A new phosphorylcholine, (6-hydroxy) hexyl-2-(trimethylammonio) ethyl phosphate (HTEP), was synthesized andcharasterized. Segmented polyurethane (SPU) containing phosphorylcholine structure was synthesized based ondip...A new phosphorylcholine, (6-hydroxy) hexyl-2-(trimethylammonio) ethyl phosphate (HTEP), was synthesized andcharasterized. Segmented polyurethane (SPU) containing phosphorylcholine structure was synthesized based ondiphenylmethane diisocyanate (MDI), soft segment polytetramethylene glycol (PTMG) and HTEP, with 1,4-butanediol (BD)as a chain extender. The existence of phosphorylcholine structure on the surface of SPU was revealed by attenuated totalreflectance Fourier transform infrard spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and water contactangle measurements. The blood compatibilities of the polymers were evaluated by hemolytic testing and a platelet-richplasma (PRP) adhesion experiment, which was viewed by scanning electron microscopy (SEM) with polyurethane as areference. The novel segmented polyurethane containing phosphorylcholine structure showed improved blood compatibility.展开更多
Zwitterionic phosphobetaine bearing a hydroxyl and a zwitterionic group,8-hydroxy-2-octyl phosphorylcholine（HOPC）,was synthesized and constructed to the surface of silk fibroin（SF） films in order to improve the he...Zwitterionic phosphobetaine bearing a hydroxyl and a zwitterionic group,8-hydroxy-2-octyl phosphorylcholine（HOPC）,was synthesized and constructed to the surface of silk fibroin（SF） films in order to improve the hemocompatibility of fibroin films by a an isocyanate head group.The surface characteristics of the modified films were measured by attenuated total reflection Fourier transform infrared spectroscopy（ATR-FTIR） and electron spectroscopy for chemical analysis（ESCA）,displaying the successful immobilization of Zwitterionic phosphobetaine on the surface of these fibroin films.Moreover,the further platelet adhesion test in platelets rich plasma（PRP） of human beings showed the zwitterionic phosphobetaine led mainly to good nonthrombogenicity.The experimental results indicated a reasonable approach to improve the blood compatibility of fibroin films.展开更多
目的探索AB型单采血小板次侧相容性输注后,对后期患者同型血小板输注疗效的影响。方法收集2020年11月至2021年9月本院血小板输注2276人次,其中ABO同型血小板输注2068人次,AB型次侧相容性输注206人次,选出第1次AB次侧相容性输注117人次,A...目的探索AB型单采血小板次侧相容性输注后,对后期患者同型血小板输注疗效的影响。方法收集2020年11月至2021年9月本院血小板输注2276人次,其中ABO同型血小板输注2068人次,AB型次侧相容性输注206人次,选出第1次AB次侧相容性输注117人次,AB次侧相容性输注前同型血小板输注为对照组,AB型次侧相容性输注后同型血小板输注为试验组(第1次输注为第1组,第2次为第2组,第3次为第3组),以血小板输注前、后患者血小板计数(Plt)、血小板增高值(△Plt)以及24 h Plt纠正增加指数(CCI)为观测指标,按性别、血型和病种分析AB型次侧相容的非同型血小板输注对后期同型血小板预防性输注疗效的影响。结果2276人次血小板输注,同型血小板输注△Plt明显高于AB型次侧相容性输注,其平均值分别为(14±15)×10^(9)/L、(11±14)×10^(9)/L,P<0.05;女性患者输注血小板后△Plt高于男性患者,分别为(15±16)×10^(9)/L、(13±14)×10^(9)/L,P<0.05;病种MDS、NHL和MM血小板输注疗效差于其它病种,△Plt(9±14、10±12、8±11)×10^(9)/L,明显低于平均值(P<0.05)。117例AB型次侧相容性输注:AB型次侧相容性血小板输注后△Plt明显低于对照组和试验组,分别为(9±12)×10^(9)/L、(13±13)×10^(9)/L、(13±12)×10^(9)/L,P<0.05;24 h CCI有效率为52.14%明显低于对照组(71.59%)、试验组(71.83%),P<0.05。试验组与对照组△Plt、24 h CCI有效例数均差异不明显,P>0.05。试验组第3组△Plt(10±13)×10^(9)/L相较于第1组(14±12)×10^(9)/L明显有所降低,P<0.05,有效输注率也有所下降,由73.12%(68/93)降至58.70%(27/46),P>0.05。试验组和对照组分别从性别、血型、病种三方面因数进行对比,△Plt、24 h CCI有效例数均差异不明显,P>0.05。结论AB型次侧相容性血小板输注△Plt和有效率低于同型血小板输注,对患者短期(10 d)内的同型血小板输注影响不明显。展开更多
基金Supported by the Special Funds for Major State Basic Research Projects (G1999064705)
文摘A new phosphorylcholine, (6-hydroxy) hexyl-2-(trimethylammonio) ethyl phosphate (HTEP), was synthesized andcharasterized. Segmented polyurethane (SPU) containing phosphorylcholine structure was synthesized based ondiphenylmethane diisocyanate (MDI), soft segment polytetramethylene glycol (PTMG) and HTEP, with 1,4-butanediol (BD)as a chain extender. The existence of phosphorylcholine structure on the surface of SPU was revealed by attenuated totalreflectance Fourier transform infrard spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and water contactangle measurements. The blood compatibilities of the polymers were evaluated by hemolytic testing and a platelet-richplasma (PRP) adhesion experiment, which was viewed by scanning electron microscopy (SEM) with polyurethane as areference. The novel segmented polyurethane containing phosphorylcholine structure showed improved blood compatibility.
基金Funded by the National High Technology Research and Development Program(863) of China (2006AA03Z445)
文摘Zwitterionic phosphobetaine bearing a hydroxyl and a zwitterionic group,8-hydroxy-2-octyl phosphorylcholine（HOPC）,was synthesized and constructed to the surface of silk fibroin（SF） films in order to improve the hemocompatibility of fibroin films by a an isocyanate head group.The surface characteristics of the modified films were measured by attenuated total reflection Fourier transform infrared spectroscopy（ATR-FTIR） and electron spectroscopy for chemical analysis（ESCA）,displaying the successful immobilization of Zwitterionic phosphobetaine on the surface of these fibroin films.Moreover,the further platelet adhesion test in platelets rich plasma（PRP） of human beings showed the zwitterionic phosphobetaine led mainly to good nonthrombogenicity.The experimental results indicated a reasonable approach to improve the blood compatibility of fibroin films.
文摘目的探索AB型单采血小板次侧相容性输注后,对后期患者同型血小板输注疗效的影响。方法收集2020年11月至2021年9月本院血小板输注2276人次,其中ABO同型血小板输注2068人次,AB型次侧相容性输注206人次,选出第1次AB次侧相容性输注117人次,AB次侧相容性输注前同型血小板输注为对照组,AB型次侧相容性输注后同型血小板输注为试验组(第1次输注为第1组,第2次为第2组,第3次为第3组),以血小板输注前、后患者血小板计数(Plt)、血小板增高值(△Plt)以及24 h Plt纠正增加指数(CCI)为观测指标,按性别、血型和病种分析AB型次侧相容的非同型血小板输注对后期同型血小板预防性输注疗效的影响。结果2276人次血小板输注,同型血小板输注△Plt明显高于AB型次侧相容性输注,其平均值分别为(14±15)×10^(9)/L、(11±14)×10^(9)/L,P<0.05;女性患者输注血小板后△Plt高于男性患者,分别为(15±16)×10^(9)/L、(13±14)×10^(9)/L,P<0.05;病种MDS、NHL和MM血小板输注疗效差于其它病种,△Plt(9±14、10±12、8±11)×10^(9)/L,明显低于平均值(P<0.05)。117例AB型次侧相容性输注:AB型次侧相容性血小板输注后△Plt明显低于对照组和试验组,分别为(9±12)×10^(9)/L、(13±13)×10^(9)/L、(13±12)×10^(9)/L,P<0.05;24 h CCI有效率为52.14%明显低于对照组(71.59%)、试验组(71.83%),P<0.05。试验组与对照组△Plt、24 h CCI有效例数均差异不明显,P>0.05。试验组第3组△Plt(10±13)×10^(9)/L相较于第1组(14±12)×10^(9)/L明显有所降低,P<0.05,有效输注率也有所下降,由73.12%(68/93)降至58.70%(27/46),P>0.05。试验组和对照组分别从性别、血型、病种三方面因数进行对比,△Plt、24 h CCI有效例数均差异不明显,P>0.05。结论AB型次侧相容性血小板输注△Plt和有效率低于同型血小板输注,对患者短期(10 d)内的同型血小板输注影响不明显。
