BACKGROUND Critically sized bone defects represent a significant challenge to orthopaedic surgeons worldwide.These defects generally result from severe trauma or resection of a whole large tumour.Autologous bone graft...BACKGROUND Critically sized bone defects represent a significant challenge to orthopaedic surgeons worldwide.These defects generally result from severe trauma or resection of a whole large tumour.Autologous bone grafts are the current gold standard for the reconstruction of such defects.However,due to increased patient morbidity and the need for a second operative site,other lines of treatment should be introduced.To find alternative unconventional therapies to manage such defects,bone tissue engineering using a combination of suitable bioactive factors,cells,and biocompatible scaffolds offers a promising new approach for bone regeneration.AIM To evaluate the healing capacity of platelet-rich fibrin(PRF)membranes seeded with allogeneic mesenchymal bone marrow-derived stem cells(BMSCs)on critically sized mandibular defects in a rat model.METHODS Sixty-three Sprague Dawley rats were subjected to bilateral bone defects of critical size in the mandibles created by a 5-mm diameter trephine bur.Rats were allocated to three equal groups of 21 rats each.Group I bone defects were irrigated with normal saline and designed as negative controls.Defects of group II were grafted with PRF membranes and served as positive controls,while defects of group III were grafted with PRF membranes seeded with allogeneic BMSCs.Seven rats from each group were killed at 1,2 and 4 wk.The mandibles were dissected and prepared for routine haematoxylin and eosin(HE)staining,Masson's trichrome staining and CD68 immunohistochemical staining.RESULTS Four weeks postoperatively,the percentage area of newly formed bone was significantly higher in group III(0.88±0.02)than in groups I(0.02±0.00)and II(0.60±0.02).The amount of granulation tissue formation was lower in group III(0.12±0.02)than in groups I(0.20±0.02)and II(0.40±0.02).The number of inflammatory cells was lower in group III(0.29±0.03)than in groups I(4.82±0.08)and II(3.09±0.07).CONCLUSION Bone regenerative quality of critically sized mandibular bone defects in rats was better promoted by PRF membranes seeded with BMSCs than with PRF membranes alone.展开更多
Ischemic heart disease (IHD) is the most common cause of death around the world. Nowadays Platelet counts (PC) and volumetric platelet indices are available routinely in most laboratories and reflect the level of mobi...Ischemic heart disease (IHD) is the most common cause of death around the world. Nowadays Platelet counts (PC) and volumetric platelet indices are available routinely in most laboratories and reflect the level of mobility and production of platelets. It seems that the excessive flexibility and size of the platelets and their local activation have correlation with extent of ischemic heart disease. So our objective is the study of platelet indices in ischemic heart disease. This non-randomized prospective study was performed on 245 patients with ischemic heart disease, who underwent the coronary angiography. The patients were divided into four groups: stable angina, unstable angina, acute myocardial infarction and control group;and then platelet indices, including the platelet counts (PC), the average platelet volume (MPV), the Platelet Distribution Width (PDW) and plateletcrit (PCT) in each group with the extent of coronary disease were compared based on an Syntax Score system and observational methods. The average ages of the patients were 57 years and 65% of them were male and the rest were female. A significant difference is exists between indices in all three groups compared to the control that this difference was related to gender and the type of the coronary artery involvement. However, only in infarction group, PDW in different disease intensities was significantly different. In this study, unlike many of the previous studies no relationship was found between the MPV with the extent of coronary disease.展开更多
目的探讨术前血小板分布宽度(platelet distribution width,PDW)与肿瘤大小(tumor size,TS)比值(PDW to TS ratio,PdwTR)在非转移性肺腺癌患者无病生存期(disease-free survival,DFS)中的预测价值。方法收集解放军总医院海南医院自2012...目的探讨术前血小板分布宽度(platelet distribution width,PDW)与肿瘤大小(tumor size,TS)比值(PDW to TS ratio,PdwTR)在非转移性肺腺癌患者无病生存期(disease-free survival,DFS)中的预测价值。方法收集解放军总医院海南医院自2012年12月至2020年12月经手术治疗后病理确诊的非转移性肺腺癌患者198例。根据受试者工作特征(ROC)曲线确定PdwTR的最佳截断值,并根据截断值将患者分为PdwTR低组和PdwTR高组,采用时间依赖的ROC曲线评估PdwTR、PDW、TS预测DFS的效能,Cox比例风险模型分析PdwTR与DFS的关联。结果PdwTR最佳截点值为7.45,其预测3年DFS的敏感性为93.90%,特异性为61.20%,ROC曲线下面积(AUC)为0.83(95%CI:0.75~0.90)。Log-rank检验结果显示,PdwTR低组的DFS低于PdwTR高组(log-rankχ^(2)=28.99,P<0.001);Cox回归模型调整年龄、性别、BMI、CEA水平、T分期、N分期、TNM分期、NLR、LMR和PNI等潜在混杂因素后,PdwTR高组的复发风险较PdwTR低组下降93.0%(HR=0.07,95%CI:0.02~0.33,P=0.001)。结论术前PdwTR低与非转移性肺腺癌患者DFS较差独立相关,可作为DFS的预测指标。展开更多
文摘BACKGROUND Critically sized bone defects represent a significant challenge to orthopaedic surgeons worldwide.These defects generally result from severe trauma or resection of a whole large tumour.Autologous bone grafts are the current gold standard for the reconstruction of such defects.However,due to increased patient morbidity and the need for a second operative site,other lines of treatment should be introduced.To find alternative unconventional therapies to manage such defects,bone tissue engineering using a combination of suitable bioactive factors,cells,and biocompatible scaffolds offers a promising new approach for bone regeneration.AIM To evaluate the healing capacity of platelet-rich fibrin(PRF)membranes seeded with allogeneic mesenchymal bone marrow-derived stem cells(BMSCs)on critically sized mandibular defects in a rat model.METHODS Sixty-three Sprague Dawley rats were subjected to bilateral bone defects of critical size in the mandibles created by a 5-mm diameter trephine bur.Rats were allocated to three equal groups of 21 rats each.Group I bone defects were irrigated with normal saline and designed as negative controls.Defects of group II were grafted with PRF membranes and served as positive controls,while defects of group III were grafted with PRF membranes seeded with allogeneic BMSCs.Seven rats from each group were killed at 1,2 and 4 wk.The mandibles were dissected and prepared for routine haematoxylin and eosin(HE)staining,Masson's trichrome staining and CD68 immunohistochemical staining.RESULTS Four weeks postoperatively,the percentage area of newly formed bone was significantly higher in group III(0.88±0.02)than in groups I(0.02±0.00)and II(0.60±0.02).The amount of granulation tissue formation was lower in group III(0.12±0.02)than in groups I(0.20±0.02)and II(0.40±0.02).The number of inflammatory cells was lower in group III(0.29±0.03)than in groups I(4.82±0.08)and II(3.09±0.07).CONCLUSION Bone regenerative quality of critically sized mandibular bone defects in rats was better promoted by PRF membranes seeded with BMSCs than with PRF membranes alone.
文摘Ischemic heart disease (IHD) is the most common cause of death around the world. Nowadays Platelet counts (PC) and volumetric platelet indices are available routinely in most laboratories and reflect the level of mobility and production of platelets. It seems that the excessive flexibility and size of the platelets and their local activation have correlation with extent of ischemic heart disease. So our objective is the study of platelet indices in ischemic heart disease. This non-randomized prospective study was performed on 245 patients with ischemic heart disease, who underwent the coronary angiography. The patients were divided into four groups: stable angina, unstable angina, acute myocardial infarction and control group;and then platelet indices, including the platelet counts (PC), the average platelet volume (MPV), the Platelet Distribution Width (PDW) and plateletcrit (PCT) in each group with the extent of coronary disease were compared based on an Syntax Score system and observational methods. The average ages of the patients were 57 years and 65% of them were male and the rest were female. A significant difference is exists between indices in all three groups compared to the control that this difference was related to gender and the type of the coronary artery involvement. However, only in infarction group, PDW in different disease intensities was significantly different. In this study, unlike many of the previous studies no relationship was found between the MPV with the extent of coronary disease.
文摘目的探讨术前血小板分布宽度(platelet distribution width,PDW)与肿瘤大小(tumor size,TS)比值(PDW to TS ratio,PdwTR)在非转移性肺腺癌患者无病生存期(disease-free survival,DFS)中的预测价值。方法收集解放军总医院海南医院自2012年12月至2020年12月经手术治疗后病理确诊的非转移性肺腺癌患者198例。根据受试者工作特征(ROC)曲线确定PdwTR的最佳截断值,并根据截断值将患者分为PdwTR低组和PdwTR高组,采用时间依赖的ROC曲线评估PdwTR、PDW、TS预测DFS的效能,Cox比例风险模型分析PdwTR与DFS的关联。结果PdwTR最佳截点值为7.45,其预测3年DFS的敏感性为93.90%,特异性为61.20%,ROC曲线下面积(AUC)为0.83(95%CI:0.75~0.90)。Log-rank检验结果显示,PdwTR低组的DFS低于PdwTR高组(log-rankχ^(2)=28.99,P<0.001);Cox回归模型调整年龄、性别、BMI、CEA水平、T分期、N分期、TNM分期、NLR、LMR和PNI等潜在混杂因素后,PdwTR高组的复发风险较PdwTR低组下降93.0%(HR=0.07,95%CI:0.02~0.33,P=0.001)。结论术前PdwTR低与非转移性肺腺癌患者DFS较差独立相关,可作为DFS的预测指标。
