Phosphorus-containing amendments can reduce the mobility of Pb in soil. Hydroxyapatite (HAP) is one of the most commonly used phosphorus-containing amendments. With the development of nanotechnology, nano-hydroxyapati...Phosphorus-containing amendments can reduce the mobility of Pb in soil. Hydroxyapatite (HAP) is one of the most commonly used phosphorus-containing amendments. With the development of nanotechnology, nano-hydroxyapatie (n-HAP) was gradually applied to remediate soil polluted by heavy metals. Considering the concentrations of HAP/n-HAP were not more than 5% in most studies, soil polluted by Pb was artificially prepared and three different concentrations of n-HAP: 5%, 7% and 10% by weight, were added into the Pb-polluted soil separately. The mixtures of soil and n-HAP were incubated for 180 d and sampled regularly. The bioaccessibility of Pb in soil was determined using simulated gastric juices of two in-vitro digestion tests: USPM (United States Pharmacopeia Methodology) and PBET (Physiologically-Based Extraction Test). The results showed that the immobilizing efficiency of 5% n-HAP to Pb in soil was the highest in PBET. The extractable Pb from soil by USPM was not affected by concentration of n-HAP. So, the least concentration of n-HAP, i.e. 5% n-HAP treatment, was the most cost-effective in USPM. Soil pH increased with concentration of n-HAP. However concentration of n-HAP had little effects on content of soil OM. According to regression analysis, more than 50% differences of the extractable Pb from soil by PBET can be explained by soil pH, while soil pH, organic matter content and incubation time together explained nearly 85% differences of extractable Pb from soil by USPM.展开更多
As widespread wetland plants,Phragmites play a vital role in water purification and are widely utilized in constructed wetlands(accounting for 15.5%of applied wetland plants)as a natural alternative to wastewater trea...As widespread wetland plants,Phragmites play a vital role in water purification and are widely utilized in constructed wetlands(accounting for 15.5%of applied wetland plants)as a natural alternative to wastewater treatment.However,despite such common applications,current understanding of the basic composition of the Phragmites root-inhabiting microbiome and the complex functions of each member of this microbiome remains incomplete,especially regarding pollution remediation.This review summa-rizes the advances that have been made in ecological and biochemical research on the Phragmites root microbiome,including bacteria,archaea,and fungi.Based on next-generation sequencing,microbial com-munity compositions have been profiled under various environmental conditions.Furthermore,culture-based methods have helped to clarify the functions of the microbiome,such as metal iron stabilization,organic matter degradation,and nutrient element transformation.The unique community structure and functions are highly impacted by Phragmites lineages and environmental factors such as salinity.Based on the current understanding of the Phragmites root microbiome,we propose that synthetic microbial com-munities and iron–manganese plaque could be applied and intensified in constructed wetlands to help promote their water purification performance.展开更多
financially supported by the Key R&D Program of Jiangxi Province of China (20152ACF60010);the Key Projects of Hunan Science and Technology Support Program of China (Application research of Guiye A male sterile lin...financially supported by the Key R&D Program of Jiangxi Province of China (20152ACF60010);the Key Projects of Hunan Science and Technology Support Program of China (Application research of Guiye A male sterile line);the Research Project of Humanities and Social Sciences in Universities of Jiangxi Province, China(JC1315);supported by Emmy Noether DFG grant MA 6473/1-1展开更多
We studied the dynamics of mercury(Hg) transfer in Phaseolus vulgaris plants grown in soil with Hg-doped compost at the maximum levels permitted by Colombian law on organic amendments. Quantitative evaluation of tra...We studied the dynamics of mercury(Hg) transfer in Phaseolus vulgaris plants grown in soil with Hg-doped compost at the maximum levels permitted by Colombian law on organic amendments. Quantitative evaluation of transfer was made in different plant organs: roots,stem, leaves, pods and seeds. Matrix effect was determined in doped soil assays, using soil with and without addition of compost. Results showed that the use of organic matter reduced Hg transfer to the plant and the amount transferred was differentially distributed to the organs. We observed an inverse relationship between concentration and distance from the body to the root. It was evident that transfer was mediated by quantitative factors;the greater the presence of mercury in soil, the larger the amount that will be transferred.Results also indicate the remedial effect of compost and the presence of a barrier, at the root level, against mercury translocation to the plant aerial parts.展开更多
Chlorinated persistent organic pollutants, including polychlorinated biphenyls (PCBs), represent a particularly serious environmental problem and human health risk worldwide. Leguminous plants and their symbiotic ba...Chlorinated persistent organic pollutants, including polychlorinated biphenyls (PCBs), represent a particularly serious environmental problem and human health risk worldwide. Leguminous plants and their symbiotic bacteria (rhizobia) are important components of the biogeochemical cycling of nitrogen in both agricultural and natural ecosystems. However, there have been relatively few detailed studies of the remediation of PCB-contaminated soils by legume-rhizobia symbionts. Here we report for the first time evidence of the reductive dechlorination of 2,4,4'-trichlorobiphenyl (PCB 28) by an alfalfa-rhizobium nitrogen fixing symbiont. Alfalfa (Medicago sativa L.) inoculated with wild-type Sinorhizobiurn meliloti had significantly larger biomass and PCB 28 accumulation than alfalfa inoculated with the nitrogenase negative mutant rhizobium SmY. Dechlorination products of PCB 28, 2,4'-dichlorobiphenyl (PCB 8), and the emission of chloride ion (C1-) were also found to decrease significantly in the ineffective nodules infected by the mutant strain SmY. We therefore hypothesize that N2-fixation by the legume-rhizobium symbiont is coupled with the reductive dechlorination of PCBs within the nodules. The combination of these two processes is of great importance to the biogeochemical cycling and bioremediation of organochlorine pollutants in terrestrial ecosystems.展开更多
文摘Phosphorus-containing amendments can reduce the mobility of Pb in soil. Hydroxyapatite (HAP) is one of the most commonly used phosphorus-containing amendments. With the development of nanotechnology, nano-hydroxyapatie (n-HAP) was gradually applied to remediate soil polluted by heavy metals. Considering the concentrations of HAP/n-HAP were not more than 5% in most studies, soil polluted by Pb was artificially prepared and three different concentrations of n-HAP: 5%, 7% and 10% by weight, were added into the Pb-polluted soil separately. The mixtures of soil and n-HAP were incubated for 180 d and sampled regularly. The bioaccessibility of Pb in soil was determined using simulated gastric juices of two in-vitro digestion tests: USPM (United States Pharmacopeia Methodology) and PBET (Physiologically-Based Extraction Test). The results showed that the immobilizing efficiency of 5% n-HAP to Pb in soil was the highest in PBET. The extractable Pb from soil by USPM was not affected by concentration of n-HAP. So, the least concentration of n-HAP, i.e. 5% n-HAP treatment, was the most cost-effective in USPM. Soil pH increased with concentration of n-HAP. However concentration of n-HAP had little effects on content of soil OM. According to regression analysis, more than 50% differences of the extractable Pb from soil by PBET can be explained by soil pH, while soil pH, organic matter content and incubation time together explained nearly 85% differences of extractable Pb from soil by USPM.
基金supported by the National Natural Science Foundation of China (51778603)the Chinese Academy of Sciences(QYZDY-SSW-DQC004)
文摘As widespread wetland plants,Phragmites play a vital role in water purification and are widely utilized in constructed wetlands(accounting for 15.5%of applied wetland plants)as a natural alternative to wastewater treatment.However,despite such common applications,current understanding of the basic composition of the Phragmites root-inhabiting microbiome and the complex functions of each member of this microbiome remains incomplete,especially regarding pollution remediation.This review summa-rizes the advances that have been made in ecological and biochemical research on the Phragmites root microbiome,including bacteria,archaea,and fungi.Based on next-generation sequencing,microbial com-munity compositions have been profiled under various environmental conditions.Furthermore,culture-based methods have helped to clarify the functions of the microbiome,such as metal iron stabilization,organic matter degradation,and nutrient element transformation.The unique community structure and functions are highly impacted by Phragmites lineages and environmental factors such as salinity.Based on the current understanding of the Phragmites root microbiome,we propose that synthetic microbial com-munities and iron–manganese plaque could be applied and intensified in constructed wetlands to help promote their water purification performance.
基金financially supported by the Key R&D Program of Jiangxi Province of China (20152ACF60010)the Key Projects of Hunan Science and Technology Support Program of China (Application research of Guiye A male sterile line)+1 种基金the Research Project of Humanities and Social Sciences in Universities of Jiangxi Province, China(JC1315)supported by Emmy Noether DFG grant MA 6473/1-1
文摘financially supported by the Key R&D Program of Jiangxi Province of China (20152ACF60010);the Key Projects of Hunan Science and Technology Support Program of China (Application research of Guiye A male sterile line);the Research Project of Humanities and Social Sciences in Universities of Jiangxi Province, China(JC1315);supported by Emmy Noether DFG grant MA 6473/1-1
基金the University of Antioquia (UdeA ) for partially funding this research (grant CODI-10083-2007)the BIOORGANICOS S.A.Company for participating in the project and supplying organic substratesthe research group GAIA-UdeA for access to the mercury analyzer
文摘We studied the dynamics of mercury(Hg) transfer in Phaseolus vulgaris plants grown in soil with Hg-doped compost at the maximum levels permitted by Colombian law on organic amendments. Quantitative evaluation of transfer was made in different plant organs: roots,stem, leaves, pods and seeds. Matrix effect was determined in doped soil assays, using soil with and without addition of compost. Results showed that the use of organic matter reduced Hg transfer to the plant and the amount transferred was differentially distributed to the organs. We observed an inverse relationship between concentration and distance from the body to the root. It was evident that transfer was mediated by quantitative factors;the greater the presence of mercury in soil, the larger the amount that will be transferred.Results also indicate the remedial effect of compost and the presence of a barrier, at the root level, against mercury translocation to the plant aerial parts.
基金supported by the National Natural Science Foundation of China(Grant Nos.41201313&41230858)
文摘Chlorinated persistent organic pollutants, including polychlorinated biphenyls (PCBs), represent a particularly serious environmental problem and human health risk worldwide. Leguminous plants and their symbiotic bacteria (rhizobia) are important components of the biogeochemical cycling of nitrogen in both agricultural and natural ecosystems. However, there have been relatively few detailed studies of the remediation of PCB-contaminated soils by legume-rhizobia symbionts. Here we report for the first time evidence of the reductive dechlorination of 2,4,4'-trichlorobiphenyl (PCB 28) by an alfalfa-rhizobium nitrogen fixing symbiont. Alfalfa (Medicago sativa L.) inoculated with wild-type Sinorhizobiurn meliloti had significantly larger biomass and PCB 28 accumulation than alfalfa inoculated with the nitrogenase negative mutant rhizobium SmY. Dechlorination products of PCB 28, 2,4'-dichlorobiphenyl (PCB 8), and the emission of chloride ion (C1-) were also found to decrease significantly in the ineffective nodules infected by the mutant strain SmY. We therefore hypothesize that N2-fixation by the legume-rhizobium symbiont is coupled with the reductive dechlorination of PCBs within the nodules. The combination of these two processes is of great importance to the biogeochemical cycling and bioremediation of organochlorine pollutants in terrestrial ecosystems.