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Deep-sea Pollen Record during 3.0-2.0 Ma B.P. from ODP Site 1143 and Its Response to Global Climate Changes 被引量:1
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作者 袁金红 罗运利 +1 位作者 徐兆良 孙湘君 《Marine Science Bulletin》 CAS 2006年第1期1-10,共10页
In order to study vegetation evolution and environmental changes around 2.5 Ma B.P., a total of 146 pollen samples with an average time resolution of 7 000 years were analyzed in the deep-sea record at the depth of 13... In order to study vegetation evolution and environmental changes around 2.5 Ma B.P., a total of 146 pollen samples with an average time resolution of 7 000 years were analyzed in the deep-sea record at the depth of 135 - 95 m (in composition depth) from ODP Site 1143 (9° 22' N, 113° 17' E) in the southern South China Sea. The results show that the pollen influx has a distinct change. During 2.6 - 2.0 Ma B.P., the average value of pollen influx increased evidently compared with that of 3.0 - 2.6 Ma B.P. It shows that the sea level of SCS dropped dramatically around 2.6 Ma B.P., corresponding to the formation of the Northern Hemisphere ice-sheets and the enhancement of the East Asian Monsoon. The pollen influx variations reflect the glacial-interglacial cycles corresponding with the deep-sea oxygen isotope curve and indicate that the sea level of SCS rose and dropped many times after 2.6 Ma B.P. The spectrum analysis results of pollen influx show that there are cycles at 0.1Ma (eccentricity) and 46.9 ka (obliquity) during 3.0 - 2.0 Ma B.P. 展开更多
关键词 pollen influx 3.0 - 2.0 Ma B.P. ODP Site 1143 the South China Sea
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通过改良农杆菌介导的floral-dip法和花粉管通道法转化紫薇 被引量:5
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作者 陈彦 孙宽莹 张涛 《浙江大学学报(农业与生命科学版)》 CAS CSCD 北大核心 2012年第3期250-255,共6页
以绿色荧光蛋白(GFP)基因为报告基因,分别用改良农杆菌介导的花序浸渍法(floral-dip)和花粉管通道法转化紫薇(Lagerstroemia indica),统计果实数、结实率、成苗率,然后用紫外灯照射28d左右的幼苗筛选转化株。结果表明:花粉管通道法转化... 以绿色荧光蛋白(GFP)基因为报告基因,分别用改良农杆菌介导的花序浸渍法(floral-dip)和花粉管通道法转化紫薇(Lagerstroemia indica),统计果实数、结实率、成苗率,然后用紫外灯照射28d左右的幼苗筛选转化株。结果表明:花粉管通道法转化紫薇的结实率、成苗率均明显高于floral-dip法;用手携式紫外灯(365nm)照射进行活体鉴定,floral-dip法转化率比花粉管通道转基因法转化率高0.06%,但未达到显著水平;而花粉管通道法的结实率比floral-dip法的高11.87%~26.74%,更利于获得转化植株。说明通过花粉管通道法进行转化更适合于紫薇转基因新品种的培育。 展开更多
关键词 紫薇 改良农杆菌介导的floral—dip法 花粉管通道法 转化
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桃花粉管通道法转基因技术的初步研究 被引量:7
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作者 万春雁 韩明玉 +3 位作者 赵彩平 侯玉珏 丛芳 田玉命 《中国农学通报》 CSCD 北大核心 2009年第5期38-42,共5页
以曙光为试材,对桃花粉管通道法转基因技术进行探索性研究,结果表明:(1)活体人工授粉后,24 h花粉开始在柱头上萌发授粉后48 h已有大量的花粉管长至花柱上端;授粉后96 h大量的花粉管生长至花柱的中部;授粉后144 h大量的花粉管到达花柱基... 以曙光为试材,对桃花粉管通道法转基因技术进行探索性研究,结果表明:(1)活体人工授粉后,24 h花粉开始在柱头上萌发授粉后48 h已有大量的花粉管长至花柱上端;授粉后96 h大量的花粉管生长至花柱的中部;授粉后144 h大量的花粉管到达花柱基部。(2)确定外源DNA的注射时间为人工授粉后的144 h;花柱横切滴加法注射方式不适用于桃;当外源质粒DNA浓度为0.2 mg/L时,发育期内其坐果率都显著高于其它浓度水平。 展开更多
关键词 花粉管通道法 注射时间 注射方式 注射浓度
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花粉管介导的准噶尔小胸鳖甲抗冻蛋白转基因棉花的筛选 被引量:4
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作者 马纪 李春平 +3 位作者 邱立明 徐建辉 赵干 李素丽 《新疆农业科学》 CAS CSCD 2008年第3期381-385,共5页
利用新疆荒漠昆虫抗冻蛋白基因进行遗传转化是培育新疆棉花抗寒品种的新途径。研究采用RT-PCR结合RACE技术从新疆荒漠昆虫准噶尔小胸鳖甲(Microdera puntipennis dzungarica)中克隆获得抗冻蛋白(Antifreeze protein)基因MpAFP149。将MpA... 利用新疆荒漠昆虫抗冻蛋白基因进行遗传转化是培育新疆棉花抗寒品种的新途径。研究采用RT-PCR结合RACE技术从新疆荒漠昆虫准噶尔小胸鳖甲(Microdera puntipennis dzungarica)中克隆获得抗冻蛋白(Antifreeze protein)基因MpAFP149。将MpAFP149克隆至pBI121上,获得重组质粒后采用电转化法将重组质粒转入农杆菌EHA105内,PCR筛选鉴定重组子,并测序确定抗冻蛋白植物表达载体构建正确。将pBI121-MpAFP通过田间的花粉管导入技术转化棉花,收取转化的棉花种子,经检测证明在转化10 000朵花中,卡那霉素抗性筛选出13株,PCR检测有2株阳性植株。实验结果为进一步开展抗冻蛋白基因转化棉花的研究奠定了基础。 展开更多
关键词 准噶尔小胸鳖甲 抗冻蛋白 基因转化 花粉管导入 棉花
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Pollen wall pattern in Arabidopsis 被引量:8
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作者 Te Xu Cheng Zhang +1 位作者 Que Zhou Zhong-Nan Yang 《Science Bulletin》 SCIE EI CAS CSCD 2016年第11期832-837,共6页
The pollen wall is a solid and variously sculptured structure. This pattern is determined inside a tetrad. During meiosis, the callose wall is formed outside of the meiocyte/microspore to form a tetrad. Then, primexin... The pollen wall is a solid and variously sculptured structure. This pattern is determined inside a tetrad. During meiosis, the callose wall is formed outside of the meiocyte/microspore to form a tetrad. Then, primexine is deposited between the callose wall and the microspore plasma membrane which will become undulated. The sporopollenin deposits on top of the undulated membrane and develops into the pollen wall pattern, while the callose wall is gradually degraded. In recent years, much progress has been made in the study of pollen wall pattern formation, at both molecular and genetic levels. In this review,we summarize these achievements mainly in Arabidopsis. 展开更多
关键词 pollen wall pattern Sexine Callose Primexine - Plasma membrane undulation SPOROpollenIN
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