Spontaneous rupture of hepatocellular carcinoma(HCC) is a life-threatening complication and its prognosis is significantly poor because of the high recurrence rate after initial hepatectomy. Resection of isolated extr...Spontaneous rupture of hepatocellular carcinoma(HCC) is a life-threatening complication and its prognosis is significantly poor because of the high recurrence rate after initial hepatectomy. Resection of isolated extrahepatic metastasis of HCC has been advocated to obtain a possibility of long-term survival. However, it is a challenge for clinicians to detect implantation metastasis of spontaneously ruptured HCC. Accurate re-staging plays the most important role in making a decision on isolated metastasis resection. 18F-fluorodeoxyglucose(18F-FDG) positron emission tomography/computed tomography(PET/CT) is useful in detecting intraabdominal implantation metastasis from a variety of malignancies and shows superior accuracy to conventional imaging modalities in determining the location of metastasis. We present one patient with a new isolated pelvic implantation metastasis detected by 18F-FDG PET/CT and pathologically confirmed by PET/CT-guided percutaneous biopsy, who had a history of resection of spontaneously ruptured HCC two years ago. The patient's condition was stable at the 6-mo follow-up after resection of the isolated pelvic metastasis.展开更多
OBJECTIVE: To evaluate the effects of Taichong(LR3) acupuncture points(acupoints) on the expression of glucose transporter protein 1(GLUT1) in the hypothalamus of spontaneously hypertensive rats(SHRs) as measured by c...OBJECTIVE: To evaluate the effects of Taichong(LR3) acupuncture points(acupoints) on the expression of glucose transporter protein 1(GLUT1) in the hypothalamus of spontaneously hypertensive rats(SHRs) as measured by combined positron emission tomography and computed tomography(PETCT).METHODS: Spontaneously hypertensive rats(SHR)were divided into model, Taichong(LR 3) acupuncture, and sham groups. Additionally, Tokyo Wistar rats were used as the control group. Changes in blood pressure were recorded in different groups of rats before and after the corresponding treatment. Hematoxylin and eosin(HE) staining was used to study basic morphological changes, and immunohistochemistry was used to determine GLUT1 expression in the hypothalamus. Further,PET-CT was utilized to elucidate the antihypertensive mechanism after acupuncture at the Taichong(LR 3) acupoints.RESULTS: PET-CT indicated activation of the hypothalamus. Measurement of blood pressure showed that acupuncture at the Taichong(LR 3) acupoints lowered blood pressure. HE staining did not show any significant pathological changes, although differences in cell number were observed. Immunohistochemical analysis indicated a GLUT1 downregulation in the SHRs of the Taichong(LR 3) acupuncture group after the treatment.CONCLUSION: Acupuncture at Taichong(LR 3) acupoints lowered blood pressure in SHRs, with possible mechanisms being changes in cell number and GLUT1 expression in the hypothalamus.展开更多
Hypertension is currently one of the most prevalent il nesses worldwide,and is the second most common cause of heart failure,only behind ischemic cardiomyopathy.The development of novel multimodality imaging technique...Hypertension is currently one of the most prevalent il nesses worldwide,and is the second most common cause of heart failure,only behind ischemic cardiomyopathy.The development of novel multimodality imaging techniques in recent years has broadened the diagnostic methods,risk stratification and monitoring of treatment of cardiovascular diseases available for clinicians.Cardiovascular magnetic resonance(CMR) has a great capacity to evaluate cardiac dimensions and ventricular function,is extremely useful in ruling-out ischemic cardiomyopathy,the evaluation of the vascular system,in making the differential diagnosis for resistant hypertension and risk stratification for hypertensive cardiomyopathy and constitutes today,the method of choice to evaluate left ventricular systolic function.Computed tomography(CT) is the method of choice for the evaluation of vascular anatomy,including coronary arteries,and is also able to provide both functional and structural information.Finally,nuclear cardiology studies have been traditionally used to evaluate myocardial ischemia,along with offering the capacity to evaluate ventricular,endothelial and cardiac innervation function;information that is key in directing the treatment of the patient.In this narrative review,the most recent contributions of multimodality imaging to the patient with hypertension(CMR,CT and nuclear cardiology) will be reviewed.展开更多
OBJECTIVE: To investigate antihypertensive effect in rats in order to confirm that twirling reinforcing-reducing manipulation(TRRM) might be the central mechanism underlying the action.METHODS: In the study,^(18) F-2-...OBJECTIVE: To investigate antihypertensive effect in rats in order to confirm that twirling reinforcing-reducing manipulation(TRRM) might be the central mechanism underlying the action.METHODS: In the study,^(18) F-2-fluoro-deoxy-D-glucose positron emission tomography(18 F-FDG-PET)was employed. Fifity-six spontaneous hypertensive rats(SHRs) were randomly divided into a model group, a single-needle acupuncture(SNA) group, a twirling reinforcing group(SNA + TRF) and a twirling reducing(SNA + TRD) group. Fourteen Wistar rats were assigned to the control group. The acupuncture intervention at Taichong(LR 3) acupoint was administered once daily in the SNA, SNA + TRF and SNA + TRD groups for 14 days, with 1 d interval between the two weeks. The blood pressure(BP) of all rats was measured repeatedly and18 F-FDG-PET scans were conducted on the 14 th day. PET images were processed with Statistical Parametric Mapping 8.0.RESULTS: After the intervention, systolic BP showed a significant decrease in the SNA, SNA+TRF and SNA + TRD versus the model groups(all P <0.01) and in the SNA + TRF and SNA + TRD versus the SNA groups(both P < 0.01), with the SNA+TRD group exhibited the best antihypertensive effect(P < 0.01). The key brain regions activated by TRRM were mainly concentrated in the cerebellum, hippocampus, hypothalamus, medulla oblongata, insular cortex, midbrain, thalamus and visual cortex.CONCLUTION: TRRM could significantly lower the BP of SHRs by improving the cerebral glucose metabolism of the activated key brain regions and the underlying central mechanism may be related to the central rennin-angiotensin system and neurotransmission.展开更多
目的:利用18脱氧葡萄糖正电子发射断层扫描和计算机断层扫描(PET-CT)技术,探究针刺人迎穴对自发性高血压(SHR)大鼠脑功能区葡萄糖代谢的影响。方法:将30只SHR大鼠(BP≥140 mm Hg)测量血压后随机分为模型组、人迎组、非穴针灸组,每组10只...目的:利用18脱氧葡萄糖正电子发射断层扫描和计算机断层扫描(PET-CT)技术,探究针刺人迎穴对自发性高血压(SHR)大鼠脑功能区葡萄糖代谢的影响。方法:将30只SHR大鼠(BP≥140 mm Hg)测量血压后随机分为模型组、人迎组、非穴针灸组,每组10只;以10只同等体质量的WKY大鼠作为对照组。人迎组和非穴针灸组针刺30 min,模型组和对照组不进行干预,然后使用PET-CT扫描采集大鼠脑功能图像,得到的图像用SPM8处理。结果:与模型组比较,人迎组大鼠被灭活的脑区有小脑、初级视觉皮层、次级视觉皮质和穹窿下器官等部位;被激活的脑区有嗅球、小脑、第Ⅳ脑室、蓝斑核、前庭内侧核、下丘脑外侧核。针刺人迎穴对于提升大脑葡萄糖代谢的作用比非穴针灸更明显。结论:针刺人迎穴能显著降低SHR的血压,针刺人迎穴并不是特异性的激活特定脑区,而是对SHR病理状态下正负激活的多个脑区发挥双向良性调节作用。展开更多
目的:全自动一体化合成水甘油通道蛋白分子探针^(11)C-N-甲基-3-氨基-1,2-丙二醇,并用以大鼠肝脏PET/CT显像。方法:使用GE TRACERlab FX C Pro合成平台合成^(11)C-N-甲基-3-氨基-1,2-丙二醇后,对Wistar大鼠进行^(11)C-N-甲基-3-氨基-1,2...目的:全自动一体化合成水甘油通道蛋白分子探针^(11)C-N-甲基-3-氨基-1,2-丙二醇,并用以大鼠肝脏PET/CT显像。方法:使用GE TRACERlab FX C Pro合成平台合成^(11)C-N-甲基-3-氨基-1,2-丙二醇后,对Wistar大鼠进行^(11)C-N-甲基-3-氨基-1,2-丙二醇动态扫描,观察肝脏放射性摄取及随时间变化情况,绘制时间-放射性活度曲线。结果 :^(11)C-N-甲基-3-氨基-1,2-丙二醇合成效率>50%,放射性化学纯度>98%,合成时间30 min左右。PET/CT肝脏显像清楚,且随时间延长放射性摄取明显减低。结论:全自动一体化合成^(11)C-N-甲基-3-氨基-1,2-丙二醇方法简单、纯度高,可用于大鼠肝脏方面临床前期研究。展开更多
Hypothermia is an important protective strategy against global cerebral ischemia following cardiac arrest.However,the mechanisms of hypothermia underlying the changes in different regions and connections of the brain ...Hypothermia is an important protective strategy against global cerebral ischemia following cardiac arrest.However,the mechanisms of hypothermia underlying the changes in different regions and connections of the brain have not been fully elucidated.This study aims to identify the metabolic nodes and connection integrity of specific brain regions in rats with global cerebral ischemia that are most affected by hypothermia treatment.18F-fluorodeoxyglucose positron emission tomography was used to quantitatively determine glucose metabolism in different brain regions in a rat model of global cerebral ischemia established at 31–33℃.Diffusion tensor imaging was also used to reconstruct and explore the brain connections involved.The results showed that,compared with the model rats established at 37–37.5℃,the rat models of global cerebral ischemia established at 31–33℃had smaller hypometabolic regions in the thalamus and primary sensory areas and sustained no obvious thalamic injury.Hypothermia selectively preserved the integrity of the anterior forebrain mesocircuit,exhibiting protective effects on the brain during the global cerebral ischemia.The study was approved by the Institutional Animal Care and Use Committee at Capital Medical University(approval No.XW-AD318-97-019)on December 15,2019.展开更多
基金Supported by National Science Foundation for Yong Scholars of China,No.81101067
文摘Spontaneous rupture of hepatocellular carcinoma(HCC) is a life-threatening complication and its prognosis is significantly poor because of the high recurrence rate after initial hepatectomy. Resection of isolated extrahepatic metastasis of HCC has been advocated to obtain a possibility of long-term survival. However, it is a challenge for clinicians to detect implantation metastasis of spontaneously ruptured HCC. Accurate re-staging plays the most important role in making a decision on isolated metastasis resection. 18F-fluorodeoxyglucose(18F-FDG) positron emission tomography/computed tomography(PET/CT) is useful in detecting intraabdominal implantation metastasis from a variety of malignancies and shows superior accuracy to conventional imaging modalities in determining the location of metastasis. We present one patient with a new isolated pelvic implantation metastasis detected by 18F-FDG PET/CT and pathologically confirmed by PET/CT-guided percutaneous biopsy, who had a history of resection of spontaneously ruptured HCC two years ago. The patient's condition was stable at the 6-mo follow-up after resection of the isolated pelvic metastasis.
基金National Key Basic Research Program of China(973 Program):Basic Research on the Specificity of the Meridian Effect and the Key Influencing Factors(No.2012CB518504)
文摘OBJECTIVE: To evaluate the effects of Taichong(LR3) acupuncture points(acupoints) on the expression of glucose transporter protein 1(GLUT1) in the hypothalamus of spontaneously hypertensive rats(SHRs) as measured by combined positron emission tomography and computed tomography(PETCT).METHODS: Spontaneously hypertensive rats(SHR)were divided into model, Taichong(LR 3) acupuncture, and sham groups. Additionally, Tokyo Wistar rats were used as the control group. Changes in blood pressure were recorded in different groups of rats before and after the corresponding treatment. Hematoxylin and eosin(HE) staining was used to study basic morphological changes, and immunohistochemistry was used to determine GLUT1 expression in the hypothalamus. Further,PET-CT was utilized to elucidate the antihypertensive mechanism after acupuncture at the Taichong(LR 3) acupoints.RESULTS: PET-CT indicated activation of the hypothalamus. Measurement of blood pressure showed that acupuncture at the Taichong(LR 3) acupoints lowered blood pressure. HE staining did not show any significant pathological changes, although differences in cell number were observed. Immunohistochemical analysis indicated a GLUT1 downregulation in the SHRs of the Taichong(LR 3) acupuncture group after the treatment.CONCLUSION: Acupuncture at Taichong(LR 3) acupoints lowered blood pressure in SHRs, with possible mechanisms being changes in cell number and GLUT1 expression in the hypothalamus.
文摘Hypertension is currently one of the most prevalent il nesses worldwide,and is the second most common cause of heart failure,only behind ischemic cardiomyopathy.The development of novel multimodality imaging techniques in recent years has broadened the diagnostic methods,risk stratification and monitoring of treatment of cardiovascular diseases available for clinicians.Cardiovascular magnetic resonance(CMR) has a great capacity to evaluate cardiac dimensions and ventricular function,is extremely useful in ruling-out ischemic cardiomyopathy,the evaluation of the vascular system,in making the differential diagnosis for resistant hypertension and risk stratification for hypertensive cardiomyopathy and constitutes today,the method of choice to evaluate left ventricular systolic function.Computed tomography(CT) is the method of choice for the evaluation of vascular anatomy,including coronary arteries,and is also able to provide both functional and structural information.Finally,nuclear cardiology studies have been traditionally used to evaluate myocardial ischemia,along with offering the capacity to evaluate ventricular,endothelial and cardiac innervation function;information that is key in directing the treatment of the patient.In this narrative review,the most recent contributions of multimodality imaging to the patient with hypertension(CMR,CT and nuclear cardiology) will be reviewed.
基金Supported by the National Natural Science Foundation of China(Use PET Brain Imaging Technology to Study the Acupuncture Manipulation of Twirling Reinforcing-Reducing Regulation in Spontaneously Hypertensive Rats by Central Integration Signaling Network Construction,No.8157150840)
文摘OBJECTIVE: To investigate antihypertensive effect in rats in order to confirm that twirling reinforcing-reducing manipulation(TRRM) might be the central mechanism underlying the action.METHODS: In the study,^(18) F-2-fluoro-deoxy-D-glucose positron emission tomography(18 F-FDG-PET)was employed. Fifity-six spontaneous hypertensive rats(SHRs) were randomly divided into a model group, a single-needle acupuncture(SNA) group, a twirling reinforcing group(SNA + TRF) and a twirling reducing(SNA + TRD) group. Fourteen Wistar rats were assigned to the control group. The acupuncture intervention at Taichong(LR 3) acupoint was administered once daily in the SNA, SNA + TRF and SNA + TRD groups for 14 days, with 1 d interval between the two weeks. The blood pressure(BP) of all rats was measured repeatedly and18 F-FDG-PET scans were conducted on the 14 th day. PET images were processed with Statistical Parametric Mapping 8.0.RESULTS: After the intervention, systolic BP showed a significant decrease in the SNA, SNA+TRF and SNA + TRD versus the model groups(all P <0.01) and in the SNA + TRF and SNA + TRD versus the SNA groups(both P < 0.01), with the SNA+TRD group exhibited the best antihypertensive effect(P < 0.01). The key brain regions activated by TRRM were mainly concentrated in the cerebellum, hippocampus, hypothalamus, medulla oblongata, insular cortex, midbrain, thalamus and visual cortex.CONCLUTION: TRRM could significantly lower the BP of SHRs by improving the cerebral glucose metabolism of the activated key brain regions and the underlying central mechanism may be related to the central rennin-angiotensin system and neurotransmission.
文摘目的:利用18脱氧葡萄糖正电子发射断层扫描和计算机断层扫描(PET-CT)技术,探究针刺人迎穴对自发性高血压(SHR)大鼠脑功能区葡萄糖代谢的影响。方法:将30只SHR大鼠(BP≥140 mm Hg)测量血压后随机分为模型组、人迎组、非穴针灸组,每组10只;以10只同等体质量的WKY大鼠作为对照组。人迎组和非穴针灸组针刺30 min,模型组和对照组不进行干预,然后使用PET-CT扫描采集大鼠脑功能图像,得到的图像用SPM8处理。结果:与模型组比较,人迎组大鼠被灭活的脑区有小脑、初级视觉皮层、次级视觉皮质和穹窿下器官等部位;被激活的脑区有嗅球、小脑、第Ⅳ脑室、蓝斑核、前庭内侧核、下丘脑外侧核。针刺人迎穴对于提升大脑葡萄糖代谢的作用比非穴针灸更明显。结论:针刺人迎穴能显著降低SHR的血压,针刺人迎穴并不是特异性的激活特定脑区,而是对SHR病理状态下正负激活的多个脑区发挥双向良性调节作用。
文摘目的:全自动一体化合成水甘油通道蛋白分子探针^(11)C-N-甲基-3-氨基-1,2-丙二醇,并用以大鼠肝脏PET/CT显像。方法:使用GE TRACERlab FX C Pro合成平台合成^(11)C-N-甲基-3-氨基-1,2-丙二醇后,对Wistar大鼠进行^(11)C-N-甲基-3-氨基-1,2-丙二醇动态扫描,观察肝脏放射性摄取及随时间变化情况,绘制时间-放射性活度曲线。结果 :^(11)C-N-甲基-3-氨基-1,2-丙二醇合成效率>50%,放射性化学纯度>98%,合成时间30 min左右。PET/CT肝脏显像清楚,且随时间延长放射性摄取明显减低。结论:全自动一体化合成^(11)C-N-甲基-3-氨基-1,2-丙二醇方法简单、纯度高,可用于大鼠肝脏方面临床前期研究。
基金supported by Beijing Municipal Health Commission of China,No.Jing2019-2(to TLW)。
文摘Hypothermia is an important protective strategy against global cerebral ischemia following cardiac arrest.However,the mechanisms of hypothermia underlying the changes in different regions and connections of the brain have not been fully elucidated.This study aims to identify the metabolic nodes and connection integrity of specific brain regions in rats with global cerebral ischemia that are most affected by hypothermia treatment.18F-fluorodeoxyglucose positron emission tomography was used to quantitatively determine glucose metabolism in different brain regions in a rat model of global cerebral ischemia established at 31–33℃.Diffusion tensor imaging was also used to reconstruct and explore the brain connections involved.The results showed that,compared with the model rats established at 37–37.5℃,the rat models of global cerebral ischemia established at 31–33℃had smaller hypometabolic regions in the thalamus and primary sensory areas and sustained no obvious thalamic injury.Hypothermia selectively preserved the integrity of the anterior forebrain mesocircuit,exhibiting protective effects on the brain during the global cerebral ischemia.The study was approved by the Institutional Animal Care and Use Committee at Capital Medical University(approval No.XW-AD318-97-019)on December 15,2019.
