目的:探讨卵巢癌组织中DNA损伤激活的非编码RNA(non-coding RNA activated by DNA damage,NORAD)的表达水平及其与临床病理特征和预后的相关性。方法:选取2019年至2020年于青岛大学附属医院诊治的50例卵巢癌患者和50例正常卵巢患者的组...目的:探讨卵巢癌组织中DNA损伤激活的非编码RNA(non-coding RNA activated by DNA damage,NORAD)的表达水平及其与临床病理特征和预后的相关性。方法:选取2019年至2020年于青岛大学附属医院诊治的50例卵巢癌患者和50例正常卵巢患者的组织标本。通过实时荧光定量反转录聚合酶链式反应(qRT-PCR)方法检测以上患者组织中NORAD的相对表达水平,并分析NORAD的表达水平与临床病理特征和预后的关系。使用Kaplan-Meier plotter在线数据库评估NORAD的表达与卵巢癌预后的相关性。结果:NORAD在卵巢癌组织中呈高表达,其表达与FIGO分期、组织类型、腹水以及淋巴结转移相关(P<0.05)。NORAD高表达的卵巢癌患者5年无进展生存期(progression-free survival,PFS)显著低于低表达组。NORAD在卵巢癌组织中的表达水平在FIGO分期、病理分级、TP53状态以及化疗方面对患者的预后具有评估价值。结论:卵巢癌组织中NORAD表达水平明显升高,且其水平与卵巢癌临床病理特征及预后相关。展开更多
背景与目的:低密度脂蛋白受体相关蛋白8(low-density lipoprotein receptor-related protein 8,LRP8)在肝癌、肺癌、乳腺癌、结直肠癌等多种肿瘤发生中起重要作用,分析LRP8在胃癌中的表达及其意义,探讨LRP8作为胃癌生物治疗新靶点的可...背景与目的:低密度脂蛋白受体相关蛋白8(low-density lipoprotein receptor-related protein 8,LRP8)在肝癌、肺癌、乳腺癌、结直肠癌等多种肿瘤发生中起重要作用,分析LRP8在胃癌中的表达及其意义,探讨LRP8作为胃癌生物治疗新靶点的可能性。方法:利用人类癌症基因组图谱(The Cancer Genome Atlas,TCGA)和基因表达汇编(Gene Expression Omnibus,GEO)数据库分析LRP8在胃癌组织中的表达;对胃癌中与LRP8共表达的基因进行基因本体(GeneOntology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析;利用RNA干扰技术及细胞功能实验,测定LRP8表达对胃癌细胞系AGS增殖和迁移能力的影响,并检测LRP8敲低对Wnt信号通路活性的影响。利用Kaplan-Meier plotter数据库分析LRP8高低表达与胃癌患者预后的相关性;分析TCGA数据库中240例和郑州大学第五附属医院2018年8月—2019年7月行全胃切除术或部分切除术的25例胃癌患者LRP8表达及其与临床病理学特征之间的关系。结果:TCGA数据库中胃癌组织中LRP8表达高于正常胃黏膜,差异有统计学意义(P<0.000 1),GEO数据库中3项研究结果一致(P<0.05)。细胞功能实验表明,AGS细胞系中沉默LRP8表达后,相比对照组,si-LRP8组AGS细胞在体外增殖率和迁移率下降(P<0.05),且Wnt通路下游关键靶点β-catenin、LRP5、POU5F1、CCND1、AXIN2表达下调(P<0.05);KaplanMeier plotter数据库中两项芯片的生存分析显示,LRP8高表达患者预后不良(均P<0.05);TCGA数据库中分析显示,LRP8高表达与胃癌患者年龄呈正相关(P<0.001);回顾性分析显示LRP8高表达与胃癌分期正相关(P=0.034)。结论:LRP8在胃癌中高表达,并通过影响Wnt/β-catenin信号转导通路促进胃癌细胞恶性行为。LRP8的高表达与胃癌患者的远期生存率下降相关,有望成为胃癌患者治疗或预后的生物学标志物。展开更多
BACKGROUND Kinesin super family 23(KIF23)is a member of the KIF family,and it plays an important role in mitosis and cytokinesis.Loss of expression can cause mitotic arrest.The Oncomine database is one of the largest ...BACKGROUND Kinesin super family 23(KIF23)is a member of the KIF family,and it plays an important role in mitosis and cytokinesis.Loss of expression can cause mitotic arrest.The Oncomine database is one of the largest oncogene chip databases in the world,and is an integrated data mining platform for cancer gene information.By querying the database,differences in expression between tumor tissue and normal tissue can be determined.AIM To study the expression and prognostic significance of KIF23 in gastric cancer(GC).METHODS We used immunohistochemistry to compare the expression of KIF23 in GC and normal gastric tissues.We mined the data on the expression and prognosis of KIF23 in GC using Oncomine and Kaplan-Meier plotter database.RESULTS Compared with normal gastric tissues,KIF23 expression was increased in GC tissues,and correlated with T,N,and tumor-node-metastasis stages.Survival analysis showed that patients with high expression of KIF23 had a poor overall survival.There were five studies in the Oncomine database in which expression of KIF23 was significantly higher in GC tissues than in normal gastric tissues(P<0.05).Kaplan-Meier plotter database analysis showed that recurrence-free survival,overall survival,distant metastasis free survival,and post progression survival of patients with high expression of KIF23 were lower than those of patients with low expression.Further stratified analysis found that prognostic survival indicators worsened in patients with T2 and T3 poorly differentiated adenocarcinoma with high expression of KIF23.CONCLUSION KIF23 is highly expressed in GC and is associated with a poor prognosis of patients.It may be of great significance in the diagnosis,treatment,and prognostic evaluation of GC.展开更多
文摘目的:探讨卵巢癌组织中DNA损伤激活的非编码RNA(non-coding RNA activated by DNA damage,NORAD)的表达水平及其与临床病理特征和预后的相关性。方法:选取2019年至2020年于青岛大学附属医院诊治的50例卵巢癌患者和50例正常卵巢患者的组织标本。通过实时荧光定量反转录聚合酶链式反应(qRT-PCR)方法检测以上患者组织中NORAD的相对表达水平,并分析NORAD的表达水平与临床病理特征和预后的关系。使用Kaplan-Meier plotter在线数据库评估NORAD的表达与卵巢癌预后的相关性。结果:NORAD在卵巢癌组织中呈高表达,其表达与FIGO分期、组织类型、腹水以及淋巴结转移相关(P<0.05)。NORAD高表达的卵巢癌患者5年无进展生存期(progression-free survival,PFS)显著低于低表达组。NORAD在卵巢癌组织中的表达水平在FIGO分期、病理分级、TP53状态以及化疗方面对患者的预后具有评估价值。结论:卵巢癌组织中NORAD表达水平明显升高,且其水平与卵巢癌临床病理特征及预后相关。
文摘背景与目的:低密度脂蛋白受体相关蛋白8(low-density lipoprotein receptor-related protein 8,LRP8)在肝癌、肺癌、乳腺癌、结直肠癌等多种肿瘤发生中起重要作用,分析LRP8在胃癌中的表达及其意义,探讨LRP8作为胃癌生物治疗新靶点的可能性。方法:利用人类癌症基因组图谱(The Cancer Genome Atlas,TCGA)和基因表达汇编(Gene Expression Omnibus,GEO)数据库分析LRP8在胃癌组织中的表达;对胃癌中与LRP8共表达的基因进行基因本体(GeneOntology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析;利用RNA干扰技术及细胞功能实验,测定LRP8表达对胃癌细胞系AGS增殖和迁移能力的影响,并检测LRP8敲低对Wnt信号通路活性的影响。利用Kaplan-Meier plotter数据库分析LRP8高低表达与胃癌患者预后的相关性;分析TCGA数据库中240例和郑州大学第五附属医院2018年8月—2019年7月行全胃切除术或部分切除术的25例胃癌患者LRP8表达及其与临床病理学特征之间的关系。结果:TCGA数据库中胃癌组织中LRP8表达高于正常胃黏膜,差异有统计学意义(P<0.000 1),GEO数据库中3项研究结果一致(P<0.05)。细胞功能实验表明,AGS细胞系中沉默LRP8表达后,相比对照组,si-LRP8组AGS细胞在体外增殖率和迁移率下降(P<0.05),且Wnt通路下游关键靶点β-catenin、LRP5、POU5F1、CCND1、AXIN2表达下调(P<0.05);KaplanMeier plotter数据库中两项芯片的生存分析显示,LRP8高表达患者预后不良(均P<0.05);TCGA数据库中分析显示,LRP8高表达与胃癌患者年龄呈正相关(P<0.001);回顾性分析显示LRP8高表达与胃癌分期正相关(P=0.034)。结论:LRP8在胃癌中高表达,并通过影响Wnt/β-catenin信号转导通路促进胃癌细胞恶性行为。LRP8的高表达与胃癌患者的远期生存率下降相关,有望成为胃癌患者治疗或预后的生物学标志物。
文摘BACKGROUND Kinesin super family 23(KIF23)is a member of the KIF family,and it plays an important role in mitosis and cytokinesis.Loss of expression can cause mitotic arrest.The Oncomine database is one of the largest oncogene chip databases in the world,and is an integrated data mining platform for cancer gene information.By querying the database,differences in expression between tumor tissue and normal tissue can be determined.AIM To study the expression and prognostic significance of KIF23 in gastric cancer(GC).METHODS We used immunohistochemistry to compare the expression of KIF23 in GC and normal gastric tissues.We mined the data on the expression and prognosis of KIF23 in GC using Oncomine and Kaplan-Meier plotter database.RESULTS Compared with normal gastric tissues,KIF23 expression was increased in GC tissues,and correlated with T,N,and tumor-node-metastasis stages.Survival analysis showed that patients with high expression of KIF23 had a poor overall survival.There were five studies in the Oncomine database in which expression of KIF23 was significantly higher in GC tissues than in normal gastric tissues(P<0.05).Kaplan-Meier plotter database analysis showed that recurrence-free survival,overall survival,distant metastasis free survival,and post progression survival of patients with high expression of KIF23 were lower than those of patients with low expression.Further stratified analysis found that prognostic survival indicators worsened in patients with T2 and T3 poorly differentiated adenocarcinoma with high expression of KIF23.CONCLUSION KIF23 is highly expressed in GC and is associated with a poor prognosis of patients.It may be of great significance in the diagnosis,treatment,and prognostic evaluation of GC.
