[Objective] The aim was to explore the molecular mechanisms of the A223C polymorphism at 3＇UTR of oxidized low density lipoprotein receptor 1（OLR1）which was closely related to milk fat percentage in cow.[Method]The...[Objective] The aim was to explore the molecular mechanisms of the A223C polymorphism at 3＇UTR of oxidized low density lipoprotein receptor 1（OLR1）which was closely related to milk fat percentage in cow.[Method]The putative microRNA targets located in the 3＇UTR of OLR1 were predicted with the software miRanda（1.0）which could carry the microRNA database of cow.[Result]Total 16 microRNA targets were found.The A223C SNP was located in complementary region of bta-miR-370 target sequence.[Conclusion]OLR1 3＇UTR A→C mutation had led to the disappearing of bta-miR-370 target,which had provided basis for the further research on the molecular mechanisms of the A223C polymorphism at 3＇UTR of OLR1 gene which was closely related to milk fat percentage in cow.展开更多
In this study, the genes encoding main structural proteins and 3′ untranslated region (UTR) of a commercial domestic vaccine strain H52 and a commercial foreign vaccine strain H52 of infectious bronchitis virus (IBV)...In this study, the genes encoding main structural proteins and 3′ untranslated region (UTR) of a commercial domestic vaccine strain H52 and a commercial foreign vaccine strain H52 of infectious bronchitis virus (IBV) were obtained by RT-PCR, then were cloned and sequenced respectively. The sequence analyses showed the genetic information of S1 gene, M gene, N gene and 3′UTR of domestic vaccine strain H52 was remarkably different from that of foreign H52 strain and that of published sequences of H52 strain. Compared with foreign H52 strain, this domestic H52 strain was genetically closer with M41 strain and was located in the same branch of phylogenetic tree based on the sequences of main structural protein genes. The results strongly suggested that it was necessary to build genetic information archives of seed stock of IBV vaccine strains.展开更多
基金Supported by Key Project in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period(No.2008BADB2B04-8-4)Natural Science Foundation of Hebei Province(No.C2008001308 and C2007000743)~~
文摘[Objective] The aim was to explore the molecular mechanisms of the A223C polymorphism at 3＇UTR of oxidized low density lipoprotein receptor 1（OLR1）which was closely related to milk fat percentage in cow.[Method]The putative microRNA targets located in the 3＇UTR of OLR1 were predicted with the software miRanda（1.0）which could carry the microRNA database of cow.[Result]Total 16 microRNA targets were found.The A223C SNP was located in complementary region of bta-miR-370 target sequence.[Conclusion]OLR1 3＇UTR A→C mutation had led to the disappearing of bta-miR-370 target,which had provided basis for the further research on the molecular mechanisms of the A223C polymorphism at 3＇UTR of OLR1 gene which was closely related to milk fat percentage in cow.
文摘In this study, the genes encoding main structural proteins and 3′ untranslated region (UTR) of a commercial domestic vaccine strain H52 and a commercial foreign vaccine strain H52 of infectious bronchitis virus (IBV) were obtained by RT-PCR, then were cloned and sequenced respectively. The sequence analyses showed the genetic information of S1 gene, M gene, N gene and 3′UTR of domestic vaccine strain H52 was remarkably different from that of foreign H52 strain and that of published sequences of H52 strain. Compared with foreign H52 strain, this domestic H52 strain was genetically closer with M41 strain and was located in the same branch of phylogenetic tree based on the sequences of main structural protein genes. The results strongly suggested that it was necessary to build genetic information archives of seed stock of IBV vaccine strains.
