Portulaca oleracea(P.oleracea)is a traditional Chinese herbal that has the effects of soothing the liver and clearing collaterals,strengthening the spleen and stomach,moistening the intestines and detoxifying.With the...Portulaca oleracea(P.oleracea)is a traditional Chinese herbal that has the effects of soothing the liver and clearing collaterals,strengthening the spleen and stomach,moistening the intestines and detoxifying.With the development of the pharmaceutical industry,the medicinal value of P.oleracea is becoming increasingly prominent.It is commonly used in clinical practice for clearing heat and detoxifying,inhibiting bacteria,preventing and treating diseases such as hypertension,coronary heart disease,and cerebral infarction,indicating its broad application prospects.This study reviews the chemical components and pharmacological activities of P.oleracea in recent decades.展开更多
With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oler...With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oleracea were Optimized. The results showed that there were 8 primers suitable for ISSR-PCR of P. oleracea. The optimal reaction system had a volume of 25 μl, including 2 x Taq Platinum PCR Master Mix 12.5 μl, primer 2 μl, ddH20 9.5 μl, and DNA template 1μl. The optimized ISSR-PCR of P. oleracea was started with pre-denaturation at 94 ℃ for 360 s, followed by 30 cycles of denaturation at 94 ℃ for 60 s, annealing at 54 ℃ for 60 s and extension at 72 ℃ for 90 s, and completed by extension at 72 ℃ for 300 s.展开更多
Portulaca oleracea leaves are tested as an agriculture adsorbent material for reducing Pb(II) from aqueous solutions. The pH of solution, adsorbent dose, shaking speed and particles size of adsorbent were constants at...Portulaca oleracea leaves are tested as an agriculture adsorbent material for reducing Pb(II) from aqueous solutions. The pH of solution, adsorbent dose, shaking speed and particles size of adsorbent were constants at all adsorption experiments. Effects of contact time on adsorption capacity of Pb(II) onto Portulaca oleracea leaves were studied. The adsorption capacity increased as the contact time increased and reached equilibrium at one hour. Kinetic models including a first-order, pseudo-second-order and intra-particle diffusion equations were selected to follow the adsorption process. The process follows a pseudo-second-order kinetic and the intra-particle diffusion is the main step to interpret the mechanism of adsorption. Langmuir, Freundlich and Dubinin-Radush Kevich (D-R) isotherm models were applied to describe adsorption equilibrium data. Results proved that the Langmuir isotherm model gave an acceptable fit to the experimental data more than Freundlich isotherm model. Maximum adsorption capacities obtained were 192.3, 333.3 and 434 mg/g at 295, 303 and 310 K, respectively. According to D-R isotherm data, the adsorption process is classified as physical adsorption. Thermodynamically, the adsorption process is non spontaneous, endothermic and random in nature.展开更多
Objective: Acute liver injury(ALF) is a potential factor of many serious hepatopathies. Carbon tetrachloride(CCl4) is a possible environmental toxicant that can induce ALF. Portulaca oleracea(PO) is one of the most po...Objective: Acute liver injury(ALF) is a potential factor of many serious hepatopathies. Carbon tetrachloride(CCl4) is a possible environmental toxicant that can induce ALF. Portulaca oleracea(PO) is one of the most popular edible herbs and has several biological activities such as antioxidant, antimicrobial, antiinflammatory effects. We explored the significance of PO in regulating inflammatory function in animal models and cultured hepatocytes during liver damage caused by CCl4.Methods: The effect of PO on ALF was evaluated by CCl4-induced mice models in vivo. Hepatic levels of transaminase activities and inflammatory factors were examined. The gene and protein expression of S100A8 and S100A9 were measured by RT-PCR and Western blot analysis. Meanwhile, the efficacy of PO was certified by HepG2 cells in vitro. The transaminase activities, inflammatory factors, and the protein expression of S100A8 and S100A9 were also detected.Results: Animal tests showed that pretreatment with PO reduced the liver pathological tissue damage and the serum levels of ALT, AST, ALT and LDH, as well as reducing the pro-inflammatory cytokines(IL-1β, IL-6, TNF-a) secretion in CCl4-induced liver injury mice. Simultaneously, Hep G2 cells pretreated with PO exhibited a significant decrease in the activities of ALT and AST. Moreover, PO resulted in a significant downregulation of the pro-inflammatory markers S100A8, S100A9 gene and protein expression on CCl4induced acute liver injury was demonstrated entirely in vivo and vitro experiments.Conclusion: PO may down-regulate S100A8 and S100A9 and inhibit pro-inflammatory cytokines’ release,indicating a potential clinical effect for controlling the disease.展开更多
Objective:To investigate the role of Portulaca oleracea(POL)in promoting revascularization and reepithelization as well as inhibiting iron aggregation and inflammation of deep tissue pressure injury(DTPI).Methods:The ...Objective:To investigate the role of Portulaca oleracea(POL)in promoting revascularization and reepithelization as well as inhibiting iron aggregation and inflammation of deep tissue pressure injury(DTPI).Methods:The hydroalcoholic extract of POL(P)and aqueous phase fraction of POL(PD)were prepared based on maceration and liquid–liquid extraction.The number of new blood vessels and VEGF-A expression level were assessed using H&E stain and Western blot on injured muscle to examine the role of POL different extracts in vascularization.The iron distribution and total elemental iron of injured muscle were detected using laser ablation inductively coupled plasma mass spectrometry(ICP-MS)and Perls’staining to determine whether POL extracts can inhibit the iron accumulation.Besides,the ability of POL extracts to promote wound healing by combining re-epithelization time,inflammation degree and collagen deposition area were comprehensively evaluated.Results:In vitro,we observed a significant increase in HUVEC cell viability,migration rate and the number of the tube after P and PD treatment(P<0.05).In vivo,administration of P and PD impacted vascularization and iron accumulation on injured tissue,evident from more new blood vessels,higher expression of VEGF-A and decreased muscle iron concentration of treatment groups compared with no-treatment groups(P<0.05).Besides,shorter re-epithelization time,reduced inflammatory infiltration and distinct collagen deposition were associated with administration of P and PD(P<0.05).Conclusion:POL extract administration groups have high-quality wound healing,which is associated with increased new blood vessels,collagen deposition and re-epithelization,along with decreased iron accumulation and inflammatory infiltration.Our results suggest that that POL extract is beneficial to repair injured muscle after ischemia–reperfusion,highlighting the potential of POL in the DTPI treatment.展开更多
Background:Mosquito is a big threat to the human health.Mosquito-borne diseases cause millions of death to the human beings.Hence,a permanent solution is eagerly to be established to control its excessive growth in st...Background:Mosquito is a big threat to the human health.Mosquito-borne diseases cause millions of death to the human beings.Hence,a permanent solution is eagerly to be established to control its excessive growth in stagnant water.Portulaca oleracea Linn is a natural larvicidal agent,which contains active ingredients such as linolenic acid,linoleic acid(omega-3 fatty acids).These bioactive compounds may be responsible for its larvicidal properties on mosquito.Objective:The present study is focussed on identifying the bioactive compounds,such as linolenic acid,through GC-MS,and analyzing the larvicidal efficacy of Portulaca oleracea L.against Culex quinquefasciatus and Anopheles stephensi larvae.Methods:Preliminary phytochemical analysis,total protein content,total carbohydrate content,total phenol content,total flavonoid content and GC-MS analysis exhibited the presence of rich phytoconstituents in Portulaca oleracea.DPPH analysis was carried out to analyze the antioxidant potential of plant extract.Larvicidal activity and histological change were detected to evaluate the efficacy of Portulaca oleracea L.on the Culex quinquefasciatus and Anopheles stephensi larvae.Results:Aqueous and ethanol leaf extracts of Portulaca oleracea L.against both Culex quinquefasciatus and Anophe-les stephensi larvae,showed a very good larvicidal activity at 500μg/mL among the various concentrations.His-tological damages of mosquito larvae were observed when treated with the Portulaca oleracea L.extract,and provided further evidence for its larvicidal activity.Conclusion:This study concluded that the plant Portulaca oleracea L.contained many useful bioactive compounds,can be a strong larvicidal agent against both Culex quinquefasciatus and Anopheles stephensi larvae.The molecular mechanism for the larvicidal activity will be identified in future studies.展开更多
Portulaca oleracea, a plant species used as medicine and food, is widely spread in most areas of China. It is also a popular food in some Europe and Mediterranean countries. Purslane contains a variety of chemical con...Portulaca oleracea, a plant species used as medicine and food, is widely spread in most areas of China. It is also a popular food in some Europe and Mediterranean countries. Purslane contains a variety of chemical constituents with pharmacological activities, such as antitumor, hypoglycemic, antioxidant, antibacterial,anti-inflammatory activities. It is also full of plenty of nutrients serving as a diet food or healthy food.Here we reviewed purslane in the following aspects: botanical resources, ethnopharmacological function,chemical constituents, pharmacology and pharmacokinetics, safety evaluation and toxicity, and clinical applications.展开更多
基金This work was financially supported by National Nature Science Foundation of China(81973284)Scientific Research Foundation of the Education Department of Liaoning Province(LJKZ0944).
文摘Portulaca oleracea(P.oleracea)is a traditional Chinese herbal that has the effects of soothing the liver and clearing collaterals,strengthening the spleen and stomach,moistening the intestines and detoxifying.With the development of the pharmaceutical industry,the medicinal value of P.oleracea is becoming increasingly prominent.It is commonly used in clinical practice for clearing heat and detoxifying,inhibiting bacteria,preventing and treating diseases such as hypertension,coronary heart disease,and cerebral infarction,indicating its broad application prospects.This study reviews the chemical components and pharmacological activities of P.oleracea in recent decades.
文摘With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oleracea were Optimized. The results showed that there were 8 primers suitable for ISSR-PCR of P. oleracea. The optimal reaction system had a volume of 25 μl, including 2 x Taq Platinum PCR Master Mix 12.5 μl, primer 2 μl, ddH20 9.5 μl, and DNA template 1μl. The optimized ISSR-PCR of P. oleracea was started with pre-denaturation at 94 ℃ for 360 s, followed by 30 cycles of denaturation at 94 ℃ for 60 s, annealing at 54 ℃ for 60 s and extension at 72 ℃ for 90 s, and completed by extension at 72 ℃ for 300 s.
文摘Portulaca oleracea leaves are tested as an agriculture adsorbent material for reducing Pb(II) from aqueous solutions. The pH of solution, adsorbent dose, shaking speed and particles size of adsorbent were constants at all adsorption experiments. Effects of contact time on adsorption capacity of Pb(II) onto Portulaca oleracea leaves were studied. The adsorption capacity increased as the contact time increased and reached equilibrium at one hour. Kinetic models including a first-order, pseudo-second-order and intra-particle diffusion equations were selected to follow the adsorption process. The process follows a pseudo-second-order kinetic and the intra-particle diffusion is the main step to interpret the mechanism of adsorption. Langmuir, Freundlich and Dubinin-Radush Kevich (D-R) isotherm models were applied to describe adsorption equilibrium data. Results proved that the Langmuir isotherm model gave an acceptable fit to the experimental data more than Freundlich isotherm model. Maximum adsorption capacities obtained were 192.3, 333.3 and 434 mg/g at 295, 303 and 310 K, respectively. According to D-R isotherm data, the adsorption process is classified as physical adsorption. Thermodynamically, the adsorption process is non spontaneous, endothermic and random in nature.
基金supported by Independent Research Projects for young teachers of Minzu University of China [No. 2021NQPY90]。
文摘Objective: Acute liver injury(ALF) is a potential factor of many serious hepatopathies. Carbon tetrachloride(CCl4) is a possible environmental toxicant that can induce ALF. Portulaca oleracea(PO) is one of the most popular edible herbs and has several biological activities such as antioxidant, antimicrobial, antiinflammatory effects. We explored the significance of PO in regulating inflammatory function in animal models and cultured hepatocytes during liver damage caused by CCl4.Methods: The effect of PO on ALF was evaluated by CCl4-induced mice models in vivo. Hepatic levels of transaminase activities and inflammatory factors were examined. The gene and protein expression of S100A8 and S100A9 were measured by RT-PCR and Western blot analysis. Meanwhile, the efficacy of PO was certified by HepG2 cells in vitro. The transaminase activities, inflammatory factors, and the protein expression of S100A8 and S100A9 were also detected.Results: Animal tests showed that pretreatment with PO reduced the liver pathological tissue damage and the serum levels of ALT, AST, ALT and LDH, as well as reducing the pro-inflammatory cytokines(IL-1β, IL-6, TNF-a) secretion in CCl4-induced liver injury mice. Simultaneously, Hep G2 cells pretreated with PO exhibited a significant decrease in the activities of ALT and AST. Moreover, PO resulted in a significant downregulation of the pro-inflammatory markers S100A8, S100A9 gene and protein expression on CCl4induced acute liver injury was demonstrated entirely in vivo and vitro experiments.Conclusion: PO may down-regulate S100A8 and S100A9 and inhibit pro-inflammatory cytokines’ release,indicating a potential clinical effect for controlling the disease.
基金supported by the Youth Program of the National Natural Science Foundation of China[grant No.8170,1838]。
文摘Objective:To investigate the role of Portulaca oleracea(POL)in promoting revascularization and reepithelization as well as inhibiting iron aggregation and inflammation of deep tissue pressure injury(DTPI).Methods:The hydroalcoholic extract of POL(P)and aqueous phase fraction of POL(PD)were prepared based on maceration and liquid–liquid extraction.The number of new blood vessels and VEGF-A expression level were assessed using H&E stain and Western blot on injured muscle to examine the role of POL different extracts in vascularization.The iron distribution and total elemental iron of injured muscle were detected using laser ablation inductively coupled plasma mass spectrometry(ICP-MS)and Perls’staining to determine whether POL extracts can inhibit the iron accumulation.Besides,the ability of POL extracts to promote wound healing by combining re-epithelization time,inflammation degree and collagen deposition area were comprehensively evaluated.Results:In vitro,we observed a significant increase in HUVEC cell viability,migration rate and the number of the tube after P and PD treatment(P<0.05).In vivo,administration of P and PD impacted vascularization and iron accumulation on injured tissue,evident from more new blood vessels,higher expression of VEGF-A and decreased muscle iron concentration of treatment groups compared with no-treatment groups(P<0.05).Besides,shorter re-epithelization time,reduced inflammatory infiltration and distinct collagen deposition were associated with administration of P and PD(P<0.05).Conclusion:POL extract administration groups have high-quality wound healing,which is associated with increased new blood vessels,collagen deposition and re-epithelization,along with decreased iron accumulation and inflammatory infiltration.Our results suggest that that POL extract is beneficial to repair injured muscle after ischemia–reperfusion,highlighting the potential of POL in the DTPI treatment.
文摘Background:Mosquito is a big threat to the human health.Mosquito-borne diseases cause millions of death to the human beings.Hence,a permanent solution is eagerly to be established to control its excessive growth in stagnant water.Portulaca oleracea Linn is a natural larvicidal agent,which contains active ingredients such as linolenic acid,linoleic acid(omega-3 fatty acids).These bioactive compounds may be responsible for its larvicidal properties on mosquito.Objective:The present study is focussed on identifying the bioactive compounds,such as linolenic acid,through GC-MS,and analyzing the larvicidal efficacy of Portulaca oleracea L.against Culex quinquefasciatus and Anopheles stephensi larvae.Methods:Preliminary phytochemical analysis,total protein content,total carbohydrate content,total phenol content,total flavonoid content and GC-MS analysis exhibited the presence of rich phytoconstituents in Portulaca oleracea.DPPH analysis was carried out to analyze the antioxidant potential of plant extract.Larvicidal activity and histological change were detected to evaluate the efficacy of Portulaca oleracea L.on the Culex quinquefasciatus and Anopheles stephensi larvae.Results:Aqueous and ethanol leaf extracts of Portulaca oleracea L.against both Culex quinquefasciatus and Anophe-les stephensi larvae,showed a very good larvicidal activity at 500μg/mL among the various concentrations.His-tological damages of mosquito larvae were observed when treated with the Portulaca oleracea L.extract,and provided further evidence for its larvicidal activity.Conclusion:This study concluded that the plant Portulaca oleracea L.contained many useful bioactive compounds,can be a strong larvicidal agent against both Culex quinquefasciatus and Anopheles stephensi larvae.The molecular mechanism for the larvicidal activity will be identified in future studies.
基金supported by National Standardization Project of Traditional Chinese Medicine (No. ZYBZH-C-JL-25)
文摘Portulaca oleracea, a plant species used as medicine and food, is widely spread in most areas of China. It is also a popular food in some Europe and Mediterranean countries. Purslane contains a variety of chemical constituents with pharmacological activities, such as antitumor, hypoglycemic, antioxidant, antibacterial,anti-inflammatory activities. It is also full of plenty of nutrients serving as a diet food or healthy food.Here we reviewed purslane in the following aspects: botanical resources, ethnopharmacological function,chemical constituents, pharmacology and pharmacokinetics, safety evaluation and toxicity, and clinical applications.