Electroacupuncture at Dazhui(GV14) and Mingmen(GV4) protects against spinal cord injury:the role of the Wnt/β-catenin signaling pathway

Electroacupuncture at Dazhui（GV14） and Mingmen（GV4） on the Governor Vessel has been shown to exhibit curative effects on spinal cord injury; however, the underlying mechanism remains poorly understood. In this study, we established rat models of spinal cord injury using a modified Allen＇s weight-drop method. Ninety-nine male Sprague-Dawley rats were randomly divided into three equal groups： sham（only laminectomy）, SCI（induction of spinal cord injury at T10）, and EA（induction of spinal cord injury at T10 and electroacupuncture intervention at GV14 and GV4 for 20 minutes once a day）. Rats in the SCI and EA groups were further randomly divided into the following subgroups： 1-day（n = 11）, 7-day（n = 11）, and 14-day（n = 11）. At 1, 7, and 14 days after electroacupuncture treatment, the Basso, Beattie and Bresnahan locomotor rating scale showed obvious improvement in rat hind limb locomotor function, hematoxylin-eosin staining showed that the histological change of injured spinal cord tissue was obviously alleviated, and immunohistochemistry and western blot analysis showed that Wnt1, Wnt3 a, β-catenin immunoreactivity and protein expression in the injured spinal cord tissue were greatly increased compared with the sham and SCI groups. These findings suggest that electroacupuncture at GV14 and GV4 upregulates Wnt1, Wnt3 a, and β-catenin expression in the Wnt/β-catenin signaling pathway, exhibiting neuroprotective effects against spinal cord injury.

COMPREHENSIVE EVALUATION ON ACPUNCTURE TREATMENT OF CLINICAL SYMPTOMS AND SIGNS WITH DZHUī (大椎GV14) IN COMMON COLD PATIENTS WITH HIGH FEVER

Objective To probe into the effective acupuncture treatment for high fever and other clinical relevant symptoms in common cold. Methods Based on randoming and multi-central clinical trial principle, the included 276 cases were randomized into experimental group （138 cases） and control group （138 cases） in statistics. Electric acupuncture on Dazhui（大椎 GV14） was applied in experimental group and the injection with antondine was in control group. The immediate antipyretic and clinical therapeutic effects were observed in 24 h. Results The body temperatures at different times in experimental group were all lower than those in control group after treatment （P〈 0.01 ）. The scores of relevant symptoms in 3- 24 h after treatment were lower than those in control group （P〈0.05）. The response time （1.42± 1.79） h in experimental group was shorter than that （3.44±5.10） h in control group （P〈0.05）. The response times for chills, soreness of limbs, headach and sweating in experimental group were shorter than those in control group （P〈0. 01 ）. The recovery times for chills and headache in experimental group were shorter than those in control group （P〈0.05）. The systematic therapeutic effects were different significantly between two groups in 24 h （P〈0.05）. Conclusion Acupuncture on Dazhui（大椎 GV14） had achieved definite effects on treatment for high fever in common cold. It released remarkably chills, soreness of limbs, headache and sweating and improved the systematic therapeutic effects on common cold.

ACUPUNCTURE TREATMENT OF OCCIPITAL NEURALGIA WITH DAZHUI AND FENGCHI ACUPOINTS

Objective: To explore therapeutic method for occipital neuralgia. Methods: A total of 90 occipital neuralgia patients were randomly divided into treatment group (n=60 cases) and control group (n=30). In treatment group, Dazhui (GV 14) was punctured and stimulated with reinforcing method, and Fengchi (GB 20) punctured and stimulated with reducing needling method. In control group, the tender point was punctured and stimulated with reducing needling method. The acupuncture needles were retained for 30 min and the treatment was conducted once daily, with 10 times being a therapeutic course. Results: After one session of treatment, the markedly effective rates of treatment and control groups were 98% and 57% respectively. Comparison between two groups showed a considerable difference in the therapeutic effect (P<0.05). Conclusion: Acupuncture of Dazhui (GV 14) and Fengchi (GB 20) are effective in the treatment of occipital neuralgia.

Effect of stimulating the acupoints Feishu (BL 13) and Dazhui (GV14) on transdermal uptake of sinapine thiocyanate in asthma gel

OBJECTIVE: To investigate the effect of stimulating the acupoints Feishu(BL 13) and Dazhui(GV 14) on the transdermal uptake of sinapine thiocyanate contained in a gel used for the management of asthma.METHODS: Thirty Sprague-Dawley rats were randomly divided into three equal groups using a random number table: the Feishu(BL 13) acupoint group, the Dazhui(GV 14) acupoint group, and the nonacupoint group or control group. Using micro-dialysis technology, preprocessed skin probes were implanted into the rats at Feishu(BL 13), Dazhui(GV 14), and a nonacupoint site. Asthma gel was then placed on the skin at Feishu(BL 13), Dazhui(GV14) acupoints, and the nonacupoint for all groups.Dialysate was collected every 30 min for 12 h. The normalized concentration of sinapine thiocyanate in the skin was determined by high-performance liquid chromatography.RESULTS: The rat in vivo transdermal experiment demonstrated that the quantity-time equation showed a good linear correlation with zero-order kinetics(r > 0.99). The transdermal behavior was in accordance with the first-order rate open model in which the transdermal penetration rates and the accumulative amounts of sinapine thiocyanate in the skin at the acupoint sites were greater than those through the skin of the nonacupoint site. The systemic maximum concentration and the area under the curve of sinapine thiocyanate in the acupoint groups were significantly greater than in the nonacupoint group. A lag time was observed in both acupoint groups, but not in the nonacupoint group.CONCLUSION: Stimulating the acupoints promotes the percutaneous absorption of sinapine thiocyanate and also controls its release, reducing concentration fluctuations in the blood.

Catgut implantation at acupoints increases the expression of glutamate aspartate transporter and glial glutamate transporter-1 in the brain of rats with spasticity after stroke

Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui（GV14）,Jizhong（GV6）,Houhui,Guanyuan（CV4）and Zhongwan（CV12）.The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter（GLAST）and glial glutamate transporter-1（GLT-1）expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1m RNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 m RNAs was increased on the injured（left）side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.

The influential point of bone should be Dàzhuī(大椎 GV 14) rather than Dàzhù(大杼 BL 11)

Objective The influential point of bone in acupuncture should be Dàzhuī（大椎 GV 14）, but it is wrongly believed as Dàzhù（大杼 BL 11） in the modern acupuncture literature and textbooks. This wrong academic theory has widely influenced acupuncture field domestically and abroad, and misguided acupuncture medicine theory and the treatment of acupuncture on bone diseases. This essay aims to clear up the misunderstanding and restore the academic status of ＂the influential point of bone is GV 14＂ in the acupuncture theory and the treatment on bone diseases.

针挑治疗颈椎病的随机对照研究

目的:运用随机对照临床研究方法,对针挑治疗颈椎病进行疗效评价。方法:将158例患者随机分为针挑治疗组(针挑组)56例、常规针刺对照组(针刺组)55例与局麻对照组(局麻组)47例。取穴均为颈百劳、大椎、肩井等,分别采用针挑法、常规针刺法及局部麻醉法治疗。采用简化McGill疼痛询问量表等量化计分,结合临床症状、体征进行疗效分析。结果:针挑组痊愈率为57.1%,优于针刺组的23.6%与局麻组的14.9%(均P<0.01),针挑治疗基本无不良反应。结论:针挑疗法对于颈椎病是一种高效、安全的疗法。

大椎、关元对腰椎间盘突出症临床疗效的影响

目的:探讨大椎、关元对腰椎间盘突出症临床疗效的影响,以及任督脉在腰椎间盘突出症临床治疗上的指导意义。方法:将427例腰椎间盘突出症患者随机单盲分为常规(循经取穴)组、常规加大椎组和常规加关元组进行治疗观察,采用u检验评定比较各组间疗效。结果:加大椎组和加关元组的治愈率与常规组比较,差异有显著或非常显著性意义(P<0.05,P<0.01),而有效率组间比较差异无显著性意义(P>0.05)。结论:大椎、关元可以提高腰椎间盘突出症的治愈率,调节任督、温补阳气可能是治疗腰椎间盘突出症的重要手段和方法之一。

针刺大椎治疗感冒高热临床观察

目的:观察针刺退热的临床疗效。方法:将86例高热患者随机分为两组,针刺组45例,针刺大椎;药物组41例,肌肉注射安痛定注射液。观察治疗前后24小时内患者的相关症状的改善和体温变化,采用单盲、前后对照的方法。结果:针刺组痊愈9例,药物组痊愈2例。针刺组退热效果明显优于药物组。结论:针刺退热疗效可靠,值得临床推广应用。

电针“大椎”“命门”对佐剂性关节炎大鼠应激相关因子影响的实验研究

目的:通过观察电针“大椎”“命门”穴后下丘脑CRH、血浆ACTH、血清Cort、TNF-α含量的关系,分析电针不同穴位对应激相关因子的影响。方法:以佐剂性关节炎大鼠(AA)作为研究对象,随机分正常组、模型组、电针“大椎”组、电针“命门”组。观察电针对关节炎症局部及下丘脑CRH、血浆ACTH、血清Cort、TNF-α含量的影响。结果:“大椎”组、“命门”组经治疗右后足足爪肿胀率与模型对照组比较极明显降低(P<0.01),“大椎”组下丘脑CRH含量、血清Cort、TNF-α含量,“命门”组血清Cort含量与模型对照组比较明显降低(P<0.05)。结论:电针具有一定的抗炎作用,并且这种作用可能是通过对CRH、Cort等应激相关因子的调节,减轻疾病应激程度而实现的。

不同时段艾灸血清中内生性蛋白组分双向电泳的实验研究

目的:从血清蛋白质组初步探讨艾灸免疫调节中蛋白质分子的变化规律。方法:采用超速离心除盐方法预处理血清,进行一维和二维聚丙烯酰胺凝胶电泳(2-DE)分离,凝胶银染显示各组蛋白质差异表达。结果:2-DE图谱中可辨识的蛋白质斑点达300～400个,除有看家蛋白、特异蛋白外,不同时段存在差异表达蛋白,从十几个至几十个不等。艾灸经穴组的蛋白质组分变化较为明显,以酸性蛋白点缺失和蛋白峰度下降最为主,而非经穴组血清蛋白变化不明显。结论:在特定穴位上艾灸刺激有助于清除血清中的免疫抑制物,使艾灸血清的蛋白质组分发生以酸性蛋白缺失为主的改变。

针刺百会、大椎对老年性痴呆大鼠脑内SOD及AChE的影响

目的探讨针刺百会、大椎对老年性痴呆大鼠的脑内超氧化物歧化酶(SOD)及乙酰胆碱酯酶(AChE)含量的影响。方法选用纯系Wistar老年大鼠52只,随机分为假手术组、模型组、针刺组和西药组,采用β-淀粉样蛋白向海马CA1区定向注射制作老年性痴呆模型,进行百会、大椎针刺治疗。检测大鼠脑内SOD及AChE的含量。结果针刺老年性痴呆大鼠的百会、大椎穴,可明显提高脑组织内SOD的含量,降低脑组织内AChE的含量,与模型组比较有统计学意义(P<0.05)。结论针刺能提高老年性痴呆大鼠的学习记忆能力,改善自由基代谢,促进老年性痴呆大鼠的智能恢复。

隔姜温针灸治疗持续性变应性鼻炎的随机对照试验

目的观察隔姜温针灸大椎穴治疗持续性变应性鼻炎的临床疗效。方法采用前瞻性随机平行对照设计,将60例持续性变应性鼻炎患者随机分为隔姜温针灸组、单纯针刺组、药物对照组各20例。2个针刺组选穴相同,区别为是否于大椎穴隔姜温针灸,药物组口服氯雷他定片。3组均治疗4周,并于治疗前后评估疗效,6个月后随访,2个针刺组另于首次和末次治疗后的1、8、24、48 h共8个时点进行TNSS+TNNSS量表自评。结果 3组治疗后症状计分、TNSS+TNNSS评分和RQLQ评分均较同组治疗前明显降低(P<0.01),但3组组间比较差异均无统计学意义(P>0.05)。隔姜温针灸组和单纯针刺组在8个时点的TNSS+TNNSS量表得分均较同组治疗前明显下降(P<0.01),2组相同时点的组间比较差异无统计学意义(P>0.05)。单纯针刺组末次治疗后,第8、24、48 h的得分较第1h均有增加(P<0.05);隔姜温针灸组末次疗后四时点得分差异无统计学意义(P>0.05)。单项症状分数比较,隔姜温针灸组疗后鼻塞症状得分明显低于药物对照组(P<0.05),单纯针刺组疗后眼部症状得分明显高于药物对照组(P<0.05)。3组6个月后症状计分和生活质量得分均较同组治疗前明显降低(P<0.05),且隔姜温针灸组6个月后症状计分明显低于单纯针刺组(P<0.05)。结论隔姜温针灸疗法比单纯针刺疗法有更长的疗效持续时间和更好的远期疗效,且该疗法对鼻塞的疗效优于氯雷他定。

青鹏膏剂穴位贴敷对肥大细胞数量及脱颗粒的影响

目的:观察体表穴区贴敷青鹏膏剂后,大鼠穴区内肥大细胞(MCs)的分布以及数量和脱颗粒的变化,了解穴位贴敷对皮肤组织的影响,研究其发挥作用的可能性机理。方法:健康Wistar大鼠30只,随机分为空白对照组(A组)、电针组(B组)和青鹏膏剂贴敷组(C组),每组10只。干预组动物给予青鹏膏剂贴敷或电针刺激7 d后,分别取3组动物的"大椎"穴区皮肤组织块做冰冻切片,经亚铁氯化铜法示乙酰胆碱酯酶并甲苯胺兰复染,比较3组"大椎"穴区组织MCs分布、数量及脱颗粒的差异,研究穴区内MCs的形态学改变。结果:各组"大椎"穴区真皮、皮下组织和肌肉组织中都分布着大小、数量不等的MCs,以皮下组织层分布最为广泛,多沿神经或血管分布。与A组比较,青鹏膏剂贴敷组穴区组织局部MCs数量及脱颗粒率显著升高,差异有显著性(P<0.05)。结论:青鹏膏剂穴位贴敷能够明显引起皮肤组织内MCs数量增多及脱颗粒,有促聚集作用,是穴位贴敷与经络、药物协同增效的可能性因素之一。

大椎风池通治内外风证探讨

通过内、外风皆有风证共性和大椎、风池两穴所在经脉以及典型病例的治疗，认为大椎风池可以作为通治内外诸风证之主穴。通过手法可体现穴位的双向调节功能。根据病情配穴可使针感直达病所以提高和加速疗效。

艾灸大椎、阳陵泉速降血沉211例临床观察

艾灸大椎、阳陵泉速降血沉２１１例临床观察吴炳煌，林燕（福建中医学院针推系，福州３５０００３；福建省人民医院）主题词血沉／针灸效应，穴．大椎，穴．阳陵泉，艾条灸，风湿病／针灸疗法ＣｌｉｎｉｃａｌＯｂｓｅｒｖａｔｉｏｎｏｎ２１１ＣａｓｅｓＴｒｅａｔｅｄｂ...

Three heavy manipulations therapy at Dàzhuī (大椎 GV 14) for difficult diseases

With introduction of three heavy manipulations therapy at Dazhui （大椎 GV 14） for unclear enunciation case of acute cerebral infarction, acute attack case of chronic asthmatic bronchitis, amyotrophic lateral sclerosis case of motor neuron disease, ankylosing spondylitis case and chronic fatigue syndrome case, it is confirmed thatDazhui （大椎 GV 14） combined with three heavy manipulations therapy of blood-letting acupuncture, cupping and moxibust-ion has efficacy for external and endogenous wind, promoting and tonifying yang- qi, strengthening the body timely, removing wind and dampness, and relieving the pain and spasm in the clinical practice.

针刺大椎对感冒高热退热效果的临床观察

目的:探讨大椎穴对感冒高热的治疗作用。方法:将261例患者随机分为治疗组(133例)和对照组(128例)。治疗组采用电针大椎,对照组采用肌肉注射安痛定注射液,观察24小时的即时退热效果。结果:治疗组治疗后各时点体温均低于对照组(P<0.01)。治疗组起效时间为(1.42±1.79)小时,短于对照组的(3.44±5.10)小时(P<0.01)。治疗组痊愈率为27.8%,解热率为75.9%;对照组痊愈率为10.9%,解热率为55.5%,两组比较差异均有非常显著性意义(P<0.01)。风热证感冒治疗组的解热率为75.3%,对照组为50.0%,两组比较差异有非常显著性意义(P<0.01)。结论:大椎穴退热疗效确切,风热感冒所致高热可首选大椎,治疗应越早越好。

大椎穴点刺加拔罐治疗颅内高压综合征40例

目的:观察大椎穴点刺加拔罐对静脉点滴甘露醇患者脑脊液压力的影响。方法:80例患者随机分为治疗组和对照组。治疗组用静脉点滴甘露醇加大椎穴点刺拔罐治疗,对照组静脉点滴甘露醇治疗。结果:治疗组显效率(45.0％)明显高于对照组(17.5％),总有效率治疗组(82.8％)也明显高于对照组(60.0％),两组之间比较差异有非常显著性意义(P<0.01)。结论:大椎穴点刺加拔罐配合治疗对颅内高压综合征有良好的效果。

穴位敷贴治疗梅核气(肝郁气滞型)30例

目的观察中药穴位敷贴治疗梅核气(肝郁气滞型)的临床疗效。方法对30例梅核气(肝郁气滞型)患者,选取疏肝理气、散结解郁的中药按比例研末,并以适量蜂蜜或醋调匀,制成直径约2 cm,厚度约0.3cm的药膏,用胶布将块状药膏贴于大椎、天突、膻中穴位上进行治疗。结果治愈17例,有效9例,无效4例,总有效率86.67%。结论中药穴位敷贴治疗梅核气(肝郁气滞型),可疏通经络,调节人体内分泌功能,疏肝理气、散结解郁,调节全身气血阴阳,明显改善临床症状。