In order to investigate the intratumoral DNA ploid heterogeneity (PH) in esophageal squamous cell carcinoma and its clinical-pathological significance, nuclear DNA ploidy of 80 cases of squamous cell carcinoma of the ...In order to investigate the intratumoral DNA ploid heterogeneity (PH) in esophageal squamous cell carcinoma and its clinical-pathological significance, nuclear DNA ploidy of 80 cases of squamous cell carcinoma of the esophagus were determined with multiple samples removed from the same tumor, using a flow cytometry (FCM) technique. 240 samples for flow cytimetric DNA analysis were taken from 3 different parts if each tumor of 80 cases of specimens. DNA measurement was were present in a tumor or the variation in DI value among 3 peaks in each tumor was greater than 10%. Further more at last, comparison or clinical-pathological characteristics was performed between PHTs and N-PHT which has a similar ploid pattern in 3 sampling spots of each tumor (non PHT). DNA indices ranged from 0.77～1.74, and the incidence of DNA AN was 88. 8% (71/80) in this series. Of 80 cases, 38 cases (47. 5%, 38/80) showed intratumoral heterogeneity in DNA ploidy. The heterogeneity in DNA ploidy was related to the extent of wall penetration by the tumor,the incidence of lymph node metastasis and the patients's prognosis, not to histological grades and size of the tumor. There is PH phenomena in esophageal squmous cell carcinoma, and DNA PH may be a more exact indicator in reflecting the biological chatacteristics of the tumor and patient's prognosis.展开更多
文摘In order to investigate the intratumoral DNA ploid heterogeneity (PH) in esophageal squamous cell carcinoma and its clinical-pathological significance, nuclear DNA ploidy of 80 cases of squamous cell carcinoma of the esophagus were determined with multiple samples removed from the same tumor, using a flow cytometry (FCM) technique. 240 samples for flow cytimetric DNA analysis were taken from 3 different parts if each tumor of 80 cases of specimens. DNA measurement was were present in a tumor or the variation in DI value among 3 peaks in each tumor was greater than 10%. Further more at last, comparison or clinical-pathological characteristics was performed between PHTs and N-PHT which has a similar ploid pattern in 3 sampling spots of each tumor (non PHT). DNA indices ranged from 0.77～1.74, and the incidence of DNA AN was 88. 8% (71/80) in this series. Of 80 cases, 38 cases (47. 5%, 38/80) showed intratumoral heterogeneity in DNA ploidy. The heterogeneity in DNA ploidy was related to the extent of wall penetration by the tumor,the incidence of lymph node metastasis and the patients's prognosis, not to histological grades and size of the tumor. There is PH phenomena in esophageal squmous cell carcinoma, and DNA PH may be a more exact indicator in reflecting the biological chatacteristics of the tumor and patient's prognosis.
