An acute toxicity test in Sprague Dawley(SD)rats and a chromosomal aberration toxicity test in Chinese hamster lung(CHL)fibroblasts were conducted to promote the application of insect wax and its policosanol.Results o...An acute toxicity test in Sprague Dawley(SD)rats and a chromosomal aberration toxicity test in Chinese hamster lung(CHL)fibroblasts were conducted to promote the application of insect wax and its policosanol.Results of oral acute toxicity test showed that the LD;values of insect wax and its policosanol were higher than 5000 mg/kg.The rats showed no obvious toxic symptoms and survived,suggesting that these substances were not toxic.The chromosomal aberration rates of the CHL cells incubated with insect wax for 3-6 h under metabolic activation conditions and for 3-6 h and 24 h under non-metabolic activation conditions were lower than 5%.No significant difference was found compared with the negative control group.Also,no significant difference in chromosomal aberration rates was found between each dose group of policosanol under metabolic or non-metabolic activation conditions and the negative control group.Therefore,the chromosomal aberration rates of CHL cells treated with insect wax and its policosanol were negative regardless of metabolic activation conditions.These results indicate the absence of acute toxicity and potential chromosomal aberration in the tested dose range of insect wax and its policosanol.展开更多
A new and simple gas chromatographic (GC) method using flame ionization detector (FID) and mass spectrometry (MS) for the simultaneous determination of eight fatty alcohols in 10 mg policosanol film-coated tablets was...A new and simple gas chromatographic (GC) method using flame ionization detector (FID) and mass spectrometry (MS) for the simultaneous determination of eight fatty alcohols in 10 mg policosanol film-coated tablets was established and applied to the quality control (QC) of policosanol film-coated tablets. A DB-35MS capillary column (30 m × 0.32 mm, 0.25 um) was employed for the separation. GC-FID was used to quantitatively analyze the eight ingredients with 1-eicosanol as internal standard, three of which were identified using GC-MS due to the lack of standard. The linearity, accuracy, precision, stability, robustness and sensitivity within the detection limits were evaluated. The average recovery of the method was 96.3-100.4% and linearity was (R > 0.999). The average drug content was found to be 96.8% of the labeled amount (10 mg).展开更多
Background:Cardiovascular disease is the world’s number one killer disease and the leading non-communicable disease in terms of causing premature deaths.Overwhelming evidence suggest that effective management of meta...Background:Cardiovascular disease is the world’s number one killer disease and the leading non-communicable disease in terms of causing premature deaths.Overwhelming evidence suggest that effective management of metabolic syndrome will markedly reduce morbidity and premature deaths from cardiovascular disease.Although many therapies exist,most of them are ineffective due to decreased effectiveness and/or side effects following prolonged usage.Policosanol,a well-tolerated long-chain aliphatic alcohol has been proven to be effective against the components of metabolic syndrome(namely dyslipidemia,diabetes,hypertension,and obesity)even when used for a long period with minimal or no adverse effects.Objective:This study intends to explore the potential benefits of Policosanol in the management of metabolic syndrome.Methods:PubMed,Google Scholar,and Science Direct were used as the search engines to retrieve articles related to Policosanol and metabolic syndrome from 2010 to 2021.Results:The results from the three search engines were scrutinized and merged.Duplicate publications were excluded.Similarly,four articles from the WHO website(www.who.int/publications)were included making a total of 63 articles used in this review.Most of the reviewed articles show Policosanol to be effective in reducing systolic and diastolic blood pressure,blood glucose level,body weight,total cholesterol level,triglyceride level,low density lipoprotein and increasing high density lipoprotein levels.There are few conflicting articles that reported Policosanol to have no effect on lipid parameters.Conclusion:Policosanol was shown to be a safe and well-tolerated natural product that is effective against all the components of metabolic syndrome.Thus,using this natural product will go a long way in reducing the burden and economic consequences of the syndrome.展开更多
基金the support of the Fundamental Research Funds for the Central Non-profit Research Institution of CAF(CAFYBB2018SY025)the National High Technology Research and Development Program of China(863 Program)(2014AA021801)。
文摘An acute toxicity test in Sprague Dawley(SD)rats and a chromosomal aberration toxicity test in Chinese hamster lung(CHL)fibroblasts were conducted to promote the application of insect wax and its policosanol.Results of oral acute toxicity test showed that the LD;values of insect wax and its policosanol were higher than 5000 mg/kg.The rats showed no obvious toxic symptoms and survived,suggesting that these substances were not toxic.The chromosomal aberration rates of the CHL cells incubated with insect wax for 3-6 h under metabolic activation conditions and for 3-6 h and 24 h under non-metabolic activation conditions were lower than 5%.No significant difference was found compared with the negative control group.Also,no significant difference in chromosomal aberration rates was found between each dose group of policosanol under metabolic or non-metabolic activation conditions and the negative control group.Therefore,the chromosomal aberration rates of CHL cells treated with insect wax and its policosanol were negative regardless of metabolic activation conditions.These results indicate the absence of acute toxicity and potential chromosomal aberration in the tested dose range of insect wax and its policosanol.
文摘A new and simple gas chromatographic (GC) method using flame ionization detector (FID) and mass spectrometry (MS) for the simultaneous determination of eight fatty alcohols in 10 mg policosanol film-coated tablets was established and applied to the quality control (QC) of policosanol film-coated tablets. A DB-35MS capillary column (30 m × 0.32 mm, 0.25 um) was employed for the separation. GC-FID was used to quantitatively analyze the eight ingredients with 1-eicosanol as internal standard, three of which were identified using GC-MS due to the lack of standard. The linearity, accuracy, precision, stability, robustness and sensitivity within the detection limits were evaluated. The average recovery of the method was 96.3-100.4% and linearity was (R > 0.999). The average drug content was found to be 96.8% of the labeled amount (10 mg).
文摘Background:Cardiovascular disease is the world’s number one killer disease and the leading non-communicable disease in terms of causing premature deaths.Overwhelming evidence suggest that effective management of metabolic syndrome will markedly reduce morbidity and premature deaths from cardiovascular disease.Although many therapies exist,most of them are ineffective due to decreased effectiveness and/or side effects following prolonged usage.Policosanol,a well-tolerated long-chain aliphatic alcohol has been proven to be effective against the components of metabolic syndrome(namely dyslipidemia,diabetes,hypertension,and obesity)even when used for a long period with minimal or no adverse effects.Objective:This study intends to explore the potential benefits of Policosanol in the management of metabolic syndrome.Methods:PubMed,Google Scholar,and Science Direct were used as the search engines to retrieve articles related to Policosanol and metabolic syndrome from 2010 to 2021.Results:The results from the three search engines were scrutinized and merged.Duplicate publications were excluded.Similarly,four articles from the WHO website(www.who.int/publications)were included making a total of 63 articles used in this review.Most of the reviewed articles show Policosanol to be effective in reducing systolic and diastolic blood pressure,blood glucose level,body weight,total cholesterol level,triglyceride level,low density lipoprotein and increasing high density lipoprotein levels.There are few conflicting articles that reported Policosanol to have no effect on lipid parameters.Conclusion:Policosanol was shown to be a safe and well-tolerated natural product that is effective against all the components of metabolic syndrome.Thus,using this natural product will go a long way in reducing the burden and economic consequences of the syndrome.
