Using the method of dual-wavelength measurement of platelet [Ca2+]i, and Fura-2 as the Ca2+ fluorophore probe, we measured the effect of acidic Mu-copolysaccharide from Sticopus Japonicus Selenka (SJAMP) on platelet [...Using the method of dual-wavelength measurement of platelet [Ca2+]i, and Fura-2 as the Ca2+ fluorophore probe, we measured the effect of acidic Mu-copolysaccharide from Sticopus Japonicus Selenka (SJAMP) on platelet [Ca2+]i. The results showed that the most significant increase in platelets [Ca2+]i was seen when the concentration of SJAMP was 100μg/ml and the elevation of normal platelet [Ca2+]i was 93. 96±10. 24 nmol/L (n = 10). In the presence of extracellular Ca2+(1 mmol/L), the magnitude of platelet [Ca2+]i response to SJAMP was increased and the [Ca2+]i, could reach 116. 72 + 10. 66 nmol/L (n= 10). On the other hand, the magnitude of increased platelet [Ca2+]i; induced by SJAMP was smaller and the duration of [Ca2+]i reaching the highest level was longer when compared with other platelet aggregation agents. In the mean time, if platelets were first incubated with cyclooxygenase inhibitor, the rise of [Ca2+]i evoked by SJAMP was inhibited. The results indicated that the mechanism of the rise of [Ca2+]i induced by SJAMP might be dependent upon the generation of prostaglandin endoperoxides and (or) TXA?.展开更多
基金This project was supported by grant from the National Nature Science Foundation of China (No.39370322)
文摘Using the method of dual-wavelength measurement of platelet [Ca2+]i, and Fura-2 as the Ca2+ fluorophore probe, we measured the effect of acidic Mu-copolysaccharide from Sticopus Japonicus Selenka (SJAMP) on platelet [Ca2+]i. The results showed that the most significant increase in platelets [Ca2+]i was seen when the concentration of SJAMP was 100μg/ml and the elevation of normal platelet [Ca2+]i was 93. 96±10. 24 nmol/L (n = 10). In the presence of extracellular Ca2+(1 mmol/L), the magnitude of platelet [Ca2+]i response to SJAMP was increased and the [Ca2+]i, could reach 116. 72 + 10. 66 nmol/L (n= 10). On the other hand, the magnitude of increased platelet [Ca2+]i; induced by SJAMP was smaller and the duration of [Ca2+]i reaching the highest level was longer when compared with other platelet aggregation agents. In the mean time, if platelets were first incubated with cyclooxygenase inhibitor, the rise of [Ca2+]i evoked by SJAMP was inhibited. The results indicated that the mechanism of the rise of [Ca2+]i induced by SJAMP might be dependent upon the generation of prostaglandin endoperoxides and (or) TXA?.
