-291,共4页
花椒根腐病的病原菌(Fusariumsolani)的菌丝生长以25-30℃、分生孢子萌发以20-30℃为最适宜,孢子在水滴中才能很好萌发,最适pH为5.6-7.0;病菌以菌丝和分生孢子主要从伤口浸入,主要以菌丝和厚垣孢子在土壤及其病根残体上越冬,一... 花椒根腐病的病原菌(Fusariumsolani)的菌丝生长以25-30℃、分生孢子萌发以20-30℃为最适宜,孢子在水滴中才能很好萌发,最适pH为5.6-7.0;病菌以菌丝和分生孢子主要从伤口浸入,主要以菌丝和厚垣孢子在土壤及其病根残体上越冬,一般4-5月开始侵染发病,6-8月最严重,10月下旬基本停止蔓延,其发病规律呈初发期、盛发期、停止期的单峰曲线。 展开更多
关键词 发病规律 花椒 腐皮镰孢菌 病害 防治 根腐病
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植物病毒的真菌传播及传毒介体油壶菌研究进展 被引量:3
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作者 蒋军喜 蔡祝南 《江西农业大学学报》 CAS CSCD 2003年第3期362-365,共4页
真菌传播植物病毒研究已成为植物病毒学研究的一个重要领域。扼要介绍了 40余年来 ,国内外关于植物病毒的真菌传播及传毒介体油壶菌的研究概况。
关键词 植物病毒 真菌传播 传毒介体 油壶菌
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斑点免疫测定法在植物病毒研究中的应用及技术要点 被引量:12
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作者 钱秀红 周雪平 《生物技术》 CAS CSCD 1994年第4期39-41,共3页
斑点免疫测定法(DIA)能快速、有效地检测出植物组织及种子中的病毒,且灵敏度高,不需特殊设备。本文介绍了DIA的基本方法及步骤,并重点论述了进行DIA测定的技术要点。
关键词 植物病毒 检测 斑点免疫测定
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真菌传播植物病毒的证据(综述) 被引量:3
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作者 陈剑平 阮义理 《浙江农业学报》 CSCD 1991年第4期202-205,共4页
(一)概述已知的传毒真菌都是一些植物根部专性寄生的游动孢子真菌;它们是壶菌目(Chytridiales)的油壶菌属(Olpidium)和集壶菌属(Synchytrium)以及根肿菌目(Plasmodiophorales)的多粘菌属(Pplymyxa)和粉痂菌属(Spongospora)。壶菌目游动... (一)概述已知的传毒真菌都是一些植物根部专性寄生的游动孢子真菌;它们是壶菌目(Chytridiales)的油壶菌属(Olpidium)和集壶菌属(Synchytrium)以及根肿菌目(Plasmodiophorales)的多粘菌属(Pplymyxa)和粉痂菌属(Spongospora)。壶菌目游动孢子仅具1根鞭毛,在寄主植物根中所形成的休眠孢子是单个的,而根肿菌目游动孢子具有2根鞭毛,并形成休眠孢子堆。两类真菌介体生活史的详细情况,特别是所谓的核融合(Karyogamy)尚不能肯定,但其总的发育过程是相似的;即在寄主根部形成单个或成堆的厚壁休眠孢子,当根腐烂后,休眠孢子(堆) 展开更多
关键词 真菌 植物病毒 传播 证据 真菌介体
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植物丛枝症病害病毒病原研究概况 被引量:1
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作者 杨根华 谭业明 +2 位作者 吴建宇 陈海如 张修国 《山东农业大学学报(自然科学版)》 CSCD 北大核心 2001年第2期261-266,共6页
对引起植物丛枝病害病毒的的分类地位、生物学特性、抗原特性、电镜观察和基因组成作了综合论述。
关键词 植物丛枝病 病毒 种类 症状 传播途径 实验寄主 抗原特性
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