Determination of Total Polyphenols in Different Extraction Fractions of Pholidota chinensis Lindl.and Their Antioxidant Activity

[Objectives]This study was conducted to study the total polyphenol content and antioxidant capacity of different extraction fractions of Pholidota chinensis.[Methods]Different polar fractions of P.chinensis were obtained by ultrasonic extraction method,and determined for polyphenol contents by UV spectrophotometry,and their antioxidant activity was evaluated by DPPH free radical scavenging and ferric ion reducing antioxidant power(FRAP).SPSS analysis software was used to analyze the correlation between total polyphenol contents and antioxidant activity of different extracts.[Results]The contents of total polyphenols in different polar fractions ranged from 1.52 to 6.41 mg/g,and the order from low to high was:water<petroleum ether<ethyl acetate<95%ethanol.The reduction capacity of the total antioxidant capacity method ranged from 12.54 to 22.45μmol/L,and the order was 95%ethanol>ethyl acetate>petroleum ether>water.The DPPH median inhibition concentration ranged from 0.35 to 2.65 mg/ml,and the order was water>petroleum ether>ethyl acetate>95%ethanol.The total polyphenol content of P.chinensis was best in the 95%ethanol extraction fraction,which showed the strongest total antioxidant capacity,so the total polyphenol content of the 95%ethanol fraction was positively correlated with its antioxidant capacity,while the DPPH free radical-scavenging capacity was better in the water fraction,indicating that the free radical-scavenging capacity of the water fraction was better.[Conclusions]This study provides a theoretical basis for the further study of the chemical components of P.chinensis and the development and utilization of natural antioxidants.

Bongardia chrysogonum(L.)Spach:a valuable source for nutraceutical and pharmacological industries

Background:Bongardia chrysogonum(L.)Spach,also known as“Uruf-el-Deek”,has been used by rural communities for the treatment of various diseases and disorders,owing to its wide array of secondary metabolite compositions.Methods:The current study was conducted to determine the total polyphenolic content of Bongardia chrysogonum(L.)Spach as well as its potential free radical scavenging and metal-chelating antioxidant capacities.Its leaves,stems,and tubers were separately extracted using ethanol,water,and chloroform;their antioxidant activities were analyzed using in vitro models,including DPPH,ABTS,FRAP,and CUPRAC assays.The total polyphenolic content of the extracts was spectrophotometrically measured.Results:The richest polyphenolic content of the extracts was yielded by tuber chloroform and leaf ethanol extraction(215.09±0.53 mg/g extract as gallic acid equivalent and 240.74±1.12 mg/g extract as quercetin equivalent,respectively).Almost all of the tested extracts exhibited remarkable antioxidant activities,with the highest antioxidant capacity observed in the leaf extracts.On the other hand,the tuber ethanol extract demonstrated the weakest DPPH radical scavenging activity(%inhibition=49.75±0.38,P<0.01),while the tuber chloroform extract showed the weakest ABTS radical scavenging activity(%inhibition=62.61±0.06,P<0.05).These were closely followed by the tuber water extract(%inhibition=63.28±0.65,P<0.01).In terms of their FRAP and CUPRAC assays,the leaf ethanol extract was determined as the best one,with absorbance values of 3.64±0.01 and 2.22±0.01(P<0.01),respectively.Conclusion:Bongardia chrysogonum(L.)Spach possesses rich polyphenolic content and strong antioxidant activities.Thus,it could be a valuable source for the pharmaceutical industry in the prevention and treatment of diseases associated with oxidative stress.