Levels of serum leptin,cholecystokinin,plasma lipid and lipoprotein differ between patients with gallstone or/and those with hepatolithiasis

BACKGROUND: A significant relationship exists among food intake and nutritional status and cholelithiasis, including gallstone and hepatolithiasis. Leptin is associated with obesity. This study was to investigate the differences in serum leptin levels in patients with gallstone and hepatolithiasis and to evaluate the relationships among leptin, cholecystokinin (CCK), lipid and lipoprotein concentrations. METHODS: Body mass index (BMI), serum leptin, CCK, insulin, lipid and lipoprotein concentrations, and liver function were measured in 382 patients with gallstone (GS group), 83 patients with hepatolithiasis (HS group) and 30 healthy controls (control group). The values of these indices were compared among the groups. In each group, Pearson's product-moment correlation coefficient among these indices were evaluated. RESULTS: There were notable differences in serum leptin, CCK, total cholesterol, total triglycerides, apolipoprotein-a (APO-a), globulin, direct reacting bilirubin, and BMI between the GS and HS groups (P<0.05). Positive correlations between serum leptin and BMI, CCK, total cholesterol, gamma-glutamyl transpeptidase (GGT), aminotransferase, and insulin were found in the GS group (P<0.05). Positive correlations were observed between serum leptin and CCK, bilirubin, aminotransferase, GGT, in the HS group (P<0.05), but negative correlations between serum leptin and albumin or APO-a (P<0.05). CONCLUSIONS: Leptin participates in modulating lipid metabolism. There are notable differences in leptin, serum lipid, and CCK between patients with gallstone and those with hepatolithiasis. The role of leptin in the pathophysiological course of cholelithiasis needs further investigation.

Chemical Composition of the Fatty Oil from Fructus Broussonetiae and Its Effects on Rat Plasma Lipids and Adipose Tissue

Context: Broussonetia papyrifera (L.) Vent. (Moraceae), a traditional Chinese medicinal herb, has been extensively applied for many years to treat various diseases. Its fruits (Fructus Broussonetiae) have been commonly used as an important tonic for the treatment of age-related disorders with long history;recent research has proved that it contains 32% to 35% fixed oils. The fixed oil is composed mainly of unsaturated fatty acids, including linoleic acid, methyl palmitate, oleic acid and linoleic acid ester. Objective: To investigate the chemistry of the fatty oil from Fructus Broussonetiae (FOFB) and its effects on plasma lipids. Methods: The chemical composition of FOFB was examined and identified by GC-MS. Thirty male Wistar rats fed diet containing FOFB and cholesterol were studied for 28 days. The effect of dietary FOFB on plasma lipids and adipose tissue was tested. Results: Twelve compounds of FOFB were examined and identified, the major components of fatty oil, 8,11-octadecadienoic acid (83.75%), palmitic acid (10.22%), octadecadienoic acid (2.97%) and 9-octadecenoic acid (1.69%) were found. FOFB significantly exhibited the activities of decreasing the rat adipose tissue weight, triacylglycerol, total cholesterol, and low-density lipoprotein cholesterol (LDL-C) concentrations while the rat body weight remained unchanged. Discussion: FOFB contained a large amount of PUFA which had the effect on reducing plasma lipids and adipose.

A new discovered ABCA1 gene polymorphisms and the association of ABCA1 SNPs with coronary artery disease and plasma lipids in Chinese population

Objective: Single nucleotide polymorphisms (SNP) of ATP-binding cassette transporter A1 (ABCA1) gene are related to plasma lipid and susceptibility to coronary artery disease (CAD). Our first goal was to screen all 50 coding regions of ABCA1 to find new SNPs. Our second goal was to investigate the frequency distribution of R1587K and M883I polymorphisms of ABCA1 gene, which are the variant occurred most frequently, in Chinese people and to evaluate their association with the CAD phenotype and plasma lipids. Methods: Single-strand conformation polymorphism (SSCP) and DNA sequence were used for confirming new SNP of ABCA1, and restriction fragment length polymorphism (RFLP) were applied for confirming genotypes of R1587K and M883I in 112 CAD cases and 108 healthy people. Results: We discovered a new ABCA1 SNP in Chinese population, which converse 233 amino acids from Methionine to Valine (M233V). This new ABCA1 SNP located in exon7, and might potentially modulate the biological function of lipid metabolism. For R1587K and M883I SNPs, the K allele and I allele frequency was 28.9%and 31.1%, respectively. The K allele at R1587K conferred lower mean values of HDL-C in a dose-dependent manner in both CAD patients and healthy people. However, 883I allele was not associated with plasma lipid level. Neither 1587KK nor 883II associated with increased risk of CAD. Conclusion: Our study finds a potential functional ABCA1 SNPs and revealed K allele of R1587K associated decreased HDL-C level in Chinese population.

Effect of Artemether Treatment on Plasma Lipid Profile in Malaria

This study was undertaken to assess the effect of artemether treatment on plasma lipid profile in malaria infection. While the importance of lipid to plasmodial infective processes and metabolism is being increasingly appreciated, little is known about the attendant effect of chemotherapy on plasma lipid profile. Thirty patients with uncomplicated malaria were chosen from two secondary health-care facilities in Yobe State, Nigeria with informed consents and ethical clearance. Based on predetermined inclusion criteria patients were given 3.2 mg/kg of artemether with 1.6 mg/kg on subsequent days for a total of five days. This was done after the collection of urine and blood samples for urinalysis, malaria parasite density count and serum lipid analysis. A follow-up was planned seven (7) days from first dose during when clinical assessment and repeat malaria parasite density count and serum lipid analysis were done. Data were analyzed with statistical package for social scientist and Microsoft Excel spread sheet while level of significance at p ≤ 0.05 was calculated using paired t-test. Serum HDL cholesterol concentration recorded a significant decline of 0.13 mmol/L from a pre-treatment mean concentration of 1.17 mmol/L (p < 0.04). Triglyceride, total cholesterol, LDL-cholesterol, VLDL-cholesterol showed increment or reductions that were not significant. The clinical cure rate was 50% and mean percentage reduction in parasitaemia was 52%. A possible explanation for this low cure rate could be resistance, unfavorable pharmacokinetic disposition or lack of full adherence. A trial with complete parasite clearance, possibly using artemisinin-based combinational therapy would provide a more compelling result.

Effects of Plasma Lipids and Statins on Cognitive Function

Objective： Dementia is the fourth most common cause of death in developed countries. The relationship between plasma lipids and cognitive function is complex and controversial. Due to the increasing life expectancy of the population, there is an urgent need to control vascular risk factors and to identify therapies to prevent and treat both cognitive impairment and dementia. Here, we reviewed the eff＇ects of plasma lipids and statins on cognitive function. Data Sources： We searched the PubMed database for research articles published through November 2017 with key words including ＂plasma lipids,＂ ＂hyperlipidemia,＂ ＂hypercholesterolemia,＂ ＂statins,＂ and ＂cognition function.＂ Study Selection： Articles were retrieved and reviewed to analyze the effects of plasma lipids and statins on cognitive function and the mechanisms underlying these effects. Results： Many studies have examined the relationship between plasma lipids and cognitive function, but no definitive conclusions can be drawn. The mechanisms involved may include blood-brain barrier injury, the influence on small blood vessels in the brain, the influence on amyloid deposition, and a neuroprotective effect. To date, most studies of statins and cognition have been observational, with few randomized controlled trials. Therefore, finn conclusions regarding whether mid- or long-term statin use affects cognition function and dementia remain elusive. However, increasing concern exists that statins may be a causative factor for cognitive problems. These adverse eff＇ects appear to be rare and likely represent a yet-to-be-defined vulnerability in susceptible individuals. Conclusions： The association between plasma lipids and cognition, the mechanism of the influence of plasma lipids on cognitive function, and the association between statins and cognitive function are complex issues and currently not fully understood. Future research aimed at identifying the mechanisms that underlie the effects of plasma lipids and statins on cognition will not only provide important insight into the causes and interdependencies of cognitive impairment and dementia, but also inspire novel strategies for treating and preventing these cognitive disorders.

Gender differences in the relationship between plasma lipids and fasting plasma glucose in non-diabetic urban Chinese popula- tion： a cross-section study

The association between dyslipidemia and elevated fasting glucose in type 2 diabetes is well known. In non-diabetes, whether this association still exists, and whether dyslipidemia is an independent risk factor for high fasting plasma glucose （FPG） levels are not clear. This cross-sectional study recruited 3460 non-diabetic Chinese subjects （1027 men, and 2433 women, aged 35-75 years old） who participated in a health survey. Men and women were classified into tertiles by levels of plasma lipids respectively. In women, the prevalence of impaired fasting glucose （IFG） was decreased with increased HDL-C. A stepwise increase in HDL-C was associated with decreasing FPG levels （lowest tertiles, FPG： 5.376 ± 0.018; middle tertiles, 5.324± 0.018; highest tertiles, 5.276±0.018mmol/L; P = 0.001）. Reversely, FPG levels increased from lowest tertiles to highest tertiles of LDL-C, TC, and TG. we found that women in the first tertile with lower HDL-C level had a 1.75-fold increase in risk of IFG compared with non-diabetic women in the third tertile with higher HDL-C level （OR： 1.75; 95% CI： 1.20-2.56）. In men, no significant association was found. We took age, BMI, waist/hip ratio, education, smoking, alcohol drinking, and physical exercise as adjusted variables. In Chinese non-diabetic women, dyslipidemia is independently associated with high levels of FPG; TG, HDL-C, and LDL-C are predictors of IFG independent of BMI and waist/hip ratio.

Effects of Different Extract of PolygonumMultiflorumon Plasma Lipid，Liver Cholesterol and Blood Viscosity

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Modulatory effect of pineapple peel extract on lipid peroxidation,catalase activity and hepatic biomarker levels in blood plasma of alcoholinduced oxidative stressed rats

Objective:To investigate the ability of the methanolic extract of pineapple peel to modulate alcohol-induced lipid peroxidation,changes in catalase activities and hepatic biochemical marker levels in blood plasma.Methods:Oxidative stress was induced by oral administration of ethanol(20%w/v) at a dosage of 5 niL/kg bw in rats.After 28 days of treatment,the rats were fasted overnight and sacrificed by cervical dislocation.Blood was collected with a 2 mL syringe by cardiac puncture and was centrifuged at 3000 rpm for 10 min.The plasma was analyzed to evaluate malondialdehyde(MDA),catalase activity,aspartate aminotransferase(AST),alkaline phosphatase(ALP) and alanine aminotransferase(ALT) concentrations.Results:Administration of alcohol caused a drastic increase(87.74%) in MDA level compared with the control.Pineapple peel extract significantly reduced the MDA level by 60.16%at 2.S mL/kg bw.Rats fed alcohol only had the highest catalase activity,treatment with pineapple peel extract at 2.5 mL/kg bw however, reduced the activity.Increased AST,ALP and ALT activities were observed in rats fed alcohol only respectively,treatment with pineapple peel extract drastically reduced their activities. Conclusions:The positive modulation of lipid peroxidation,catalase activities as well as hepatic biomarker levels of blood plasma by the methanolic extract of pineapple peels under alcoholinduced oxidative stress is an indication of its protective ability in the management of alcoholinduced toxicity.

Impact of anthocyanin from Malva sylvestris on plasma lipids and free radical

从血浆类脂化合物和自由的基上的 Malva sylvestris 的 anthocyanin 的效果被试剂工具包方法和试管内试金调查。高胖的模型带着从 Malva sylvesiris 用 anthocyanin 的不同剂量被喂的白化体老鼠被建立(0.03 g · d ^(-1), 0.04 g · d^(-1) 和 0.05 g · d^(-1)) 。结果证明全部的胆固醇被 19.7% 在 0.04 g · d~ 的 anthocyanin 减少(-1) 和 triglyceride 被 34.4% 在 0.05 g · d~ 的 anthocyanin 减少(-1) 。试管内试金， anthocyanin 的一些索引包括清除的能力被测量自由激进分子，减少的力量和由 orthophenanthroline Fe^(+2 ) 的 anti-lipidperoxidation 的能力氧化减小方法。结果显示清理当 anthocyanin 的内容是 0.20 mg · m L~ 时，联系到 43.46% 的自由激进分子评价(当内容是 0.5 mg · m L~ 时， -1) 和类脂化合物的抑制比率每氧化到达了 18.82%(-1) 。因此 anthocyanin 是一种天赋和有效免费激进的搬运工和抗氧化剂并且能阻止血栓的形成并且减少心血管的疾病的出现。

Plasma apo CⅢ Levels in Relation to Inflammatory Traits and Metabolic Syndrome in Patients not Treated with Lipid-lowering Drugs Undergoing Coronary Angiography

Objective Assessment of the comprehensive relationship among apolipoprotein CIII（apoCⅢ） levels, inflammation, and metabolic disorders is rare. Methods A total of 1455 consecutive patients not treated with lipid-lowering drugs and undergoing coronary angiography were enrolled in this cross-sectional study. A mediation analysis was used to detect the underlying role of apoCⅢ in the association of inflammation with metabolic syndrome（MetS）. Results Patients with MetS showed higher levels of apoCⅢ [95.1（73.1-131.4） vs. 81.7（58.6-112.4） μg/mL, P 〈 0.001] and inflammatory markers [high sensitivity C-reactive protein, 1.7（0.8-3.4） vs. 1.1（0.5-2.2） mg/L; white blood cell count,（6.48 ± 1.68） vs.（6.11 ± 1.67） × 10~9/L]. The levels of apoCⅢ and inflammatory markers increased with the number of metabolic risk components（all P 〈 0.001）. Furthermore, apoCⅢ levels were associated with virtually all individual MetS risk factors and inflammatory markers（all P 〈 0.05）. Importantly, the prevalence of MetS in each metabolic disorder rose as apoCⅢ levels increased（all P 〈 0.05）. Mediation analysis showed that apoCⅢ partially mediated the effect of inflammation on MetS independently from triglycerides. Conclusion Plasma apoCⅢ levels were significantly associated with the development and severity of MetS, and a role of apoCⅢ in the effect of inflammation on the development of MetS was identified.

The Effect of Atmospheric Pressure Plasma Corona Discharge on pH, Lipid Content and DNA of Bacterial Cells

Effect of plasma corona discharges on the pH, whole cell lipids and DNA of bacteria is investigated. Results showed an increase in the acidity levels of water due to plasma reactive species which, however, were not responsible for bacterial cell death. No changes in the whole cell lipid contents were observed, while DNA after plasma treatment showed deterioration of the amplified sequences, indicating the possible occurrence of DNA degradation. In conclusion, reactive species produced by plasma discharges affects DNA, possibly contributing to cell death.

Not All “BAD” Cholesterol Carriers Are Necessarily Bad and Not All “GOOD” Cholesterol Carriers Are as Good as Can Be: Plasma Delipidation, a Non-Pharmacological Treatment for Atherosclerosis

More than four decades ago it was established that an elevated low-density lipoprotein-cholesterol level was a risk for developing coronary artery disease. For the last two decades, statins have been the cornerstone of reducing low-density lipoprotein-cholesterol, but despite significant clinical efficacy in the majority of patients, a large number of patients suffer from side effects and cannot tolerate the required statin dose to reach their recommended low-density lipoprotein-cholesterol goals. Preliminary clinical studies indicate that monoclonal antibodies to PCSK9 appear to be highly efficacious in lowering low-density lipoprotein-cholesterol with a favourable adverse event profile. However, further longer-term clinical studies are required to determine their safety. From the early-proposed concept for high-density lipoprotein-mediated cholesterol efflux for the treatment of coronary artery disease, the concentration of the cholesterol content in high-density lipoprotein particles has been considered a surrogate measurement for the efficacy of the reverse cholesterol transport process. However, unlike the beneficial effects of the statins and monoclonal antibodies to PCSK9 in reducing low-density lipoprotein-cholesterol, no significant advances have been made to increase the levels of high-density lipoprotein-cholesterol. Here it is shown that by a non-pharmacological plasma delipidation means, the atherogenic low-density lipoproteins can be converted to anti-atherogenic particles and that the high-density lipoproteins are converted to particles with extreme high affinity to cause rapid regression of atherosclerosis.

Effectiveness of Apricots (<i>Prunus armeniaca</i>), Pomegranate (<i>Punica granatum</i>) Juice and Lactic Acid Fermented Sobya on Plasma Levels of Lipid Profile Parameters and Total Homocysteine among Egyptian Adults

Objectives: The aim of this study was to investigate the effectiveness of dietary intervention with apricots, pomegranate juice or lactic acid fermented sobya on improving the plasma levels of lipid profile parameters and total plasma homocysteine (tHcy) among healthy adults. Design of the study: Thirty-five men and women (28.9 ± 3.0 years) with mean body mass index of 23.5 ± 1.2 kg/m2 were randomly assigned to a dietary intervention study. During the pre-feeding study records of 24-hour food intake, anthropometric measurements and blood sampling were collected for biochemical investigations. Thereafter the volunteers were divided into five groups: a control group (C);the other four groups received daily one of the following supplements: 200 g of deep yellow apricots (Prunus armeniaca) (AF);250 g pomegranate (Punica granatum) juice (PJ);mixture of 100 g (PJ) and 150 g of fermented sobya (FS), (PJ-FS) or 165 g (FS). After completing the three-week dietary intervention, blood samples were collected and blood indices were again evaluated. Biochemical analysis of total lipids (TL), total glycerides (TG), total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) and tHcy were completed. The low density lipoprotein-cholesterol level and three indices of risk factors were calculated using the appropriate equations. Results: Three-week dietary intervention improved the levels of lipid profile parameters TG, TC, HDL-C and LDL-C as compared to the respective baseline levels and the response of the control group that did not receive any supplement. Consuming (PJ-FS) had a synergistic beneficially added value on some of the above mentioned lipid profile parameters. The mean plasma tHcy concentrations;a marker for occlusive vascular disease was reduced significantly following the three week consumption of (PJ) or (FS), compared with the respective pre-feeding levels. Conclusion: Pomegranate juice (PJ) or lactic acid fermented sobya (FS) or mixture of both are potential natural agents in lowering the plasma levels of lipid profile parameters and tHcy among apparently healthy young women and men, without dyslipidemic symptoms.

Effects of salinity on activities of H^+-ATPase, H^+-PPase and membrane lipid composition in plasma membrane and tonoplast vesicles isolated from soybean(Glycine max L.) seedlings

The effects of NaCl stress on the H +-ATPase, H +-PPase activity and lipid composition of plasma membrane(PM) and tonoplast(TP) vesicles isolated from roots and leaves of two soybean cultivars(Glycine max L.) differing in salt tolerance(Wenfeng7, salt-tolerant; Union, salt-sensitive) were investigated. When Wenfeng7 was treated with 0.3%(W/V) NaCl for 3 d, the H +-ATPase activities in PM and TP from roots and leaves exhibited a reduction and an enhancement, respectively. The H +-PPase activity in TP from roots also increased. Similar effects were not observed in roots of Union. In addition, the increases of phospholipid content and ratios of phospholipid to galactolipid in PM and TP from roots and leaves of Wenfeng7 may also change membrane permeability and hence affect salt tolerance.

Plasma Induced Changes in Human Lipid Composition as Revealed through XPS-Analysis

In this study two plasma sources were used for an in vivo treatment of human stratum corneum. The sample preparation was realised with the Cyanoacrylat stripping method, whereby a few layers of corneocytes embedded in the lipid matrix were removed from the skin of healthy volunteers. For the plasma treatment, dielectric barrier discharges with pulse durations in the microsecond as well as in the nanosecond range were applied. A comparison of these sources with respect to their biologically active components including dissipated power, gas and electron temperature, irradiance in the ultraviolet range, ozone and nitric oxide concentration is presented. Furthermore, species generated during plasma treatment on the sample surface like hydrogen peroxide, nitride or nitrate were measured using reflectometry. In addition, safety aspects for both sources were evaluated. Resulting plasma induced changes in the sample composition were investigated through X-ray photoelectron spectroscopy. The main ingredients carbon, oxygen, and nitrogen in addition to minor concentrations of sulphur were considered. A significant influence of the pulse duration on plasma characteristics was shown. A more effective formation of reactive species as well as more intense UV emission for ns-plasma was observed. Based on the determined parameters, both plasma sources are suitable for therapeutic purpose. Furthermore, significant plasma induced changes in the stratum corneum composition were reported, including an increase in nitrogen and oxygen content.

冷等离子体技术对肉品品质的影响研究进展

肉类及其产品在运输、贮藏等过程中常常受到微生物的污染而导致食用价值的降低和食源性疾病的发生。冷等离子体(cold plasma,CP)技术作为一种新型非热杀菌技术在肉品中得到广泛应用,主要通过产生的活性氮和活性氧对肉品进行杀菌进而达到杀菌保藏的目的,但其产生的各种活性粒子会对肉品的品质造成不同程度的影响。该文综述了CP技术的基本原理,从肉品脂质氧化、色泽、嫩度、风味、pH值5个方面详细阐述了CP技术对肉品品质的影响,同时探讨了其在肉品杀菌保藏中的应用,以期为CP技术在肉和肉制品中的应用和推广提供理论参考。

去脂血浆净化治疗急性高脂血症性胰腺炎的效果分析

目的:分析去脂血浆净化治疗急性高脂血症性胰腺炎的临床效果。方法:选取2020年1月—2022年9月东台市人民医院收治的急性高脂血症性胰腺炎患者85例,按照随机数字表法分为研究组和对照组,对照组(42例)使用血浆置换治疗,研究组(43例)则采用去脂血浆净化血液灌流治疗,比较两组临床疗效。结果:研究组治疗总有效率、高密度脂蛋白胆固醇(HDL-C)、转化生长因子-β(TGF-β)水平和平均动脉压(MAP)均高于对照组,三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、淀粉酶(AMS)、降钙素原(PCT)、C反应蛋白(CRP)、白介素-6(IL-6)水平均低于对照组,腹痛消失时间早于对照组,住院时间短于对照组,差异均有统计学意义(P<0.05)。结论:去脂血浆净化应用于血液灌流治疗急性高脂血症性胰腺炎效果显著,能够有效稳定患者血脂水平,同时改善其免疫功能。

妊娠早期血炎症指标、空腹血糖及血脂对妊娠期糖尿病的联合预测价值

目的探讨妊娠早期(孕6~13^(+6)周)孕妇血炎症指标、空腹血糖及血脂与妊娠期糖尿病(GDM)的关系。方法选择2020年11月至2021年10月在新乡医学院第一附属医院进行产检的98例妊娠早期孕妇为研究对象,依据受试者妊娠中期(孕24~28周)的口服葡萄糖耐量试验结果将其分为GDM组(n=35)和糖耐量正常(NGT)组(n=63)。所有受试者于孕6~13^(+6)周时空腹8 h以上,于第2天清晨抽取肘正中静脉血,测定白细胞(WBC)计数、中性粒细胞计数(NC)、淋巴细胞计数(LC)、单核细胞计数(MC)、空腹血糖(FPG)及血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平,比较GDM组与NGT组孕妇各指标之间的差异。应用多因素logistic回归模型寻求联合预测因子并进行GDM发生的独立危险因素分析,采用受试者操作特征(ROC)曲线评估各危险因素预测GDM发生的效能。结果GDM组孕妇妊娠早期FPG、WBC计数、LC、TC、TG、LDL-C水平显著高于NGT组(P<0.05);GDM组与NGT组孕妇的NC、MC及HDL-C水平比较差异无统计学意义(P>0.05)。Logistic回归模型分析显示,FPG、WBC计数、TC、TG升高是GDM发生的独立危险因素(P<0.05)。以FPG=4.80 mmol·L^(-1)、WBC=9.35×10^(9)L^(-1)、TC=4.05 mmol·L^(-1)、TG=1.61 mmol·L^(-1)为截断值,预测GDM的ROC曲线下面积(AUC)分别为0.779、0.721、0.685、0.762,敏感度分别为0.886、0.514、0.857、0.543,特异度分别为0.587、0.857、0.524、0.873,四者联合预测GDM的AUC为0.876,灵敏度为0.857,特异度为0.810。妊娠早期FPG、WBC计数、TC、TG联合预测GDM的AUC高于FPG、WBC、TC、TG单独预测GDM的AUC。结论妊娠早期(孕6~13^(+6)周)孕妇血FPG、WBC计数、TC、TG是GDM的独立危险因素,可作为早期预测GDM发生的临床指标,且4项指标联合对GDM有更好的预测价值。

益气化聚方与硫辛酸治疗2型糖尿病的血糖、代谢及神经病变分析

目的:观察益气化聚方联合硫辛酸对2型糖尿病(T2DM)患者血糖、代谢指标及周围神经病变的影响。方法:选取2020年8月至2022年12月上海中医药大学附属岳阳中西医结合医院收治的T2DM患者120例作为研究对象,采用随机数字表法分为对照组和观察组,每组60例。对照组给予硫辛酸治疗,观察组在对照组基础上加用益气化聚方治疗。比较2组患者在治疗后的临床疗效、血糖变化、代谢相关指标、周围神经病变及治疗期间不良反应发生情况。结果:治疗后,观察组患者治疗总有效率高于对照组,且高低密度脂蛋白胆固醇(HDL-C)水平、总神经运动神经传导速度(MNCV)及感觉神经传导速度(SNCV)评分(正中神经和腓总神经)显著升高,差异有统计学意义(P<0.05),空腹血糖(FPG)、餐后2 h血糖(2 h PG)、总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、胰岛素抵抗指数(HOMA-IR)与对照组比较显著降低(P<0.05)。观察组治疗期间出现血小板功能异常2例、脸色苍白1例,对照组治疗期间出现血小板功能异常2例、脸色苍白2例,2组治疗后不良反应发生情况差异无统计学意义。结论:采用益气化聚方联合硫辛酸治疗T2DM可更明显改善周围神经病变,降低血糖,控制血脂,且安全性良好。

脂血对血常规红细胞参数测定结果的干扰及处理方法

目的:探讨脂血对血常规红细胞参数测定结果的干扰及处理方法。方法:选取2021年9月—2023年8月在广西壮族自治区脑科医院行血常规检测且血脂异常增高的脂血标本128份,分析采用等量0.9%氯化钠溶液置换血浆处理前后血常规参数变化。结果:处理后,血红蛋白(HGB)、平均血红蛋白量(MCH)、平均血红蛋白浓度(MCHC)、血小板(PLT)水平低于处理前,差异有统计学意义(P<0.05);处理前后,白细胞(WBC)、红细胞(RBC)、红细胞压积(HCT)、红细胞平均体积(MCV)比较,差异无统计学意义(P>0.05);在128份脂血标本中,23份MCHC≥380g/L,占17.97%,105份MCHC<380g/L,占82.03%。处理后,350g/L≤MCHC<380g/L和MCHC≥380g/L组的HGB水平均低于处理前,差异有统计学意义(P<0.05);处理前后,MCHC<350g/L组的HGB水平比较,差异无统计学意义(P>0.05)。结论:当MCHC≥350g/L时,建议检查标本性状,若是脂血影响,可通过等量0.9%氯化钠溶液置换血浆法进行纠正,0.9%氯化钠溶液置换血浆法能有效降低脂血对血常规红细胞参数测定结果的干扰,为临床和患者提供准确的检验报告。