Explosive synchronization in a mobile network in the presence of a positive feedback mechanism

Synchronization is a process that describes the coherent dynamics of a large ensemble of interacting units.The study of explosive synchronization transition attracts considerable attention.Here,I report the explosive transition within the framework of a mobile network,while each oscillator is controlled by global-order parameters of the system.Using numerical simulation,I find that the explosive synchronization(ES)transition behavior can be controlled by simply adjusting the fraction of controlled oscillators.The influences of some parameters on explosive synchronization are studied.Moreover,due to the presence of the positive feedback mechanism,I prevent the occurrence of the synchronization of continuous-phase transition and make phase transition of the system a first-order phase transition accompanied by a hysteresis loop.

Experimental study of delayed positive feedback control for a flexible beam

Recently, some researches indicate that positive feedback can benefit the control if appropriate time delay is intentionally introduced into control system. However, most work is theoretical one but few are experimental. This paper presents theoretical and experimental studies of delayed positive feedback control technique using a flexible beam as research object. The positive feedback weighting coefficient is designed by using the optimal control method. The available time delay is determined by analyzing the maximal real part of characteristic roots of the system. A DSP-based experiment system is introduced. Simulation and experimental results indicate that the delayed positive feedback control may effectively reduce the beam vibration if time delay is appropriately selected.

Improvement of a highly sensitive and specific whole-cell biosensor by adding a positive feedback amplifier

In this study,we designed a Cd^(2+)whole-cell biosensor with both positive and negative feedback cascade am-plifiers in Pseudomonas putida KT2440(LTCM)based on our previous design with only a negative feedback amplifier(TCM).The results showed that the newly developed biosensor LTCM was greatly improved compared to TCM.Firstly,the linear response range of LTCM was expanded while the maximum linear response range was raised from 0.05 to 0.1μM.Meanwhile,adding a positive feedback amplifier further increased the fluorescence output signal of LTCM 1.11–2.64 times under the same culture conditions.Moreover,the response time of LTCM for detection of practical samples was reduced from 6 to 4 h.At the same time,LTCM still retained very high sensitivity and specificity,while its lowest detection limit was 0.1 nM Cd^(2+)and the specificity was 23.29(compared to 0.1 nM and 17.55 in TCM,respectively).In summary,the positive and negative feedback cascade amplifiers effectively improved the performance of the biosensor LTCM,resulting in a greater linear response range,higher output signal intensity,and shorter response time than TCM while retaining comparable sensitivity and specificity,indicating better potential for practical applications.

An HGF-dependent positive feedback loop between bladder cancer cells and fibroblasts mediates lymphangiogenesis and lymphatic metastasis

Background Cancer-associated fibroblasts (CAFs) play a vital role in facilitating tumor progression through extensive reciprocal interplay with cancer cells. Tumor-derived extracellular vesicles (EVs) are the critical mediators involved in the crosstalk between cancer cells and stromal cells, contributing to the metastasis of cancers. Yet, the biological mechanisms of tumor-derived EVs in triggering CAFs phenotype to stimulate the lymph node (LN) metastasis of bladder cancer (BCa) are largely unknown. Here, we aimed to explore the effects and molecular mechanisms of tumor-derived EV-mediated CAFs phenotype in regulating BCa LN metastasis. Methods The high-throughput sequencing was utilized to identify the crucial long non-coding RNA (lncRNA) associated with CAF enrichment in BCa. The functional role of the transition of fibroblasts to CAFs induced by LINC00665-mediated EVs was investigated through the in vitro and in vivo assays. Chromatin isolation by RNA purification assays, fluorescence resonance energy transfer assays, cytokine profiling and patient-derived xenograft (PDX) model were performed to explore the underlying mechanism of LINC00665 in the LN metastasis of BCa. Results We found that CAFs are widely enriched in the tumor microenvironment of BCa, which correlated with BCa lymphangiogenesis and LN metastasis. We then identified a CAF-associated long non-coding RNA, LINC00665, which acted as a crucial mediator of CAF infiltration in BCa. Clinically, LINC00665 was associated with LN metastasis and poor prognosis in patients with BCa. Mechanistically, LINC00665 transcriptionally upregulated RAB27B expression and induced H3K4me3 modification on the promoter of RAB27B through the recruitment of hnRNPL. Moreover, RAB27B-induced EVs secretion endowed fibroblasts with the CAF phenotype, which reciprocally induced LINC00665 overexpression to form a RAB27B-HGF-c-Myc positive feedback loop, enhancing the lymphangiogenesis and LN metastasis of BCa. Importantly, we demonstrated that blocking EV-transmitted LINC00665 or HGF broke this loop and impaired BCa lymphangiogenesis in a PDX model. Conclusion Our study uncovers a precise mechanism that LINC00665 sustains BCa LN metastasis by inducing a RAB27B-HGF-c-Myc positive feedback loop between BCa cells and fibroblasts, suggesting that LINC00665 could be a promising therapeutic target for patients with LN metastatic BCa.

Meiotic nuclear divisions 1(MND1)fuels cell cycle progression by activating a KLF6/E2F1 positive feedback loop in lung adenocarcinoma

Background:Considering the increase in the proportion of lung adenocarcinoma(LUAD)cases among all lung cancers and its considerable contribution to cancer-related deaths worldwide,we sought to identify novel oncogenes to provide potential targets and facilitate a better understanding of the malignant progression of LUAD.Methods:The results from the screening of transcriptome and survival analyses according to the integrated Gene Expression Omnibus(GEO)datasets and The Cancer Genome Atlas(TCGA)data were combined,and a promising risk biomarker called meiotic nuclear divisions 1(MND1)was selectively acquired.Cell viability assays and subcutaneous xenograftmodelswere used to validate the oncogenic role ofMND1 in LUADcell proliferation and tumor growth.Aseries of assays,including mass spectrometry,co-immunoprecipitation(Co-IP),and chromatin immunoprecipitation(ChIP),were performed to explore the underlying mechanism.Results:MND1 up-regulation was identified to be an independent risk factor for overall survival in LUAD patients evaluated by both tissue microarray staining and third party data analysis.In vivo and in vitro assays showed that MND1 promoted LUAD cell proliferation by regulating cell cycle.The results of the Co-IP,ChIP and dual-luciferase reporter assays validated that MND1 competitively bound to tumor suppressor Kruppel-like factor 6(KLF6),and thereby protecting E2F transcription factor 1(E2F1)from KLF6-induced transcriptional repression.Luciferase reporter and ChIP assays found that E2F1 activated MND1 transcription by binding to its promoter in a feedback manner.Conclusions:MND1,KLF6,and E2F1 form a positive feedback loop to regulate cell cycle and confer DDP resistance in LUAD.MND1 is crucial for malignant progression and may be a potential therapeutic target in LUAD patients.

PLEK2 promotes cancer stemness and tumorigenesis of head and neck squamous cell carcinoma via the c-Myc-mediated positive feedback loop

Background:Head and neck squamous cell carcinoma(HNSCC)is one of the most frequent malignancies worldwide and is characterized by unfavorable prognosis,high lymph node metastasis and early recurrence.However,the molecular events regulating HNSCC tumorigenesis remain poorly understood.Therefore,uncovering the underlying mechanisms is urgently needed to identify novel and promising therapeutic targets for HNSCC.In this study,we aimed to explore the role of pleckstrin-2(PLEK2)in regulating HNSCC tumorigenesis.Methods:The expression pattern of PLEK2 and its clinical significance in HNSCC were determined by analyzing publicly assessable datasets and our own independent HNSCC cohort.In vitro and in vivo experiments,including cell proliferation,colony formation,Matrigel invasion,tumor sphere formation,ALDEFLUOR,Western blotting assays and xenograft mouse models,were used to investigate the role of PLEK2 in regulating the malignant behaviors of HNSCC cells.The underlying molecular mechanisms for the tumor-promoting role of PLEK2 were elucidated using co-immunoprecipitation,cycloheximide chase analysis,ubiquitination assays,chromatin immunoprecipitation-quantitative polymerase chain reaction,luciferase reporter assays and rescue experiments.Results:The expression levels of PLEK2 mRNA and protein were significantly increased in HNSCC tissues,and PLEK2 overexpression was strongly associated with poor overall survival and therapeutic resistance.Additionally,PLEK2 was important for maintaining the proliferation,invasion,epithelial-mesenchymal transition,cancer stemness and tumorigenesis of HNSCC cells and could alter the cellular metabolism of the cancer cells.Mechanistically,PLEK2 interacted with c-Myc and reduced the association of F-box and WD repeat domain containing 7(FBXW7)with c-Myc,thereby avoiding ubiquitination and subsequent proteasome-mediated degradation of c-Myc.Moreover,the c-Myc signaling activated by PLEK2 was important for sustaining the aggressive malignant phenotypes and tumorigenesis of HNSCC cells.c-Myc also directly bounded to the PLEK2 promoter and activated its transcription,forming a positive feedback loop.Conclusions:Collectively,these findings uncover a previously unknown molecular basis of PLEK2-enhanced c-Myc signaling in HNSCC,suggesting that PLEK2 may represent a promising therapeutic target for treating HNSCC.

Asymmetries of Positive Feedback Trading in Individual Stocks:Evidences from China

The paper investigates the positive feedback trading phenomenon of individual stocks in China’s stock market.Heterogeneous regression models and a non-parametric Sentana-Wadhwani model are developed and applied to prove the existence of rise-favor asymmetric feedback trading.This result is consistent with the reality of China’s stock market,that is,the herding behavior of irrational retail investors has a significant influence on the market.However,the result is in contrast with the stylized findings using market indices.Further empirical research shows that this distinction could be explained by the average effect of indices.The asymmetries of positive feedback trading caused by heterogeneous feedback trading and the uneven distributed retail trading in individual stocks,are covered by the weighted average effect in indices.Therefore,the indices exhibit a different asymmetric pattern from that of individual stocks.

Black Phosphorus Nanosheets Enhance Photophosphorylation by Positive Feedback

We construct a natural-artificial hybrid architecture containing black phosphorus nanosheets(BPNS)to enhance photosynthesis of chloroplast in a positive-feedback manner.In this architecture,oxygen yielded by photosynthesis during water splitting by photosystemⅡpromotes the photoreaction of BPNS to produce proton and inorganic phosphate(Pi).Further,transmembrane proton gradient is increased to drive ATP synthase to synthesize ATP.Meanwhile,additional photogenerated electrons produced by BPNS are transferred to the photosynthesis process.As a consequence,photosynthesis performed by chloroplast is improved.Quantitatively,photophosphorylation efficacy of the hybrid system is increased by 1.89 times in the case of Pi deficiency.This work offers a new path to enhance solar-to-chemical energy conversion,holding promise in boosting natural photosynthesis.

Positive feedback between retinoic acid and 2-phospho-L-ascorbic acid trisodium salt during somatic cell reprogramming

Retinoic acid(RA)and 2-phospho-L-ascorbic acid trisodium salt(AscPNa)promote the reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells.In the current studies,the lower abilities of RA and AscPNa to promote reprogramming in the presence of each other suggested that they may share downstream pathways at least partially.The hypothesis was further supported by the RNA-seq analysis which demonstrated a high-level overlap between RA-activated and AscPNa activated genes during reprogramming.In addition,RA upregulated Glut1/3,facilitated the membrane transportation of dehydroascorbic acid,the oxidized form of L-ascorbic acid,and subsequently maintained intracellular L-ascorbic acid at higher level and for longer time.On the other hand,AscPNa facilitated the mesenchymal-epithelial transition during reprogramming,downregulated key mesenchymal transcriptional factors like Zeb1 and Twist1,subsequently suppressed the expression of Cyp26a1/b1 which mediates the metabolism of RA,and sustained the intracellular level of RA.Furthermore,the different abilities of RA and AscPNa to induce mesenchymal-epithelial transition,pluripotency,and neuronal differentiation explain their complex contribution to reprogramming when used individually or in combination.Therefore,the current studies identified a positive feedback between RA and AscPNa,or possibility between vitamin A and C,and further explored their contributions to reprogramming.

Power Quality Assessment of Voltage Positive Feedback Based Islanding Detection Algorithm

Islanding refers to a condition where distributed generators(DGs)inject power solely to the local load after electrical separation from power grid.Several islanding detection methods(IDMs)categorized into remote,active,and passive groups have been reported to detect this undesirable state.In active techniques,a disturbance is injected into the DG’s controller to drift a local yardstick out of the permissible range.Although this disturbance leads to more effective detections even in well-balanced island,it raises the total harmonic distortion(THD)of the output current under the normal operation conditions.This paper analyzes the power quality aspect of the modified sliding mode controller as a new active IDM for grid-connected photovoltaic system(GCPVS)with a string inverter.Its performance is compared with the voltage positive feedback(VPF)method,a well-known active IDM.This evaluation is carried out for a 1 k Wp GCPVS in MATLAB/Simulink platform by measuring the output current harmonics and THD as well as the efficiency under various penetration and disturbance levels.The output results demonstrate that since the proposed disturbance changes the amplitude of the output current,it does not generate harmonics/subharmonics.Thereby,it has a negligible adverse effect on power quality.It is finally concluded that the performance of the sliding mode-based IDM is reliable from the standpoints of islanding detection and power quality.

Neuronal insulin receptor mediates a positive feedback regulation of insulin biosynthesis in Drosophila

Insulin signaling is vital for the development and growth of mammals,which also plays a prominent role in the regulation of metabolism,stress response,reproduction,and behaviors.Therefore,insulin signaling is under tight regulation at the levels of insulin biosynthesis,secretion,and post-secretion action(Melloul et al.,2002;Na€ssel et al.,2013).Drosophila insulin-like peptides(DILPs)

Flow Control for a Two-Stage Proportional Valve with Hydraulic Position Feedback

The current research mainly focuses on the flow control for the two-stage proportional valve with hydraulic position feedback which is named as Valvistor valve.Essentially,the Valvistor valve is a proportional throttle valve and the flow fluctuates with the change of load pressure.The flow fluctuation severely restricts the application of the Valvistor valve.In this paper,a novel flow control method the Valvistor valve is provided to suppress the flow fluctuation and develop a high performance proportional flow valve.The mathematical model of this valve is established and linearized.Fuzzy proportional-integral-derivative(PID)controller is adopted in the closed-loop flow control system.The feedback is obtained by the flow inference with back-propagation neural network(BPNN)based on the spool displacement in the pilot stage and the pressure differential across the main orifice.The results show that inference with BPNN can obtain the flow data fast and accurately.With the flow control method,the flow can keep at the set point when the pressure differential across the main orifice changes.The flow control method is effective and the Valvistor valve changes from proportional throttle valve to proportional flow valve.For the developed proportional flow valve,the settling time of the flow is very short when the load pressure changes abruptly.The performances of hysteresis,linearity and bandwidth are in a high range.The linear mathematical model can be verified and the assumptions in the system modeling is reasonable.

Roles of Equatorial Ocean Currents in Sustaining the Indian Ocean Dipole Peak

In this study,on the basis of the results of the European Centre for Medium-Range Weather Forecasts Ocean Reanalysis System 4,the response of equatorial ocean currents and their roles during the peak phase of the Indian Ocean Dipole(IOD)are comprehensively explored.During the IOD peak season,a series of ocean responses emerge.First,significant meridional divergence in the surface layer and convergence in the subsurface layer are found in the equatorial region.The equatorial easterly winds and offequatorial wind curl anomalies are found to be responsible for the divergence at 55°–80°E and the convergence at 70°–90°E.Second,the meridional divergence and convergence are found to favor a weakened Wyrtki jet(WJ)in the surface layer and an enhanced Equatorial Undercurrent(EUC)in the subsurface layer,respectively.Therefore,these ocean responses provide ocean positive feedback that sustains the IOD peak as the weakened WJ and enhanced EUC help maintain the zonal temperature gradient.Additionally,heat budget analyses indicate that the weakened WJ favors sea surface temperature anomaly warming in the western Indian Ocean,whereas the enhanced EUC maintains the sea surface temperature anomaly cooling in the eastern Indian Ocean.

Understanding Interannual Variations of the Local Rainy Season over the Southwest Indian Ocean

Located at the southern boundary of the tropical rainfall belt within the South Africa monsoon regime,Rodrigues Island,~2500 km east of East Africa,is ideally located to investigate climatic changes over the southwest Indian Ocean(SWIO).In this study,we investigate the climatic controls of its modern interannual rainfall variability in terms of teleconnection and local effects.We find that increased rainfall over the SWIO tends to occur in association with anomalously warm(cold)SSTs over the equatorial central Pacific(Maritime Continent),resembling the central Pacific El Niño,closely linked with the Victoria mode in the North Pacific.Our analyses show that the low-level convergence induced by warm SST over the equatorial central Pacific leads to anomalous low-level divergence over the Maritime Continent and convergence over a large area surrounding the Rodrigues Island,which leads to increased rainfall over the SWIO during the rainy season.Meanwhile,the excited Rossby wave along the tropical Indian Ocean transports more water vapor from the tropical convergence zone into the SWIO via intensified northwest wind.Furthermore,positive feedback induced by the Rossby wave response to the increased rainfall in the region contributes to the large interannual variations over the SWIO.

Analysis of thermal-mechanical coupled characteristics of vehicle twin-tube shock absorber

A comprehensive model that included mechanical dynamics of the shock absorber coupled with its thermal properties was proposed innovatively.Moreover a thermal-mechanical coupled model which reflected the closed-loop positive feedback system was established by using MATLAB/SIMULINK,and some curves of shock absorber temperature rising characteristic were obtained by simulation &computation under several operating modes and different parameters conditions.Research results show that:shock absorber design parameters,external excitations,and thermo-physical properties parameter,such as oil density have effect on the shock absorber temperature rising characteristic.However other thermo-physical properties parameters,such as oil specific heat,cylinder density,cylinder specific heat,and cylinder thermal conductivity,have no effect on it.The results may be used for studying reliability design of the shock absorber.

Position and Velocity Time Delay for Suppression Vibrations of a Hybrid Rayleigh-Van der Pol-Duffing Oscillator

In this paper,we used time delay feedback to minimize the vibrations of a hybrid Rayleigh–van der Pol–Duffing oscillator.This system is a one-degree-offreedom containing the cubic and fifth nonlinear terms and an external force.We applied the multiple scales method to get the solution from first approximation.Graphically and numerically,we studied the system before and after adding time delay feedback at the primary resonance case(
ffi!).We used MATLAB program to simulate the efficacy of different parameters and the time delay on the main system.

A Suitable Active Control for Suppression the Vibrations of a Cantilever Beam

In our consideration,a comparison between four different types of controllers for suppression the vibrations of the cantilever beam excited by an external force is carried out.Those four types are the linear velocity feedback control,the cubic velocity feedback control,the non-linear saturation controller(NSC)and the positive position feedback(PPF)controller.The suitable type is the PPF controller for suppression the vibrations of the cantilever beam.The approximate solution obtained up to the first approximation by using the multiple scale method.The PPF controller effectiveness is studied on the system.We used frequency-response equations to investigate the stability of a cantilever beam.We notified that,there is a good agreement between the analytical solution and the numerical solution.

A Study on the Molecular Switch of Gene Expression of the Mouse Heart Nuclear DNA Fragments

It is observed by in situ stain that LDH (1 5) ...nNAD + can probably enter the nucleopore and can be bound bound specifically with the genes that encode them. During the in vitro expression, the dilution of heart nuclear DNA fragments could enhance the expression activity of LDH/DNA and the amount of expressed LDH (1 5) is in proportion to the amount of dissociable LDH (1 5) on the LDH/DNA. With the integration of 14C Leu to the proteins, it is also observed that the addition of LDH (1 5) ...nNAD + can suppress the in vitro expression activity of LDH/DNA. AFM observation shows that the regulation sequence at the both ends of active genes may be bound with such active factors as proteins encoded by the genes which probably is the main molecular switch of gene expression and regulation we have been always searching for. Our work shows the prospective application of the combination of AFM and isotope labeling in the research of biological reaction.

Gene Expression Pattern of Histone Acetylation Enzymes Changed in the Hypothalamus of Middle-Aged Female Rats:A Putative Mechanism for Female Reproductive Aging

Objective:Female reproductive aging is characterized by reduced responsiveness of the hypothalamus to E2-positive feedback,which can result in alterations of gene expression and luteinizing hormone(LH)surge dysfunction.We hypothesize that age-related changes in E2-responsive gene expression are due to altered histone acetylation by histone deacetylases(HDACs)or estrogen receptor-alpha(ERα)coactivators with histone acetyltransferase(HAT)activity.Methods:In the present study,young and middle-aged female rats were ovariectomized(OVX)and treated with E2 or oil once per day for 2 days.At the time of the expected LH surge,the anterior and posterior hypothalami were dissected,and gene expression of 11 HDACs and 4 ERαcoactivators with HAT activity was measured using real-time polymerase chain reaction.Results:In the anterior hypothalamus,age affected the gene expression of 3 HDACs(Hdac3,Hdac5,and Hdac11)and 2 ERαcoactivators(Src2 and Crebbp).E2 treatment significantly decreased mRNA levels of 4 HDACs(Hdac4,Hdac5,Hdac10,and Hdac11)and 2 ERαcoactivators(Src2 and Crebbp)in young females(3-4 months).However,none of the genes responded to E2 in the middle-aged females(9-11 months),except Hdac10.In the posterior hypothalamus,age influenced Hdac5 and Src1 mRNA expression.E2 treatment increased Hdac4 and Crebbp mRNA levels in the young but not middle-aged females.Conclusions:These data suggest that E2 regulates HDACs and ERαcoactivators with HAT activity in an age-and E2-dependent manner,which may contribute to the age-related gene expression changes on the day of LH surge in female reproductive aging.

A"three musketeers"tactic for inclining interferon-γ as a comrade-in-arm to reinforce the synergistic-tumoricidal therapy

Interferon-γ(IFN-γ),secreted by activated T cells predominantly,plays a crucial performance in the tumoricidal immune response.Unfortunately,a high level of IFN-γseverely ignites the immunosuppressive response,especially by increasing the expression of immune checkpoint programmed death-ligand 1(PD-L1)and immunoregulatory enzyme indoleamine 2,3-dioxygenase 1(IDO-1).Herein,we have explored a versatile IFN-γ-nano-integrator(aPD-L1-SH@Ce6@NLG919-PEG,simplified as CNDP)to establish a positive anti-tumor feedback loop to amplify the IFN-γ-mediated tumoricidal effect.In this nanointegrator,photosensitizer chlorin e6(Ce6)mediates photodynamic therapy(PDT)to re-shape immunogenicity and activate the adaptive immune response,followed by the secretion of high-level IFN-γto struggle tumor cells.IDO-1 inhibitor(NLG919)afterwards mitigates the immunosuppressive behavior of IFN-γby neutralizing the function of IDO-1.To turn“waste”into wealth,anti-PD-L1(aPD-L1)antibodies are technically integrated into the nano-integrator to propel the precise attack of breast cancer through ascending PD-L1 blockade.Together,this“three musketeers”nano-integrator tumoricidal tactic may give a unique insight into the clinical anti-tumor therapy.