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Mutations in the p16 gene in DMBA-induced pancreatic intraepithelial neoplasia and pancreatic cancer in rats 被引量:2
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作者 Zhu Zhu Tao Liu +2 位作者 Fei Han Su-Dong Zhan Chun-You Wang 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS CSCD 2015年第2期208-214,共7页
BACKGROUND:7,12-dimethylbenzanthracene(DMBA)-induced pancreatic intraepithelial neoplasia(PanIN)and pancreatic cancer in rats provide a classic model for uncovering the molecular mechanisms underlying pancreatic ... BACKGROUND:7,12-dimethylbenzanthracene(DMBA)-induced pancreatic intraepithelial neoplasia(PanIN)and pancreatic cancer in rats provide a classic model for uncovering the molecular mechanisms underlying pancreatic cancer.However,this model has not been characterized genetically,and in particular,the major genetic alterations in the p16 gene are unknown.METHODS: Lesions of PanlN and pancreatic cancer were induced with DMBA implantation in 40 rats, and control pancreatic tissue was obtained from 10 age-matched rats without exposure to DMBA. Pancreatic tissue was harvested three months after DMBA implantation and DNA was extracted. Homozy- gous deletions and point mutations of the pl6 (exons 1 and 2) gene were detected by PCR amplification and direct sequencing. RESULTS: DMBA implantation in the 40 rats induced 26 Pan- INs and 9 carcinomas. The overall frequency of p 16 alterations in the pancreatic tissue of these rats was 42.86% (15/35), and the changes were point mutations, not homozygous deletions. p16 mutations were present in 30.77% (8/26) of the rats with PanIN and 77.78% (7/9) of the rats with carcinoma (P〈0.05). The increasing incidence of p16 alterations was detected in 20.00% (1/5) of PanIN-1, 28.57% (2/7) of PanIN-2 and 35.71% (5/14) of PanIN-3 lesions. CONCLUSION: Our findings indicated that p16 alteration is a common event in the carcinogenesis of this model and that the mutation pattern is analogous to that of human lesions. 展开更多
关键词 homozygous deletion point mutation p16 pancreatic intraepithelial neoplasia pancreatic carcinoma
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Systematic examination of DNA variants in the parkin gene in patients with Parkinson’s disease 被引量:2
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作者 王涛 梁直厚 +4 位作者 孙圣刚 曹学兵 彭海 刘红进 童萼塘 《Chinese Medical Journal》 SCIE CAS CSCD 2004年第10期1567-1569,共3页
关键词 Parkinson’s disease · sequence deletion · point mutation · Polymorphism
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