A photonic lab on a chip(PhLOC),comprising a solid-state light emitter(SSLE)aligned with a biofunctionalized optofluidic multiple internal reflection(MIR)system,is presented.The SSLE is obtained by filling a microflui...A photonic lab on a chip(PhLOC),comprising a solid-state light emitter(SSLE)aligned with a biofunctionalized optofluidic multiple internal reflection(MIR)system,is presented.The SSLE is obtained by filling a microfluidic structure with a phenyltrimethoxysilane(PhTMOS)aqueous sol solution containing a fluorophore organic dye.After curing,the resulting xerogel solid structure retains the emitting properties of the fluorophore,which is evenly distributed in the xerogel matrix.Photostability studies demonstrate that after a total dose(at λ5365 nm)greater than 24 J cm^(-2),the xerogel emission decay is only 4.1%.To re-direct the emitted light,the SSLE includes two sets of air mirrors that surround the xerogel.Emission mapping of the SSLE demonstrates that alignment variations of 150 mm(between the SSLE and the external pumping light source)provide fluctuations in emitted light smaller than 5%.After this verification,the SSLE is monolithically implemented with a MIR,forming the PhLOC.Its performance is assessed by measuring quinolone yellow,obtaining a limit of detection(LOD)of(0.6060.01)mM.Finally,the MIR is selectively biofunctionalized with horseradish peroxidase(HRP)for the detection of hydrogen peroxide(H_(2)O_(2))target analyte,obtaining a LOD of(0.760.1)μM for H_(2)O_(2),confirming,for the first time,that solid-state xerogel-based emitters can be massively implemented in biofunctionalized PhLOCs.展开更多
基金The research leading to these results has received funding from the European Research Council under the European Community’s Seventh Framework Programme(FP7/2007-2013)/ERC grant agreement no.209243 and Spanish MINECO,project ref.TEC2010-17274.AG acknowledges the support received by CONACyTSB gratefully acknowledges the financial support received by the Volkswagen Foundation.
文摘A photonic lab on a chip(PhLOC),comprising a solid-state light emitter(SSLE)aligned with a biofunctionalized optofluidic multiple internal reflection(MIR)system,is presented.The SSLE is obtained by filling a microfluidic structure with a phenyltrimethoxysilane(PhTMOS)aqueous sol solution containing a fluorophore organic dye.After curing,the resulting xerogel solid structure retains the emitting properties of the fluorophore,which is evenly distributed in the xerogel matrix.Photostability studies demonstrate that after a total dose(at λ5365 nm)greater than 24 J cm^(-2),the xerogel emission decay is only 4.1%.To re-direct the emitted light,the SSLE includes two sets of air mirrors that surround the xerogel.Emission mapping of the SSLE demonstrates that alignment variations of 150 mm(between the SSLE and the external pumping light source)provide fluctuations in emitted light smaller than 5%.After this verification,the SSLE is monolithically implemented with a MIR,forming the PhLOC.Its performance is assessed by measuring quinolone yellow,obtaining a limit of detection(LOD)of(0.6060.01)mM.Finally,the MIR is selectively biofunctionalized with horseradish peroxidase(HRP)for the detection of hydrogen peroxide(H_(2)O_(2))target analyte,obtaining a LOD of(0.760.1)μM for H_(2)O_(2),confirming,for the first time,that solid-state xerogel-based emitters can be massively implemented in biofunctionalized PhLOCs.
