The influences of exogenous H 2O 2 and salicylic acid (SA) treatments on the alternative respiratory pathway (ARP) in aged potato (Solanum tuberosum L.) tuber slices were compared. The results showed that both H 2O...The influences of exogenous H 2O 2 and salicylic acid (SA) treatments on the alternative respiratory pathway (ARP) in aged potato (Solanum tuberosum L.) tuber slices were compared. The results showed that both H 2O 2(5.0 mmol/L) and SA (0.1 mmol/L) treatments had a significant inducing effect on ARP capacity (V alt ) and its ratio to total respiration (V alt /V t) in potato slices aged for 24 h. With a monoclonal antibody against the alternative oxidase (AOX), Western blotting results showed that both H 2O 2 and SA treatments increased the AOX expression levels in aged potato tuber slices. However, the results of oxygen isotope discrimination experiments showed that H 2O 2 had no influence on the in vivo ARP activity (ρV alt ) and its contribution to V t(expressed as ρV alt /V t) in potato slices aged for 24 h, but SA had a significant influence on the ρV alt and ρV alt /V t values of the aged potato tuber slices. These results indicate that there are differences between the effects of H 2O 2 and SA on ARP in plant tissues. Both of them possess the ability to induce ARP capacity through inducing AOX expression. However, SA can simultaneously stimulate the operation of ARP, but H 2O 2 can not.展开更多
采用自制脉冲电场(Pulsed electric field:PEF)装置,研究了不同脉冲频率和电场强度预处理对马铃薯片微波干燥特性的影响.设计了3因素(脉冲频率、电场强度、微波功率密度)2指标(脱水速率和厚度比)二次回归正交组合试验.对试验结果采用SPS...采用自制脉冲电场(Pulsed electric field:PEF)装置,研究了不同脉冲频率和电场强度预处理对马铃薯片微波干燥特性的影响.设计了3因素(脉冲频率、电场强度、微波功率密度)2指标(脱水速率和厚度比)二次回归正交组合试验.对试验结果采用SPSS软件进行回归分析,得出PEF预处理后马铃薯片微波干燥动力学方程和2指标回归方程.结果证明,经PEF预处理后马铃薯片微波干燥速率快于未经预处理的马铃薯样品;马铃薯微波干燥方程分段适用Page模型;试验验证了干燥动力学方程和回归方程的预测性都较好(p<0.05).采用MATLAB软件对2指标回归方程进行综合优化,得到可供参考的脉冲电场预处理马铃薯片微波干燥工艺参数,即脉冲频率、电场强度、微波功率密度分别为30 Hz、1.0 kV/cm、1.0 W/g.展开更多
文摘The influences of exogenous H 2O 2 and salicylic acid (SA) treatments on the alternative respiratory pathway (ARP) in aged potato (Solanum tuberosum L.) tuber slices were compared. The results showed that both H 2O 2(5.0 mmol/L) and SA (0.1 mmol/L) treatments had a significant inducing effect on ARP capacity (V alt ) and its ratio to total respiration (V alt /V t) in potato slices aged for 24 h. With a monoclonal antibody against the alternative oxidase (AOX), Western blotting results showed that both H 2O 2 and SA treatments increased the AOX expression levels in aged potato tuber slices. However, the results of oxygen isotope discrimination experiments showed that H 2O 2 had no influence on the in vivo ARP activity (ρV alt ) and its contribution to V t(expressed as ρV alt /V t) in potato slices aged for 24 h, but SA had a significant influence on the ρV alt and ρV alt /V t values of the aged potato tuber slices. These results indicate that there are differences between the effects of H 2O 2 and SA on ARP in plant tissues. Both of them possess the ability to induce ARP capacity through inducing AOX expression. However, SA can simultaneously stimulate the operation of ARP, but H 2O 2 can not.
