Effect of acupuncture at acupoint of Shenshu (BL 23) on the bones of ovariectomized rats

OBJECTIVE： To investigate the effect of acupuncture at acupoint of Shenshu （BL 23） in ovariectomized rats and sham-operated rats. METHODS： Female Sprague-Dawley rats were divided into the following four groups （n = 12 each）： sham operation only （Sham）, sham operation with acupuncture （Sham ＋ ACP）, ovariectomy only （OVX）, and ovariectomy with acupuncture （OVX ＋ ACP）. Operations were performed at the 9th week of age. Acupuncture of Shenshu （BL 23） was started at the 1 lth week of age and conducted 3 times per week until the 26th week of age. RESULTS： Among ovariectomized rats, the acupuncture-treated rats had significantly lower body weights and cross-linked N-telopeptide of type 1 collagen levels in the urine after 3 weeks of acu- puncture treatment, higher bone mineral density of the lumbar vertebra after 7 weeks, and smaller trabecular separation of the sixth lumbar vertebra, measured by micro-computed tomography, after 17 weeks than control rats. These effects were not observed in sham-operated rats. Blood concentrations of estradiol were significantly higher in the OVX ＋ ACP group than in the untreated group. Acupuncture treatment increased plasma testosterone levels in sham-operated rats, while this increase was not observed in OVX rats. CONCLUSION： Acupuncture of Shenshu （BL 23） prevents bone loss and structural changes by sup- pressing bone resorption.

CLINICAL APPLICATION OF “LUMBAR THREE POINT NEEDLING” IN TREATMENT OF LUMBAR AND BUTTOCK DISEASES

Lumbar three point needling”, meaning puncturing Shenshu (BL 23), Dachangshu (BL 25) and Ciliao(BL 32) at the same time, is a commonly used acupuncture method in the treatment of lumbocrural diseases. Points supplemented according to symptoms: Weizhong(BL 40) is supplemented for lumbar vertebra hypertrophy spondylitis; Weizhong (BL 40) and Kunlun(BL 60) supplemented for acute lumbar muscle sprain; Zhibian (BL 54), Huantiao (GB 30) and Ahshi points supplemented for nervi cluniam superior inflammation, and Sanyinjiao (SP 6) and Mingmen(GV 4) supplemented for impotence. Gauge 28 2 cun long needles are used. The needles are inserted perpendicularly into Shenshu(BL 23), Dachengshu(BL 25) and Ciliao(BL 32) to a depth of about 1.2～1.5 cun. Other supplementary points are needled and stimulated with routine manipulations, generally the reducing method is used for excess syndrome, and the reinforcing method applied to deficiency syndrome. The author, on the basis of many years of clinical practice, considers that “lumbar three point needling” is fairly effective in treating lumbosacral diseases.

Study of the Specificity of Lumbosacral Points in Regulating Unstable Bladder

Objective： To investigate the effects of acupuncture of Shenshu （BL 23）, Huiyang （BL 35） and Shenshu （BL 23） plus Huiyang （BL 35） on the main symptoms of unstable bladder （USB） and explore the specificity of different points in treating USB through a clinical randomized controlled trial. Methods： Eighty-nine USB patients were allocated to three groups： Shenshu （BL 23）, Huiyang （BL 35） and Shenshu （BL 23） plus Huiyang （BL 35）. I-PSS was used as an observation index. The effects were evaluated and a comparison was made between the groups after three and six treatments. Results： I-PSS significantly improved in all Shenshu （BL 23）, Huiyang （BL 35） and Shenshu （BL 23） plus Huiyang （BL 35） groups of USB patients （P〈0.05）. The effect was significantly better in the Huiyang （BL 35） and Shenshu （BL 23） plus Huiyang （BL 35） groups than in the Shenshu （BL 23） group （P〈0.05）. The efficacy rate was 50.0% in the Shenshu （BL 23） group, 82.8% in the Huiyang （BL 35） group and 90.0% in the Shenshu （BL 23） plus Huiyang （BL 35） group. It was significantly higher in the Huiyang （BL 35） and Shenshu （BL 23） plus Huiyang （BL 35） groups than in the Shenshu （BL 23） group （P〈0.05）. Conclusion： Points Shenshu （BL 23）, Huiyang （BL 35） and Shenshu （BL 23） plus Huiyang （BL 35） can all regulate voiding dysfunction and improve quality of life in USB patients. Shenshu （BL 23） plus Huiyang （BL 35） has the most marked therapeutic effect.

Acupoints selection pattern in acupuncture-moxibustion treatment of perimenopausal syndrome from 2007 to 2016

Objective： To discuss the acupoints selection pattern in acupuncture-moxibustion treatment of perimenopausal syndrome（PMS） from 2007 to 2016. Methods： Clinical literatures related to PMS treated with acupuncture-moxibustion published from 2007 to 2016 were collected from Chinese Biomedical Literature Database（CBM）, Chongqing VIP Database（CQVIP）, China National Knowledge Infrastructure（CNKI）, and Wanfang Academic Journal Full-text Database（Wanfang）. The retrieved data underwent descriptive analysis, cluster analysis and association pattern analysis to determine the acupoints selection principle in acupuncture-moxibustion treatment of PMS. Results： The top five acupoints used in acupuncture-moxibustion treatment of PMS were Sanyinjiao（SP 6）, Shenshu（BL 23）, Guanyuan（CV 4）, Baihui（GV 20）, and Shenmen（HT 7）. The leading 4 meridians were Bladder Meridian, Conception Vessel, Spleen Meridian, and Governor Vessel. The clustering analysis showed that the 5 core acupoint groups were：（1） Sanyinjiao（SP 6）;（2） Shenshu（BL 23） and Guanyuan（CV 4）;（3） Baihui（GV 20）, Shenmen（HT 7）, Zusanli（ST 36）, Ganshu（BL 18） and Taichong（LR 3）;（4） Taixi（KI 3）, Pishu（BL 20）, Xinshu（BL 15）, Qihai（CV 6） and Neiguan（PC 6）;（5） Sishencong（EX-HN 1）, Zhongwan（CV 12）, Hegu（LI 4）, Yintang（GV 29）, Fengchi（GB 20）, Zhongji（CV 3） and Feishu（BL 13）. The three most significant acupoints were Sanyinjiao（SP 6）, Shenshu（BL 23） and Guanyuan（CV 4）. Acupoint groups based on syndrome differentiation included：（1） Hegu（LI 4）, Zhongwan（CV 12） and Sishencong（EX-HN 1）;（2） Feishu（BL 13）, Zhongji（CV 3）, Fengchi（GB 20） and Yintang（GV 29）;（3） Xinshu（BL 15）, Pishu（BL 20）, Qihai（CV 6）, Neiguan（PC 6） and Taixi（KI 3）;（4） Ganshu（BL 18）, Zusanli（ST 36）, Shenmen（HT 7）, Taichong（LR 3） and Baihui（GV 20）. The analysis of association pattern elaborated that Shenshu（BL 23） and Sanyinjiao（SP 6） won the highest support rate in the paired groups; Ganshu（BL 18）, Shenshu（BL 23） and Sanyinjiao（SP 6） had the highest support rate among the acupoint groups. Conclusion： The data mining results of acupuncture-moxibustion treatment of PMS substantially conform to the general principle in traditional acupuncture-moxibustion theories, able to reflect the acupoints selection and grouping pattern and provide references for acupuncture-moxibustion treatment of PMS.

Clinical observation on acupoint injection for back pain in patients with primary osteoporosis

Objective:To observe the clinical efficacy of acupoint injection with salmon calcitonin for back pain in elderly patients with primary osteoporosis.Methods:A total of 76 patients were selected and randomly divided into two groups by the random number table method,with 39 cases in the treatment group and 37 cases in the control group,respectively.Patients in both groups received routine anti-osteoporosis treatment.Patients in the treatment group received additional acupoint injection with salmon calcitonin at bilateral Pishu(BL 20)and Shenshu(BL 23),while patients in the control group received additional intramuscular injection with salmon calcitonin.The treatments for both groups were given once a day and lasted for 4 weeks.Visual analog scale(VAS)and Chinese Oswestry disability index(CODI)scores were observed before treatment,after 2 weeks and 4 weeks of treatment,and the use of analgesics during the treatment were recorded.Results:After treatment,the clinical efficacy in the treatment group was more significant than that in the control group(P<0.05).After 2 weeks and 4 weeks of treatment,the VAS scores in both groups showed significant intra-group and between-group differences(all P<0.05),and the CODI scores in both groups showed significant intra-group differences(all P<0.05).After 2 weeks of treatment,the CODI score showed no significant between-group difference(P>0.05).After 4 weeks of treatment,the improvement of CODI score in the treatment group was more significant than that in the control group(P<0.05).During the treatment,2 cases in the treatment group took analgesics versus 8 cases in the control group,and the result showed a significant between-group difference(P<0.05).Conclusion:For back pain in elderly patients with primary osteoporosis,based on the routine treatment of oral medication,the clinical efficacy of acupoint injection with salmon calcitonin at bilateral Pishu(BL 20)and Shenshu(BL 23)is more significant than that of intramuscular injection.Acupoint injection treatment can improve patients'conditions and reduce the use of analgesics.

Study on the differences between moxibustion at different points in the effects on the anti-fatigue ability of rats undergoing one-time exhaustive swimming

Objective:To observe the effects of moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)on the energy metabolism and endocrine metabolism indicators of rats undergoing one-time exhaustive swimming,and to explore the differences between moxibustion at different points in the effects on anti-exercise fatigue.Methods:Forty-eight male SPF rats were randomly divided into a blank group,a model group,a non-meridian and non-acupoint group,a Shenshu(BL 23)group,a Zusanli(ST 36)group,and a Shenque(CV 8)group using random number table method,with eight rats in each group.Except for the blank group,rats in the other groups were subjected to replicating the one-time exhaustive model using the weight-bearing swimming experiment.Except for the model group,the other model rats received mild moxibustion immediately after swimming.Rats in the non-meridian and non-acupoint group received mild moxibustion at bilateral subcostal non-meridian and non-acupoint points,those in the Shenshu(BL 23)group received mild moxibustion at bilateral Shenshu(BL 23),those in the Zusanli(ST 36)group received mild moxibustion at bilateral Zusanli(ST 36),and those in the Shenque(CV 8)group received mild moxibustion at Shenque(CV 8)for 15 min.Four hours after the exhaustive swimming,femoral artery blood was collected to detect blood lactate(BLA),lactate dehydrogenase(LDH),creatine kinase(CK),creatinine(CRE),blood urea nitrogen(BUN),cortisol(C)and testosterone(T)levels,and calculate the T/C ratio.Results:Compared with the blank group,rat's serum levels of BLA,LDH,CK,BUN and C in the model group and the non-meridian and non-acupoint group were increased,and serum levels of CRE and T,and T/C ratios were decreased(P<0.01 or P<0.05);compared with the model group and the non-meridian and non-acupoint group,the serum levels of BLA,LDH,CK,BUN and C in the Shenshu(BL 23)group,Zusanli(ST 36)group and Shenque(CV 8)group were decreased,and the serum CRE and T levels,and the T/C ratios were increased(all P<0.01);compared with the Shenshu(BL 23)group,the serum CK level was decreased in the Shenque(CV 8)group(P<0.01),the serum levels of T and C were decreased in the Zusanli(ST 36)group and Shenque(CV 8)group(P<0.01 or P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.01);compared with the Zusanli(ST 36)group,the serum CK and BUN levels were decreased(P<0.01,P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.05).Conclusion:Moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)shows different anti-fatigue effects by regulating the energy metabolism and endocrine metabolism in rats undergoing one-time exhaustive swimming.Moxibustion at Shenshu(BL 23)is better in promoting energy synthesis.Moxibustion at Shenque(CV 8)is more effective in regulating synthesis and decomposition of the skeletal muscle proteins.

Effect of electroacupuncture at Ganshu(BL 18) and Shenshu(BL 23) on the expression of EphB2 protein in cortex around cerebral infracted area of rat

Objective： To observe the effect of electroacupuncture （EA） on the expression of erythropoie-tin-producing hepatocyte receptor B2 （EphB2） in the cortex around the infracted area of middle cerebral artery occlusion （MCAO） rats at different timing, and to reveal the possible mechanism of acupuncture in the treatment of cerebral ischemia. Methods： A total of 180 male Sprague-Dawley （SD） rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, with 45 rats in each group. Rats in each group were further divided into three subgroups： postoperative 3 d, postoperative 14 d and postoperative 2l d groups, with 15 rats in each subgroup. The MCAO model was made by the modified occlusion method. The neurological function score, 2,3,5-triphenyl tetrazolium chloride （TFC） staining, immunohistochemistry assay, immunofluorescence double labeling method and Western blot were used to detect the corresponding indicators. Results： The neurological impairment of rats was most obvious at postoperative 3 d, and then gradually improved with time, which was more significant in the acupoint group （P〈0.05）. The change of infarcted volume was consistent with the neurological function impairment. The number of EphB2 positive cells （EphB2＋） around the infarcted area was decreased significantly at postoperative 3 d, and then gradually improved with time, which returned to the same level as that in the sham operation group at postoperative 21 d. The increase was most significant in the acupoint group （P〈0.05）, and the positive cell number was higher than that in the sham operation group （P〈0.01）. Western blot and immunohistochemistry results were basically consistent. Immunofluorescence displayed that EphB2＋ and postsynaptic density-95 positive （PSD-95＋） were co-expressed, after the MCAO operation, in the cortical neuron around the infracted area, and the number of co-expressing cells was increased gradually with time, which was most significant in the acupoint group （P〈0.05）. Conclusion： Electroacupuncture at Ganshu （BL 18） and Shenshu （BL 23） can significantly improve the neurological function and cerebral infarcted volume ratio of MCAO rats, which may be related to the activation of EphB2 expression in cortex around the infracted area and the promotion of synaptic remodeling.

Effect of moxibustion at Shenshu(BL 23) on the ethology,corticosterone and glucocorticoid receptor in aging rats

Objective：To observe the effect of moxibustion on learning and memory abilities,corticosterone and glucocorticoid receptor（GR） in subacute aging rats.Methods：Twenty four Sprague-Dawley（SD） rats were randomly divided into a normal group,a model group and a moxibustion group,8 rats in each group.Rats in the model group and the moxibustion group were subcutaneously injected with 25% D-galactose [125 mg/（kg·bw）] for 40 d continuous;rats in the normal group were injected with saline at the same position for 40 d continuous.Rats in the moxibustion group were given mild moxibustion at bilateral Shenshu（BL 23） at the same time of modeling;rats in the normal group and the model group were only identically grabbed without moxibustion for 40 d.The learning and memory abilities of rats were observed using the Morris water maze at the end of the experiment.Abdominal aorta blood and thymus were collected after water maze experiment.Enzyme-linked immunosorbent assay（ELISA） was used to detect serum corticosterone level,and immunohistochemical method was used to detect the expression of thymus GR.Results：Compared with the normal group,rats in the model group showed that a significantly longer escape latency time（P〈0.01） on the third and the fourth days;number of times crossing the platform in 70 s significantly reduced（P〈0.01）;activity times in the fourth quadrant significantly decreased（P〈0.05）;serum corticosterone levels increased（P〈0.01）;thymus GR expression decreased（P〈0.05）.Compared with the model group,rats in the moxibustion group showed that the escape latency times were significantly shorter on the third,the fourth and the fifth days（P〈0.01,P〈0.05）;number of times crossing the platform in 70 s significantly increased（P〈0.05）;activity times in the fourth quadrant significantly increased（P〈0.05）;serum corticosterone levels decreased（P〈0.05）;thymus GR expression increased（P〈0.05）.Conclusion：Moxibustion could improve the learning and memory abilities of subacute aging rats,down-regulate serum corticosterone levels,and increase thymus GR content.

Specific acupuncture stimulation of Shenshu(BL23)affects sympathetic nervous activity-associated plasma renin concentration changes

OBJECTIVE:To examine whether specific stimulation of Shenshu(BL23)affects sympathetic nervous activity(SNA)-associated plasma renin concentration(PRC).METHODS:Eight healthy volunteers participated in three pattern conditions in random order:control(Cont),stimulation of Shenshu(BL23),and stimulation of sham point(Sham).All participants were initially in the supine position for>60 min,and then remained in the standing position during the experimental procedure to increase SNA.An electrocardiogram was used to calculate low frequency/high frequency(LF/HF)ratio;blood was collected to analyze PRC.RESULTS:The LF/HF ratio was significantly increased in the standing position when compared with the supine position(P<0.01).There was no difference in LF/HF ratio during or after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,the LF/HF ratio was significantly increased in Cont and Sham conditions(P<0.01).There was no difference in PRC after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,there was a significant increase in PRC in the Cont and Sham conditions(Cont P<0.05,Sham P<0.01).CONCLUSION:Our results demonstrated that specific acupuncture stimulation of Shenshu(BL23)in the standing position decreased SNA-associated PRC,which was not observed during acupuncture stimulation of the sham point.

电针肾俞穴对肾阳虚近视豚鼠视网膜M1受体表达的影响

目的:观察电针肾俞穴对肾阳虚近视豚鼠生长发育、视力及视网膜毒蕈碱样乙酰胆碱(mAChR)M1受体表达的影响,为肾阳虚近视的治疗提供依据。方法:将60只豚鼠随机分为正常对照(NC)组、透镜诱导近视(LIM)组、肾阳虚近视假穴(KYDM+SHAM)组、肾阳虚近视电针(KYDM+EA)组,每组15只。NC组不采取干预措施;LIM组豚鼠右眼配戴-6.00 D透镜构建近视模型;其余两组在戴镜基础上连续2周腹腔注射氢化可的松(剂量10 mg/kg)构建肾阳虚近视模型,造模2周后,KYDM+EA组电针豚鼠双侧肾俞穴,KYDM+SHAM组电针豚鼠臀部假穴,共4周。观察造模不同时间各组豚鼠一般情况,测量体质量和肛温,检测双眼屈光度和眼轴长度,检测血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、睾酮(T)、雌二醇(E_(2)),并在造模结束后采用定量聚合酶链反应(Q-PCR)、免疫组织化学和蛋白质印迹法检测视网膜M1受体mRNA和蛋白表达水平。结果:造模2周后,KYDM+SHAM组和KYDM+EA组豚鼠出现毛发枯槁、无光泽及消瘦、拱背眯眼、倦怠懒动、畏寒肢冷等肾阳虚症状,血清FT3、FT4、T水平低于LIM组,E_(2)高于LIM组,体质量、肛温以及右眼屈光度均低于LIM组,眼轴长度长于LIM组,差异均有统计学意义(P<0.05)。电针4周后,KYDM+EA组豚鼠肾阳虚症状有效改善,血清激素水平趋于正常,与KYDM+SHAM组相比,体质量、肛温、右眼屈光度均明显升高,右眼眼轴长度缩短,视网膜M1受体mRNA和蛋白表达水平降低,差异均有统计学意义(P<0.05)。与LIM组相比,KYDM+EA组豚鼠体质量增加,差异有统计学意义(P<0.05);肛温、右眼屈光度和眼轴长度、视网膜M1受体mRNA和蛋白表达水平差异均无统计学意义(P>0.05)。结论:电针肾俞穴有利于促进肾阳虚近视豚鼠生长发育恢复及视力改善,机制可能与降低视网膜中M1受体表达有关。

A study to investigate needle insertion at Shenshu(BL23) to puncture psoas major muscle

OBJECTIVE： It is unknown whether the psoas major muscle, thought to be a key muscle for treatment of lower back pain, can be punctured at Shenshu（BL23）.METHODS： Twelve dissected specimens were used for studying the needling pathway of BL23 by perpendicularly inserting the depth-measuring blade of a vernier caliper at BL23. Dimensions of psoas muscle were measured. Correlation studies were conducted. In addition, our samples were grouped by gender and underlying medical conditions for analysis.RESULTS： Half（50%） of the needle insertions successfully punctured psoas muscle. The mean depth of needle insertion to puncture psoas muscle（D_（min）） in the group with short-term underlying medical conditions was 38.0 mm（interquartile range 29.0–51.8 mm）, approximately 6 mm deeper than 32.0（29.3–42.5） mm in the group with long-term health problems（P = 0.041）. The cross-sectional area（CSA） of psoas muscle in the former group was on average approximately 1.5 times that of the latter group（P = 0.04）. When the data were analysed by gender, the thickness of psoas muscle in the male group was 19.0（6.5–24.0） mm compared to 19.5（5.8–34.8） mm in the female group（P = 0.02）. The age in the female group（P = 0.04） and the body length of the total group（P = 0.04） negatively correlated to D_（min）.CONCLUSION： Needle insertion at BL23 might be able to puncture psoas muscle. Differences in the CSA of psoas muscle and D_（min） were observed in groups with short-term and long-term underlying medical conditions.

针灸治疗急性肾绞痛临床选穴组方规律研究

【目的】通过数据挖掘技术分析临床针灸治疗急性肾绞痛选穴组方的规律。【方法】检索中国知网期刊全文数据库(CNKI)、万方学术期刊全文数据库(Wanfang)、维普中文科技期刊数据库(VIP)、PubMed数据库已经公开发表并收录的关于针灸治疗急性肾绞痛的临床文献,通过严格筛选,采用Microsoft Excel、SPSS 26.0、IBM SPSS Modeler 15.0软件以及其中的Apriori算法对纳入文献进行信息挖掘与分析。【结果】纳入110篇关于针灸治疗急性肾绞痛的临床文献,统计针灸处方共110条,涉及使用腧穴共59个,其中,肾俞、阿是穴和三阴交的使用频次最高;常用经脉为足太阳膀胱经、足太阴脾经、足阳明胃经;腧穴主要分布在下肢与腰背部;从选用穴位类型来看,五输穴、背俞穴、下合穴为首选;双穴组合配伍中,肾俞、阿是穴组合支持度最高;三穴组合配伍中,肾俞、三阴交、阿是穴组合支持度最高;核心腧穴组合是肾俞与阿是穴组合。【结论】针灸治疗急性肾绞痛的腧穴选择和组方是有规律可循的,所总结的选穴和组方规律能够为其临床治疗和科学研究提供参考。

温针灸“肾俞”穴对老年雌性大鼠性激素的影响

目的 :观察温针灸对老年雌性大鼠性激素的影响。方法 :温针灸“肾俞”穴 ,观察其对雌二醇(E2 )、孕酮 (P)、促卵泡生成激素 (FSH)、促黄体生成激素 (LH)的变化。结果 :温针灸“肾俞”组E2 水平明显升高 ,温针灸“肾俞”与尼尔雌醇组FSH水平明显降低 (P <0 0 1) ,P显著升高 (P <0 0 5,P<0 0 1)。温针灸“肾俞”对LH水平的变化影响不大 ,而尼尔雌醇组LH水平则明显低于空白对照组。结论 :温针灸“肾俞”穴具有明显的调节性激素作用 ,可以使明显低下的雌二醇与孕酮水平升高 ,从而建立起卵巢与垂体之间负反馈关系 ,同时 。

俞募穴埋线治疗卵巢早衰疗效观察

目的:观察肝脾肾之俞募穴埋线治疗卵巢早衰的临床疗效。方法:将132例卵巢早衰患者随机分成埋线组和药物组,每组各66例。埋线组给予肝脾肾之俞募穴埋线治疗;药物组采用激素替代疗法,即口服戊酸雌二醇(补佳乐)2 mg,每日1次,连服20天,10天后加服安宫黄体酮4 mg,每日2次,连服10天,平均用药6个月。观察2组疗效及血清促卵泡激素(FSH)和雌二醇(E2)变化。结果:2组治疗后血清FSH和E2均有显著改善(P<0.01),且埋线组血清E2提高较药物组显著(P<0.01)。埋线组痊愈率为84.9%,总有效率为97.0%;药物组分别为31.8%、84.8%。埋线组痊愈率优于药物组(P<0.05)。随访10个月,埋线组疗效可维持。结论:肝脾肾之俞募穴埋线治疗卵巢早衰疗效好,无任何毒副作用,值得临床推广应用。

电针对血管性痴呆大鼠学习记忆能力的改善作用及其机理探讨

【目的】观察电针对血管性痴呆(vascular dementia,VD)大鼠学习记忆能力的影响及其可能的机理。【方法】选择SPF级成年健康SD大鼠50只,随机抽取8只大鼠为假手术组,其他42只均采用4血管阻断法复制缺血模型。将造模成功的大鼠按随机数字表法分为电针组、尼莫通组和模型组。假手术组和模型组同等条件下饲养,未予任何治疗。电针组针刺模型大鼠头部百会穴,背部双侧膈俞穴、脾俞穴和肾俞穴,连接电针仪,施以连续波,频率150 Hz,1次/d,连续治疗15 d。尼莫通组模型大鼠给予尼莫通12 mg/kg,1次/d,连续15 d。治疗15 d后采用Morris水迷宫学习记忆行为测试法测定大鼠学习记忆能力,采用双波长分光光度计法检测大鼠血浆CO含量,分光光度比色法测定缺血脑组织中NO的含量。【结果】模型大鼠表现出明显的学习记忆障碍,在水迷宫实验中,其逃避潜伏期显著性延长,在原平台象限跨越相应平台次数与其他3个象限无显著性差异,血浆CO和脑组织NO水平显著性升高。而电针组逃避潜伏期较模型组显著性缩短,相同时间内跨越原平台次数显著性多于其他3个象限,血浆CO和脑组织NO含量较模型组大鼠显著降低(均P<0.05或P<0.01)。【结论】电针治疗血管性痴呆的作用可能与其改善VD大鼠学习记忆能力,显著性降低血浆CO和脑组织NO含量有关。

肾俞募穴与肾特异性联系通路的荧光双标法研究

目的 :探求肾与其俞募穴之间的神经联系通路。方法 :应用荧光双标记法进行研究。结果 :“肾俞” 肾组、“京门” 肾组、“肾俞” “京门”组分别在T1 3～L3、T1 2 ～L2 、T1 3～L1 脊神经节内出现双标细胞。结论 :①脊神经节中存在可双重支配肾和体表的神经元 ,不仅使牵涉痛的机制得到进一步的解释 ,而且揭示了针刺穴位的感觉冲动是通过分支的传入轴突影响到内脏的功能和感觉或对某些脏器的活动产生影响。②为临床应用肾俞募穴治疗肾病提供了形态学理论根据。③进而揭示中医经络学中气街可能的现代生物学本质。

穴位埋线对更年期雌性大鼠下丘脑单胺类神经递质的影响

【目的】通过观察穴位埋线对更年期大鼠下丘脑去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)等单胺类神经递质的影响,探讨其对更年期综合征防治作用的机理。【方法】选用60只SD雌性大鼠,随机分为治疗组、模型组和假手术组各20只;除假手术组外,其他组均采用手术摘除大鼠双侧卵巢复制去势更年期大鼠模型,治疗组选取肾俞、三阴交、子宫穴进行穴位埋线,1次/周,连续4周;采用放射免疫法检测下丘脑组织NE、DA、5-HT、5-HIAA含量。【结果】模型组较假手术组的NE、DA水平显著降低(P<0.05或P<0.01),5-HT、5-HIAA水平显著上升(P<0.01或P<0.001),5-HT/NE比值显著升高(P<0.001);穴位埋线组与模型组比较,可显著升高衰退的NE水平(P<0.05),降低5-HT、5-HIAA水平(P<0.05或P<0.01),且埋线组的5-HT/NE比值亦较模型组显著降低(P<0.01)。【结论】穴位埋线治疗更年期综合征的作用可能与其能调整去势雌性大鼠下丘脑单胺类神经递质,纠正下丘脑紊乱的植物神经功能有关。

麝香注射液穴位注射治疗视网膜色素变性的眼电图研究

【目的】探讨麝香注射液穴位注射肝俞、肾俞对治疗视网膜色素变性(RP)的作用。【方法】应用TEC-150V+视觉电生理检测仪,对30例60只患眼治疗前后进行眼电图(EOG)的检测。【结果】30例患者60眼EOG的光峰、暗谷振幅以及Q值均较治疗前提高(P<0.01);92％患眼的视力明显提高(P<0.01)。【结论】麝香注射液肝俞、肾俞穴位注射治疗RP,可有效地改善视网膜色素上皮细胞膜的代谢,促进视网膜色素上皮-光感受器复合体的功能,对延缓病情的发展、保护中心视力具有积极的临床意义。

针刺治疗椎动脉型颈椎病70例疗效分析

对 140例椎动脉型颈椎病随机分成针刺组和药物组 ,分别以针刺和药物治疗。结果 :针刺组痊愈显效率与药物组相比差异有非常显著性意义 (P <0 0 1) ,而两组总有效率差异无显著性意义 (P >0 0 5) ;两组在治疗后第 10个月和第 18个月分别进行随访 ,针刺组复发率明显低于药物组(P <0 0 5)。针刺组的近期疗效和远期疗效均优于药物组。

肾俞埋线对绝经后骨质疏松症临床疼痛的影响

目的探讨肾俞穴位埋线治疗绝经后骨质疏松症临床疼痛的有效性。方法将56例患者随机分为埋线组、埋线乐力组和乐力组,以疼痛评分方法评定治疗前、治疗3个月和治疗6个月疼痛情况。结果埋线组、埋线乐力组疼痛评分于治疗前后比较,差异均有极显著性意义(P<0·001),两组间比较,差异无显著性意义(P>0·05);乐力组疼痛评分于治疗前后比较,差异无显著性意义(P>0·05),与埋线组、埋线乐力组分别比较,差异均有极显著性意义(P<0·001)。结论肾俞穴位埋线对绝经后骨质疏松症临床疼痛有非常明显的疗效;口服乐力胶囊对改善绝经后骨质疏松症临床疼痛无显著疗效。